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Ratmawati Malaka
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Hasanuddin Journal of Animal Science (HAJAS)
Published by Universitas Hasanuddin
ISSN : 26219182     EISSN : 26219190     DOI : -
Core Subject : Economy, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Immunology & microbiology
HAJAS aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 92 Documents
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Effect of Mixed Cropping Between Zea maize and Arachis pintoi on the Nutrient Content of Grain and Straw A. N. I. Insani; M Mariam; M. Yusuf; S. Sudarsono
Hasanuddin Journal of Animal Science (HAJAS) Vol. 8 No. 1 (2026)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v8i1.48423

Abstract

ABSTRACT This study aimed to evaluate the effect of mixed cropping of maize (Zea mays) with pinto peanut (Arachis pintoi) on the nutrient content of maize grain and straw. A randomized group design (RGD) was employed with five treatments and three replications. The treatments consisted of P0 (100% maize), P1 (80% maize + 20% legume), P2 (50% maize + 50% legume), P3 (20% maize + 80% legume), and P4 (100% legume). The parameters observed included crude protein, neutral detergent fiber (NDF), and acid detergent fiber (ADF) content of maize grain and straw. Analysis of variance revealed that mixed cropping had no significant effect (P>0.05) on the nutritional composition of both maize grain and straw. In conclusion, intercropping maize with Arachis pintoi does not significantly influence the nutritional quality of maize under the conditions of this study. Keywords: Arachis pintoi, maize feed, mixed cropping, production
In Vitro Development and Gene Expression of Pig Embryos Cloned from Induced Pluripotent Stem Cells and Embryonic Fibroblasts N. S. Machebe; T. Tani; S. Shimizu; M. Hata; K. Ohata; T. Fukuda; D. Hufana-Duran; Y. Kato
Hasanuddin Journal of Animal Science (HAJAS) Vol. 8 No. 1 (2026)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v8i1.50505

Abstract

Somatic cell nuclear transfer (SCNT) in pigs remains inefficient, mainly due to incomplete nuclear reprogramming of donor cells. Induced pluripotent stem (iPS) cells have been proposed as promising nuclear donors, but their developmental competence in porcine SCNT is unclear. This study compared the in vitro developmental ability of embryos reconstructed with porcine embryonic fibroblasts (PEFs) or porcine iPS (piPS) cells. Developmental rates, total and epiblast cell numbers were assessed in SCNT, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and parthenogenetic activation (PA) embryos. The effect of oocyte activation status on piPS-derived SCNT embryos was also examined. Expression of well-known pluripotency transcription factor genes OCT4, SOX2, NANOG, c-MYC, and KLF4 were quantified by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Results showed cleavage and blastocyst formation rates of piPS-derived embryos (55.0% and 14.6%) were comparable to PEF-derived SCNT, IVF, and ICSI, while PA embryos showed significantly higher rates (67.8% and 44.5%). Non-activated oocytes yielded higher development than activated ones. Blastocyst cell numbers were similar across groups; however, SCNT embryos contained fewer epiblast cells than IVF and PA. qRT-PCR showed markedly reduced OCT4, comparable SOX2, NANOG, and c-MYC, and higher KLF4 in NT and PA embryos than IVF. The results conclude that piPS cells can serve as nuclear donors but do not improve cloning efficiency compared with fibroblasts. Aberrant lineage allocation and dysregulated gene expression suggest incomplete reprogramming, highlighting the need for improved piPS quality. Key words: induced pluripotent stem cells (iPSCs), pig, nuclear transfer

Page 10 of 10 | Total Record : 92

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