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INDONESIA
JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION)
Published by Universitas Andalas
ISSN : 25800604     EISSN : 26213141     DOI : -
Core Subject : Agriculture, Social,
Arjuna Subject : -
Articles 6 Documents
Search results for , issue "Vol. 7 No. 2 (2023): December 2023" : 6 Documents clear
Chemical Composition of Cymbopogon flexuosus and C. winterianus Essential Oils and Their Insecticidal Potential Against the Coffee Berry Borer Hypothenemus hampei: Venkateaha Venkatesha, Melally Giddegowda; Kiran, Rudramuni
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.65-77.2023

Abstract

Hypothenemus hampei is the most important insect pest of coffee in the world. H. hampei is controlled by using endosulfan and methyl bromide. However, using these two chemicals may pose pesticide resistance, bioaccumulation, and a threat to human health and the environment. This study evaluated the chemical compositions of essential oils from Cymbopogon flexuosus and C. winterianus and their insecticidal potential against H. hampei as an alternative to endosulfan and methyl bromide. The chemical profiles of the two essential oils showed that both constituted biochemically distinct monoterpenes and sesquiterpenes. The main compounds identified in C. flexuosus were citral, geraniol, and isoforms, representing 76.45% of the total composition, and other active ingredients were citronella, caryophyllene, citronellyl acetate, cadinene, linalool, and limonene. The composition of C. winterianus was citronella, nerol, and citronellol as principal components, corresponding to 66.47%, along with other active ingredients elemol, geranyl acetate, citronellyl acetate, germacrene, and limonene. Differential sensitivity of H. hampei adults was observed in the bioassay of two oils, where a dose-dependent increase in mortality was observed. Among two concentrations of both essential oils, 100% mortality was observed at 500 and 1000 ppm within 48 and 24 hours of treatment, respectively. The significant biological activity of both Cymbopogon species oils indicated a potential source for further development of botanical pesticides as an effective, useful alternative for currently used toxic insecticides. Additionally, essential oils of C. flexuosus and C. winterianus and their active ingredients are classified as non-residual insecticides and are eligible for a pesticide registration exemption.
Identification and Characterization of Fungi Associated with Leaf Spot Disease of Rubber Trees (Hevea brasiliensis) in Pahang, Malaysia Zainudin, Nur Ain Izzati Mohd; Azhar, Nur’ain; Rosli, Muhamad Najmi Haikal; Nordin, Nor Aisyah Md
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.89-102.2023

Abstract

Rubber trees are important sources of agricultural income as they are utilized for lumber and latex. Like other crops, they are susceptible to numerous fungal pathogens, especially on their leaves, the most important plant part. Therefore, this study was conducted to identify the fungi associated with leaf spot disease on the leaves of rubber trees. This study aimed to characterize fungi associated with diseased rubber leaves and determine the causative agent of leaf spot disease. A total of 20 fungal isolates were obtained and purified from rubber leaves collected during sampling in rubber plantations in Pahang, Malaysia. All the isolated fungi were identified as Colletotrichum siamense (2 isolates), Diaporthe sp. (5 isolates), Lasiodiplodia pseudotheobromae (1 isolate), L. theobromae (7 isolates) and Neoscytalidium sp. (5 isolates). Based on a pathogenicity test, it was found that nine isolates were pathogenic towards the leaves of rubber, which were L. theobromae, L. pseudotheobromae, and C. siamense. The lesions on the leaves displayed chlorosis with varying necrotic lesion sizes after 21 days post-inoculation. The isolates of the species L. theobromae were the most prevalent, indicating it is more widespread in the region than other species. The findings present data on the potential pathogen of rubber plants and are important to understand further the potential risks and effects of the pathogen in rubber plantations
Biological dan Molecular Characterization of Papaya Ringspot Virus from Bogor District, Indonesia Riska, Riska; Husada, Eko Darma; Budiyanti, Tri; Jumjunidang, Jumjunidang
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.78-88.2023

