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Teknologi Indonesia

JTI
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 01261533 EISSN : 23031913 DOI : -

Core Subject : Science,

Computer Science & IT

JTI is a journal in the Departement of Engineering Sciences - Indonesian Institute of Sciences (LIPI). JTI has policy to publish a new and original research paper or a review paper in The scope of Technology. JTI publishes two issues per year. The journal has been registered with printed-ISSN number as 0126-1533 and electronic ISSN number as 2303-1913.

Arjuna Subject : -

Articles 163 Documents

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PURIFICATION AND PROPERTIES OF MANNANASE FROM Aspergillus ustus BL5 Thontowi, Ahmad; Rahmani, Nanik; Andriani, Ade; Yopi, -
Teknologi Indonesia Vol 37, No 1 (2014)
Publisher : LIPI Press

Strain of BL5 was reported as a mannanase producer. The purposes of this study are identification, purification and characterization of mannanase from BL5. The Internal Transcribed Spacer (ITS) regions analysis showed that BL5 strain have 93% similarity with Aspergillus ustus isolate UOA/HCPF 9236. An extracellular mannanase from the culture supernatant of a fungus A. ustus BL5 was purified. SDS-PAGE of the purified enzyme showed a single protein band of molecular mass 50 to 51 kDa. The mannanase exhibited optimum catalytic activity at pH 7.0 and 55C. The metal ions Ca2+, Cu2+ and SDS inhibited complete enzyme activity. The metal ion Mg2+ and EDTA increased complete enzyme activity. The value of Vmax = 5,88 ?mol mannose/min/ml and Kmax = 0.64 mg/ml. Mannanase of A. ustus BL5 be able to hydrolyzed porang mannan.

STRUCTURE AND HARDNESS CHARACTERISTICS OF 50Cr-50Al COATING PREPARED BY A MECHANICAL ALLOYING TECHNIQUE: EFFECTS OF HEAT TREATMENT TEMPERATURE Sudiro, Toto; Sebayang, Perdamean
Teknologi Indonesia Vol 38, No 3 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i3.247

In the present work, a mechanical alloying technique was used to prepare 50Cr-50Al (in atomic, at %) coating on the surface of low carbon steel. The coated samples were then annealed in a vacuum at elevated temperatures of 600, 700, 800, and 900C for 2h, separately. The effects of annealing temperature on the structure characteristic of 50Cr-50Al coating were studied by XRD and SEM-EDX, and coating hardness was measured by micro-Vickers hardness test. The results showed that the annealing process led to the formation of inter-metallic phases and interdiffusion of Fe element from the substrate and Cr, Al elements from the coating, depending on annealing temperature. As annealing temperature increased to 900C, however, X-ray diffraction analysis indicated the formation of Al2O3. Meanwhile, the hardness of cross-sectional of coating increased after annealing at 600C. The coating hardness decreased as annealing temperatures increased.

NEURAL NETWORK TRAINING USING SEQUENTIAL EXTENDED KALMAN FILTER FOR RELIABLE ROAD FRICTION COEFFICIENT ESTIMATION Soetraprawata, Demi; Turnip, Arjon
Teknologi Indonesia Vol 33, No 2 (2010)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v33i2.26

The aim of this study is to estimate the vehicle dynamic parameters concerning with road safety (such as, road tire forces, longitudinal and lateral velocities, angular velocity, rolling radius of wheels, side slip, pitch and roll angle, and road friction coeffi cient which are diffi cult to be measured directly in a standard car) using neural network training on the basis of sequential extended Kalman fi lter (SEKF) and the recursive least squares (RLS). For such estimation, a fourteen degree-of-freedom (DOF) nonlinear full-vehicle dynamics model was developed to provide the simulation requirement. The simulation was performed and compared with CarSim (the interpreter for vehicle dynamics) to verify the model, which confi rms the expected results were all the state variables follow the CarSim response well. The simulation results show that the system performs reliably and fastly in estimating the parameters on different road surfaces during various vehicle manoeuvres.

