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Dian Agustin W
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cdj@journal.unair.ac.id
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+62315030255
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cdj@journal.unair.ac.id
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DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132
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Kota surabaya,
Jawa timur
INDONESIA
Conservative Dentistry Journal
Published by Universitas Airlangga
ISSN : 20871848     EISSN : 27228045     DOI : 10.20473/cdj.v10i1.2020.1-4
Core Subject : Health,
Journal of conservative dentistry accepts original manuscripts in the field of Endodontic other related subjects articles, including research, case reports and literature reviews. The spread of fields include: Endodontic research; Preventive, curative and rehabilitative related to endodontic field; Oral health education and promotion related to endodontic field; Endodontic and restorative clinical research; Basic sciences related to endodontic field; Endodontic healthcare management
Articles 10 Documents
Search results for , issue "Vol. 6 No. 2 (2016): July - December" : 10 Documents clear
Konsentrasi Efektif Ekstrak Daun Salam (Syzygium polyanthum Wight) terhadap Hambatan Biofilm Enterococcus faecalis (Effective Concentration of Bay Leaf Extract (Syzygium polyanthum Wight) to Inhibit Enterococcus faecalis Biofilm) Shufiyah Nurul Aini; Ruslan Effendy; Ira Widjiastuti
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.358 KB) | DOI: 10.20473/cdj.v6i2.2016.87-92

Abstract

Background. Enterococcus faecalis is the most dominant microorganisms found in endodontic secondary infection with prevalence ranging between 24% - 77%. Defense mechanism of Enterococcus faecalis bacteria is forming biofilm,. A study showed that bacteria in mature biofilms can 10-1000 times more resistant to antimicrobials than bacteria in a planktonic form. One of the natural substances that can be used as antibiofilm to irrigation root canals is extract of fresh bay leaf (Syzygium polyanthum Wight). Chemical components in bay leaves include flavonoids, tannins, and essential oils, which have antibacterial capability and damage the membrane biofilm. Purpose. To determine the effective concentration of fresh bay leaf extract (Syzygium polyanthum Wight) that can inhibit biofilm Enterococcus faecalis. Method. This research is in-vitro labolatory experimental with post test only control group design using microtitter plate assay. Samples using Enterococcus faecalis ATCC 29212 cultured in TSB (Trypticase Soy Broth) + glucose.Bay leaf extract (Syzygium polyanthum Wight) concentration in this study was 13%, 12.25%, 11.50%, 10.75%, 10%, 9.25%, 8.50%, 7.75%, 7%, and 6.25%. Results. At the 13% concentration of Syzygium polyanthum Wight, showed 100% inhibition of biofilm, means that the 13% concentration of bay leaf extract (Syzygium polyanthum Wight) can totally inhibit biofilm formation of Enterococcus faecalis. Conclusion. The effective concentration of bay leaf extract (Syzygium polyanthum Wight) which inhibit Enterococcus faecalis biofilm is 13%.
PREVALENSI KARIES GIGI POSTERIOR BERDASARKAN KEDALAMAN, USIA DAN JENIS KELAMIN DI RSGM FKG UNAIR TAHUN 2014 (Prevalence of Posterior Teeth Caries by The Depth of Cavity, Age and Gender at RSGM FKG UNAIR in 2014) Amelia Kristanti Rahardjo Rahardjo; Ira Widjiastuti; Edhie Arif Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.72 KB) | DOI: 10.20473/cdj.v6i2.2016.66-70

Abstract

 Background. Dental caries is one of the most prevalent chronic disease of people worldwide, and also Indonesia. Based on Indonesian Basic Research from  Health Department, prevalence of dental caries in Indonesia was 90.5%. When dental caries rates are reported by gender, female are found to exhibit higher prevalence rates than male. Dental caries is disease of lifetime because it can spread out more throughout life and will continue until the teeth are destroyed without a proper treatment.  Purpose. The aim of this study is to analyze the prevalence of posterior teeth caries by the depth of cavity, age, and gender at RSGM FKG UNAIR.  Method. Analytic observational study with cross-sectional and stratified random sampling method. Result. Out of 420 patients with teeth caries, 169 male patients (40.2 %) and 251 female patients (59.8 %). The highest prevalence is deep caries (59.76%) and the lowest prevalence is superficial caries (15.95%). Conclusion. It was concluded that there is  correlation between the depth of cavity increase by age and female has higher caries prevalence than male but no significance differences between cavity depth and gender. 
Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis) Ranggi Hardian Nugro Astuti; Karlina Samadi; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.762 KB) | DOI: 10.20473/cdj.v6i2.2016.93-98

Abstract

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml  Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.
Perbedaan Efektivitas Daya Antibakteri Ekstrak Daun Mimba (Azadirachta Indica A. Juss) Dibanding NaOCl 2,5% Terhadap Enterococcus Faecalis (The Difference of Antibacterial Effectiveness of Neem Leaf Extract (Azadirachta Indica A. Juss) Than NaOCl 2,5% Against Enterococcus Faecalis) Setian Fitri Sayekti; Agus Subiwahyudi; Edhie Arif Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.449 KB) | DOI: 10.20473/cdj.v6i2.2016.71-76

