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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Back Cover AB Vol 19 No 2 (2015) lisdiyanti, puspita
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.283

Abstract

In-Silico Cloning and Analysis of Divalent Subunit OMP31-SODc Proteins As A Prophylaxis Vaccine Against Brucella melitensis Infection Wijaya, Sri Kartika; Kusumawati, Arizah; Wardiana, Andri; Rubiana, Yana; Husnaa, Ulfatul; Santoso, Adi
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.993 KB) | DOI: 10.14203/ab.v19i1.94

Abstract

The urgency to develop a new protein based subunit vaccine candidate against Brucella was provoked by its frequent infection to human and lives stock. Since Brucella melitensis is found as the most species isolated from human, thus the outer membrane of the Brucella melitensis become prominent subcellular location for searching promising antigen to be developed as vaccine target due to its interaction with cell host. Among other proteins suggested by Vaxign program, the OMP31 is found as a promising candidate. Moreover, analysis on other subcellular location leads our interest to SODc protein, which is expected to support the OMP31 in triggering immune response. The OMP31-SODc divalent vaccine candidate was analysed in-silico to predict its stable three-dimensional structure, cloning process and expectation on the ease during expression, purification and the protein vaccine delivery to develop expected immune response.
An Application of Reverse Transcriptase Polymerase Chain Reaction in A Relative Quantification of Gene Expression Santoso, Adi
ANNALES BOGORIENSES Vol 9, No 1 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/4

Abstract

The ability to quantify steady state levels of individual mes enger-RNA (mRNA) transcripts has been the key issue for study on the control of gene expression. Although two available techniques, Northern blot and nuclease protection assays (NI)A) have been widely used tor detecting mRNA, these techniques have critical limitations. The most obvious limitation of these two techn iques is the required number of target mRNAs to be detected. Reverse transcription-polymerase chain reaction (RT-P R), which has been accepted as a highly sensitive and specific method, provides a means for detecting and quantifying gene expression using, theoretically only a single molecule of mRNA. The ensitivity and reliability ofRT-PCR i dependent upon both the RT and PCR steps. The PCR step ha been problematic because of the exponential nature of this reaction where small variation can lead Lo dramatic changes in final result. here fore, the use of RT-P R for quanti fication of gene expres ion requires pre-experimental planntng and de ign. In thiS experiment, the procedure fOT pre-experimental planning, linear range determination and subsequent relative quantification of gene expression are described in detail. study of ornithine decarboxyJase gene, a gene involved in the polyamine biosynthesis and temporally expressed, dunng embryogenesis of Musca domestica (housefly) was used as the model. The re ults show that during early embryogenesis (t-l to t-4) the expression lev I was very low. The increase In expression profile was observed started at t-5, peaked at t-9, and followed by substantial decrease from t-l 0 to t- 12.  
The Emergence of Biosimilars in Indonesia: Guidelines, Challenges and Prospects Wardiana, Andri; Ningrum, Ratih Asmana
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.272

Abstract

According to the Food and Drug Administration (FDA), biosimilar is defined as a product which is highly similar to the reference product without clinically meaningful differences in safety, purity and potency. Indonesia is a developing country which has more than 250 million people. The domestic need of Biosmilar in Indonesia is about 10-20%.  Even though the need is very high, Indonesia still has not been able to produce Biosimilar independently. To stimulate the domestic production on biosimilar, National Agency for Drug and Food Control (NADFC) Republic of Indonesia has assigned Regulation of Biosimilar as Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 17 Tahun 2015 Tentang Pedoman Penilaian Produk Biosimilar. The guidance covers the quality requirement and evaluation of Biosimilar products. The Ministry of Health of Indonesia has a strategic plan in biopharmaceutical covering biosimilar which is going to develop in 2015-2025. The strategy is expected to initiate biosimilar production in Indonesia. This review focuses on the guidelines, challenges and prospects biosimilars in Indonesia comparing to other international regulatory bodies.Keywords: Biosimilar, Indonesia, Guidelines, Challenges and Prospects
Editors Preface AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1324.165 KB) | DOI: 10.1234/171

