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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata Artanti, Nina; Mcfarlane, Ian James
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.798 KB) | DOI: 10.1234/39

Abstract

Asparagine synthetase (AS) is one of the enzyme involves  in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we  investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate...cDNAs enconding  Asparagine synthetase  from  C. rostrata because of the asparagine accumulate in this culture.  Several PCR based approaches  were conducted such as RT-PCR, LM-PCR and RACE. Using these methods, two AS cDNAs was  isolated from C. rostrata. AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence.  (GenBank no AF488726). Thesetwo cDNAs had high homology with the legume AS. Keywords: cDNA, nitrogen metabolism, asparagine synthetase, Coronilla rostrata
Guide for Authors AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.473 KB) | DOI: 10.14203/ab.v21i1.306

Abstract

Front Cover AB Vol 19 No 1 (2015) Dzikri, Muhamad
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.265

Abstract

Insect Bioassay in Biosafety Containment to Select Transgenic Rice (Oryza sativa L.) Harboring Cry1B Gene Resistant to Yellow Stem Borer (Scrirpophaga incertulas Walk.) Estiati, Amy; Nena, Ade; Nugroho, Satya
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/77

Abstract

Development of rice varieties resistant to yellow stem borer (YSB) is very crucial.  Agrobacterium-mediated transformation of Cry1B gene under wound inducible gene promoter mpi (maize proteinase inhibitor) into a local rice variety Rojolele had been conducted. PCR analysis proved that Cry1B gene had been integrated into plant genome of 3R25 and 3R5 rice lines. Segregation analysis using PCR for Cry1B gene of the two putative transgenic rice lines at third (T2), fourth (T3), fifth (T4) and sixth (T5) generations of 3R25 and 3R5 lines proved that 3R25.7.27, 3R25.7.13.8.2, 3R25.7.13.8.6, 3R25.7.13.8.8, 3R5.26.2 and 3R5.26.5 are homozygous lines for Cry1B. Insect bioassay on three randomly picked homozygous transgenic rice lines to study the efficacy of Cry1B gene toward YSB was conducted in biosafety containment by infestingYSB larvae at first instar into 3R25.7.27, 3R25.7.13.8.6 and 3R5.26.5 transgenic rice lines, using non-transgenic Rojolele, IR64 and IR74 as susceptible controls. The results showed that the precentages of deadhearts symptoms of  3R25.7.27, 3R5.26.2 and 3R25.7.13.8.6 rice lines were lower than those of the susceptible control lines with scores of 0,1 and 0, respectively. While the scores of all three susceptible control plants were 9. The results proved that lines 3R25.7.27, 3R25.7.13.8.6 and 3R5.26.2 were categorized as resistant lines while the non-transgenic Rojolele, IR64 and IR74 were categorized as susceptible lines. The results also showed that the Cry1B gene was expressed and  produced insecticidal protein CRY1B which were active against YSB to protect rice plant toward YSB infestation
Effect of culture conditions on phytase production by Aspergillus ficuum in solid-state fermention using rice bran as substrate Kusharyoto, Wien; Sari, Martha; Ridwanuloh, Asep Muhammad
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.142 KB) | DOI: 10.1234/23

Abstract

Phytic acid is an antinutritional factor that forms 1–2% of most of the seeds and their co-products representing more than 60%  of their total phosphorus. Monogastric and agastric animals are unable to utilize phytate phosphorus either due to lack of or insufficient amount of phytate degrading enzymes. Phytases (myo-inositol hexakisphosphate-phosphohydrolase) are a special class of phosphatases that catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol and inorganic phosphate. Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been purified and characterized from various fungi, yeasts and bacteria. The present investigation involves studies on the effect of moisture content, pH value and different media ingredients such as carbon, nitrogen, and surfactants on the production of phytase by the fungus  Aspergillus ficuum DSM 932 in solid-state fermentation (SSF) using rice bran as substrate. The production of phytase by SSF was favored, when the fungus was grown at a moisture content of 60% and pH 7.0, resulted in a phytase activity of 5.2 units/g dry substrate. There was a 20% increase in phytase yield in the presence of sucrose in SSF medium, while glucose and fructose were not effective in enhancing the phytase activity when used individually. Yeast extract was found to be a favorable nitrogen source for phytase production by SSF, which resulted in a 20% increase in phytase activity. There was no significant effect in increasing phytase production with the use of either soy peptone or tryptic  soy as nitrogen source. Approximately 30% inhibition in phytase activity was shown in the presence of the surfactant Tween-80 or Triton X-100 in the SSF. By supplementing rice bran with sucrose and yeast extract, and performing the SSF in tray bioreactors, a phytase activity of 6.76 units/g dry substrate could be obtained.Keywords:  phytase, solid-state fermentation, Aspergillus ficuum, nutritional factors, rice bran
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hani; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.451 KB) | DOI: 10.14203/ab.v20i1.234