VARIATION IN POINT MUTATIONS OF L -ARABINOSE ISOMERASE Thermotoga thermarum (TTAI) THROUGH THE ANALYSIS OF ENZYME STRUCTURE AND DOCKING SIMULATION Syahputra, Gita; Kusharyoto, Wien
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

D-tagatose is one of the products of L-arabinose isomerase which can be applied to food, cosmetics, and health. D-tagatose produced by L-AI has a low quantity. L-AI enzyme from thermophilic bacteria as Thermotoga thermarum (TTAI) can produce higher quantity of D-tagatose than mesophilic bacteria. Point mutation can increase L-AI activity. The analysis of enzyme structure and docking simulation can determine the variation in point mutations. Based on analysis of enzyme structure and docking simulation, TTAI has 494 amino acids compiled by 17 of α-helix dan 18 of β-strand. The analysis of TTAI structure get several active side residues Gln16, Leu18, Tyr19, Phe81, Gln125, His126,Met183, Phe273, Glu300, Glu327, Tyr329, His344, Met345, Ile366, His442, His443. The docking simulation suggested that the residues for binding D-tagatose are Trp422 and Tyr331. Two point mutations, i.e. M183A and F273L, are recommended based on the analysis of TTAI structure, homology structure, residues, and docking simulation. This variation in mutation can be used for further research in vitro.

IMPROVEMENT OF SOY SAUCE INOCULUM QUALITY USING A MIXED CULTURE OF ASPERGILLUS ORYZAE TG1 AND MICROCOCCUS LUTEUS Pudjiraharti, S.
Teknologi Indonesia Vol 32, No 1 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i1.54

The aim of this research is to improve the quality of soy sauce inoculum by increasing the activity of enzymes containing in inoculum which is needed for the degradation of soybean nutrition compounds. A mixed culture of Aspergillus oryzae TG1 and Micrococcus luteus with various ratio of colony number were used for inoculum preparation.The ratio of A.oryzae TG1 to M. luteus were 1:0, 1:0.25, 1: 0.50, 1:0.66, and 1:1. Incubation was done at 30 oC for four days. Inoculum with the highest enzyme activity was applied for koji preparation. The result of experiment showed that a mixed culture with ratio 1:0.25 indicated a positive effect on inoculum quality by resulting the highest activity of all enzymes tested. The Activity of ?-amylase enzym was 2055.1 Units/g, protease was 17.1 Units/g, and ?-glucosidase was 18.3 Units/g. They were observed at four days incubation. Total plate count analysis of this inoculums resulted 1.14 x 1011 cfu of A. oryzae and 2.05 x 109 cfu of M. luteus per g inoculum which were higher than that of starter. Application of the inoculums with ratio of 1:0.25 for koji preparation showed the increase of all enzymes activities in koji compared to the control inoculum (1:0).

DETERMINATION OF RETENTION INDICES OF ISOPROPYL OLEATE, ISOBUTYL OLEATE, AND DIETHYL-HEXYL PHTHALATE BY USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY Pusfitasari, Eka Dian; Andreas, -
Teknologi Indonesia Vol 36, No 2 (2013)
Publisher : LIPI Press

Diethyl-hexyl phthalate (DEHP) is the most commonly use of phthalate in plastic packed. There were several cases which subtituted the composition of DEHP in the plastic packed with vegetable oil derivative, such asIsopropyl oleate and Isobutyl oleate from palm oil, because DEHP have negative effect for health. Although it has been substituted with the Isopropyl oleate (IPO) and Isobutyl oleate (IBO), to a certain degree, the DEHP could be evaporated from the foil to its surrounding. Therefore, the analysis of both IPO and IBO is very important to be done. Analysis of IPO, IBO, and DEHP were done using GC equipment with DB-5 column and MS detector. Identifi cation by using Kovats Retention Indices (RI) values is believed to provide optimal results in qualitative analysis, since each compound has a different RI value. Determination of RI value of IPO, IBO and DEHP in various parameters using GC-MS instrument at several ramp temperature conditions of GCs column was done. Meanwhile, the variation of temperature and fl ow rate of carrier gas also became parameter to reach different RT to calculate RI. Determination of the retention indices of IPO, IBO, and DEHP showed that RI laid in the near value range. The RIvalues of those three compounds are 2194, 2317, and 2534, respectively. The different retention time of a certain compounds as a result of a shift or changes of testing parameters will not infl uence the value of its retention indices.

ANTIBACTERIAL SCREENING OF THE CULTURE OF ENDOPHYTIC FUNGAL EXTRACTS ISOLATED FROM CINNAMON STICK (Cinnamomum burmannii [Nees & T. Nees] Blume) Praptiwi, -; Ilyas, Muhammad; Wulansari, Dewi; Agusta, Andria
Teknologi Indonesia Vol 38, No 1 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i1.159

Pathogenic bacterial resistance to antibacterial agents used in the treatment of recent formal systems have undergone significant increase. It is necessary to get a source of new antibacterial agents to overcome resistance. Endophytic fungi have been known as one of the organisms that have the ability to produce a variety of bioactive metabolites. In this study, 26 endophytic fungi have been isolated from various parts of Cinnamomum burmannii plant. The results of antibacterial activity screening of endophytic fungal extracts byTLC bioautography method without elution showed that as many as 18 extracts inhibited the growth of Staphylococcus aureus InaCC-B4 with the diameter of inhibition zone (DIZ) were in the range of 5.00 to 16.00 mm. On the other hand, the number of extracts that could inhibit the growth of Escherichia coli InaCC-B5 were 24 extracts with the DIZ were in the range of 4.00 to 28.00 mm. A detailed analysis of eluted TLC-bioautography method showed the chemical compounds of extracts that were responsible for antibacterial activity.

