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Serambi Biologi
ISSN : 23548371     EISSN : -     DOI : -
Serambi Biologi adalah sebuah jurnal yang mempublikasikan hasil penelitian ilmiah, short communication dan artikel review. Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus. Serambi Biologi dipublikasi oleh Universitas Negeri Padang dimana setiap tahunnya akan terbit sebanyak 4 kali. Penerbitan pada jurnal ini tidak dikenakan biaya.
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Articles 45 Documents
Test the Quality of the Flavor of Melinjo Seeds (Gnetum gnemon L.) Fermented Results Using Tempe Yeast Samsuriani Siregar; Dezi Handayani; Dwi Hilda Putri
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5630RF00

Abstract

Biji melinjo memiliki kandungan protein yang tinggi yang dapat dijadikan sebagai bahan baku untuk difermentasi menggunakan ragi tempe. Penelitian ini bertujuan untuk mengetahui penilaian responden terhadap biji melinjo hasil fermentasi menggunakan ragi tempe. Uji ini berguna untuk mengetahui daya terima biji melinjo hasil fermentasi di masyarakat. Parameter yang digunakan untuk uji kualitas rasa yaitu dari segi warna, tekstur, aroma dan rasa. Metode yang digunakan untuk uji organoleptik adalah metode angket. Penentuan kualitas rasa, menggunakan 20 responden yang terdiri dari 10 orang penyuka melinjo dan 10 orang bukan penyuka melinjo. Metode tersebut diuji berdasarkan warna, tekstur, aroma dan rasa biji melinjo hasil fermentasi  yang dihasilkan. Data uji organoleptik diperoleh melalui angket uji hedonik (uji kesukaan) dengan menggunakan skala likert. Data uji organoleptik diolah secara deskriptif dan disajikan dalam bentuk persentase. Hasil angket yang didapatkan sesuai dengan harapan bahwa dengan fermentasi biji melinjo, maka orang yang awalnya tidak suka menjadi lebih menerima hasil fermentasi biji melinjo. Hasil persentase bagi orang bukan penyuka biji melinjo yaitu warna  62%  (cukup suka), dari tekstur 68% (cukup suka), aroma 60% (cukup suka), dan rasa 40% (tidak suka),  sedangkan untuk orang penyuka melinjo dari segi warna 80% (suka), tekstur 78% (suka), aroma 78% (suka) dan rasa 82% (suka). Berdasarkan hasil angket yang didapatkan, dapat disimpulkan bahwa biji melinjo hasil fermentasi menggunakan ragi tempe bisa diterima oleh responden.  Key word: Melinjo, Fermentasi, Kualitas Rasa
Effect of Giving Fermented Liquid Areca Cathecu L. and Surian Leaves (Toona sinensis ROXB.) On Tilapia Wounds (Oreochromis niloticus L.) Audina Mutia
Serambi Biologi Vol 3 (2018): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/4251RF00

Abstract

Tilapia (Oreochromis niloticus L.) is a species originating from the Nile River region and surrounding lakes in Africa. Maintenance of tilapia is also at risk for diseases and pests. Diseases in fish require fast and effective handling, namely by administering drugs. The use of safe, environmentally friendly, and low-cost drugs is herbal medicine, which is using areca leaf fermentation liquid (Arecha cathecu L.) and surian leaves (Toona sinensis ROXB.) This research is an experimental study, using a completely randomized design (CRD) consisting from 4 treatments and 6 replications with 7 days of observation. Data were analyzed using Analysis Of Variants (ANOVA), the results were significantly different followed by continued testing of DNMRT level of 5%. Based on the research conducted, it is known that the dosage of Areca cathecu L. and surona sinensis ROXB. Fermented leaves which has the most effect on wound healing in tilapia (Oreochromis niloticus L.) which has the longest wound healing is 0.54 cm in A3 treatment (administration of areca leaf fermented liquid and surian leaves with a dose of 15 ml while the lowest healing was at 0 ml (control).
Bioaugmentation Effect of Bacillus sp. and Pseudomonas sp. Isolates on Lowering Used Lubricating Oil- Contaminated Soil pH Mutia Dwi Herakasih; Yuni Ahda
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5171RF00

Abstract

The purpose of this study was to look at the effect of bioengineering on the pH value of used oil contaminated soil. This research was conducted from February to April 2018. This study used Pseudomonas sp. Isolates. and Bacillus sp. from previous research by Ahda and Fitri (2016). The bacterial formulations are Pseudomonas sp, Bacillus sp, and consortium. The number of biaoaugmentation used in this study was 6 times the fermentation. Calculation of pH values is done once a week and carried out for 42 days of the study period. The results of this study indicate that bioaugmentation affects the pH value of used oil contaminated soil.
ANTAGONIST TEST OF PSEUDOMONAD FLUORESEN WHICH WAS GROWED IN SOME TYPES OF CARBON SOURCES AGAINST BLOOD DISEASE BACTERIA Kurnia Anggraini
Serambi Biologi Vol 3 (2018): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/4234RF00