Abstract

Background. Enterococcus faecalis is often found in persistent endodontic infection. Its high prevalence, about 24-77%, is due to resistance and virulence of these bacteria. NaOCl is commonly use as irrigation material, but it has toxicity effect and can irritate periapical tissues. Therefore, some studies to find natural materials that have antibacterial properties as an alternative root canal medicament need to be done. Neem (Azadirachta Indica A. Juss), also known as wonder tree for centuries has antibacterial properties, proven in the preliminary study which has been done that the MIC (Minimum Inhibitory Concentration) is 65% and MBC (Minimum Bactericidal Concentration) is 70%. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of the Neem leaf extract (Azadirachta Indica A. Juss) than NaOCl 2,5% against Enterococcus faecalis. Methods. This study is an experimental labolatory with post test only control group design usingEnterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of 65% Neem leaf ectract, 70% Neem leaf ectract and NaOCl 2,5% by measuring the inhibition zone diameter of each treatment.Result. Diameter of bacterial inhibition zone formed Neem leaf extract is greater than NaOCl 2,5%. Conclusion. The antibacterial activity of Neem extract is greater than NaOCl 2,5% against Enterococcus faecalis.
Daya Antibakteri EkstrakMeniran (Phyllanthus niruri linn) Terhadap BakteriEnterococcus faecalis (Antibacterial Activity Of Phyllanthus niruri linn Extract Against Enterococcus faecalis Bacteria) Tri Desiana KH; Achmad Sudirman; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.961 KB) | DOI: 10.20473/cdj.v6i2.2016.99-104

Abstract

Background. Enterococcus faecalis is an anaerobic facultative gram-positive bacteria which contribute to the failure of root canal treatment with the number of prevalence 24% to 77%. At the preparation stage, a material for irrigation which has antibacterial activity to Enterococcus faecalis is needed. Phyllantus niruri linn is one of herbal medicament which is potential as antibacterial agent as it contains active antibacterial chemical-compound. Purpose. The purpose of the study is to identify Minimum Inhibitory Concentration and Minimum Bactericidal Concentrationof Phyllantus niruri linn against Enterococcus faecalis.Method. The research method used is laboratory experimental. Enterococcus faecalis ATCC 29212 was suspended into several concentration of Phyllantus niruri linn extract from dilution method on BHIB medium. Each tube was incubated for 24 hours. Then, each tube was subcultured to Nutrient agar medium using spreader in a petridish. Each petridish was incubated for 24 hours and the growth of the colony was manually calculated using CFU/ml unit. Result. At the concentration of 6.25%, Phyllanthus niruri linn was able to inhibit the growth of Enterococcus faecalis as 90% and there was no bacteria at the concentration of 12.5%. Conclusion. 6.25% concentration of Phyllanthus niruri linn extract was Minimum Inhibitory Concentration and 12.5% concentration was Minimum Bactericidal Concentration to Enterococcus faecalis.
Perbedaan Kebocoran Tepi antara GIC Konvensional dan Resin Modified GIC pada Restorasi Kelas V (Difference on Microleakage of Conventional GIC and Resin Modified GIC in Class V Restoration) Aditya Rama Devara; Cecilia G.J Lunardhi; Tamara Yuanita
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.276 KB) | DOI: 10.20473/cdj.v6i2.2016.77-81

Abstract

Background. Microleakage is one of the challenging concerns in direct filling restorations. Restoration material should have good adaptation between the restoration and the cavity walls to seal the cavity in a good way. Glass ionomer cement (GIC) is one of restoration material which indicated for class V cavity. GIC has a good adherence to the cavity wall but fragile to liquid contamination during the setting time. Resin modified glass ionomer cement (RMGIC) is a hybrid glass ionomer cement with the addition of hidroxyethylmethacrylate (HEMA) in the liquid to increase the properties and endurance to liquid contamination. Aim. The aim of this study was to investigate the difference on microleakage of conventional GIC and resin modified GIC in class V restoration. Material and methods. Class V cavities (depth: 2 mm) were prepared on the cervical surface of  32 human first permanent  premolars. Teeth were classified into three groups. Group 1: conventional GIC + varnish. Group 2:RMGIC + varnish. All cavities were restored, then stored in artificial saliva at 37ºC for 24 hours. The teeth were immersed in a 1% methylene blue dye solution for 24 hours, and then rinsed in running water, dried, and sectioned longitudinally. The section were assessed for microleakage of dye penetration by two independent evaluators using a digital microscope. Data were collected and statistically analyzed. Results. RMGIC showed no significant difference with conventional GIC. However, there is a slight difference, RMGIC has a slight lower microleakage than conventional GIC. Conclusion. RMGIC showed only slight lower microleakage than conventional GIC, but not significant.
Konsentrasi Efektif Daya Antibiofilm Kitosan Cangkang Udang terhadap Streptococcus Viridans (The Effective Concentration of Antibiofilms Capacity from Shrimp Shells Chitosan towards Streptococcus Viridans) Nur Ariska Nugrahani; Sri Kunarti; Laksmiari Setyowatie
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.222 KB) | DOI: 10.20473/cdj.v6i2.2016.105-109

