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Journal of Stem Cell Research and Tissue Engineering

JSCRTE
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Published by Universitas Airlangga

ISSN : 26141264 EISSN : 26141256 DOI : https://dx.doi.org/10.20473/jscrte

Core Subject : Health, Science, Engineering,

Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.

Arjuna Subject : Kedokteran - Imonologi dan Alergi

Articles 85 Documents

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Histomorphology of Pancreatic Islet in Physiological Aging Female Rats Post Intravenous Human Wharton’s Jelly Mesenchymal Stem Cell Injection wining astini
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The increasing population of aged people will have the important role in the life, but the function of their bodies will decrease because of aging. Aging will increase the risk of degenerative disease, one of example is diabetes. The disease is related to the aging in the pancreatic organ which progressively declines by age. The aimed of the experiment was to determine the effect of human wharton’s jelly mesenchymal stem cells by injecting intravenously in aging female rats. This study used 3 young female rats (3 months) and 6 aging female rats (24 months). The experiment consisted of three groups. The young control group (A), the aging control group (B) that received NaCl (0.9%) 0,4 mL, the aging treatment group (C) received 1 x 106 cells/kg of human wharton’s jelly mesenchymal stem cells 0,4 mL. The aging control and the aging treatment group were injected 4 times with the interval in 3 months. The end of the experiment (12 months), the rats were anesthetized and sacrificed. The pancreatic tissues were collected to examine the pancreatic islets by histology studies. Changes of the pancreatic islet in control and treated groups were examined using hematoxylin and eosin staining. These findings conclude that injecting human wharton’s jelly mesenchymal stem cell increase the diameter and total pancreatic islet in the treatment group. In other side, the cell population of pancreatic islet also have significant differences (P<0.05) in treated physiological aging female rat groups than control aging female rat group.

THE EFFECT OF INJECTION OF INTRA ARTICULAR ALLOGENIC BONE MARROW MESENCHYMAL STEM CELL-PLATELET CELL RICH PLASMA (BMSCs-PRP) ON FULL-THICKNESS ARTICULAR CARTILAGE DEFFECT REGENERATION IN RABBIT Taufik, S. Ahmad
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 1 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The joint cartilage defectfullthickness is still a problem today because its currenttreatment still has not delivered maximum results. Current treatment uses cartilage enginering using mesenchymal stem cells alone and or combining growth factor. The aim of this study was to investigate the effect of intra-articular injection of Allogenic bone marrow mesenchymal stem cell - Platelet rich plasma (BMSCs-PRP) on regeneration of cartilage defect fullthickness in rabbits. The design of this study was a post-test only control group design using 36 New Zealand white rabbits divided into three groups. Each group were treated with PRP, BMSCs and BMSCs-PRP. Results were evaluated after 10 weeks. In the evaluation, macroscopic images showed the best healing in the BMSCs-PRP group. Histopathologic examination showed that in the MSCs-PRP group, there was a significant increase in the number of chondrocytes (p = 0,000), cartilage area (p = 0,000), as well as the number of Agecoprogenitorexpec- tion cells (p = 0,000) and collagen type 2 (p = 0,000). BMSCs were able to differentiate into condroblasts which then synthesize aggressive and collagen type 2. Platelet rich plasma (PRP) contains growth factor BMP, TGF, FGF and IGF which can accelerate the occurrence of MSCs differentiation. Intra-articular injections Allogenic bone marrow mesenchymal stem cell (BMSCs-PRP) is able to regenerate and cure full-thickness joint cartilage defects through differentiation of MSCs into condroblasts.Keyword: Allogenic, Bone marrow Mesenchymal stem cell, Cartilage, Platelet rich plasma, Full-thickness.

