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Conservative Dentistry Journal

CDJ
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Published by Universitas Airlangga

ISSN : 20871848 EISSN : 27228045 DOI : 10.20473/cdj.v10i1.2020.1-4

Core Subject : Health,

Dentistry

Journal of conservative dentistry accepts original manuscripts in the field of Endodontic other related subjects articles, including research, case reports and literature reviews. The spread of fields include: Endodontic research; Preventive, curative and rehabilitative related to endodontic field; Oral health education and promotion related to endodontic field; Endodontic and restorative clinical research; Basic sciences related to endodontic field; Endodontic healthcare management

Arjuna Subject : Kedokteran Gigi - Kedokteran Gigi (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol. 7 No. 1 (2017): January - June" : 10 Documents clear

Hubungan Sikap Kesehatan Gigi dan Mulut Penderita Terhadap Kepatuhan dalam Menjalani Perawatan Berulang Rizkina Hendani; Edhie Arif Prasetyo; Galih Sampoerno
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Caries is the world's number one chronic disease and the prevalence increases in modern times. But most people ignore it and choice dental care only when it feels pain. It will worsen the condition of the tooth until require multivisit treatments. The number of visits required for multivisit treatments results incomplete treatment due to patient’s non-obedience. One of the factors that affect obedience is attitude. One's attitude towards something will determine someone's behavior towards the same thing. Purpose: To determine the relationship of patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. Method: Analytical observational research with cross-sectional study conducted on 30 patients who came to RSGMP UPF Conservative Dentistry FKG UNAIR Surabaya period September-November 2011. The data about the attitude is obtained from questionnaire, while collecting cards of patient’s status and direct interviews of the operators conducted to obtain data about patient’s obedience. Result: Based on the results of Spearman Correlation with α=0,05 obtained p-value=0,364 (p>0,05). It suggests that there is no significant relationship between patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. In addition, test results of the relationship between unexamined variables with obedience obtained p-value=0,010 (motivation), p=0,017 (desire), p=0,004 (perception), and p=0,009 (financing). This suggests that there is a significant relationship between motivation, desires, perceptions, and financing with obedience. Conclusion: There is no relationship between patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. However, other factors found associated, namely motivation, desires, perceptions, and financing.

Perbedaan lama perendaman air perasan jeruk nipis (Citrus aurantifolia Swingle) terhadap kekasaran permukaan resin komposit nanohybrid Faustina Angela Tandrayuana; Edhie Arif Prasetyo; S. Angela Setyabudi
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Nanohybrid Composite is a composite that is often used in dentistry because it has advantages in physical, mechanical and esthetic properties. One of the mechanical properties which can be an indicator of the resilience of restorative material is surface roughness. One of the factors that affect the surface roughness is acidic drink consumption habits. Fruit that easily processed into fresh juice and widely consumed as beverage is lime. Lime has a sour taste because it has main content of citric acid. Purpose: The aim of this study was to determine the effect of prolonged lime juice consumption to the roughness of nanohybrid composite surface. Methods: 24 composite nanohybrid samples sized 5x2 mm divided into 4 groups. The control group was immersed in sterile water, the treatment group was immersed in lime juice for 2.5 hours, 15 hours and 30 hours. Before and after immersion, all samples were tested using a Surface Roughness Tester. Data were tested statistically. Results: Kruskal Wallis Test and Tukey HSD showed significant changes in surface roughness after being immersed in lime juice. Group of 30 hours immersion obtained the greatest increase in surface roughness. Conclusion: The 30 hours immersed composite group has rougher surface than 15 hours. The 15 hours immersed composite group has rougher surface than 2,5 hours

