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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 10 Documents

Search results for , issue "Vol 6, No 2 (2011): (December 2011)" : 10 Documents clear

THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei Nunak Nafiqoh; Poh-Shing Chang; Yu-Chi Wang
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This study examined the effect of two Lactobacillus bacteria on protease activity and growth rate of Litopenaeus vannamei. An experiment was conducted to examine protease activity and growth rate. The experiment consisted of two treatment tanks, the first tank was provided with artemia immersed in 2.6 x 1016 cfu/mL of bacteria solution, the second tank served as the control tank. After 20 days, the L. vannamei in the tank that received Lactobacillus have significantly different in growth, survival rate and protease activity (P<0.05) compared to the control, but no significant difference between Lactobacillus casei and Lactobacillus plantarum treatments. Within the digestive organ, protease activity of hepatopancreas and stomach demonstrated significant higher activity (P<0.05) compared to the intestine.

MOLECULAR IDENTIFICATION AND CLONAL RELATION OF ATYPICAL ISOLATE Aeromonas salmonicida USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) Riza Priyatna; Indaryulianto Indaryulianto; Kurniasih Kurniasih
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Aeromonas salmonicida is responsible in many cases of furunculosis outbreaks resulting in economic loss of freshwater aquaculture. Four isolates of A. salmonicida have been isolated from fish collected in four different regions in Indonesia and its clonal relation has yet to be determined. In the research, A. salmonicida isolates and ATCC atypical isolate as the control had been checked for their clonal relation using Restriction Fragment Length Polymorphism (RFLP) method in which restriction enzyme of AluI, HaeIII, MboI, and EheI were used. PCR test results using the primers 16S rDNA amplicon gave a positive response to the 1300 bp band. The result of RFLP analysis showed that A. salmonicida atypical isolates from Indonesia represent subspecies smithia except isolates from C. macropomum in Yogyakarta in MS and 16S rDNA regions. Isolates from Jambi, Pontianak and Semarang showed a slight variation on enzyme restriction sites. Isolates number 2, 3, 4 and 5 had the same restriction sites using AluI enzyme with MS primer. The restriction enzymes that could give the best result for RFLP method of A. salmonicida were HaeIII, MboI and EheI.

THE EFFECTIVITY TEST OF SHEEP RUMEN LIQUOR ENZYME ADDED TO PALM KERNEL MEAL ON ITS DECREASE OF CRUDE FIBER AND APPARENT DIGESTIBILITY COEFFICIENT FOR CATFISH Pangasius hypophthalmus DIET Wahyu Pamungkas; Dedi Jusadi; Nur Bambang Priyo Utomo
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Two experiments were conducted to evaluate the hydrolysis of fiber content in palm kernel meal (PKM) by sheep rumen liquor enzyme and to know the apparent digestibility coefficient of hydrolyzed PKM for catfish Pangasius hypophthalmus. The first trial examined effectivity of sheep rumen liquor enzyme to decrease crude fiber content of PKM. The added volume of sheep rumen liquor enzyme was 0, 20, 40, 60, 80, and 100 mL/kg PKM and then it was incubated for 0, 12, and 24 hours. A factorial completely randomized experimental design consisted of 2 variables and triplicates were selected. The second trial was conducted to evaluate the apparent digestibility coefficients of hydrolized PKM for catfish. Apparent digestibility coefficients were determined using chromic oxide indicator added to both reference and test diets. The feed ingredients used in the trial were hydrolyzed PKM (PKMe) and unhydrolyzed PKM (PKM). Ten fishes with weighing around 20 g were used in the trial and held in 80 l tanks. Feces were collected from three replicate groups of fish using a fecal collection column attached to fish rearing tank. PKM hydrolyzed with 100 mL/kg and incubated for 24 hour showed the lowest crude fiber content (6.99%) among the treatments (P<0.05). Apparent digestibility coefficient of hydrolyzed PKM was 57.57% compared with unhydrolyzed PKM 15.31%. Based on the evaluation in those parameters it was concluded that sheep rumen liquor enzyme added to PKM was effective to decrease crude fiber content of PKM and improve apparent digestibility coefficient of PKM for catfish.

