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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Industria: Jurnal Teknologi dan Manajemen Agroindustri
Syamsidah Rahmawati, Syamsidah
Pusat Penelitian Bioteknologi-LIPI, Jl. Raya Bogor KM 46, Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Other

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Comparative Analysis of Rice Transformation Using Agrobacterium tumefaciens and Rhyzobium leguminosarum Rahmawati, Syamsidah; Jefferson, Osmat Azzam; Sopandie, Didy; ., Suharsono; Slamet-Loedin, Inez Hortense
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.394 KB)

Abstract

This study was aimed to study the effectiveness of Rhizobium transformation system compared to the most widely used Agrobacterium mediated transformation system on three rice cultivars, Ciherang (Indica), Nipponbare (Japonica), and Rojolele (Javanica). Six day old calli induced from immature embryos were inoculated with Rhizobium leguminosarum bv trifolii ANU845 and Agrobacterium tumefaciens LBA288 that harbored with vector pCAMBIA 5106. This plasmid contained a minimum set of transfer machinery genes and had a gusplus and an hptII gene driven by 35S CaMV promoter in the T-DNA. The results showed that the transformation frequencies (number of PCR positive plants per number of calli inoculated) ranging from 0 to 12.05 % depend on the genotype and transfer agent used. The highest transformation frequency (12.05%) was obtained in Ciherang transformed with R. leguminosarum. Most of the transgenic rice obtainedby Rhizobium transformation were normal in morphology and fertile similar to those obtained by Agrobacterium transformation. Integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis in T0 and T1 generations.Key words : Rhizobium leguminosarum, immature embryos, Agrobacterium tumefaciens
Konstruksi Vektor Biner Mengandung Gen Hibrid cryIB-cryIAa untuk Transformasi Agrobacterium Tanaman Padi (Oryza sativa L) Rahmawati, Syamsidah; Slamet-Loedin, Inez H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.725 KB) | DOI: 10.24002/biota.v9i2.2892

Abstract

This study aimed to obtain binary vectors carrying  cryIB-cryIAa hybrid gene suitable for Agrobacterium-mediated transformation of two Indonesian rice cultivars, Rajalele (Javanica) and Cisadane (Indica).  Introduction of hybrid Bt toxin, encoded by cryIB-cryIAa hybrid gene with different binding receptor in the insect gut driven by maize ubiquitin promoter, was intended to reduce the possibility of the appearance of stem borer resistant to Bt toxin. A HindIII fragment containing the ubi cryIB-cryIAa hybrid gene was excised from pBKS ubi cryIB-cryIAa and cloned into the HindIII site of binary vector pCAMBIA 1301, 1303, or 1304.  Restriction analysis of recombinant plasmids confirmed the insertion of the gene and demonstrated that the gene inserted in two orientations. The recombinant plasmids were transformed into electrocompetent Agrobacterium tumefaciens EHA105 by electroporation.  Embryogenic calli of Rajalele and Cisadane were co-cultivated with A. tumefaciens EHA 105 harbouring binary vector pCAMBIA 1301, 1303, or 1304 containing ubi cryIB-cryIAa. GUS histochemical assay revealed that high transformation efficiency (75-100%) on both cultivars were obtained from embryogenic calli transformed with pCAMBIA 1303 or 1304.
Consumer Preference Study on Myrtaceae Fruit Collection of Bogor Botanic Gardens Martiansyah, Irfan; Hariri, Muhammad Rifqi; Mulyani, Melza; Husaini, Iin Pertiwi A; Hidayat, Arief; Rahmawati, Syamsidah
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 10, No 1 (2021)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2021.010.01.5