Abstract

. Fluorescent pseudomonads are biocontrol agents that can control several plant diseases. Plant diseases can be caused by bacteria, fungi and nematodes. Blood Disease Bacteria (BDB) is a bacterium that causes blood disease in banana plants. Blood Disease Bacteria (BDB) disease control can be done using biocontrol agent Cas3 fluorescent pseudomonad isolates. This research is an experimental study, using a completely randomized design (CRD) with three treatments and five replications. The treatment is 1. glucose, 2. fructose and 3. glycerol. The data observed were inhibitory zones formed after testing the flourescent pseudomonad against Blood Disease Bacteria (BDB). Data were analyzed using the Analysis of Variance (ANOVA) test and continued with a DNMRT further test at the level of 5%. The results obtained are different carbon sources affecting the size of the inhibitory zone formed by the Cas3 flourescent pseudomonad against BDB. Meanwhile, the best carbon source for the growth of flourescent pseudomonads in producing a inhibitory zone against BDB.
Test the Quality of Taste of Jengkol Seeds (Pithecellobium jiringan (Jack) Prain.) Fermented Results Novita Novenra; Dezi Handayani
Serambi Biologi Vol 4 (2019): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5632RF00

Abstract

Biji Jengkol memiliki kandungan protein yang tinggi yang dapat dijadikan bahan baku untuk difermentasi menggunakan ragi tempe.  Penelitian ini bertujuan untuk mengetahui pendapat responden terhadap kualitas rasa tempe biji jengkol hasil fermentasi menggunakan ragi tempe. Uji ini berguna untuk mengetahui daya terima hasil fermentasi biji jengkol dimasyarakat. parameter yang digunakan dalam penelitian  ini untuk uji kualitas rasa pada hasil fermentasi biji jengkol yaitu warna, tekstur, aroma, dan rasa. Metode yang digunakan untuk uji organoleptik adalah metode angket yaitu angket uji hedonik (uji kesukaan). Uji ini melibatkan 20 orang panelis yang terdiri dari 10 orang penyuka biji jengkol dan 10 orang bukan penyuka biji jengkol. Uji tersebut diuji berdasarkan warna, tekstur, aroma, rasa hasil fermentasi biji jengkol yang hasilkan. Data uji organoleptik diperoleh melalui angket uji hedonik (uji kesukaan). Data uji organoleptik diolah secara deskripsif dan disajikan dalam bentuk persentase,. Berdasarkan hasil persentase yang diperoleh dari penyuka biji jengkol didapatkan hasil warna 78% (suka), tekstur 80% (suka), aroma 76% (suka), rasa 78% (suka), sedangkan yang bukan penyuka jengkol didapatkan hasil warna 76% (suka), tekstur 80% (suka), aroma 38% (tidak suka), 26% (tidak suka). Hasil analisa menunjukkan bahwa hasil fermentasi menggunkan ragi tempe dapat diterima oleh responden dengan kriteria memiliki bau yang normal (khas tempe), warna putih, tekstur kompak serta rasa yang normal (tidak terasa asing). Key word: Biji jengkol, Fermentasi, Kualitas Rasa  
THE EFFECT OF GIVING FERMENTATION FLOWS OF PINANG LEAF (Areca catechu L.) AND SURIAN LEAF (Toona sinensis ROXB.) ON CUPANG FISH WASH (Betta splendens Regan.) seva miranti
Serambi Biologi Vol 3 (2018): Bio Sains
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/4266RF00

Abstract

Betta fish (Betta splendens Regan.) Is an ornamental fish that has high economic value because of the beauty of color and its fighting instincts. . However, fans of ornamental fish prefer male fish than females because male fish have better and more attractive aesthetic and color values and have higher profits. There are two non-parasitic diseases that commonly attack Betta fish, namely bite the tail and bruising. During this time the treatment used always uses chemicals such as PK solutions, methylene blue and melachite green. The use of chemicals does not always have a positive effect but can also have a negative impact on non-targeted organisms. Another effort taken to treat this disease is to use natural ingredients, namely by using fermented liquids from areca leaves and surian leaves. Medicinal plants or plants can be obtained by fermentation. Areca leaves and surian leaves are capable of producing secretions of secretive drugs. The test results of organic compounds of fermented liquid areca leaves and surian leaves containing antibiotics and other organic compounds that work synergistically accelerate healing and relieve pain or pain due to injury to the culture. This study was an experimental study, using a factorial complete randomized design consisting of two factors, namely the dosing factor (A) and the length of the observation day (B). Each treatment was repeated 6 times. Data were analyzed using Analysis Of Variants (ANOVA), the results were significantly different followed by continued testing of DNMRT level of 5%. Based on the research conducted, it can be concluded that the administration of fermented areca leaves (Areca catechu L.) and surian leaves (Toona sinensis ROXB.) Affects wound healing in Betta fish (Betta splendens Regan.) And the fastest treatment to help wound healing process that is on treatment A3 with a dose of 15 ml. 
Bioaugmentation Effect of Alcaligenes sp.2 and Isolates Bacillus sp.2 on Lowering Used Lubricating Oil- Contaminated Soil pH Nur Afni; Yuni Ahda
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5793RF00

