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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 334 Documents

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EFFECTS OF DIFFERENT SALINITY LEVELS ON PHYSIOLOGICAL AND HEMATOLOGICAL RESPONSE OF ROCK BREAM Oplegnathus fasciatus Vitas Atmadi Prakoso; Jun Hyung Ryu; Byung Hwa Min; Rudhy Gustiano; Young Jin Chang
Indonesian Aquaculture Journal Vol 11, No 2 (2016): (December, 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Rock bream Oplegnathus fasciatus is one of economically important marine fish species in East Asia. However, lack of information about the salinity tolerance of rock bream related to its physiological response made this issue were needed to be studied. The present study was conducted to determine the effects of different salinity levels on physiological and hematological response of rock bream in order to obtain its salinity tolerance. Twelve rock breams (total length: 26.9 ± 0.6 cm, body weight: 477.3 ± 61.9 g) were used for experiments. Four experimental groups with three replications were conducted to measure the effects of salinity (5, 15, 25, and 35 practical salinity unit (psu)) on physiological and hematological response of rock bream. Fish were stocked into the chamber inside the closed recirculation system. At the end of each experiment, blood samples were collected. The study revealed that lower salinity exposure had tendency to decrease the physical and chemical properties of blood in rock bream. The value of Na+, Cl-, Ca, Mg, and osmolality showed tendency to decrease with lowering salinity, while cortisol and glucose showed tendency to increase from 35 psu to low salinity environment, indicating the enhancement of fish stress and resulted in fish mortality at 5 psu. The lowest cortisol value was 76.3 ng/mL in 25 psu, and the highest value was 188.8 ng/mL in 5 psu. Meanwhile, the lowest glucose value was 35.3 mg/dL and the highest value was 166.7 mg/dL (P<0.05). Results indicate that rock bream could tolerate lower salinity up to 15 psu.

DEVELOPMENT OF PRIMARY CELL CULTURE FROM TAIL EPIDERMAL TISSUE OF KOI CARP (Cyprinus carpio koi) Lila Gardenia; Isti Koesharyani
Indonesian Aquaculture Journal Vol 9, No 1 (2014): (June 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Primary cell culture from tail epidermal tissue of koi carp (Cyprinus carpio koi) was developed. Cells were grown in Leibovits-15 medium supplemented with 20% fetal bovine serum and antibiotics (Penicillin/Streptomycin and Kanamycin). Cell growth was observed in a range of incubation temperature (17oC±2oC, 22oC±2oC, 27oC±2oC, and 32oC±2oC) in order to determine the optimum temperature. The cells were able to grow at a range of temperature between 17oC to 32oC with optimal growth at 22oC. Primary cells infected with koi herpes virus produced typical cytopathic effects characterized by severe vacuolation and deformation of nuclei, which is consistent with those of previous reports. Artificial injection experiment by using supernatant koi herpes virus SKBM-1 isolate revealed that it could cause 90% mortality in infected fish within two weeks. PCR test with Sph I-5 specific primers carried out with DNA template from supernatant virus, pellet cell, and gills of infected fish showed positive results in all samples (molecular weight of DNA target 290 bp). The cells were found to be susceptible to koi herpes virus and can be used for virus propagation.

GENETIC DIVERSITY OF Vibrio harveyi ISOLATED FROM TIGER PRAWN Penaeus monodon HATCHERIES AND GROW OUT PONDS Muliani Muliani; Ince Ayu Khairana Kadriah; Andi Parenrengi; Sulaeman Sulaeman
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

ibrio harveyi is known as one among the most harmful bacteria infecting tiger prawn at every stage of its life’s. The present research was aimed to reveal the genetic diversity of Vibrio harveyi isolated from tiger prawn (Penaeus monodon) culture. The samples of bacteria were collected from hatchery (brood-stock, larvae, natural feed, artemia, and larval rearing water) and grow-out (juveniles, water, shrimp, sediment, plankton, crab, mollusc, microalgae, and wild fish). The taxonomic identification of Vibrio spp.was performed based on the physiological and biochemical characteristic following the isolation by Thiosulphate Citrate Bile-salt Sucrose Agar (TCBSA) media. Amplified Ribosomoal DNA Restriction Analysis (ARDRA) for 16S-rRNA analysis and Macrorestriction Fragment Length Polymorphism (MLFP) analysis using Pulsed Field Gel Electrophoresis (PFGE) were applied to reveal the genetic diversity of V. harveyi. According to the taxonomic identification, of 361 isolates of Vibrio spp., 129 isolates (35.7%) were identified as V. harveyi. The result of ARDRA analysis showed that the 16S-rRNA gene of V. harveyi digested by RsaI and HhaI enzyme, each generated three and four identical fragments respectively for the all samples. These meaned that ARDRA could not reveal any genetic variation on V. harveyi. The size range of all DNA fragment was less than 500 bp. This result indicated that the high genetic diversity of V. harveyi was revealed by MFLP-PFGE analysis. DNA fragment of V. harveyi was digested by NotI enzyme.

