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Serambi Biologi
Published by Universitas Negeri Padang
ISSN : 23548371     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary
Serambi Biologi adalah sebuah jurnal yang mempublikasikan hasil penelitian ilmiah, short communication dan artikel review. Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus. Serambi Biologi dipublikasi oleh Universitas Negeri Padang dimana setiap tahunnya akan terbit sebanyak 4 kali. Penerbitan pada jurnal ini tidak dikenakan biaya.
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Articles 45 Documents
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Potential Fluorescent Pseudomonad Cas Isolate that Growed in Various Formulas to Produce IAA Nola Nurdianata; Linda Advinda
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/10339RF00

Abstract

The purpose of this research was to determine the potential of fluorescent pseudomonad with Cas isolates grown in various formulas to produce Indole Asetic Acid (IAA). This experiment was conducted from June to July 2020, at the Plant Physiology Laboratory of FMIPA, UNP. In this research, IAA detection was carried out by qualitative and quantitative analysis. The research was use descriptive method with fluorescent pseudomonad isolate Cas and treatment consisting of M1 (molasses 10 g/L+ ZA 5 g/L), M2 (molasses 10 g/L+ ZA 10 g/L), M3 (molasses 5 g/L+ ZA 5 g/L), M4 (molasses 5 g/L+ ZA 10 g/L), and M5 (NB 8 g/L). The results showed that the fluorescent pseudomonad of Cas isolate was able to produce IAA. This can be seen in the pink color produced after addition of Salkowsky's reagent and incubation for 12 hours in a dark room. Then, the fluorescent pseudomonad of Cas isolate grown in the M1 formula had the highest IAA concentration of 37.295 ppm, while the lowest IAA concentration was in the fluorescent pseudomonad isolate Cas formula M3 with an IAA concentration of 2.897 ppm.
THE EFFECT OF TEMPERATURE ON THE ACTIVITIES OF AMOBIL XILANASE ENZYMES IN THE PAPER BIOBLEACHING PROCESS (PULP) Irdawati Irdawati; Fauzana Ahmad; Syamsuardi Syamsuardi; Agustien Agustien; Y Rilda
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/6023RF00

Abstract

Abstrak. Enzim xilanase merupakan enzim extraseluler yang mampu menghidrolisis xilan menjadi xilosa. Isolat SSA 2 merupakan isolat dari bakteri termofilik hasil isolasi dari air panas sapan sungai aro, yang memiliki kemampuan menghasilkan enzim xilanase. penggunaan xilanase merupakan salah satu alternative mengurangi pemakain clorin dalam proses pemutihan pulp. xilanase diamobilisasi dengan menggunkan NA alginate. pulp difermentasi dengan suhu 50ºC, 55ºC, 60ºC, 65ºC, 70 ºC, 75 ºC, 80ºC, selama 6 jam, suhu optimum yang menghasilkan bilangan kappa terendah dan aktivitas enzim yang tertinggi pada suhu 60ºC.
Potensi Bacillus sp. dalam Menghasilkan Indole Acetic Acid (IAA) serta Pengaruhnya Terhadap Panjang Akar Kecambah Benih Cabai Merah (Capsicum annuum L.) Yulanda Saputri; Linda Advinda; Moralita Chatri; Dezi Handayani
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/9545RF00

Abstract

Indole Acetic Acid (IAA) is a natural form of the auxin hormone found in plant roots and can stimulate plant growth. IAA functions to regulate physiological processes and stimulate plant growth such as cell lengthening and enlargement. Bacillus sp. including Plant Growth Promoting Rhizobacteria (PGPR) whose role is to increase the germination rate of red chili seeds (Capsicum annuum L.). This study aims to determine the ability of Bacillus sp. against the length of the root sprouts of red chili seeds (Capsicum annuum L.). This research was conducted in in August until November 2019 at the Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Padang. The method used is descriptive and quantitative experiments. The results obtained showed that the isolate Bacillus sp. able to produce IAA and administration of isolates Bacillus sp. significant effect on root length of the red chili sprouts. Keywords : Indole Acetic Acid (IAA), Bacillus sp.
The Effect of Hydrocarbonoclastic Bacteria Bioaugmentation (Bacillus sp. & Alcaligenes sp.) toward Soil pH Marcloryne Agnesia; Yuni Ahda
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/6032RF00

