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Sains Natural: Journal of Biology and Chemistry
Published by Universitas Nusa Bangsa
ISSN : 20863446     EISSN : 2621508X     DOI : https://doi.org/10.31938/jsn
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
Jurnal Sains Natural is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication with the objectives to explore the knowledge about natural sciences. This journal incorporates not only all branches of chemistry and biology, but also sub-disciplines like Biochemistry, Polymer, Agricultural chemistry, Environmental chemistry, etc.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 9 No. 2 (2019): Sains Natural" : 5 Documents clear
SERANGGA HAMA TERKINI YANG MENYERANG TANAMAN SENGON (Falcataria moluccana (Miq.) Berneby & J.W Grimes) DAN JABON (Neolamarckia cadamba (Roxb.) Bosser) Illa Anggraeni; Neo Endra Lelana; Agus Ismanto
JURNAL SAINS NATURAL Vol. 9 No. 2 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.088 KB) | DOI: 10.31938/jsn.v9i2.223

Abstract

The Latest Insect Pests which Attacks Sengon (Falcataria moluccana (Miq.) Berneby & J.W Grimes) and Jabon Trees (Neolamarckia cadamba (Roxb.) Bosser)The current study of pest insect infestation on sengon (Falcataria moluccana (Miq.) Berneby & J.W Grimes)) and jabon (Neolamarckia cadamba (Roxb.) Bosser) plants has been carried out. The aimed of the study was to identify the latest status of the pests that infested sengon and Jabon plants. The research method used were field survey and observation in the laboratory. The survey was carried out in plantations owned by the Unit II Perhutani Unit in East Java as well as the small scale forests in the Kediri (East Java), Ciamis and Garut (West Java) areas. While observations in the laboratory were conducted at the Pest Laboratory of the Center for Forest Research and Development, Bogor. The results showed that pests that infested sengon and Jabon plants were unknown name of sengon shoot borer, Echinothrips sp. (Tripidae; Thysanoptera), horn beetle Oryctes rhinoceros (Scarabaeidae; Coleoptera), wild silkworm Attacus atlas (Saturniidae; Lepidoptera), leaf-sucking pests Lawana sp./Samurus sp. (Flatidae; Homoptera).Keywords: pest insect, sengon plant, jabon plant, the latest.ABSTRAKPenelitian tentang serangga hama terkini yang menyerang tanaman sengon (Falcataria moluccana) dan jabon (Neolamarckia cadamba) di hutan tanaman, bertujuan untuk menginventarisir jenis-jenis hama yang menyerang tanaman sengon dan jabon saat kini. Metode penelitian yang digunakan adalah metode survei dan pengamatan di laboratorium. Survei dilakukan di hutan tanaman milik Perum Perhutani maupun hutan milik rakyat di daerah Kediri, Ciamis dan Garut. Pengamatan di laboratorium dilakukan di Laboratorium Hama Pusat Penelitian dan Pengembangan Hutan, Bogor. Hasil penelitian menunjukkan bahwa hama yang menyerang tanaman sengon dan jabon adalah hama penggerek pucuk sengon (Pyralidae; Lepidoptera),hama Echinothrips sp. (Tripidae; Thysanoptera), kumbang tanduk Oryctes rhinoceros (Scarabaeidae; Coleoptera), ulat sutera liarAttacus atlas(Saturniidae; Lepidoptera), hama pengisap daun Lawana sp./Sanurus sp. (Flatidae; Homoptera).Kata kunci: serangga hama, tanaman sengon, tanaman jabon
EKSTRAKSI RUMPUT LAUT Gelidium sp. MENJADI BAKTO AGAR SEBAGAI PEMADAT MEDIA PERTUMBUHAN MIKROBA Zachra Resha Shantika; Srikandi Srikandi; RTM Sutamihardja
JURNAL SAINS NATURAL Vol. 9 No. 2 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.797 KB) | DOI: 10.31938/jsn.v9i2.234

