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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis Bugi Ratno Budiarto; Azamris Azamris; Desriani Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.051 KB) | DOI: 10.14203/ann.bogor.2017.v21.n2.52-62

Abstract

Reliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique is hampered by a false positive result. In attempt to develop a  TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR without enzyme treatment using recombinant plasmids that contained HER2I655V gene with represented its alleles was used to evaluate the presence of false positive  result while DNase I treated-PCR reagent was used in TARMS-PCR to evaluate the effective dose of the enzyme and further to adjust the TARMS-PCR conditions.  PCR master mix kit used in this study produced a false positive result on HER2I655V TARMS-PCR as proven by the presence of multiple PCR products in Non-Template Control (NTC) and 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allele-specific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template
In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Maria Imelda; Aida Wulansari; Sumarnie Sumarnie
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2552.626 KB) | DOI: 10.14203/ann.bogor.2008.v12.n1.39-43

Abstract

Pandanus  conoideus Lam  or  'Buah  merah'  of the  Pandanaceae  is  native  to  East  Indonesia,  particularly Papua and  North Maluku. Traditionally  the  fruits  are  used  for health  promotion and maintenance as well  as  for curing  several  illnesses.  Recently,  it  has  been  reported  that  the  fruits  are  potential  for  cancer medication . As a result, there  has  been  an  overexploitation of the  plants  from  their habitats .  In  order  to  anticipate  their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation and  cultivation  of these plants should be developed. Generally  buah merah is propagated vegetatively by  offshoots and stem cuttings or generatively by seeds.  Micropropagation  has  many  advantages  over  the conventional method. because  the  technique allows mass  cIonal  and  pathogen-free  production of plants  at high  rate of multiplication all  year round . In  this  research the  effects of 0,1 -0.2 mg/l thidiazuron  (TDZ), 0.5- 1.0 benzyl  amino  purine  (BAP)  and  0.25-0.5  mg/l  Kinetin  (KN)  on  shoot  bud  induction  and  proliferation of P.  conoideus were  investigated  using  nodal   sections  or  lateral buds  or P.  conoideus  on modified  Murashige and skoog medium. Shoots were rooted on MS medium  without plant growth  regulators (PGR) . The  result  showed that  lateral  buds  of Pandanus  started  to  initiate  growth after 4-7  days  in  culture.  The  best medium  for  shoot proliferation  was MS  containing either 0,5 mg/l  BAP with  0.1 mg/ l  TDZ or  I  mg/l  BAP with  0.5  mg/I  KN. giving a multiplication rate of  I6.5  shootlets per shootbud explant after 8 weeks Rooting of shoots was successfully conducted on MS medium without PGR.  Acclimatization of  rooted plantlets was achieved on  a mixed medium of  cocopeat and soil (1:1). Keywords: Buah merah (Pandanus conoideus). lateral buds, BAP. TDZ. KN
Expression of No Affinity Tagged Recombinant Human Interferon Alpha-2a in Methilotropic Yeast Pichia pastoris Neng Herawati; Andri Wardiana; Ratih Asmana Ningrum
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2015.v19.n2.57-62

Abstract

Recombinant human interferon alpha-2a (rhIFNα-2a) has been widely used for clinical therapy as antiviral, anticancer as well as immunomodulator. In this study, the open reading frame (ORF) encoding synthetic hIFNα-2a was constructed to be in framed with N-terminal alpha factor secretion system in methylotropic yeast Pichia pastoris. This research aimed to construct, express and analyse the non-affinity tagged recombinant human interferon alpha-2a in the methilotropic yeast P. pastoris. We used pPICZαB plasmid for cloning and expression vector. The confirmed recombinant plasmid containing the correct DNA sequence of hIFNα-2a was linearized by SacI restriction enzyme, then transformed into P. pastoris genome using electroporation. We screened two multi-copy recombinants in YPDS plates containing Zeocin™. Buffered complex medium containing 0.5 % methanol (BMMY) was used for protein expression for 48 hours in the culture condition. The recombinant protein was purified by blue sepharose affinity chromatography. Analyses of hIFNα-2a protein by SDS-PAGE and Western blot confirmed that protein band in which was observed around 19.2 kDa, was recombinant hIFNα-2a. The quantification of purified rhIFNα-2a using colorimetric binichoninic assay (BCA) informed that the yield was 44 mg/L culture (OD600= 2-3).
Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Satya Nugroho; Vincentia Esti Windiastri; Dwi Widyajayanti; Carla Frieda Pantouw
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.796 KB) | DOI: 10.14203/ann.bogor.2009.v13.n1.29-36

Abstract

Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority.  The screening protocol based on PEG 6000 has been developed and was  being used to screen 70 mutant lines to characterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds).  These characters were used to score the Degradation Index  and Vigour Index. Results showed varrying responds of the lines to the osmotic presure.  Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified.  Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.   Keywords:  Oryza sativa, insertion mutant, drought, PEG 6000, Degradation Index, Vigor Index.
Guide for Authors AB Vol 18 No 1 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.545 KB) | DOI: 10.14203/ann.bogor.2014.v18.n1.%p

Abstract

Editor's Preface AB Vol 15 No 1 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.564 KB) | DOI: 10.14203/ann.bogor.2011.v15.n1.%p

Abstract

Editorial Boards AB Vol 23 No 1 (2019) ario tutuko
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (20.371 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.%p

Abstract

Guide for Authors Vol 9 No 2 (2004) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.84 KB) | DOI: 10.14203/ann.bogor.2004.v9.n2.%p

Abstract

Editorial Boards AB Vol 19 No 1 (2015) Muhamad Dzikri
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.713 KB) | DOI: 10.14203/ann.bogor.2015.v19.n1.%p

Abstract

Motif Ii of Japanese Encephalitis Vius Ns3 Pro is Not Essential for Rna Binding Activit Andi Utama; Hiroyuki Shimizu
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2930.744 KB) | DOI: 10.14203/ann.bogor.2007.v11.n1.15-21

Abstract

The role of motif n  (DEAR AsPw-GlulK,,-Ala.,.7-Hi  1~~  )  of Japanese encephalitis  virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to  the motif and  the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL2l(DE3) pLysS and purified by metal affinity resin. Asp-285 - and Glu-286 was respectively substituted with Ala, AJa-287 was replaced by Cys.,Gly.  or ser. His-288 was mutated to other 19 amino acids. In total. 24 mutant proteins were produced and analyzed. AL results, all mutants showed quite similar RNA binding activity.  Indicating that motif II of JEY NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C virus c (Hey) NS3 protein, suggesting the similar structure of NS3 protein in flavivirus .Keywords: Japanese encephalitis, R.binding, motif

Page 44 of 54 | Total Record : 540

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