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ANNALES BOGORIENSES

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 05178452 EISSN : 24077518 DOI : -

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.

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Articles 540 Documents

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Guide for Authors AB Vol 19 No 2 (2015) puspita lisdiyanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Micropropagation of Tetramolopium klossii (Moore) Mattfeld, endemic subalpine and alpine plant species to New Guinea Tri Muji Ermayanti; Erwin Al Hafiizh; Andri Fadillah Martin; Ary Mandessy; Arief Hermono; Andi Mukhsia
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The protocol of micropropagation of Tetramolopium klossii (Moore) Mattfeld through adventitious shoot proliferation was established. This is the first report of T. klossii tissue culture. Seeds were surface-sterilized by dipping them in 10% of ethanol for 1 min followed by soaking in 1% of sodium hypochlorite for 10 min. The sterile seeds were germinated on MS medium solidified with 8 g/l of agar, supplemented with 20 g/l of sucrose. Two-month old seedlings formed some shoot clumps on MS solid medium without addition of any plant growth regulators. Small clumps containing 4-6 adventitious shoots were used for shoot proliferation on MS solid medium containing 0; 0.1; 0.5; 1.0 and 2.0 mg/L of BAP (benzylaminopurine) or kinetin. The results showed that medium MS without cytokinins (control treatment), and the addition of 0.1 or 0.5 mg/L of BAP was more effective for shoot proliferation compared to the medium with addition of BAP at higher concentration and the addition of kinetin. In further subcultures, multiple shoots grown on MS medium without cytokinins was more vigorous than that of shoots grown on other media, so that the shoots were ready for rooting. Medium containing 0.5 mg/L of BAP was the best for maintaining multiple shoot formation. Shoots formed roots on MS medium containing half strength of macro nutrients. Keywords: Tetramolopium klossii, micropropagation, Murashige and Skoog medium, shoot multiplication

Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity Sri Hartati; Marissa Angelina; Lia Meilawati; Indah Dwiatmi Dewijanti
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 mmol and blank of quercetin was 7.94 mmol and Vitamin C was 40.25 mmol. These compounds and antioxidant activities are the first time reported from this plant.

Denitrification of Activated Sludge in The Presence of Different Organic Substrates Dwi Agustiani; Takao Yamagishi
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The efect organic carbon on denitrifying activity was studied in batch reactor. For reactors were operated in paralel under anoxic condition in four different donor electrons. which were acetic acid (Reactor A) , methnol (Reactor M), phenol (Reactor P), and glucose (Reactor G) . The reactors. were fed with the artificial waste, which contain 721.8 mg/ NaNO3 The concentration or organic carbon added to the reactors were varied from TOD: N ratio of 0.5:1; 1:1, 1.5 : 1, to 2:1. The denitrification activity was e timated by measuring the reduction rate of nitrogenous oxide and N2O gas production. The denitrification capacity of adapted-sludge was also investigated, and the rates were estimated from the cumulative N2O (without acetylene inhibition) and N2 gas production . Reduction rate of nitrogenous oxide in all reactors increused during the investigation; the increase reduction rate were correlated to the increase of organic carbon concentration. The maximum reduction rate of nitrogenous oxide in reactor A was higher than those of the others. However, reduction rate in reactor M was more constant, so that nitrogenous oxides existed in th is reactor was removed faster. The highest potential denitrification rate (N20 production) was observed in sludge of reactor . However, N2 gas recovery from nitrate and nitrite transformed by sludge of reactor M was the highest. Linear correlation between nitrogenous oxide reduction with gas production was observed in reactor A, M and P, but not in reactor G.

Guide for Authors AB Vol 10 No 1 (2005) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria Ahmad Thontowi; Elvi Yetti; Yopi Yopi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Alkanes are major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely a-proteobacteria (57 %), g-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). g-Proteobacteria and a-proteobacteria which seems to play an important role in the alkane biodegradation.

Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach N Sri Hartati; Supatmi Supatmi; Pramesti Dwi Aryaningrum; Enny Sudarmonowati
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Cassava is an important carbohydrate source that provides food security and alternative renewable energy development. This plant is naturally drought tolerant, but there is a wide variation within cassava genotypes in their ability to maintain high yield and starch production under drought stress. It has been reported that cassava clones with leaf retention or stay green trait can produce more total fresh biomass and high root dry matter compared to drought susceptible cultivars. The genetic, biochemical and molecular bases of stay green trait need to be understood to develop drought resistant cassava cultivars since water stress limits yield and starch production. Differential Display (DD)RT-PCR is a powerful technique for analyzing differences in gene expression. The method is based on the detection of the differentially expressed cDNAs from two or more samples. The main objective of this study was to identify differentially expressed cDNA in cassava under drought stress by employing a cDNA-RAPD approach. In this study, we used cassava genotype Ubi Kuning which was considered the most responsive to water insufficiency (45 days without watering). Leaf samples were collected from water stressed and well-watered plants at day 45. Among 11 random primers, OPB03 and OPH17 have identified differentially expressed cDNA in Ubi Kuning. Further characterization of these PCR products of expressed cDNA under drought stress may open possibility the development of cassava with improved drought resistance through genetic engineering and/or marker assisted selection (MAS).

Editorial Boards AB Vol 20 No 2 (2016) Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Control of Biodegradability of Polyurethane Foam Based on Palm Oil by Ratio of Soft Segment on the Polymer Backbone Agus Haryono; Evi Triwulandari; Dewi Sondari; Ahmad Randy; Asep Muhamad Ridwanuloh
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Polyurethane is polymer synthetic which is formed by reaction between polyols and polyisocyanates.polyols are compounds which contain two or more hydroxyl groups. Polyurethane can be applied in various forms, such as foam, elastomeric, coating and adhesive. In this work, we developed pol yurethane foams by using polyols based on palm oil. Palm oil based polyols as soft segments in the polyurethane backbone accelerate biodegradable process of the polyurethane. Biodegradability is the ability of material to be utilized as a carbon source by microorganisms and converted safely into carbon dioxide, biomass and water. Commercially available polyurethane foams are usually produced from petrochemical based polyols that they are not biodegradable. The biodegradability study was conducted by using Aspergillus niger (fungi) and Pseudomonas aeruginosa(bacteria), with variation of method and media for biodegradation. Rigid polyurethane foam was synthesized by using one shoot process method, which is reaction between palm based polyol mixture and methylene diphenly diisocyanate (MDI) at room temperature. The effect of variation of ratio of polyisocyanates (NCO) as hard segment and polyols (OH) as soft segment to the properties of polyurethane foams were studied in detail. Study of the effect NCO/OH ratio was conducted with variation ratio of 0.8, 1.0 and 1.2. Increasing hard segment (NCO) ratio to the soft segment (OH) affected in the decreasing of hydrophilicity of foam. Thus,the penetration of water into bulk of polymer decreased. The biodegradability of foam was also affected by the soft segment ratio on the polymer backbone. Keywords: biodegradable, polyurethane, palm oil, polyols, soft segment

Guide for Authors AB Vol 18 No 2 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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