Abstract

Extraction of Seaweed Grass Gelidium Sp. Become A Bakto For A Microbic Growth Media Competition      Red seaweed is seaweed that produces gelatin one example is Gelidium sp. The product that has high economic value is bakto agar which is widely used in the field of microbiology, namely as a microbial culture media compactor. The use of bacto so that it will increase domestically, so as to meet the needs of bakto so that in the country with the expected quality classification with bakto so that the commercial is carried out this research. Agar yield value of seaweed type Gelidium sp. produced an average of 26.69%. The proximate test results for the parameters of water content, levels of ash, insoluble ash and sulfate in bakto so that the research is 6.89%, 1.67%, 0.0235%, and 1.31% respectively. The results of testing the levels of protein, fat, carbohydrate and gel strength at bacto so that respectively are 1.72%, 0.05%, 32.87% and 214.45 (g / cm2). The results of testing the mineral metal levels of calcium, magnesium and potassium were obtained at bakto so that they were 1806.06 mg / kg, 680 mg / kg and 338.22 mg / kg respectively. The results of testing the levels of trace elements of iron and copper were obtained at 8.75 mg / kg and 2.10 mg / kg. The results of microbiological testing using the total plate number method using Bacillus subtilis and Eschericia coli bacteria were found to be 8.6 x 106 colonies / mL and 2.81 x 107 colonies / mL. Based on the observation of bacterial morphology by comparing it with bakto so that the commercial standard in general is not much different. So based on the results of proximate and microbiological testing, bakto so that this research can be used as a medium for bacterial growth.Keywords: Gelidium sp., Agar, media and bacteriaABSTRAK      Rumput laut merah merupakan rumput laut yang menghasilkan agar-agar salah satu contohnya adalah Gelidium sp. Produk agar yang memiliki nilai ekonomis tinggi adalah bakto agar yang banyak digunakan dalam bidang mikrobiologi yaitu sebagai pemadat media kultur mikroorganisme. Pemanfaatan bakto agar di dalam negeri semakin meningkat, sehingga untuk mencukupi kebutuhan bakto agar di dalam negeri dengan klasifikasi mutu yang diharapkan sama dengan bakto agar komersial maka dilakukan penelitian ini. Nilai rendemen agar dari rumput laut jenis Gelidium sp. dihasilkan rata-rata sebesar 26.69%. Hasil pengujian proksimat untuk parameter kadar air, kadar abu, abu tak larut asam dan sulfat pada bakto agar penelitian ini berturut-turut adalah sebesar 6,89%, 1,67%, 0,0235%, dan 1.31%. Hasil pengujian kadar protein, lemak, karbohidrat dan kekuatan gel pada bakto agar berturut-turut adalah sebesar 1,72%, 0,05%, 32,87% dan 214,45 (g/cm2). Hasil pengujian kadar logam mineral kalsium, magnesium dan kalium didapatkan pada bakto agar berturut-turut adalah sebesar 1806,06 mg/kg, 680 mg/kg dan 338,22 mg/kg. Hasil pengujian kadar logam trace elements besi dan tembaga didapatkan sebesar 8.75 mg/kg dan 2,10 mg/kg. Hasil pengujian mikrobiologi dengan metode angka lempeng total dengan menggunakan bakteri Bacillus subtilis dan Eschericia coli didapatakan sebesar 8,6 x 106 koloni/mL dan 2,81 x 107 koloni/mL. Berdasarkan hasil pengamatan morfologi bakteri dengan membandingkannya dengan bakto agar standar komersial secara garis besar tidak jauh berbeda. Sehingga berdasarkan hasil pengujian proksimat dan mikrobiologi, bakto agar penelitian ini dapat digunakan sebagai media pertumbuhan bakteri.Kata kunci: Gelidium sp., Agar, media dan bakteri
OPTIMASI PRIMER SINGLE NUCLEOTIDE AMPLIFIED POLYMORPHYSM (SNAP) PADA GEN BRASSINOSTEROID (BRI) KELAPA SAWIT Roberdi Roberdi; Ogi Ajitiyo Ramadhan
JURNAL SAINS NATURAL Vol. 9 No. 2 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.448 KB) | DOI: 10.31938/jsn.v9i2.265

