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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Editor's Preface AB Vol 15 No 2 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.569 KB) | DOI: 10.14203/ann.bogor.2011.v15.n2.%p

Abstract

Potency of Curcuma aeruginosa as A Chemopreventive Candidate Agent on 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Rat Spleen Asri Sulfianti; Nur Hasanah; Agung Eru Wibowo; Kurnia Agustini; I Made Artika
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.676 KB) | DOI: 10.14203/ann.bogor.2019.v23.n2.58-65

Abstract

Present investigation shows that the extract of C. aeruginosa attenuates DMBA-induced spleen carcinogenesis in Wistar rats. Three-week female Wistar rats were treated with three different C. aeruginosa extract doses (CA1: 40 mg/200 g body weight, BW; CA2: 80 mg/200 g BW; CA3: 160 mg/200 g BW) and were induced with DMBA after one-week administration of these doses. A commercial immunostimulant, and DMBA only were also given to each group as positive and negative control, respectively. The development of tumors was evaluated by investigating the incidence of tumor and tumor multiplicity during the experiment. Spleen mass index and histological parameters such as white pulp, centrum germinativum, and marginalis zone were also examined. Based on our study, the administration of C. aeruginosa extract during and after carcinogen induction gave several impacts on rat carcinogenesis. At the extract dose of 80 mg/200 g BW, tumor incidence of animals were least (P<0.05). However, all doses did not show any effect to the spleen mass index, though the highest dose (160 mg/200 g BW) was found to cause changes in white pulp and marginalis zone boards. This trend indicates that it takes higher dose to cause an immune response effect reaching the organs.
The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Anggia Prasetyoputri; Neti Yuliaty; Warda Tuharea; Alisin Febyanti Febyanti; Bambang Sunarko; Ines I.C Atmosukarto
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.809 KB) | DOI: 10.14203/ann.bogor.2010.v14.n2.9-14

Abstract

Emerging resistant viral  strains combined with the  limited availability of antivirals  in  a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus  -  a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli  cells.  Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD  at  600nm  (OD) of  0.4-0.6.  Once  at  the  required  OD,  the  culture  was  split  in  two  aliquots  and expression  of  the  M2  protein  was  induced  in  one  of  the  duplicates  with  the  addition  of  isopropyl  β-Dthiogalactopyranoside (IPTG). Bacterial growth was monitored at 60 -minute intervals. Exogenous expression of  the  M2  protein  has  been  reported to  decrease  host  cells  viability,  resulting  in  lower  OD 600 values. Our results  suggest  that  the  M2  protein  was  expressed  and  that  overexpression  of  this  protein  resulted  in consistently lower OD 600  values of induced cultures compared with that of uninduced cultures. Based on this principle,  extracts  can  be  screened  for  their  ability  to  block  M2  function  as  identified  by  increased  OD 600 values.   Keywords: influenza, M2, bioassay, gene synthesis, antiviral
Antioxidant Capacities of Holothuria Sea Cucumbers Tutik Murniasih; Masteria Yunovilsa Putra; Ratih Pangestuti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.351 KB) | DOI: 10.14203/ann.bogor.2015.v19.n2.21-26

Abstract

Sea cucumbers are a potential source of bioactive metabolites for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activityof several extracts of sea cucumbers, Holothuria scabra, Holothuria atra, Holothuria leocospilota, and Holothuria excellens collected from Lombok Island. Phytochemical screening revealed the presence flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH model were evaluated. The crude extract of H. leocospilota and H. atra showed the highest potential antioxidant capacity. H. atra extract was separated by column chromatography silica gel eluting with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. However, these are preliminary findings and further studies will have to focus on the isolation of bioactive substances derived from H atra and identify other health benefit effects of H. atra.
Expression of an Anti-Transferrin Receptor Antibody SCFV Fragment in Escherichia coli Using A L-Rhamnose-Based Tightly Regulated Promoter System Dian Andriani; Ira Handayani; Wien Kusharyoto
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3611.759 KB) | DOI: 10.14203/ann.bogor.2012.v16.n2.1-6

Abstract

Transferrin receptor (TfR) levels are elevated in various types of cancer cells and correlate with the aggressive or proliferative ability of tumor cells. Therefore, TfR levels are considered useful as a prognostic tumor marker, and TfR is a potential target for drug delivery in therapy of malignant cells. In such kind of targeted delivery system, antibody fragments are frequently used as targeting moiety. Here, we report the generation of an anti-TfR single-chain antibody variable (scFv). The cDNA encoding the variable of heavy and light chain domains of the scFv antibody fragment was derived from the anti-TfR monoclonal antibody of LUCA31. The gene encoding the anti-TfR scFv fragment was codon, optimized for expression in Escherichia coli, subsequently synthesized, and cloned into the pJExpress-804 Rhamex vector. The expression vector utilizes the E. colirhaB promoter and corresponds to the regulatory genes, and is tightly regulated by the presence of L-L-rhamnose. It is also tightly regulated in the absence of L-L-rhamnose by the addition of D-glucose The His6-tagged anti-TfR scFv fragment was expressed in E. coliNiCo21 and purified by means of immobilized metal chelate affinity chromatography on TALON™ matrix. In SDS-PAGE, a single band corresponding to a molecular mass of approximately 30 kDa was observed whether it corresponded to the predicted molecular mass based on the amino acid sequence.Keywords: antibody fragment, L-L-rhamnose, Rhamex vector, scFv, transferrin receptor
Front Cover AB Vol 18 No 1 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2014.v18.n1.%p

Abstract

Editor's Preface AB Vol 14 No 2 (2010) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.57 KB) | DOI: 10.14203/ann.bogor.2010.v14.n2.%p

Abstract

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Syamsidah Rahmawati; Chairunnisa Chairunnisa; Eva Erdayani; Satya Nugroho; Amy Estiati
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.857 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.30-40

Abstract

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains  also increased significantly (~16-22%).  After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress.  Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.
Guide for Authors AB Vol 9 No 1 (2004) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 9, No 1 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (858.727 KB) | DOI: 10.14203/ann.bogor.2004.v9.n1.%p

Abstract

Editor's Preface AB Vol 21 No 2 (2017) Syamsidah Rahmawati
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.244 KB) | DOI: 10.14203/ann.bogor.2017.v21.n2.%p

Abstract

Page 47 of 54 | Total Record : 540

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