Perbedaan daya antiglukan NaOCl 2,5% dan ekstrak kulit manggis (Garcinia mangostana L.) 0,09% terhadap Enterococcus faecalis (Comparison of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract (Garcinia mangostana Linn) 0.09% against Enterococcus faecalis) Nabiela Rahardia; M Rulianto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background. Failure of endodontic treatment is commonly caused by the persistent microorganisms remaining in the root canal such as Enterococcus faecalis. Enterococcus faecalis can form a biofilm in tough environmental conditions within the root canals and caused biofilm-mediated infections which needs more complicated treatment due to the increasing of antimicrobial resistance. The biofilm formation initial and most important step is bacteria adherence to the solid surface that is mediated by glucan. NaOCl 2.5% is a commonly used root canal medicaments but can cause injury of periapical tissue. Mangosteen pericarp extract contains flavonoid, tannin, and xanthone have mechanism for inhibiting adherence of bacterial biofilm. Difference of antibacterial activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% can be determined by experimental laboratory to determine the adherence of bacteria in each treatment. Purpose. The aim of this study was to assess the difference of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% on Enterococcus faecalis. Method. This study was designed as an experimental laboratory study with post test only control group design using Enterococcus faecalis ATCC 29212. Mangosteen pericarp was extracted using maceration method. Adherence analysis was observed after 24 hours by examining the viable cells in suspension. These viable cells are measured by UV-Vis spectrophotometer to compare the suspensions’ turbidity. Using the Independent T-Test, significantly less bacteria were found adhering to the mangosteen pericarp extract. Results. Absorbancy difference level by mangosteen pericarp extract 0.09% is significantly greater than the NaOCl 2.5% (p<0.05). Conclusion. Antiglucan activity that generated by mangosteen pericarp extract 0.09% is greater than NaOCl 2.5%

Daya Hambat Aktivitas Enzim Glukosiltransferase (Gtf) Streptococcus mutans Oleh Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb.) A. Amanda; Sri Kunarti; Agus Subiwahjudi
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Streptococcus mutans is a bacteria which has glucosyltransferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Temulawak (Curcuma xanthorrhiza Roxb.) is one of the traditional herbs which has xanthorrhizol, curcumin, flavonoid, tanin, and saponin as an antibacterial agent. Purpose: The purpose of this research is to investigate the effect of temulawak extract (Curcuma xanthorrhiza Roxb.) to the activity of GTF enzyme Streptococcus mutans. Method: This research used 25%, 37,5%, and 50% concentration of temulawak extract as the treatment, and 0.12% chlorhexidine gluconate as a control. The method of this research consists of three steps; preparing the temulawak extract concentration of 25%, 37,5%, and 50%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour. Result: The obtained data then were analyzed by Post-Hoc Tukey (HSD). The result showed a significant difference between each treatment group with the control group (p<0.05). Conclussion: This research concludes that temulawak extract with 25%, 37,5%, and 50% concentrationcan’t inhibit the GTF enzyme activity of Streptococcus mutans.

UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21 Qurni Restiani; Mandojo Rukmo; Devi Eka Juniarti
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity) Ivon Dewi Setianingrum; Ketut Suardita; Ari Subiyanto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries, and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cavity on the first group used 0,78% saponin from mangosteen pericarp extract as cavity cleanser, the second group used 6% citric acid as cavity cleanser, and the control group used aquadest. Then, the teeth were split to be observed on Scanning Electron Microscope (SEM). Result: For Mann- Whitney test there were significant differences just between 078% saponin from mangosteen pericarp extract with 6% citric acid, and 6% citric acid with aquadest, but not for 0,78% saponin from mangosteen pericarp extract with aquadest. Median value of 6% citric acid showed 2,000 which is the smallest value compared to the value of the other groups. Conclusion: The cleanliness of cavity with 6% citric acid is better than that with 0,78% saponin from mangosteen pericarp extract.

Kemampuan hambat ekstrak temulawak (Curcuma xanthorrhiza Roxb.) terhadap adhesi bakteri Streptococcus mutans Erin Imaniar B; Sri Kunarti; Widya Saraswati
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background : Streptococcus mutans is the most cariogenic microorganism in the oral cavity. Streptococcus mutans has the ability to use dietary carbohydrates, such as sucrose, to synthesize extracellular polysaccharides (glucan and fructan) through glucosyltransferase and fructosyltransferase. Extracellular polysaccharide has a role in promoting the adhesion of bacteria. Chlorhexidine is the gold standard as antiplaque agent but chlorhexidine is not fully able to inhibit the adhesion of bacteria because it has an effect that can increase gtfC and gtfD expression on S. mutans in planktonic form. Temulawak extract (Curcuma xanthorrhiza Roxb.) contains of curcumin, xanthorrhizol, saponin, flavonoid and tannin that have an effect to inhibit activity and secretion of glucosyltransferase and fructosyltransferase. Purpose: The aim of this study is to find out the ability of temulawak extract (Curcuma xanthorrhiza Roxb.) to inhibit adhesion of Streptococcus mutans. Method: This study was designed as an experimental laboratory study with post test only control group design using Streptococcus mutans. Temulawak was extracted using maceration method. The number of bacteria that can perform adhesion to the tooth surface can be determined by performing calculations using formulas plate count method. Result: Temulawak extract (Curcuma xanthorrhiza Roxb.) at concentrations of 25%, 37.5%, 50% and chlorhexidine can inhibit bacterial adhesion of Streptococcus mutans. Conclusion: Temulawak extract at concentration of 50% and 37.5% has the best ability to inhibit the adhesion of bacteria among other treatment groups.