Abstract

Optimisation of Single Nucleotide Amplified Polymorphism (SNAP) Primers in Brassinosteroid (BRI) Gene of Oil Palm Oil palm varieties that have high yields still have a high rate of stem height increase, and it has an impact on difficulties at harvest. Brassinosteroid (BRI) is one of the growth hormones that regulate stem height increase. In this study, the sample used is three types of oil palm, namely E. guineensis which represents the height growth of normal oil palm stems, E. oleifera which represents slow stem height growth, and the result of crossing or hybrid Eo x Eg which is expected to inherit the traits between the two the crossed parent. Sequencing was carried out on samples using a specific primer for the BRI gene that triggers elongation and division of stem cells. The Single Nucleotide Amplified Polymorphisms (SNAP) primers used in the analysis process need to find the optimum temperature to obtain the optimum PCR conditions. Four primers of Single Nucleotide Amplified Polymorphism (SNAP) that get the optimum temperature with a mixture of normal reagents are BRI 69 Alt-Rev and BRI 562 Ref-Rev at 55oC, BRI 1206 Alt-Rev at 57oC and BRI 1206 Ref-Rev at 58oC. Two primers require additional MgCl2 in the reactant mixture, namely BRI 69 Ref-Rev at 55oC and BRI 2115 Alt-Rev at 56oC.Keywords: Oil palm, Brassinosteroid, Primer, DNA, GenesABSTRAK Varietas-varietas kelapa sawit yang memiliki daya hasil tinggi umumnya masih memiliki laju pertambahan tinggi yang relatif cepat dan berakibat pada kesulitan saat panen. Brassinosteroid (BRI) merupakan salah satu hormon pertumbuhan yang mengatur pertambahan tinggi batang. Pada studi ini, Sampel yang digunakan adalah tiga jenis kelapa sawit yaitu sampel E. guineensis yang mewakili pertumbuhan tinggi batang kelapa sawit normal, E. oleifera  yang mewakili pertumbuhan tinggi batang lambat, dan hasil persilangan atau hibrida Eo x Eg yang diharapkan mewarisi sifat antara kedua induk yang disilangkan. Sekuensing dilakukan pada sampel menggunakan primer spesifik gen BRI yang memicu pemanjangan dan pembelahan sel batang. Primer Single Nucleotide Amplified Polymorphisms (SNAP) yang digunakan dalam proses analisis perlu dicari suhu optimumnya agar didapatkan kondisi PCR yang optimum sehingga dihasilkan produk PCR yang spesifik. Empat primer Single Nucleotide Amplified Polymorphism (SNAP) yang mendapatkan suhu optimum dengan campuran pereaksi normal yaitu BRI 69 Alt-Rev dan BRI 562 Ref-Rev pada suhu 55oC, BRI 1206 Alt-Rev pada suhu 57oC dan BRI 1206 Ref-Rev pada suhu 58oC. Dua primer membutuhkan tambahan MgCl2 pada campuran pereaksinya yaitu BRI 69 Ref-Rev pada suhu 55oC dan BRI 2115 Alt-Rev pada suhu 56oC.Kata Kunci: Kelapa sawit, Brassinosteroid, Primer, DNA, Gen
ISOLASI DAN IDENTIFIKASI JAMUR ENDOFIT PADA UMBI TALAS (Colocasia esculenta (L.) Schoot) Fitria Dewi Sulistiyono; Siti Mahyuni
JURNAL SAINS NATURAL Vol. 9 No. 2 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.386 KB) | DOI: 10.31938/jsn.v9i2.235