Abstract

The used lubricant oil is one of the petroleum derivates wich contains an alkane group with 17-22 carbon atoms. In general, petroleum waste has not been managed optimally. One of the effective methods of combating petroleum waste is by involving hydrocarbonoclastic bacteria. The purpose of this research is to determine the effect of hydrocarbonoclastic bacteria bioaugmentation on soil pH values. This research was conducted in June until December 2018. This study used a single indigenous bacterium and consortium (Bacillus sp. and Alcaligenes sp.) which was isolated from contaminated soil by Ahda and Fitri (2016). pH measurements were carried out on the 1st 2nd 3th, 4th, 5th and 6th week. The results showed that the amount of bioaugmentation affected the soil pH value in the third week, and the type of bacteria began to affect the soil pH in the sixth week. The results of this study indicate that bioaugmentation affects the pH value of used oil contaminated soil.
THE ANTIMICROBIAL ACTIVITY OF DMSO AS A NATURAL EXTRACT SOLVENT Miftahul Rahmi; Dwi Hilda Putri
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/5909RF00

Abstract

Abstrak. Salah satu penyebab meningkatnya kasus infeksi karena adanya peningkatan jumlah kuman yang resisten terhadap agen antimikroba. Isolat B.J.T.A.2.1 merupakan bakteri endofit dari tanaman Andalas (Morus macroura Miq.) yang diketahui mampu menghasilkan senyawa aktif antimikroba. Penelitian ini bertujuan untuk mengetahui pengaruh pelarut DMSO yang merupakan pelarut dari  ekstrak etanol produk fermentasi terhadap hasil uji. Uji aktivitas pelarut dilakukan dengan metode difusi kertas cakram. Hasil penelitian menunjukkan bahwa tidak ada pengaruh pelarut DMSO terhadap hasil uji. Kata kunci: ekstrak etanol produk fermentasi, senyawa antimikroba, DMSO.  Abstract. One of the causes of increased cases of infection is the increase in the number of germs that are resistant to antimicrobial agents. Isolate B.J.T.A.2.1 is an endophytic bacterium from Andalas (Morus macroura Miq.) Plant which is known to produce antimicrobial active compounds. This study aims to determine the effect of DMSO solvent which is a solvent from ethanol extract of fermented products on the test results. The solvent activity test was carried out by paper diffusion method. The results showed that there was no effect of DMSO solvent on the test results. Keywords: ethanol extract of fermented products, antimicrobial compounds, DMSO.
Chlorophyll Ratio of Kale (Ipomea reptans Poir.) Which Are Cultivation With Hydroponick And Non Hydroponick Sintia Delia Syafitri; Resti Fevria
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11082RF00

Abstract

Chlorophyll is a pigment that gives a green color to the leaves and stems of a plant. Chlorophyll plays an important role in the process of photosynthesis, besides chlorophyll also contains high antioxidants, anti-inflammatory properties and is a wound healing agent. Chlorophyll is found in green vegetables, especially kale. The leaves and stems of kale contain lots of vitamins, protein, calcium, phosphorus, sitosterol and minerals, especially iron which are useful for the growth and health of the human body. Water spinach has many benefits in overcoming constipation, preventing anemia, preventing insomnia and other benefits. Water spinach can be grown hydroponically and non-hydroponically. The objectives of this study were: to determine the chlorophyll content of kale plants cultivated hydroponically and non-hydroponically. This research was conducted at the Wire House of the Biology Laboratory of Padang State University in January-March 2020. This research is a descriptive study. The analysis was carried out: chlorophyll content of kale. Hydroponic kale samples were obtained from the West Sumatra Hydroponic Community in Alai Padang, while the non-hydroponic samples were purchased at Pasar Raya Padang, the analysis of vitamin C content was carried out by the Spectrophotometric Method. From the research that has been done, it is found that the average content of chlorophyll content in hydroponic cultivated kale is 12.21 mg/L and non-hydroponic 12.34 mg/L. The conclusion of the research results is that the chlorophyll content of kale cultivated by hydroponics is lower than that of water spinach cultivated non-hydroponically.
Primer Design and in Silico PCR for Detection Shigella Sp. on Refilled Water Samples Deratih Bunga Purwakasih
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11072RF00