Potential Hollowfiber Polyurethane-Collagen of Chitosan Coatings As a Nerve Graft for the Therapy of Peripheral Nerve Injuries in Limb Paralysis Maulida, Hendita
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Peripheral nerve injury with a gap of 5–30 mm can cause permanent paralysis because it causes an axon to break up. The distance between axons of more than 1-2 cm requires a graft in the form of a nerve connector to fix it. Synthesis of chitosan coated polyurethane-collagen hollowfiber has been carried out as an accelerator for healing peripheral nerve injury. The results of Fourier Transform Infra Red (FTIR) analysis showed a cross link between chitosan and glutaraldehyde seen in the shift of wave numbers from 1080-1100 cm-1 to 1002 cm-1. The degradation test results showed that the sample experienced a decrease in mass after being immersed in Simulated Body Fluid (SBF) for 7 days. Polyurethane can be degraded in the body after 30 days. This is in accordance with the mechanism of the nerve which regenerates 1 mm per day or 1 inch per month. Scanning Electron Microscope (SEM) analysis showed that the diameter of the hollowfiber was 2.021-2.032 mm which corresponds to the peripheral nerve diameter of 1.5-3 mm and the pore size of the wall is 31.33-39.65 Î¼m. The results of this study are expected to provide the theoretical basis for the use of chitosan polyurethane-collagen coating composites as nerve grafts for the treatment of peripheral nerve injuries that have biocompatible properties, can regenerate and are easily degraded and provide alternative solutions for nerve graft needs that are more economical and easier to manufacture so widely produced in Indonesia.

Desensitization with Self-Antigen dsDNA Inhibits B and T-cell Functions by Modulating Treg as Regulator Immune System in Pristane-induced Lupus Mice Model saiful arifin
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The aim of this study was to develop a novel therapeutic method for improving immune system regulation in SLE using escalating dose self-antigen dsDNA immunotherapy. Methods: Female Balb/c mice were given a single intraperitoneal injection of 0.5 ml pristane.. Starting at 12 weeks after injection,. the mice were evaluated for clinical and serological manifestations. Mice with lupus signs (PIL mice) were divided into four groups; positive control group, PIL A (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml ) EDI dsDNA, PIL B (0.1 μg/ml, 1 μg/ml, 10 μg/ml ) EDI dsDNA, and PIL C(1 μg/ml, 10 μg/ml, 100 μg/ml). EDI dsDNA were administered once every week in consecutively. The doses would increase every week. dsDNA were complexed with the cationic polyethylenimine (PEI) before injection. Samples were analyzed for autoantibodies levels (dsDNA, ANA) and ,TGF-β cytokine from serum using ELISA and T-Reg, mature dendritic cells from spleen using flowcytometry.. Results: Escalating dose antigen spesific immunotherapy with self-antigen dsDNA significantly decreased ANA (p=0.02), anti-dsDNA (p=0.03), dendritic cell mature (p=0.02) compare to positive control, and not significantly decreases Th17 cells (p=0,18) but the result tend to get lower. Desensitization using self-antigen dsDNA was increased T-reg proliferation (p=0.00) and level of TGF-β (p=0.03) significantly compare to positive control. Conclusion: Desensitization using self-antigen dsDNA coupled to PEI was able to modulate T-Reg as a regulator immune respon and inhibit B and T cell functions in lupus mice model.

INFLUENCE OF LOW OXYGEN CONDITION OF BONE MARROW MESENCHYMAL STEM CELL setyowardoyo, nugroho
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 1 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

The state of low oxygen levels known as hypoxia in humans is considered a dangerous condition is apparently a normal physiological condition and required by the stem cells as they are in the body. Mesencyimal Stem Cells (MSCs) require physiologically optimal conditions of low O2 tension of 1-3% in the bone marrow. The purpose of this study was to reveal the difference between in vitro culture of MSC in normoxia condition (20% O 2 concentration) with hypoxia condition (1% O2 content) especially in terms of viability, pluripotent properties, and MSC proliferation ability of the culture it produces. This research is an explorative laboratory research invitro on Bone Marrow Mesenchymal Stem Cells (BMSCs) culture using hypoxia condition. The study design used Randomized Control Group Post-Test Only Design. This research was conducted for 2 months. There was a significant difference in mean slow proliferation based on the number of Least-like CFU-Cs between the control group, treatment group 1 and treatment group 2, the mean percentage of the number of cells expressing the OCT4 coding gene on immunofluorosense examination between the control group, 1 and the treatment group 2 and the mean percentage of cell numbers expressing the OCT4 coding gene on the immunofluorosense examination between the control group, the treatment group 1 and the treatment group 2 showing p <0.01. There was a significant difference of percentage of non-absorbing color cell number of trypsin blue (viable cells) between control group, treatment group 1, and treatment group 2 showing p value <0.05. This suggests that the precondition of culture with normoxia provides an opportunity for cells to adapt and proliferate before being conditioned in hypoxic cultures. Cultures with hypoxic conditions and preconditions of normoxia are the best culture conditions because they produce cells that are capable of maintaining pluripotency properties while still having better proliferation and viability capability compared with direct hypoxia conditions.Keywords: Hypoxia, normoxia, bone marrow, mesenchymal stem cells.