POTENCY AND EFFICACY TEST OF A VACCINE IN ADDITION WITH ADJUVANT AGAINST KOI HERPESVIRUS IN KOI (Cyprinus carpio) Lili Sholichah; Munti Yuhana; Angela Mariana Lusiastuti; Tri Heru Prihadi
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Koi Herpesvirus (KHV) is a malignant virus infecting the goldfish and koi in all stadia and cause mortality up to 95%. The purpose of this study was to determine the potency and efficacy of inactivated-vaccine in addition with adjuvant against KHV in koi fish. The viral propagation was done using a KF-1 cell line in 25 cm3 flask. The cultured virus was harvested on 12 days post inoculation, and then the harvested virus was inactivated with 0.1% formalin as inactivated-vaccine. Three hundred of test fish (10.38 ± 1.25 g) maintained in 126 L of plastic containers with aeration, and fed with pellets twice a day. After 14 days of adaptation, the fish were divided into five treatments (A= vaccine; B= vaccine + Complete Freund’s Adjuvant; C= vaccine + Incomplete Freund’s Adjuvant; K+= positive control, and K-= negative control) and each treatment has four replicates. Vaccine was given by injecting intramuscularly of 0.1 mL per fish. All fish were challenged by injecting intramuscularly of 0.1 mL of KHV virus with concentration of 104.58 TCID50/mL after 21 days post vaccination. The results showed that the B treatment had higher (P<0.05) values of hematocrit level, lysozyme activity, and titer of antibody compared with positive control. In addition, the survival of fish in B treatment also had the highest percentages and significantly different compared to other treatments (P<0.05). The conclusion of this research was the application of inactivated KHV vaccine in 0.1% formalin with the addition of Complete Freund’s Adjuvant through the injection dose 0.1 mL fish-1 in 104.58 TCID50/mL capable to enhance the immune responses and raised the optimal protection of KHV antibody in koi fish.

MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE Rosmiati Rosmiati; Andi Parenrengi; Emma Suryati
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE) did not exhibit any antibacterial activity against V. harveyi. From the active BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that both active compounds identified as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested that marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tiger shrimp culture.

SHORT COMMUNICATION: THE EFFECT OF TANNIN SOLUTION IN PERFORMING SUCCESFULL EGG MICROINJECTION ON AFRICAN CATFISH (Clarias gariepinus) Muhammad Hunaina Fariduddin Ath-thar; Komar Sumantadinata; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In order to successfully perform a gene transfer activity using microinjection, an individual egg is needed. The common standard operating procedure to collect the individual egg is by applying chemical solution to remove the sticky layer covering the egg surface. Among several available solutions, tannin is one of alternative substances that can be used in performing egg microinjection on African catfish. In this study, the effect of different dosages of tannin solution to remove the sticky layer of eggs was observed. The study was conducted at the Laboratory of Fish Breeding and Genetics, Bogor Agricultural University. Prior to microinjection, the eggs were treated with different dosages of tannin solution right after fertilization. There were four different levels of dosage used as the treatments i.e.: 0.3, 0.5, 0.7, and 0.9 ppm. After the treatments, each individual egg was injected using DNA pmâactin-hrGFP plasmid with a concentration of 20 μg/mL. The results showed that the dosage of 0.5 ppm of tannin was the most effective treatment compared to the other dosages. This dosage produced 71.4% hatching rate and 57.1% transgenic embryos respectively.