Abstract

Refillable drinking water is an effort to meet the needs of drinking water for consumption by modern society. One of the quality parameters of drinking water suitable for consumption is not contaminated with Escherichia coli, Salmonella sp. and Shigella sp. Conventional microbiological tests were often conducted for the detection of water pathogenic bacteria, including culturing the bacteria in medium culture and biochemical assay, but this method requires a long working time and expensive costs. Currently, Polymerase Chain Reactions (PCR) can be an alternative method because it has high accuracy. Primers are one of the important components of PCR so they must be specifically designed to ensure the success of DNA amplification. This study aimed to obtain the specific sequence of PCR primer for detection Shigella-contaminated refillable drinking water sample and conducted the in silico PCR. Primers were designed in silico using Primer BLAST in NCBI (National Center for Biotechnology Information) and Geneious Prime for in silico PCR. To get a Shigella-specific primer, pairwise alignment of Shigella sp. (NC_004337.2) and E.coli (NC_000913.3) was performed and specific sequence of Shigella was used as primer candidates. The result of this study were the Shigella-specific sequence PCR primer forward 5'-GCTAATGAAAATGGCGCTGT-3' and reverse 5'-AGCCGACGGTTTGAAGTTAC-3' with PCR product length of 815 bp.
Profil Reproduksi Kucing Betina Ras Persia (Fellis catus) di Kecamatan Air Manjuto, Kabupaten Mukomuko, Bengkulu Faiza Octaviana; Ramadhan Sumarmin
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11136RF00

Abstract

Abstract.  The purpose of this study to determine the reproductive profile of female Persian cats in Air Manjuto District, Mukomuko Regency, Bengkulu. This research is a descriptive study conducted using a visual encountered survey method directly into the field to determine the reproductive profile of female Persian cats and the data is presented in the form of a percentage in the table. This research was conducted from September to Desember 2020 in Air Manjuto District, Mukomuko Regency, Bengkulu. Calculation of the ratio of male and female kitten is done by calculating the sex ratio. The results showed that the reproductive time of Persian female cat started at the age of 9 months to 10 months and the average reproductive time of the cat was 9.67 months. Based on the length of gestation, Persian female cat can reach for 60-65 days and the average cat's pregnancy is 62.67 days. The average number of kittens born to these cats was 4.67. Based on the ratio of male and female kitten, the most female sex was compared to male sex with a ratio of 42.9% male and 57.1% female. And the overall it can conclude that the ratio of birth dominate by female kittens.Key words: Persian breed female cat, reproductive profil. Abstrak.  Tujuan penelitian ini untuk mengetahui profil reproduksi kucing Persia betina di Kecamatan Air Manjuto Kabupaten Mukomuko Bengkulu. Penelitian ini merupakan penelitian deskriptif yang dilakukan dengan metode visual encountered survey langsung ke kelapangan untuk mengetahui profil reproduksi kucing persia betina dan datanya disajikan dalam bentuk persentase pada tabel. Penelitian ini dilaksanakan pada bulan September sampai Desember 2020 di Kecamatan Air Manjuto, Kabupaten Mukomuko, Bengkulu. Perhitungan rasio anak kucing jantan dan betina dilakukan dengan menghitung perbandingan jenis kelamin. Hasil penelitian menunjukkan bahwa waktu reproduksi kucing persia betina dimulai pada umur 9 bulan sampai dengan 10 bulan dan rata-rata waktu reproduksi kucing tersebut adalah 9,67 bulan. Berdasarkan lamanya masa kebuntingan, kucing persia betina dapat mencapai 60-65 hari dan rata-rata masa kebuntingan 62,67 hari. Jumlah rata-rata anak kucing yang lahir adalah 4,67. Berdasarkan rasio jenis kelamin anak kucing jantan dan betina, jenis kelamin paling banyak berjenis kelamin betina dibandingkan dengan jenis kelamin jantan dengan perbandingan 42,9% jantan dan 57,1% betina. Dan secara keseluruhan dapat disimpulkan bahwa rasio kelahiran didominasi oleh anak kucing betina.Kata kunci: Kucing Betina Ras Persia, Profil Reproduksi
Effect of Rambutan Leaf Extract (Nephelium lappaceum L.) on Colony Diameter and Percentage of Growth of Inhibition Fusarium oxysporum Fauziahtul Azmi
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11732RF00