The Effect Of Immersion Time Variation in Polyvynyl Piprolidone Against Characteristics Of Scaffold Biocomposit Of Bacterial-Hydrocysiatatic Cellulose as Candidate indrio, ludita
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Bone defects due to trauma, tumors, congenital abnormalities, degeneration and other diseases are still major problems in the field of orthopedics and traumatology. Based on data in Asia, Indonesia is the country with the highest number of fracture sufferers, there are as many as 300-400 cases of bone surgery per month in hospitals. Dr. Soetomo Surabaya (Gunawarman et al, 2010). Repair of damaged bones can be overcome with material that can accelerate the process of bone healing (bone healing). This research was conducted to synthesize hydroxyaparite bacterial cellulose scaffold as a candidate for bone healing. Bacterial cellulose as a matrix was synthesized by culturing Acetobacter xylnum, while hydroxyapatite as filler was synthesized by immersion into a solution of CaCl2 and Na2HPO4, the scaffold formation process using freeze dried method. Composite formation was varied by immersion in Polyvynil pirrolidone (PVP) for 0, 1, 2, 3, 4 days. Furthermore, samples were characterized using FTIR-Spectroscopy showing the presence of carbonates containing apatite crystals in all five samples.

THE EFFECT OF ALLOGENIC FREEZE DRIED PLATELET-RICH PLASMA IN RESPONSES INFLAMMATION REACTION OF RABBIT rachmawati, trio
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

This study aims to analyze the effects of allogenic freeze dried platelet-rich plasma in responses inflammation reaction of rabbit. The designs of this study are one group pretest posttest conducted to determine the effect of freeze drying on levels of TGF-β1 PRP and post test only control group design conducted to determine the effect of allogenic freeze dried PRP. Nine samples of PRP which examined levels of TGF-β1 before and after freeze drying were obtained from blood centrifugation of three rabbits. These nine samples were used as allogenic donor which injected intramuscularly in nine rabbits for the treatment groups. The control group used nine rabbits which was injected intramuscularly using autologous PRP. Both groups were observed inflammatory response. Measurement of TGF-β1 levels before and after freeze drying were tested statistically using T- test dependent. Data inflammatory response were tested statistically using T- test independent. The results showed that no effect of freeze drying process on levels of TGF-β1. Allogenic freeze dried PRP did not cause an iflammatory response.Keywords : autologous, allogenic, freeze dried platelet rich plasma, transforming growth factor- β1.

Multiple Injection of Human Wharton’s Jelly Mesenchymal Stem Cells Ameliorate Spermatogenic Cells on Physiological Aging Male Rats alif imam fitrianto
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 1 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Recently, the most common therapy on men who suffered fertility decline due to aging was testosterone replacement, but now it is known that this therapy has a long-term risk of damage to the cardiovascular system and prostate. Stem cells are an alternative therapy that has a potency to improve the fertility of aging men that less causing side effects. The aim of this study was to evaluate the injection of Human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSC) in physiologic aging male rats on spermatogenic cells. This study used 3 young male rats (8-12 weeks) and 6 physiological aging rats (22-24 months) which divided into 3 groups, the young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and physiological aging male rats received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on histological analysis. The results indicate that the hWJ-MSCs injections increased the number of spermatogonia and Leydig cells significantly (P<0.05), and improve the tubules circumference and interstitial area significantly (P<0.05). The mechanism of spermatogenic cells repairs suspected due to various bioactive molecules that secreted by hWJ-MSCs which can affect the surrounding cells.