Abstract

AbstractMyrtaceae fruit in Bogor Botanic Gardens (BBG) can be potentially developed as a unique and new fruit consumption style. The study aimed to determine the consumer preferences on Myrtaceae fruit. The method used was organoleptic testing, including a description, hedonic and rank tests. The parameters measured in this study are taste, color, flavor, texture, sound, and size of the fruit. Data analysis performed by the Kruskal-Wallis test. The results showed that most of the respondents preferred the fruit with a medium-size, tastes sour but not bitter, has attractive colors, has no pungent flavor, has soft and smooth texture, and is not crispy when it is bitten and chewed. The hedonic test showed that sample #4 and #7 have the highest average value of 4.7. The lowest average obtained in sample #3, where respondents did not like the sample. There was no significant difference of the average. The ranking of hedonic test result indicates the level of respondents' preferences in sequence are Eugenia sp. from Southeast Sulawesi, Myrtus pendula, Syzygium bankense, Syzygium aqueum, Eugenia sp. from Papua, Syzygium sp. from Seram Island, and Syzygium polycephaloides.Keywords: Bogor Botanic Gardens, consumer preference, Myrtaceae fruits, organoleptic test AbstrakBuah jambu-jambuan koleksi Kebun Raya Bogor berpotensi dijadikan buah konsumsi baru. Tujuan penelitian adalah untuk mengetahui dan menilai preferensi serta tingkat kesukaan masyarakat terhadap jenis buah jambu-jambuan koleksi Kebun Raya Bogor. Metode yang digunakan adalah uji organoleptik melalui metode uji deskriptif, uji kesukaan, dan uji rangking. Parameter uji meliputi rasa, warna, aroma, tekstur, suara, dan ukuran. Analisis data dilakukan secara deskriptif dan non-parametrik Kruskal-Wallis. Hasil penelitian memperlihatkan sebagian besar responden menyukai jenis buah berukuran sedang, rasa dominan masam tetapi tidak pahit, warna mencolok, aroma tidak menyengat, tekstur lunak dan halus, serta tidak renyah saat digigit. Hasil uji kesukaan menunjukan nilai rata-rata tertinggi sebesar 4,7 terdapat pada sampel #4 dan #7. Rata-rata terendah ditunjukkan pada sampel #3 sebesar 3,0, yaitu responden tidak menyukai sampel tersebut. Perbedaan rata-rata tersebut tidak signifikan. Perangkingan hasil uji kesukaan menunjukkan tingkat kesukaan responden secara berturut-turut adalah Eugenia sp. asal Sulawesi Tenggara, Myrtus pendula, Syzygium bankense, Syzygium aqueum, Eugenia sp. asal Papua, Syzygium sp, asal Pulau Seram, dan Syzygium polycephaloides.Kata kunci: buah jambu-jambuan, Kebun Raya Bogor, preferensi konsumen, uji organoleptik
A Comparative Study of the Organellar Genome of Gyrinops versteegii and Aquilaria malaccensis Hartati, Hartati; Cartealy, Imam Civi; Supatmi, Supatmi; Rahmawati, Syamsidah; Hartati, N Sri; Siregar, Ulfah Juniarti; Siregar, Iskandar Zulkarnaen
Jurnal Manajemen Hutan Tropika Vol. 30 No. 3 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.30.3.326

Abstract

Gyrinops versteegii and Aquilaria malaccensis are two important species of the Aquilarieae tribe. The main problem of this tribe is the challenge of species identification that is strongly dependent on the presence of flowers and fruit, which are not always available. The availability of whole genome information is expected to address the problems of species identification. This research aims to construct and compare the chloroplast and mitochondrial genomes of G. versteegii and A. malaccensis from short-read data using the NOVOplasty and GetOrganelle assembler. The chloroplast genome assembly revealed a full-length quadripartite circular structure with sizes of 174.814 bp (G. versteegii) and 174.821–174.822 bp (A. malaccensis), with highly conserved gene and organization. Meanwhile, the mitochondrial genome is multipartite with a size of 400.012 bp (G. versteegii) and 400.000 bp (A. malaccensis), with highly variable genes and organization due to the presence of gene cluster repeats. The LSC/IR/SCC region borders and phylogenetic analysis in chloroplasts indicate variations between the genomes of these two species. The investigation of nucleotide diversity in the chloroplast genome revealed that the trnL-rpl32 region had the highest nucleotide diversity (Pi = 0.03). This information will be useful in the future for a variety of downstream analyses.
Co-Authors Achmad Fariz Agus Rachmat Anky Zannati Apriadi Situmorang Cartealy, Imam Civi Chairunnisa Chairunnisa Didy Sopandie Dwi Astuti Dwi Widyajayantie Enung Sri Mulyaningsih Erdayani, Eva Estiati, Amy Estiati, Amy Hartati Hartati Hartati, N Sri Hery Widijanto Husaini, Iin Pertiwi A INEZ HORTENSE SLAMET-LOEDIN ISKANDAR ZULKARNAEN SIREGAR Kurniawan Rudi Trijatmiko, Kurniawan Rudi Loedin, Inez H.S MARTIANSYAH, Irfan Muhammad Rifqi Hariri Mulyani, Melza Nurhasanah, Ade Nena Osmat Azzam Jefferson, Osmat Azzam Pantouw, Carla F. S-Loedin, Inez H Satya Nugroho SLAMET-LOEDIN, INEZ H. Sri Indrayani, Sri Suharsono . Supatmi Supatmi TRI JOKO SANTOSO Ulfah Juniarti Siregar Vincentia Esti Windiastri, Vincentia Esti Yuli Sulistyowati