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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue "Vol 1, No 4 (2014)" : 2 Documents clear
DETEKSI KERAGAMAN SPESIES BAKTERI METANOGEN RUMEN SAPI MENGGUNAKAN KLONING GEN 16S rRNA DAN SEKUENSING Shoffiana Noor; Hendro Pramono; Saefuddin Aziz
Scripta Biologica Vol 1, No 4 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.925 KB) | DOI: 10.20884/1.sb.2014.1.4.43

Abstract

Ruminants produce methane gas which contributes to enhanced greenhouse effect in the atmosphere. Cattle issued the highest methane during the fermentation of feed in the rumen. Methane gas produced by methanogen bacteria in carbohydrates anaerobic fermentation. Methanogen bacteria are difficult to obtain diversity information because difficult cultured. One technique can be used is molecular rRNA 16S gene cloning and sequencing. This study was aims to determine the species diversity of methanogen bacteria in cattle's rumen using rRNA 16S gene cloning and sequencing technique by survey method. The results obtained 51 clones with 800 bp insert size length. The sequencing resulted 2 different sequences, ie 8-3L21 clone bacterium uncultured and BBS-12 clone methanogens rumen uncultured rRNA 16S gene partial sequence with 99% and 100% similarity. The Genus sequences for gene 1 and 3 were Prevotella (24%), Clostridium (1.5%), and other uncultured bacteria, whereas the 2 gene sequences of species was Methanobrevibacter ruminantium (21.83%), M. millerae (29.17%), M. gottschalkii (6.47%), Methanosphaera stadtmanae, and Methanobacterium alcaliphilum. This research provides scientific information about cattle rumen methanogen bacteria species diversity which can be used as a basis for control of cattle rumen methanogen bacteria. 
RESPON AKTIVITAS AMILASE DAN PROTEASE IKAN GURAMI Osphronemus gouramy Lac. TERHADAP PERBEDAAN TEMPERATUR AIR Dwiyan Oktavianto; Untung Susilo; Slamet Priyanto
Scripta Biologica Vol 1, No 4 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.598 KB) | DOI: 10.20884/1.sb.2014.1.4.45

Abstract

Research to determine the activity of amylase and protease digestion gourami on various temperature has been carried out experimentally using a completely randomized design (CRD) with 3 treatments and 4 replications. The treatments were included gourami nurturing on water temperature 24oC (P1), gourami nurturing on water temperature 28oC (P2), and gourami nurturing on water temperature 32oC (P3). The results the amylase activity is showed no significant difference between treatments (P>0,05) where as protease activity showed there are significant differences between treatments (P0≤0,05). In conclusion, the difference in protease activity of gourami occured in water temperature 24oC (P1) and 28oC (P2) and the protease activity was highest at 28oC water temperature, but the amylase activity did not difference.

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