Abstract

Isolation and Identification of Endofit Fungus in Taro Tubers (Colocasia esculenta (L.) Schoot)Endophytic fungi are fungi the living and associated in plant tissue. Association the endophytic and host are mutualism. The ability of endophytic producing a secondary compound is an opportunity to be developed. The aims of this research is isolate and identify the types of endophytic fungi in tubers of taro. The methode used microscopis and macroscopis idetify. The result types of endophytic fungi in tubers of taro are Aspergillus, Sclerotium, Fusarium, Mucor and Rhizopus.Keyword: Taro, Idetify, Endophytic fungiABSTRAKMikroorganisme endofitik adalah mikroorganisme yang hidup dan berasosiasi di dalam jaringan tanaman inang. Asosiasi yang terjadi umumnya bersifat mutualisme. Kemampuan mikroorganisme endofitik memproduksi senyawa metabolit sekunder sesuai dengan tanaman inangnya merupakan peluang yang sangat baik. Pemanfaatan mikroorganisme endofit diharapkan dapat melestarikan tanaman inangnya yang membutuhkan waktu untuk tumbuh dan berkembang.  Tujuan dari penelitian ini adalah mengisolasi dan mengidentifikasi jenis-jenis jamur endofit yang terdapat pada umbi talas. Metode yang digunakan adalah identifikasi secara mikroskopis dan makroskopis. Hasil identifikasi dengan pengamatan secara makroskopis dan mikroskopis diperoleh genus Aspergillus, , Sclerotium, Fusarium, Mucor, dan Rhizopus.Kata kunci : Talas, Identifikasi, Kapang endofit
FITOREMEDIASI TANAH TERCEMAR LOGAM BERAT Cd DENGAN MENGGUNAKAN TANAMAN HANJUANG (Cordyline fruticosa) Novie Eka Permata Sari; Nurlela Nurlela; Supriyono Eko Wardoyo
JURNAL SAINS NATURAL Vol. 9 No. 2 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.785 KB) | DOI: 10.31938/jsn.v9i2.230

Abstract

Phytoremediationn of Cadmium (Cd) Contaminated Soil using Hanjuang Plants (Cordyline fruticosa)Phytoremediation of Cadmium (Cd) contaminated soil using ornamental plants is one method that applicable and environmental friendly in the process absorption of metal in the soil. This study aims to determine the effectiveness of phytoremediation and the potential of hanjuang plants as phytoremediation agents for cadmium metals. Hanjuang plants are planted on soil media which is previously added to heavy media as pollutants, namely Cd(NO3)2 with a concentration of 0; 50; 100; 150 mg/Kg for 42 days. Measurement of Cd concentration uses Inductively Coupled Plasma Optical Emission Spectrometry instrument. The results showed that hanjuang plants could accumulate cadmium metals in the roots. The highest accumulation is found in the roots of plants at 150 mg/Kg concentration and the result is 36.2167 mg/Kg, whereas in the stems and leaves of plants is not found heavy metal absorptions. Hanjuang plants contaminated with cadmium metal have a bioconcentration value (BCF)<1 so that they are included in the metal excluder group or low accumulator. The highest distribution of cadmium metal concentration is in the roots with transport factor (TF)<1 so that hanjuang plants are included in phytoremediation plants with their metal absorption which mechanism is phytostabilization.Keywords : Cordyline fruticosa, Phytoremediation, Cadmium, ICP-OESABSTRAKFitoremediasi tanah tercemar logam Kadmium (Cd) menggunakan tanaman hias merupakan salah satu metode yang aplikatif dan ramah lingkungan dalam proses penyerapan logam di dalam tanah. Penelitian ini bertujuan untuk mengetahui efektivitas fitoremediasi dan potensi tanaman hanjuang sebagai agen fitoremediasi dalam menyerap logam kadmium. Tanaman hanjuang ditanam pada media tanah yang sebelumnya media tanah tersebut ditambahkan logam berat sebagai pencemar yaitu Cd(NO3)2 dengan variasi konsentrasi 0; 50; 100; 150 mg/Kg selama 42 hari. Pengukuran konsentrasi Cd menggunakan instrumen Inductively Coupled Plasma Optical Emission Spectrometry. Hasil penelitian menunjukkan tanaman hanjuang dapat mengakumulasi logam kadmium pada bagian akar. Akumulasi tertinggi ditemukan pada bagian akar tanaman pada konsentrasi 150 mg/Kg sebesar 36,2167 mg/Kg, sedangkan pada bagian batang maupun daun tanaman tidak ditemukan adanya penyerapan. Tanaman hanjuang yang terkontaminasi oleh logam kadmium memiliki nilai biokonsentrasi (BCF)<1 sehingga termasuk ke dalam kelompok metal excluder atau akumulator rendah. Distribusi konsentrasi logam kadmium paling tinggi terdapat pada bagian akar dengan faktor transport (TF)<1 sehingga tanaman hanjuang termasuk ke dalam tanaman fitoremediasi dengan mekanisme penyerapan logamnya yaitu fitostabilisasi.Kata kunci : Cordyline fruticosa, Fitoremediasi, Kadmium, ICP-OES.

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