Abstract

Background.Periapical tooth infection is one of infection problems which often happens such as abscess periapical which is caused by bacteria. The bacteria which can form biofilms is named streptococcus viridans. It is  resistant towards an antibacterial agent. Chitosan made of shrimp shells is used as a natural antibiofilms agent for streptococcus viridans. Purpose.To determine the effective concentration of antibiofilms capacity from shrimp shells chitosan towards streptococcus viridans. Method.The research method used in this research is laboratory experimental research. The research design is post-test only controlgroup design. Streptococcus viridans is given vortex until it becomes homogeneous with standard turbidity McFarland of 0.5, than, it is planted inside a microtitter plate using TSB Glu for 5x24 hours. At last, Streptococcus viridans is colored using crystal violet and the picture of biofilms is observed using inverted microscope. Chitosan liquid diluted through various concentration 0.195%, 0.39%, 0.78%, 1.56%, 3.125%, 6.25%, 12.5%, 25%, 50% and 100% are going to be added to the microtitter plate and being incubated for 24 hours. The interpretation of the result on the longitude of the wave through optical density is 570nm. Result.There is a significant difference between the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39% and the control group. Chitosan’s effective concentration in resisting the biofilms is 50%. The result is determined by statistical analysis. Conclusion.The effective concentration to resist the formation of Streptococcus viridans biofilms using shrimp shells chitosan is 50%.
Efektivitas Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi Linn) sebagai Bahan Pembersih Saluran Akar Gigi (The Effectiveness of Wuluh Starfruit (Averrhoa bilimbi Linn) Leaf Extract as Root Canal Cleaner) Amalia Dara N; Ira Widjiastuti; Laksmiari Setyowati
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.284 KB) | DOI: 10.20473/cdj.v6i2.2016.82-86

Abstract

Background: Preparation is one of the stages in endodontic treatment that always produce debris as a result of intrumentation on root canal walls. Debris consist of organic and inorganic materials. Debris will impede the penetration of disinfection materials and reduce attachment of the root canal filling material, therefore needed root canal irrigant which can remove the debris. Wuluh starfruit leaf extract (Averrhoa bilimbi Linn) contain various active compound, such as saponin. Saponin act as “surfactants” which can remove the debris. Purpose: This study was to determine the cleaning effectiveness concentration of wuluh starfruit leaf extract which can clean the root canal walls from debris. Method: Twenty five first premolar human teeth with straight single roots were randomized into five groups in which each group consist of five teeth. Those teeth instrumented with K-file and irrigated as follows: Group 1 (control) used aquadest as irrigant, group 2 used extract 5%, group 3 used extract 10%, group 4 used extract 15%, and group 5 used extract 20%. Then, those teeth were split horizontally and longitudinally in 1/3 apex. After that, the apical third of root canal walls were observed by using scanning electron microscope (SEM). Result: There were significant differences between each group (p<0,05). Group 4 and 5 had smallest median score that was 1. Saponin in wuluh star fruit leaf extract 15% has been reach critical micelle concentration (CMC). Conclusion: Wuluh starfruit leaf extract 15% effective for cleaning root canal walls from debris.
DAYA ANTIBAKTERI EKSTRAK DAUN KEDONDONG BANGKOK (Spondias dulcis Forst.) TERHADAP BAKTERI Enterococcus faecalis Antibacterial Potency of Kedondong Bangkok Leaves Extract (Spondias dulcis Forst.) against Enterococcus faecalis Bacteria Singgih Harseno; Latief Mooduto; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.929 KB) | DOI: 10.20473/cdj.v6i2.2016.110-116

Abstract

Background. The prevalence of endodontic infection after root canal treatment caused by the Enterococcus faecalis bacteria ranged between 24-77%. It is caused by resilience and virulence from Enterococcus faecalis. An alternative  solution have to be done toward irrigation of root canal wall which is effective to kill bacteria. Kedondong Bangkok (Spondias dulcis Forst.) is one of the plants or natural substance potentially as an antibacteria. The antibacterial potencies of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria could be identified by determining Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). Purpose. This study is aimed to prove antibacterial potencies by identifying Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria. Method. This study is an experimental laboratories through research design of The Post Test Only Control Group Design. Value of MIC and MBC were known by counting the growth of Enterococcus faecalis bacteria by treating the leaves extract of Kedondong Bangkok  with concentration respectively 25%, 22,5%, 20%,17,5%, 15%, and 12,5% on nutrient agar media in CFU/ml. Result. In the concentration 12,5% there are 8.8% bacterial growth and in the concentration 15% there are no bacterial growth. Conclusion. Kedondong Bangkok leaf extract (Spondias dulcis Forst.) has an antibacterial potency against Enterococcus faecalis bacteria. The MIC shows in concentration of 12,5% and the MBC shows in concentration of 15%.
DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH Rega Maurischa Arantika Putri; Tamara Yuanita; Mohammad Roelianto
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.736 KB) | DOI: 10.20473/cdj.v6i2.2016.61-65

Abstract

Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.

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