Abstract

Front Cover AB Vol 21 No 2 (2017) Rahmawati, Syamsidah
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v21i2.317

Abstract

Back Cover AB Vol 19 No 1 (2015) Dzikri, Muhamad
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.269

Abstract

Expression of An Immunogenic Intimin Fragment of EHEC O157:H7 in Escherichia coli Periplasm under The Control of A Rhamnose-Based Regulated Promoter Hariyatun, Hariyatun; Suwanto, Antonius; Kusharyoto, Wien
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/90

Abstract

Intimin is the main adhesin of Enterohemorrhagic E. coli (EHEC) O157:H7 bacteria which are the most common leading infectious cause of bloody diarrhea and acute kidney failure in children who develop hemolytic uremic syndrome (HUS). Intimin is required for persistent bacterial colonization to eukaryotic host cell and its receptor-binding activity is localized at the C-terminus 282 amino acids (Intimin282). Thus, Intimin282 is an attractive antigen candidate that could be useful in vaccine and diagnostic systems against EHEC infections. Previous studies had reported expression of Intimin in E. coli cytoplasm using commonly used prokaryotic expression systems. However, it usually encountered several problems, i.e. low expression level, leaky expression, inclusion body formation, and truncated protein. The pRHA vector, which is tightly regulated by Lrhamnose and D-glucose, represents a viable alternative E. coli expression system to overcome such problems. Moreover, E. coli periplasm has an advantage of maintaining protein functionality by providing an oxidative environment that is more efficient than cytoplasm. However, to date there is no study about Intimin expression using pRHA expression system and/or in E. coli periplasm. Accordingly, we constructed a recombinant pRHA vector harbouring the respective gene to investigate the expression of an immunogenic Intimin fragment of EHEC O157:H7 in E. coli periplasm. The gene encoding His6-tagged Intimin282 (Int282) together with pelB signal sequence was cloned into the pRHA vector, subsequently expressed in E. coli JM109 and purified. Expression and purification of Int282 were verified by SDS-PAGE and Western blot. The result showed that Int282 was successfully expressed in E. coli periplasm with a protein size of approximately 32 kDa, which corresponded with the predicted size of the protein based on its amino acid sequence.
Protective Effect of CD4+CD25+ Regulatory T Cells on Mice Model of Rheumatoid Arthritis Rifai, Muhaimin
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.709 KB) | DOI: 10.1234/30

Abstract

Naturally occurring CD4+CD25+ regulatory T (Treg) cells, a component of the innate immune response which play a key role in the maintenance of self-tolerance, have become the focus of numerous studies over the last decade. These cells have the potential to be exploited to treat autoimmune disease. These cells inhibit the immune response in an antigen-nonspecific manner by interacting with other T cells. These T cell populations actively control the properties of other immune cells by suppressing their functional activity to prevent autoimmunity but also influence the immune response to allergens as well as against tumor cells and pathogens. In this experiment we showed that induced regulatory T cells have a protective effect on mice model of rheumatoid arthritis (RA). RA mice which were injected intraperitoneally with Andrographis paniculata substrate or injected with induced regulatory T cells showed the effects of recovery. We further showed that the generation of leukocyte including B cells can be promoted by the administration of A. paniculata substrate. Tissue damage from free radicals that arise due to imperfect metabolism can be prevented by such treatment in RA model mice. Recovery effects occurred in RA model mice involves the increasing number of CD4 +CD25+ regulatory T cells.Keywords: CD4+CD25+, Regulatory T cells, Rheumatoid Arthritis, Andrographis paniculata, Autoimmune
Front Cover AB Vol 20 No 1 (2016) Anugerah, Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.598 KB) | DOI: 10.14203/ab.v20i1.260

Abstract

Page 5 of 54 | Total Record : 540

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