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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Development of Somatic Embryo in Lithospermum erythrorhizon Siebb. et Zucc and The Study on The Effect of Methyl Jasmonate on its Maturation Totik Sri Mariani; Octavia Ramayanti; Kazufumi Yazaki; Hiroshi Miyake
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3351.663 KB) | DOI: 10.14203/ann.bogor.2004.v9.n2.72-79

Abstract

A research on the somatic embryogenesis in Lithospermum erythrorhizol1 has been conducted. Embryogenic callus was inoculated in EIM9 liquid medium (modification ofLS medium), i.e. L2PVP (initiation medium) and the development of somatic embryo was observed. Ten J.1M and 100).lM methyl jasmonate was added into L3PVP medium (differentiation medium) for somatic emb yo maLUration. The purposes of this research were to observe tlle development of somatic embryo and to observe the effect of methyl jasmonate on maturation of the somatic embryo in L erylhrorhizol/ . The results showed that somatic embryogenesis in L erythrorhizon derived from single cells differentiated further forming proembryo, globular. heart, torpedo and cotyledon stage. Treatment with 10 f.1M and 100).lM methyl jasmonate induced maturation of somatic embryos (cotyledon stage) after transferring them to embryo development medium (L4PVP).
Back Cover AB Vol 24 No 2 (2020) ario tutuko
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.%p

Abstract

Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara Indriati Ramadhani; Hasnadiazahra Rohadi; Yeni Yuliani; Muhammad Ilyas
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.66-73

Abstract

An assemblage of endophytic fungi was isolated from Moringa oleifera Lam. collected from Lombok island, West Nusa Tenggara Province, Indonesia. Fungal endophytes were isolated using surface sterilization methods with slight modification. Forty-six selected endophytic fungal strains were isolated from the leaves, petioles, and stems of M. oleifera. The fungal strains identification through morphological observation and ITS rDNA-based molecular analysis showed that fungal endophytes were associated with host plants belonging to the taxa Alternaria, Cladosporium, Colletotrichum (Glomerella), Corynespora, Curvularia (Cochliobolus), Fusarium, Mucor, Ochrocladosporium, Phomopsis (Diaporthe), and Trametes. In this study, endophytic Phomopsis dominating the obtained strains, whereas 26% (12/46) strains were isolated from host plant samples.
Overexpression of Sugarcane Sucrose Transporter 1 (SoSUT1) Gene Increases Rice Yield Cesha Ananda Putri; Bambang Soegiharto; Parawita Dewanti
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.18-26

Abstract

The growth and development of plants are determined by photosynthesis, which ultimately results in sucrose. Sucrose is synthesized in the source then translocated to all parts of the plant (sink). The translocation process of sucrose from source to sink is controlled by sucrose protein called sucrose transporter. SoSUT1 is a gene that encodes a sucrose transporter 1 (SUT1) protein in sugarcane. Rice transformation with the SoSUT1 causes overexpression SUT, which is expected to increase the translocation of sucrose into the seed of rice plants. This research was conducted by introducing SoSUT1 in rice plants Inpari 14 SS. Transformation using Agrobacterium tumefaciens vector in apical bud explant Indica rice cv. Inpari 14 SS results in 26 events positive rice contains genes SoSUT1. This study aims to elucidate the inheritance of transgene in the next generation and to characterize its effect on the morphology and the yields of a subsequent generation. The study is conducted by planting the seeds of T1 and T2 plants in media containing Hygromycin and using PCR analysis for further analysis. As the results, from 26 events on the T1 plant, only 3 events of T3 plants were confirmed on the T3 plant. The overexpression of the SoSUT1 gene could increase the number of tillers, the number of productive tillers, panicle length, and panicle exit length, also increasing the number of spherical grains, reducing the number of empty grains and increasing the weight of 1000 grains.
Front Cover AB Vol 24 No 2 (2020) ario tutuko
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.%p

Abstract

Guide for Authors AB Vol 24 No 1 (2020) syamsidah rahmawati
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.%p

Abstract

Enhancement Of Yield, Starch, And Amylose Content Of Two Indonesian Cassava Genotypes By Producing Gamma Irradiated-Induced Mutants Enny Sudarmonowati; N Sri Hartati; Supatmi Supatmi
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.95-105

Abstract

As an important carbohydrate-source food crop and raw materials of starch-based industries,  accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield,  high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses  (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding.
Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis) Isa Nuryana; Shanti Ratnakomala; Fahrurrozi Fahrurrozi; Ario Betha Juanssilfero; Ade Andriani; Filemon Jalu Nusantara Putra; Erdiansyah Rezamela; Restu Wulansari; M Iqbal Prawira Atmaja; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.106-113

Abstract

Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of  5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.
Front Cover AB Vol 24 No 1 (2020) syamsidah rahmawati
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.%p

Abstract

Editorial Boards AB Vol 24 No 2 (2020) ario tutuko
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.%p

Abstract

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