Abstract

Management of PRSV using cross-protection and transgenic plants has been hampered due to varying PRSV gene sequences. Therefore, the characterization of new PRSV isolates could help design the region needed for region-specific management practices. The study aimed to characterize the typical PRSV isolates found in Bogor biologically and molecularly. The study was conducted in 2 stages: host range study of 2 isolates (Sukaraja and Cijeruk) on five species with six plants for replication, and RT-PCR analysis amplified coat protein (CP) region using PRSV326 and PRSV800 primer pair. The results showed that Sukaraja isolate produced systemic symptoms in papaya (Carica papaya L) cv. Merah Delima in the form of leaf lamina becoming pale and wilting, leaf malformation, open veins, and striped patterns on the leaves, while the Cijeruk isolate causes symptoms of leaf blistering, mosaicism, leaf malformation, wilted lamina, and striped patterns on the leaves. These two isolates did not cause symptoms on eggplant (Solanum melongena) and chickpea (Vigna unguiculata) but produced striped leaf patterns and pale lamina symptoms on bitter melon (Momordica charantia) and melon (Cucumis melo) leaves. RT-PCR analysis was able to amplify ±475 bp of DNA. The two DNA isolates had a homology percentage of 97.7% with PRSV isolates from Thailand, ranging between 93.07-99.68% with the Kulon Progo and Nganjuk isolates. Based on phylogenetic analysis, the Cijeruk isolate was in the same branch as the Indonesian isolate, while the Sukaraja isolate was a separate branch and closely related to isolates from Oklahoma and Malaysia.
New Record of the Egg-larval Parasitoid, Chelonus formosanus Sonan of Fall Armyworm, Spodoptera frugiperda (J.E. Smith) in the Philippines Calcetas, Orlando Adona; Joshi, Ravindra Chandra; Gupta, Ankita; Ranjith, Avinjikkattu Parambil; Madrid, Mary Ann; Fameronag, Joash
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.103-114.2023

Abstract

The egg-larval parasitoid on fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) have not reported in the Philippines. This paper summarizes the global biological information on two species of Chelonus and their role in the regulation of FAW. The FAW egg masses and larvae were collected in the corn plantations inside the Lipa Agricultural Research Station, Marawoy, Lipa City, Batangas. The collected egg masses and larvae were reared at the Entomology Laboratory of the Department of Agriculture-Regional Crop Protection Center IVA. The larvae of FAW were observed for the emergence of parasitoids. Two cocoons of the parasitoid from the rearing cages were obtained for species determination. Three adults from the emerged parasitoids, each male and female, were initially preserved and labeled. Color images of habitus and other morphological characters of the parasitoid were taken using a DSLR camera attached to an Olympus SZ61-60 microscope. This research found two species that emerged from FAW: Chelonus formosanus and the new record of C. semihyalinus. We also highlight the need for more research in the Philippines on exploring the diversity of indigenous natural enemies, the development of mass-rearing techniques, and their utilization to manage fall armyworms.
Population Distribution of Rats (Rattus argentiventer) and the Damage Intensity on Rice and Other Crops in Pleret, Yogyakarta, Indonesia Brotodjojo, RR Rukmowati; Lazuardi, Ikhsan; Suprihanti, Antik
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.115-126.2023

Abstract

Rice field rats is one of the main pests of rice in Indonesia and other Asian countries that can cause a crop failure. The availability food sources might affect the rat foraging in the field. The research aimed to determine the distribution of rat population and their nests in the field with different crop and to examine the level of crop damage caused by rats. The research was conducted from January to April 2023 in Pleret district, Yogyakarta, Indonesia. The 1000 m2 TBS (Trap Barrier System) was installed in the middle of the 225 ha observation area to trap rats. Crop damage inside TBS was observed using a diagonal sampling method. Crop damage outside TBS was observed using the line transect method in the 4 cardinal directions with a distance of 250m, 500m, and 750m from TBS. Rat nest’s locations were mapped using GPS. The results showed that the number of rats trapped was low in the early and late stage of rice growth and peaked when the rice aged 12 weeks after planting (WAP). Rice crop damage inside TBS occurred when rice entered 12 WAP. There was no damage on rice crops around the TBS. Rats also attacked corn and sugarcane which caused heavy damage to these crops. There were 157 active holes found in 225ha observation area and they mostly were found in rice field bunds, irrigation embankments, and vacant land. This study implied that understanding the distribution of rat’s population and their nest will support the rat control.
Virulence of Five Isolates of The Entomopathogenic Fungus, Metarhizium anisopliae, Against Brown Planthopper (Nilaparvata lugens) Trizelia, Trizelia; Rahma, Haliatur; Syahrawati, My
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.127-133.2023

Abstract

The brown planthopper (Nilaparvata lugens) is one of the main pests of rice plants. This pest attack can cause crop failure or puso. Biological control of this pest can be carried out using the entomopathogenic fungus, Metarhizium anisopliae. The ability of this fungus to control pests is influenced by the source of the isolate. The purpose of this study was to obtain isolates of M. anisopliae, which is virulent to N. lugens. This study used an experimental method with a completely randomized design (CRD) in six treatments and five replications. There were five isolates of M. anisopliae used and one control. M. anisopliae was applied to nymphs and adults of N. lugens with a concentration of 108 conidia/mL. The research revealed that all M. anisopliae isolates tested were virulent against N. lugens nymphs and adults. The most virulent isolate for the two stages of BPH was Met3B, which was collected from the leek rhizosphere. The isolate caused an adult mortality rate of 52%, nymph mortality of 60% in 10 days after application, and adults formed after application was only 37.33%.

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