THE USE OF REMOTE SENSING, REGRESSION QUANTILES, AND GIS APPROACHES FOR MODELING OF SCALLOP LARVAE: A Case Study in Funka Bay, Hokkaido, Japan I Nyoman Radiarta
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In the development of scallop cultivation in Japan, larvae collection and propagation become an important factor. Although the monitoring program has been conducted, modeling of species distribution is becoming an important tool for understanding the effects of environmental changes and resources management. This study was conducted to construct a model for providing estimation of the scallop larvae distribution in Funka Bay, Hokkaido, Japan using the integration of remote sensing, Regression Quantile (RQ) and Geographic Information System (GIS)-based model. Data on scallop larvae were collected during one year spawning season from April to July 2003. Environmental parameters were extracted from multi sensor remotely sensed data (chlorophyll-a and sea surface temperature) and a hydrographic chart (water depth). These parameters together with larvae data were then analyzed using RQ. Finally, spatial models were constructed within a GIS by combining the RQ models with digital map of environmental parameters. The results show that the model was best explained by using only sea surface temperature. The highest larvae densities were predicted in a relatively broad distribution along with the shallow water regions (Toyoura and Sawara to Yakumo) and the deeper water areas (center of the bay). The spatial model built from the RQ provided robust estimation of the scallop larvae distributions in the study area, as confirmed by model validation using independent data. These findings could contribute on the monitoring program in this region in order to distinguish the potential areas for an effective spat collection.

IMPROVING WATER QUALITY AND PRODUCTIVITY OF TILAPIA (Oreochromis niloticus) USING CONSTRUCTED WETLAND Eri Setiadi; Lies Setijaningsih
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.6.2.2011.107-122

Organic and inorganic pollutants such as N, P, and heavy metals are a serious problem in water bodies (lake, reservoir, river, and stream) and have deleterious effects to pond productivity and human health. These pollutants produced from anthropogenic activities (i.e. industrial, agricultural, and settlement) are released into the water bodies and causing poor water quality. Constructed wetland (CW) is one of the technologies that have the capability to solve such problems. The purpose of this experiment was to examine the efficiency of constructed wetland in terms of reducing pollutants from the stream as a water resource for aquaculture area and increasing fish production. Two kinds of treatments were set up: one fish pond culture with constructed wetland and the other one without constructed wetland (control). Each treatment consisted of three replications. The result showed that water quality in the fish pond with CW was better than the control. Constructed wetland were able to reduce ammonia (NH3-N), nitrite (NO2-N), nitrate (NO3-N), TN, phosphate (PO4-P), TP, Cu, Pb, and As concentrations to 15.00%-88.27%, 9.52%-72.73%, 11.11%-57.14%, 20%-66.67%, 24.44%-80.77%, 12.04%-77.95%, 50.00%-100%, 56.25%-100%, and 46.83%-100%, respectively. Nile tilapia cultured in pond using CW was higher than the control in terms of total length, body weight, and survival rate. The t test revealed that nile tilapia (Oreochromis niloticus) cultured in pond with CW and the control has significant difference (P<0.01) in terms of total length, body weight, and survival rate. This is the evidence that constructed wetland technology is very useful on improving water quality and increasing pond productivity of nile tilapia cultured in the freshwaterpond.

IDENTIFICATION OF MAJALAYA COMMON CARP STRAINS RESISTANT TO KHV INFECTION USING CYCA-DAB1*05 ALLELE AS THE MARKER Alimuddin Alimuddin; Mubinun Mubinun; Ayi Santika; Odang Carman; Irvan Faizal; Komar Sumantadinata
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The Cyca-DAB1*05 allele of major histocompatibility complex class II genes is recently suggested to have a link with the European common carp strain resistant to koi herpesvirus (KHV). In this study, a set of specific primers for Cyca-DAB1*05 was designed and applied as a marker to identify broodstocks of majalaya common carp strain subsequently used as a candidate resistant to KHV infection. From a total of 23 broodstock subjected to PCR analysis, two female and male fish, both having (P) and no Cyca-DAB1*05 (N), were selected and then diallelly mated. Disease resistance of progenies from 10 crosses was determined by a survival analysis in pond rearing and a laboratory challenge-test using cohabitation method. The results have revealed that the average survivals of PxP progenies for pond rearing and KHV challenge test were 86% and 100% higher (P < 0.05) respectively compared to that of NxN fish. Survival rate of PxN/NxP progenies was significantly lower (P < 0.05) than that of PxP fish. Furthermore, PCR analysis showed that almost 91% progenies of PxP crosses seemed to have a KHV resistant gene marker. Thus, this study suggests that the marker is associated with the KHV resistance in majalaya common carp strain, and farming of PxP progenies can be useful to increase common carp production.