Abstract

Abstract. This study aims to see the effect of biugmentation on pH values on contaminated oil used soil. In this study using Alcaligenes sp.2 isolates. and Bacillus sp.2 which was successfully isolated by Ahda and Fitri (2016). The bacterial formulation is a single isolate of Alcaligenes sp.2, Bacillus sp.2 and the Alcaligenes sp.2 and Bacillus sp.2 consortium. The variation in frequency of biaoaugmentation used in this study was 6 times biotechnology. Calculation of pH values is done once a week and carried out during the 6 weeks of the study period. The results of this study indicate that bioaugmentation influences the pH value of contaminated oil used soil. The type of bacteria that is the fastest in reducing and increasing pH in contaminated oil from used oil is a consortium of Alcaligenes sp.2 bacteria. and Bacillus sp.2.Keywords: Bioaugmentation, Hydrocarbons, pH, Alcaligenes sp.2. and Bacillus sp.2.
The Difference Chlorophyll of Wheat Grass (Triticum aestivum L.) Planted with Different Media riska nur hayati; Elsa Yuniarti
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5798RF00

Abstract

Planting media is a provider of nutrients that play an important role in increasing chlorophyll levels in plants. Wheat grass is one of the high chlorophyll plants and is beneficial for health. The purpose of this research was to determine the differences in the chlorophyll content of wheat grass grown with soil and charcoal media (hidroponik). The research was an experimental which was analyzed by the One Sample T-test at a significant level of 5%, conducted in February 2019 at the Biology Research Laboratory of the FMIPA UNP. Measuring chlorophyll levels using the Wintermans method with a leaf sample of 100 g which is 10 days old after planting. The results showed that there was a significant difference in the chlorophyll content of wheat grass which was planted with soil and charcoal media (hydroponic) media because of Sig. (2-tailed) which is 0,030<0,05 and the highest chlorophyll content is found in wheat grass planted with soil media.
PRECISION ENUMERATION OF THE NUMBER OF BACTERIAL CELLS WITH THE SPREAD PLATE METHOD USING DILUTION Elsa Alfiyanti; Dwi Hilda Putri
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5768RF00

Abstract

Bakteri endofit isolat B.J.T.A.2.1 merupakan isolat yang menghasilkan senyawa antimikroba yang baik. Tetapi, saat ini belum diketahui persisi jumlah sel bakteri tersebut. Penelitian ini bertujuan untuk mengetahui dan mendapatkan persisi jumlah sel bakteri. Penelitian ini dilakukan dengan cara enumerasi melalui perhitungan menggunakan pengenceran. Metode penanaman yang digunakan adalah metode sebar (spread plate). Metode penelitian yang digunakan adalah deskriptif yaitu melakukan analisis data dan disajikan dalam bentuk grafik dari jumlah sel bakteri. Parameter analisis adalah jumlah sel bakteri. Hasil penelitian menunjukkan bahwa persisi jumlah sel bakteri adalah -0,8 hingga +1,2. Zona biru pada grafik adalah persisi (ketepatan) jumlah sel bakteri.kata kunci: persisi, jumlah sel bakteri
GROWTH CURVE OF ENDOPHYTE BACTERIA ANDALAS PLANT (Morus macroura Miq.) B.J.T A-6 ISOLATE Mahjani Mahjani; Dwi Hilda Putri
Serambi Biologi Vol 5, No 1 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5692RF00

Abstract

Laju pertumbuhan bakteri sangat bervariasi menurut jenis bakteri serta kondisi lingkungannya. Setiap bakteri memiliki waktu yang berbeda pada masing-masing fase pertumbuhannya. Laju pertumbuhan dapat ditentukan dengan cara membuat kurva pertumbuhan antara waktu inkubasi dengan absorban yang dihasilkan. Penelitian ini bertujuan untuk untuk mempelajari dan mendapatkan kurva pertumbuhan bakteri endofit Andalas isolat B.J.T A-6. Penelitian ini merupakan penelitian deskriptif yaitu membuat kurva pertumbuhan dengan cara menghubungkan waktu fermentasi dengan absorban yang dihasilkan. Hasil penelitian menunjukkan bahwa pertumbuhan isolat B.J.T A-6 terdiri dari 4 fase, yaitu fase adaptasi, logaritmik, stasioner, dan kematian. Fase adaptasi terjadi pada jam ke-1. Fase logaritmik terjadi pada jam ke 2 sampai jam ke 7 ,fase stasioner terjadi pada waktu 4-5 jam. Fase kematian terjadi pada jam ke 20-24