PATTERN OF LIPID AND ESSENTIAL FATTY ACID CHANGES IN EARLY DEVELOPMENT OF TIGER GROUPER, Epinephelus fuscoguttatus LARVAE Muhammad Marzuqi; Ketut Suwirya; Nyoman Adiasmara Giri
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Development of early stage of larvae depends on body energy content. One of energy source in early larval stage is lipid. Lipid in eggs and early larval development stage of tiger grouper (Epinephelus fuscoguttatus) is used for energy and to maintain permeability of membrane cells. Therefore, lipid class for energy decreases and lipid class for maintaining membrane increases. Lipid quality in larvae depends on the content of essential fatty acid. Lipid from larval stage of neurula; 0, 1, 3, 4, 7, 12, 15, 17, and 20 days after hatching were observed on non polar, polar lipid, and essential fatty acid. The result of experiment showed that proportion of non polar lipid from neurula phase to three days larvae (D3) decreased from 81.9% to 36.5% and polar lipid increased from 18.1% to 63.5%. This result showed, that non polar lipid is used for energy and polar lipid for maintaining membrane of cell body. Essential fatty acid, such as EPA and DHA also decreased in non polar and polar lipid. Decreasing essential fatty acids was higher in non polar lipid than these in polar lipid.

HIGH DENSITY JUVENILE SHRIMP (Penaeus monodon) PRODUCTION WITH DIFFERENT DURATIONS OF REARING IN ACID SULFATE SOIL-AFFECTED POND Akhmad Mustafa; Jesmond Sammut
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

An experiment was conducted to evaluate the effectiveness of producing juvenile shrimp in a reservoir pond constructed in acid sulphate soils (ASS). Many ponds constructed in ASS pose a significant economic risk due to high mortality and poor growth. In Indonesia, abandonment of ponds in ASS is widespread and alternative farming practices, such as production of juvenile shrimp, offer farms opportunities to lower their production risks. The experiment involved rearing juvenile shrimp at a high stocking density (3,000 individuals/m2) using hapas in a formerly unproductive pond located in Luwu Regency, South Sulawesi, Indonesia. The treatments tested included rearing periods of three and six weeks with four replications. A survival of 83.62% was achieved after rearing for three weeks compared to 45.44% after six weeks. Although there was a sixfold increase in the mean weight of shrimp from three to six weeks of culture, size variation after six weeks was large due to the high density of shrimp. The experiment showed that old acid sulphate soil-affected ponds, in which the bottom soil has not been excavated any further, can be used for production of juvenile shrimp in hapas. Rearing over three weeks is recommended to obtain high survival and reduce size variation.

REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY Sulaeman Sulaeman; Ravi Fotedar
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

FINE STRUCTURAL ANALYSIS OF UNPRODUCTIVE AND LOWPRODUCTIVE CELLS RESULTING ON CULTURED RSIV (IN VITRO) Ketut Mahardika; Teruo Miyazaki
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Red seabream iridovirus (RSIV) has been known to induce enlarged cells and inclusion body bearing cells (IBCs) allowing virus particles to propagate within viral assembly site (VAS) in the cell cytoplasm. The aim of this study was to evaluate fine structural analysis of unproductive and low-productive cells resulting on RSIV-infected cultured grunt fin (GF) cells. GF cells were treated with semi purified RSIV, and incubated for 6-14 days post cultured. The cellular enlargement were harvested, processed, and analysis under electron microscopy. Electron microscopy revealed four types of cells that were productive enlarged cells and productive IBCs which were allowing propagation of virus particles within its cytoplasm, and unproductive enlarged cells and IBCs without virus particles. Most of them were unproductive enlarged cells (80,71%-98,20%). Unproductive enlarged cell had a nucleus with enlarged cytoplasm without production of VAS with virus propagation. While, unproductive IBC had an inclusion body that was delimited from the host-cell cytoplasm by a thin membranous boundary, and was developed without virus propagation. On the other hand, lowproductive enlarged cells and IBCs contained a few number of virus particles or tubule-like structures. Therefore, the number of low-productive enlarged cells and IBCs were only a few (about 0%-14% from a total of percent productive enlarged cells and IBCs), these cells were classified into types of productive enlarged cells and IBCs. These results sugested that the unproductive and low-productive enlarged cells and IBCs were the results of RSIV-infected GF cells which failed to produce virus particles due to incapacity of RSIV virus it self and or the ability of GF cells to inhibit virus multiplication within VAS.

Page 18 of 34 | Total Record : 334

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Contact Name
Ofan Bosman

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Ofan Bosman

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Journal Mail Official
iaj.puslitbangkan@gmail.com

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Kota adm. jakarta pusat,

Dki jakarta

INDONESIA