PERFORMANCE OF RED TILAPIA IN THREE DIFFERENT LEVELS OF SALINITY Didik Ariyanto; Muslikhin Muslikhin
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Tilapias are indigeneous species to Africa, but interest in their aquaculture potential has led to nearly worldwide distribution of the species within the past fifty years. The most popular species of tilapias cultured in the world is Oreochromis niloticus. Red tilapia, one of tilapia varieties, is a highly valued commodity in global market. Based on this reason, red tilapia culture should be developed to supply the market demand. Tilapias can be culturred in brackishwater ponds due to its tolerant ability to saline water. This study was carried out to know the potential of tilapia especially the NIFI red tilapia cultured in saline water. The seeds of NIFI red tilapia were reared in three levels of salinity, i.e. 10, 20, and 30 ppt with 0 ppt as the control population. The experiment was conducted in wet laboratory for 56 days. The results showed that the difference of salinity level did not affect the performance of NIFI red tilapia, including specific growth rate, survival rate, total biomass, and food conversion ratios. Based on these results, it is suggested that NIFI red tilapia can be cultured in saline waters.

ULTRASTRUCTURAL FEATURE OF KOI HERPESVIRUS (KHV) INFECTED CULTURED KOI FIN (KF-1) CELLS Ketut Mahardika; Masahiro Yasuda
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Koi herpesvirus (KHV), may cause significant morbidity and mortality in common carp (Cyprinus carpio). In the present study, an electron microscopic (EM) was performed on KHV-infected cultured koi fin (KF-1) to document the ultrastructure of the lesions. Viral particles were firstly evident in the nucleus. These viral particles observed as immature capsids and nucleocapsids. Many non-enveloped nucleocapsids have moved from the nucleus into the cell cytoplasm. The formation of subviral particles and virions, which comprised, in turn, an electron dense core, capsids with a hexagonal outline, the tegument was evident in the cytoplasm. And then, the virions with the enveloped tegument budded through the intracytoplasmic membrane. Based on EM results, the definitive pathological change was similar as those in the Family Herpesviridae.

THE EFFECT OF AERATION ON THE DISTRIBUTION OF MICROALGAE CONCENTRATE Ludi Parwadani Aji
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Microalgae concentrates (paste) can be used as an alternative feed to replace live microalgae for aquaculture due to its nutritional value and convenience. However, the clumping of cells and negative buoyancy of algae concentrate can affect bivalve culture as bivalves only capture particles suspended in the water and ingest a certain size range of particles. This study investigated the effects of aeration on the distribution of algae particles in a 500 L rearing tank. The results indicated that the number of algae paste particles was higher in the aerated tanks compared to the non-aerated tanks over 24 hours. Time lapsed had a significant effect on the number of particles after the addition of algae concentrate into the tanks. The number of particles in the aerated and non-aerated tank was relatively stable from 1 to 10 hours and then declined after 24 hours. Furthermore, tank depth had no significant effect on the number of particles distributed as the number of particles did not differ significantly at three different depths within the culture tanks.

IN VITRO ANTAGONISTIC ACTIVITIES OF INDONESIAN MARINE SPONGE AAPTOS AAPTOS AND CALLYSPONGIA PSEUDORETICULATA EXTRACTS AND THEIR TOXICITY AGAINST Vibrio spp. Rosmiati Rosmiati; Habsah Mohamad; Tengku Sifzizul Tengku Muhammad; Najiah Musa; Aziz Ahmad; Noraznawati Ismail; Farida Mohamad; Nurhidayah Nurhidayah
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.

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Home Page

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Location Kota adm. jakarta pusat, Dki jakarta INDONESIA

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Contact Name
Ofan Bosman

Contact Email
Ofan Bosman

Phone
-

Journal Mail Official
iaj.puslitbangkan@gmail.com

Editorial Address
-

Location
Kota adm. jakarta pusat,

Dki jakarta

INDONESIA