INFLUENCE OF TAPIOCA FLOUR CONCENTRATION AND LEGUMES VARIETIES ON CHARACTERISTIC OF BLACK ONCOM WITH Mucor sp AS INOCULUM Iskandar, Yetti Mulyati; Priatni, Sri; Kardono, L.B.S.
Teknologi Indonesia Vol 33, No 1 (2010)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v33i1.17

Oncom is a product prepared by fermenting cooked peanut presscake as substrate mixed with tapioca fl our using Mucor sp as inoculum. The aim of this research is to determine the chemical composition and nutritional contents of black oncom using lupine (Lupinus angustifolius) and pigeon pea (Cajanus cajan) as substrate instead peanuts ( Arachis hypogaea ) to evaluate its potential as a new source of protein. The moisture content, pH, texture, solidness, hardness, mold growth and colour of black oncom fermented product were observed. Various tapioca fl our concentration in amount of 5%, 20% and 35% respectively were mixed on peanuts, lupine and pigeon pea as substrates. The fermentation process was conducted for 48 hours at 30oC.The result revealed that the addition 20% of tapioca to substrate showed the best performance of black oncom product and had good sensory characteristics such as texture, taste, aroma and colour based on the sensory evaluation test.

IMPROVING THE QUALITY OF BREAD USING CASSAVA FLOUR SUBSTITUTION AND α-AMYLASE khusniati, tatik; Purnamasari, Elisa; Effendi, Supli; Anindyawati, Trisanti
Teknologi Indonesia Vol 39, No 3 (2016)
Publisher : LIPI Press

Bread in Indonesia generally used wheat flour as a basic material and it is an imported flour product up to now. On the other hand, cassava flour can be used as wheat flour alternative for substitution. This research was aimed to improve the quality of bread using cassava flour substitution and α-amylase. Variables used were wheat and cassava flour with comparison: 100:0, 95:5, 90:10, 85:15, and α-amylase with concentration: 0, 0.5, 1.0, 1.5%, respectively. The contents of water, ash, protein, carbohydrate, and salt were determinated by modifying the AOAC method. The pH of bread dough was measured and α-amylase activities were determined by Iodine method. The dough and substituted-bread volumes were measured, and organoleptic tests were conducted by training 20 panelists. Statistical analysis used factorial completely randomized experimental design (FCRD). The results show that the highest values of water, ash, protein and carbohydrate were found in bread of A4B2 (25.13%), A1B1 (0.97%), A1B2 (8.62%), A4B2 (47.89%), sequentially (P<0.05). A2B4 was bread with the best organoleptic value and biggest volume than the others with nutritional contents of water (25.10%), ash (0.87%), protein (7.98%), carbohydrate (46.89%), and salt (2.17%). The values of these nutritional contents was in scope of bread SNI standard. Cassava flour substitution and α-amylase affected significantly to organoleptic values of bread produced (P<0.05). The α-amylase activities of all treated bread were not significantly different (P<0.05). Based on volumes of bread dough and bread as well as organoleptic tests, the best bread is A2B4 (95% wheat flour using 5% cassava flour and 1.5% α-amylase).

SEQUENTIAL EXTENDED KALMAN FILTER ON EEG EXTRACTION AND CLASSIFICATION Turnip, Arjon; Soetraprawata, Demi; Hariyadi, -; Kusumandari, Dwi Esti
Teknologi Indonesia Vol 36, No 1 (2013)
Publisher : LIPI Press

In this paper, a neural networks training based on Sequential Extended Kalman Filtering (SEKF) analysis for extraction and classifi cation of recorded EEG signal is proposed to improved feature extraction, classifi cation accuracy,and communication rate as well. The robustness of the SEKF against background noises has been evaluated by comparing the separation performance indices of the SEKF with well known algorithms (i.e., BPNN, JADE,and SOBI). A statistically signifi cant improvement was achieved with respect to the rates provided by raw data.

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