Abstract

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.
In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Imelda, Maria; Wulansari, Aida; Sumarnie, Sumarnie
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2552.626 KB) | DOI: 10.1234/61

Abstract

Pandanus  conoideus Lam  or  Buah  merah  of the  Pandanaceae  is  native  to  East  Indonesia,  particularly Papua and  North Maluku. Traditionally  the  fruits  are  used  for health  promotion and maintenance as well  as  for curing  several  illnesses.  Recently,  it  has  been  reported  that  the  fruits  are  potential  for  cancer medication . As a result, there  has  been  an  overexploitation of the  plants  from  their habitats .  In  order  to  anticipate  their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation and  cultivation  of these plants should be developed. Generally  buah merah is propagated vegetatively by  offshoots and stem cuttings or generatively by seeds.  Micropropagation  has  many  advantages  over  the conventional method. because  the  technique allows mass  cIonal  and  pathogen-free  production of plants  at high  rate of multiplication all  year round . In  this  research the  effects of 0,1 -0.2 mg/l thidiazuron  (TDZ), 0.5- 1.0 benzyl  amino  purine  (BAP)  and  0.25-0.5  mg/l  Kinetin  (KN)  on  shoot  bud  induction  and  proliferation of P.  conoideus were  investigated  using  nodal   sections  or  lateral buds  or P.  conoideus  on modified  Murashige and skoog medium. Shoots were rooted on MS medium  without plant growth  regulators (PGR) . The  result  showed that  lateral  buds  of Pandanus  started  to  initiate  growth after 4-7  days  in  culture.  The  best medium  for  shoot proliferation  was MS  containing either 0,5 mg/l  BAP with  0.1 mg/ l  TDZ or  I  mg/l  BAP with  0.5  mg/I  KN. giving a multiplication rate of  I6.5  shootlets per shootbud explant after 8 weeks Rooting of shoots was successfully conducted on MS medium without PGR.  Acclimatization of  rooted plantlets was achieved on  a mixed medium of  cocopeat and soil (1:1). Keywords: Buah merah (Pandanus conoideus). lateral buds, BAP. TDZ. KN
Identification Of Degradation Pathway Of Vinylacetate Using Bacterial Isolate V2 And Characterization Of The Involved Enzymes Sunarko, Bambang; Sulistinah, Nunik; Nieder, Maria; Meyer, Ortwin
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/13

Abstract

Vinyl acetate is a toxic substance, but has a high commercial value. In this study we show that vinyl acetate is subject to microbial degradation at rates of up to 6.38 and 1 mmol/h per g (dry weight) under aerobic and anaerobic conditions, respectively. It was hydrolyzed by bacterium V2 to ethano, acetaldehyde and acetate. The enzymes involved in the metabolism of vinylacetate were vinyl acetate esterase, aldehyde dehydrogenase, and alcohol dehydrogenase, which localized in the cytoplasmic fraction. The Km values of vinyl acetate esterase and alcohol dehydrogenase were 6.13 mM and 0.24 mM. respectively. Vinyl acetate esterase hydrolyzed the ester to acetate and vinyl alcohol. The Latter isomerized spontaneously to acetaldehyde and was then converted to acetate.The acetaldehyde was disproportionated into ethanol and acetate. The acetate was then converted to acetyl coenzyme A and oxidized through the tricarboxylic acid cycle and the glyoxylate bypass .  
Editorial Boards AB Vol 19 No 2 (2015) lisdiyanti, puspita
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.607 KB) | DOI: 10.14203/ab.v19i2.286

Abstract

The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Chrismadha, Tjandara; Sartika, Diani; Setyaningsih, Iriani; Uju, Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/47

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 

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