cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
 15   16   17   18   19 
Front Cover AB Vol 11 No 1 (2007) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.453 KB) | DOI: 10.1234/123

Abstract

Editors Preface AB Vol 18 No 1 (2014) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/181

Abstract

Front Cover AB Vol 15 No 1 (2011) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.829 KB) | DOI: 10.1234/149

Abstract

Editorial Boards Vol 9 No 2 (2004) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.866 KB) | DOI: 10.1234/114

Abstract

Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece; D, Nurhayati; Farajallah, Ahmad; Anggraeni, A
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4236.897 KB) | DOI: 10.1234/59

Abstract

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene  on milk yield and percentage of milk protein  and  fat  in  local sheep. A total number of 83  heads of  lactating ewes  raised under an extensive management at  Jonggol Animal Science Teaching and Research Unit (JASTRU) of  the Faculty of Anima Science, Bogor Agricultural  University were  studied.  Research  activities  were  carried  out  through  some  step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P  R) then genotyped by single strand  conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on  the exon 7. The electrophoresis  pattern  revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for  those respective 5 type from the  highest to  the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%)  respectively. There were no  significant effect  polymorphism β-lactoglobulin gene on both milk yield and quality  in  I  cal  sheep at JASTRU.   Keywords : polymorphism. β-lactoglobulin in gene, PCR-SSCP and local sheep
Back Cover AB Vol 22 No 1 (2018) Rahmawati, Syamsidah
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.375 KB) | DOI: 10.14203/ab.v22i1.354

Abstract

Editorial Boards AB Vol 18 No 1 (2014) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.069 KB) | DOI: 10.1234/182

Abstract

Back Cover AB Vol 15 No 1 (2011) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/150

Abstract

Editors Preface AB Vol 14 No 1 (2010) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.612 KB) | DOI: 10.1234/141

Abstract

Preparation of An scFv-Based Immunoliposome Specific towards Transferrin Receptor Kusharyoto, Wien; Handayani, Ira; Sari, Martha; Fuad, Asrul Muhamad
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.3 KB) | DOI: 10.1234/99

Abstract

An ideal therapeutic for cancer would be one that selectively targets to tumor cells, is nontoxic to normal cells, and that could be systemically delivered, thereby reaching metastases as well as primary tumor. Immunoliposomes directed by monoclonal antibody or its fragments are promising vehicles for tumor targeted drug delivery. Transferrin receptors (TfR) levels are elevated in various types of cancer cells and considered to correlate with the aggressive or proliferative ability of tumor cells. Therefore, TfR levels can be elaborated as a prognostic tumor marker, and TfR is a potential target for drug delivery in the therapy of malignant cells. Here, we report the preparation of an anti-TfR single-chain antibody variable (scFv) immunoliposome for tumortargeted delivery vehicle. The cDNA encoding the variable heavy and light chain domains of the anti-TfRscFv antibody fragment was derived from the murine monoclonal antibody Clone E6, which is specific towards transferrin receptor. The gene encoding the anti-TfR scFv fragment was codon optimized for expression inEscherichia coli, subsequently synthesized, and cloned into the expression vector pJexpress404. The His6- tagged anti-TfR scFv fragment was expressed in E. coli and purified by means of immobilized metal-ion  affinity chromatography on TALON™ matrix. SDS-PAGE revealed that the scFv fragment had the size of approximately 27 kDa, which corresponded with the predicted size of the protein based on its amino acid sequence. Liposome containing 5% MPB-DOPE were prepared by ethanol injection method. Afterwards, the anti-TfR scFv fragments were covalently conjugated to the liposome to produce the anti-TfR scFv immunoliposome with the size of around 200 to 300 nm.

Page 17 of 54 | Total Record : 540

 15   16   17   18   19 

Filter by Year

2004 2021


Reset
Filter By Issues
All Issue Vol 25, No 1 (2021): ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses Vol 24, No 1 (2020): Annales Bogorienses Vol 23, No 2 (2019): Annales Bogorienses Vol 23, No 1 (2019): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses More Issue