COLLAGEN ANALYSIS OF GRAFT IN BONE TUNNEL MODEL ANTERIOR CRUCIATE LIGAMENT (ACL) RECONSTRUCTION WITH INTRATUNNEL ALLOGENIC BONE MARROW MESENCHYMAL STEM CELLS (MSCs) AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) petrasama, petrasama
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 1 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

ACL reconstruction measures aim to obtain stable knees, and reduce the risk of further injury to the meniscus and joint surfaces. Acceleration of the integration process between the tendon graft and the bone tunnel will improve the final result of ACL reconstruction. The purpose of this study was to investigate the collagen composition of the bone tunnel graft model of anterior cruciate ligament reconstruction with intravenous allogenic bone marrow mesenchymal stem cells and vascular endothelial growth factor in experimental animals. The design of this study was Post-test only Control Group Design using 20 rabbits divided into treatment group and control group. Collagen immunohistochemical evaluation was performed at weeks 3 and 6. Evaluation at week 3 obtained the area of collagen type-1 in the higher treatment group at treatment (p <0.001). In the 6th week evaluation, it was found that the area of collagen type-1 in the treatment group was higher (p <0.05). Type-1 collagen at week 6 did not differ significantly with week 3 (p> 0.05). Provision of allogenic bone marrow mesenchymal stem cells and intratonal vascular endothelial growth factor in ACL reconstruction enhanced the formation of collagen type-1 which is the acceleration of incorporation of the graft tendon process with bone tunnel.Keywords : Anterior Cruciate Ligament, allogenic bone marrow mesenchymal stemcells, vascular endothelial growth factor, graft and collagen.

Injectable Bone Substitute Paste Based on Hydroxyapatite, Gelatin and Streptomycin for Spinal Tuberculosis hendita maulida
Journal of Stem Cell Research and Tissue Engineering Vol. 3 No. 2 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v3i2.20133

World Health Organization (WHO) in 2005 reported that cases of tuberculosis (TB) in the world occur more than 8 million annually and 5-10% were attacked in spine. The most effective treatment of spinal TB is evacuation of infected bone segments and fill with bone graft. It has been synthesized and characterized of injectable bone substitute (IBS) paste based on hydroxyapatite, gelatin and streptomycin. IBS paste synthesized by mixing hydroxyapatite and gelatin 20% w/v with 75:25, 70:30, 65:35 and 60:40 ratio and streptomycin 10 wt%. The mixture was then added with hydroxypropyl methylcellulose (HPMC) 4% w/v as suspending agent. In vitro characterization performed include acidity (pH), injectability test, setting time, cytotoxicity (MTT assay) and microbacterium test. Acidity test results indicate a fourth variation of the samples had pH values approaching normal body pH (7,3 to 7,6) and is able to maintain stability when measured in 7 days. Injectability test results indicate IBS paste is injectable with the highest percentage of the injectability value at 97,74% ± 0,19%. IBS paste has been setting within 30 minutes to 1 hour when injected on hydroxyapatite scaffold that resembles the bone cavity and is able to cover the pore scaffold seen from the Scanning Electron Microscope (SEM). Scaffold pore size is smaller from range of 780,8 to 835,4 μm into 225,2 μm. MTT assay results showed that IBS paste is not toxic and experiencing proliferation (viability >100%) that are expected to trigger osteoblast cell growth when applied. Microbacterium test results showed that IBS paste is an antibacterial seen from inhibition zone diameter of Staphylococcus aureus and has a high strength-sensitive antibacterial. Thus, hydroxyapatite, gelatin and streptomycin composites had qualified as injectable bone substitute which applied in cases of spinal tuberculosis.

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Contact Name Fika Kharisyanti

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Editorial Address Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

Location Kota surabaya, Jawa timur INDONESIA

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Contact Name
Fika Kharisyanti

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fikakharisyanti@gmail.com

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+6282232687366

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Ruang Stem Cell, Gedung Lembaga Penyakit Tropis Lantai 2, Kampus C Universitas Airlangga

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Kota surabaya,

Jawa timur

INDONESIA