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Observation Test of Durian (Durio zibethinus Murr.) from Several Regions in West Sumatera Indriyani, Ni Luh Putu; Ihsan, Farihul; Hadiati, Sri; Budiyanti, Tri; Riska, Riska; Jumjunidang, Jumjunidang; Mansyah, Ellina; Muas, Irwan
Bioscience Vol 8, No 2 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i2.129070

Abstract

The open pollinating of durian produces diversity that allows for indigenous selection. The aim of the research is to raise the potential of the genetic resources of West Sumatran durian to become a new superior variety. Observation tests were carried out in Sijunjung, Tanah Datar, Padang Pariaman, Sawahlunto, and Pesisir Selatan The variables observed were qualitative and quantitative characters, using the descriptor for durian and guidelines for preparing horticultural variety descriptions. Observations of qualitative and quantitative characters were carried out on plants, stems, leaves, flowers and fruit. From the results of the observation test, 4 new superior varieties have been registered, namely Kalumpang Sijunjung with Minister of Agriculture Decree No: 039/Kpts/SR.120/ D.2.7/6/2014, Tambago Sungai Tarab with Minister of Agriculture Decree No:015/Kpts/SR.120/D.2.7/2/2016, Kunik Tandikek with Minister of Agriculture Decree No: 258/Kpts/SR.130/D/III/2021, Sawah Kubang with Minister of Agriculture Decree No:225/Kpts/PV.240/D /VI/2023 and one prospective of durian variety of Pesisir Selatan which which is currently being processed in registering local varieties. In order for new varieties to develop, there needs to be a development program from local governments to stakeholders.  Apart from that, activities to increase the potential of durian genetic resources in West Sumatera need to continue.
Morphological Characterization and Fusarium Wilt Resistance of Triploid Banana Mutant Line (Musa acuminata Cola) Ishak, Ishak; Jumjunidang, Jumjunidang
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.3900

Abstract

Br 23 banana mutant line derived from irradiated gamma-ray from pisang ambon cultivar was triploid (AAA) and sterile. Therefore, application of conventional breeding to improve the agronomic characteristics of bananas requires strenuous effort. Morphological and agronomical characterizations of mutant line were observed during the generative stage on the Fusarium hotspot. Sixteen qualitative characters were observed, and each of which related to color was standardized using RHS color chart. Based on these qualitative characteristics, it was confirmed that pisang Ambon belongs to the acuminata group. Several agronomical characteristics of Br 23 banana mutant line, such as the number of hands/bunch, the weight of fruits/hand, and single fruit weight, were observed. The experimental results on banana production showed that the first harvest could reach 26 t/ha. Unlike from the control plant, the Br 23 mutant line showed tolerant characteristics to Fusarium TR4. The evaluation of susceptibility to Fusarium wilt was analyzed using the Vegetative Compatibility Group (VCG) method with isolate no. 01236/16. These susceptible plants were planted nearby the Br 23 banana mutant line. In early 2019, this Br 23 banana mutant line was registered as a new mutant variety under the name of PIRAMA I.
Biological dan Molecular Characterization of Papaya Ringspot Virus from Bogor District, Indonesia Riska, Riska; Husada, Eko Darma; Budiyanti, Tri; Jumjunidang, Jumjunidang
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.78-88.2023

Abstract

Management of PRSV using cross-protection and transgenic plants has been hampered due to varying PRSV gene sequences. Therefore, the characterization of new PRSV isolates could help design the region needed for region-specific management practices. The study aimed to characterize the typical PRSV isolates found in Bogor biologically and molecularly. The study was conducted in 2 stages: host range study of 2 isolates (Sukaraja and Cijeruk) on five species with six plants for replication, and RT-PCR analysis amplified coat protein (CP) region using PRSV326 and PRSV800 primer pair. The results showed that Sukaraja isolate produced systemic symptoms in papaya (Carica papaya L) cv. Merah Delima in the form of leaf lamina becoming pale and wilting, leaf malformation, open veins, and striped patterns on the leaves, while the Cijeruk isolate causes symptoms of leaf blistering, mosaicism, leaf malformation, wilted lamina, and striped patterns on the leaves. These two isolates did not cause symptoms on eggplant (Solanum melongena) and chickpea (Vigna unguiculata) but produced striped leaf patterns and pale lamina symptoms on bitter melon (Momordica charantia) and melon (Cucumis melo) leaves. RT-PCR analysis was able to amplify ±475 bp of DNA. The two DNA isolates had a homology percentage of 97.7% with PRSV isolates from Thailand, ranging between 93.07-99.68% with the Kulon Progo and Nganjuk isolates. Based on phylogenetic analysis, the Cijeruk isolate was in the same branch as the Indonesian isolate, while the Sukaraja isolate was a separate branch and closely related to isolates from Oklahoma and Malaysia.