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ALCHEMY Jurnal Penelitian Kimia
Published by Universitas Sebelas Maret
ISSN : 14124092     EISSN : 24434183     DOI : -
Core Subject : Science, Social, Engineering,
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology
ALCHEMY Jurnal Penelitian Kimia is a chemistry journal published by Sebelas Maret University, Surakarta. ALCHEMY Jurnal Penelitian Kimia publishes original research articles or review articles in organic chemistry, inorganic chemistry, analytical chemistry, physical chemistry, biochemistry, and environmental chemistry.
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Articles 13 Documents
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Search results for , issue "Vol 13, No 2 (2017): September" : 13 Documents clear
APPLICATION OF HPLC-BASED METABOLOMICS TO IDENTIFY RETENTION TIMES ANTIBACTERIAL COMPONENTS OF Stapylococcus aureus FROM KECOMBRANG FLOWER EXTRACT (Etlingera elatior) Wahyu Haryati Maser; Nancy Dewi Yuliana
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.2.4890.239-249

Abstract

Kecombrang (Etlingera elatior) is a well-known herb in Indonesia that is widely used in traditional medicine and as a flavour in local dishes. The aim of this study was to identify retention time of antibacterial components from kecombrang flower extract (Etlingera elatior) by HPLC-based metabolomics method. The antibacterial activity of ethanol extracts, chloroform, and water fractions was assessed by well-diffusion agar method against Staphylococcus aureus. Only chloroform fraction showed medium inhibition activity against Staphylococcus aureus. The HPLC chemical profiles of the fractions were then correlated to their antibacterial activity profile by means of Orthogonal Projection to Latent Sructure (OPLS) analysis. Peak area with retention time of 0.96 - 1.12 min present in chloroform fraction at UV 250 nm was found to significantly correlate to the antibacterial activity (Y related coefficient value 0.73).
THE INFLUENCE OF COATING MATERIAL DIFFERENCE AGAINST ENCAPSULATION EFFICIENCY OF RED GINGER OLEORESIN Jayanudin Jayanudin; Rochmadi Rochmadi; M. Kemal Renaldi; Pangihutan Pangihutan
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.881 KB) | DOI: 10.20961/alchemy.13.2.5406.274-286

Abstract

The purpose of this study was to determine the effect of different coating materials on the efficiency of red ginger oleoresin encapsulation and characterization of microcapsule surface. The encapsulation process begins by dissolving chitosan with 1 % (v/v) acetic acid and dissolving sodium alginate using aquadest to be used as a coating material. Red ginger oleoresin alginate included in the solution was stirred. The mixture entered into the chitosan solution and added tween 80 was stirred using a homogenizer to emulsify. The emulsion that forms included in the spray dryer to be converted into a powder of microcapsules, and then analyzes the encapsulation efficiency and surface morphology of microcapsules using scanning electron microscopy (SEM). Differences in the coating material used were chitosan, mixture of chitosan-alginate and mixture of chitosan-alginate-sodium tripolyphosphate (STPP). Weight ratio of red ginger oleoresin and the coating material were 1: 1, 1: 2 and 1: 3. The research results obtained were the highest encapsulation efficiency obtained from the coating material of the chitosan-alginate-sodium tripolyphosphate (STPP) at a ratio of 1: 3, namely by 85 %.
EXPLORATION OF BROTH CHICKEN GUT AS GROWTH MEDIA OF Escherichia coli BL21 pET-Endo FOR ENDO-Β-1,4-D-XYLANASE PRODUCTION Anak Agung Istri Ratnadewi; Moch. Yoris Alidion; Agung Budi Santoso; Ika Oktavianawatia
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.2.4356.191-204

Abstract

Endo-β-1,4-D-xylanase is a hydrolytic enzyme that breakdown the 1.4 chain of xylan polysaccharide. We have succes to transform the plasmid pET-Endo gene encoding endo-1,4-β-D-xylanase from Bacillus sp. originally from termites abdominal to E. coli BL21. The clone was ready for large scale of enzyme production. To reduce production cost, we look for subtitute media for the expensive Luria Berthani broth. Chicken guts broth is good alternative while rich of protein and very cheap. The content of N dissolved chicken guts broth reaches 87 % of LB broth. Growth of E. Coli BL21 in Chicken guts broth and LB broth (as control) was observed by Optical Density (OD) using spectrofotometer. Concentration of glucose added in broth and incubation temperature was varied. The result showed that optimal growth was in addition of 1.5 % glucose and incubated at  37 oC for 16 h. This optimal condition was used to grow E. coli BL21 pET-Endo for xylanase production. Enzyme purification was done by Ni-NTA affinity chromatography. Highest protein yield was 0.076 mg/mL obtained in 100 mM imidazole elucidation. The activity and specific activity of xylanase were estimated as 0.042 U/mL and 0.556 U/µg, respectively. The purification factor was 3.16 time and the molecular weight of enzyme was ± 30, 000 Dalton

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