Abstract

Abstract. Wilt disease is caused by the fungus Fusarium oxysporum. This fungus is difficult to eradicate and can survive in the soil for a long time. This disease causes considerable damage to plants. To control this disease, many farmers use synthetic fungicides. The continuous use of synthetic fungicides is not good for humans and the environment. To overcome this problem, plant-derived pesticides are used. Nephelium lappaceum L. (Rambutan) is a plant that has the potential as a vegetable pesticide.leaf extract N. lappaceum contains several antifungal compounds, namely saponins, tannins, and flavonoids. The purpose of this study was to examine the effect of leaf extract concentration of N. lappaceum on colony diameter and the percentage of inhibition of growth of F. oxysporum. This research was carried out from February 2021 to April 2021, at the Integrated Research Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with the administration of leaf extract at N. lappaceum concentrations of 0%, 10%, 20%, 30%, 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (DNMRT) further test.. The results showed that the leaf extract of N. lappaceum. effect on the growth of fungal colonies of F. oxysporum. Each treatment was significantly different from the control and each treatment showed significant differences from each other. The concentration of leaf extract affected the percentage of growth inhibition of F.oxysporum. The lowest percentage of inhibition was at a concentration of 10% and the highest was at a concentration of 40%.Abstrak. Penyakit layu fusarium disebabkan oleh jamur Fusarium oxysporum. Jamur ini sulit dibasmi dan dapat bertahan di tanah untuk waktu yang lama. Penyakit ini menyebabkan kerusakan  yang cukup besar pada tanaman. Untuk  mengendalikan penyakit ini petani banyak menggunakan fungisida sintetik. Penggunaan fungisida sintetik secara terus menerus berdampak tidak baik bagi manusia dan lingkungan. Untuk mengatasi masalah tersebut maka digunakan pestisida nabati yang berasal dari tumbuhan. Nephelium lappaceum L. (Rambutan) merupakan salah satu tanaman yang berpotensi sebagai pestisida nabati. Ekstrak daun N. lappaceum mengandung beberapa senyawa yang bersifat antifungi yaitu saponin, tanin, dan flavonoid. Tujuan penelitian ini  untuk  melihat pengaruh konsentrasi ekstrak daun N. lappaceum terhadap diameter koloni dan persentase penghambatan pertumbuhan F. oxysporum. Penelitian ini dilaksanakan pada bulan Februari 2021 hingga April 2021, bertempat di Laboratorium Penelitian Terpadu Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang. Penelitian ini merupakan penelitian eksperimen yang terdiri dari 5 perlakuan dan 3 ulangan dengan pemberian ekstrak daun N. lappaceum konsentrasi 0%, 10%, 20%, 30%, 40%. Data yang diperoleh dianalisis dengan sidik ragam (ANOVA) dengan uji lanjut Duncan’s New Multiple Range Test (DNMRT). Hasil penelitian menunjukkan bahwa ekstrak daun N. lappaceum. berpengaruh terhadap diameter koloni jamur F. oxysporum. Pada setiap perlakuan berbeda nyata dengan kontrol dan masing-masing perlakuan menunjukkan perbedaan yang nyata satu sama lain. Konsentrasi ekstrak daun berpengaruh terhadap persentase perhambatan pertumbuhan F.oxysporum. Persentase penghambatan terendah pada konsentrasi 10%   dan paling tinggi pada konsentrasi 40%.
Inventarisasi Tumbuhan Obat Berpotensi Antidiabetes di Kecamaran IV Koto Kabupaten Agam Nurul Yanifa; Syamsurizal Syamsurizal
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11717RF00

Abstract

Pengobatan Tradisional untuk dibetes mellitus (DM) masih digunakan oleh masyarakat di Kecamatan IV Koto Kabupaten Agam, terutama pengobatan jangka panjang diabetes mellitus. Penelitian ini dilakukan karena potensi tanaman obat khususnya diabetes mellitus di Kecamatan IV Koto Kabupaten Agam masih belum terdata dengan baik. Penelitian ini bertujuan untuk mengetahui jenis-jenis tanaman obat antidiabetes, bagian organ tanaman yang digunakan dan cara pengolahan tumbuhan obat. Penelitian dilakukan dengan menggunakan metode survei dan wawancara. Hasil penelitian ditemukan 42 species dari 32 familia tanaman obat yang diduga sebagai pengobatan diabetes mellitus. Tumbuhan yang paling banyak ditemukan adalah familia Asteraceae. Terdapat 32 jenis tanaman obat yang digunakan secara tunggal dalam pengobatan dan 10 jenis tanaman obat yang digunakan dalam bentuk kombinasi atau ramuan. Organ tumbuhan yang digunakan adalah daun, akar, umbi, rimpang, kulit dan batang. Pengolahan tanaman obat untuk pengobatan diabetes mellitus adalah dengan cara direbus, ditumbuk, diperas dan dikeringkan.

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