Daya bunuh dan daya hambat antimikrobial chlorhexidine 2% dan povidone iodine 1% sebagai medikamen saluran akar terhadap Enterococcus faecalis The ability of chlorhexidine 2% and povidone iodine 1% as root canal medicaments to kill and inhibit Enterococcus faecalis Nathania Astria; Ari Subiyanto; Latief Mooduto
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background. Enterococcus faecalis is one of the pathogenic organisms associated with root canal treatment failure and apical periodontitis. Root canal medicament is needed to prevent re-infection in the root canal and increase the success of treatment. Chlorhexidine and povidone iodine is a broad spectrum root canal medicaments that can kill gram-positive bacteria. Purpose. The purpose of this study was to discover the ability to kill and inhibit of antimicrobial chlorhexidine 2% and 1% povidone iodine asvroot canal medicaments against bacteria Enterococcus faecalis. Methode. This research was done by measuring the inhibition zone and count the number of colonies. Determination of the inhibition of root canal medicaments against Enterococcus faecalis by diffusion method. 10 microliter root canal medicaments dropped on paperdisk and placed on nutrient agar media with enterococcus faecalis, then inhibition zone was calculated. Determination ability to kill enterococcus faecalis is done by inserting 1 ml medicaments root canal into 5 ml BHIB media, then 0.05 ml inoculum of Enterococcus faecalis inserted into each tube, except the negative control. 0.1 ml of each tube implanted in the media nutrient agar. Media incubated for 24 hours, then Enterococcus faecalis bacterial colonies that grow in media calculated using the CFU. Results. There no colony growth of enterococcus faecalis in both root canal medicaments. There are significant differences in inhibition zone of 2% chlorhexidine and 1% povidone iodine (p<0.05). Conclusion. Both of root canal medicaments can kill enterococcus faecalis, but chlorhexidine 2% was more capable inhibit Enterococcus faecalis.

Konsentrasi Bunuh Minimal (KBM) Ekstrak Propolis Lawang terhadap Candida albicans Alvita Wibowo; Ira Widjiastuti; Widya Saraswati
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Candida albicans is the most resistant fungi in root canals. The existence of Candida albicans after root canal treatment can cause the failure of root canal treatment. The prevalence of this case is 36,7%. Propolis is a resinous substance which has many pharmaceutical and biological effects such as antimicrobial activities. Its active components include flavonoid, saponin, and tannin, have an important role in antimicrobial activities. In this study, antifungal aspect of propolis extract was investigated. Purpose: The aim of this study was to determine the minimum fungicidal concentration (MFC) of propolis extract againts Candida albicans. Methods: This research was a laboratory experimental study. Propolis extract produced by propolis farm from Lawang. The antifungal activity test was performed by colony counting fungi in sabouraud’s dextrose agar (SDA) to determine the value of MFC of propolis extract againts Candida albicans. Result: At the concentration of 72,5%, 73,5%, 74,5%, 75,5%, 76,5%, and 77,5% there are decreases in the number of Candida albicans fungal colonies when compared with positive control group. There are significant differences in each study group (p<0,05). At the concentraion of 76,5% was not revealed any fungal growth of Candida albicans, it was because the antifungal effect of propolis extract has reached MFC. Conclusion: The minimum fungicidal concentration of propolis extract againts Candida albicans is 76,5%.

Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm Dalhar Hakiki; Latief Mooduto; Ketut Suardita; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcinia mangostana pericarp against E. faecalis bacterial biofilm. Methods:Laboratory experimental in-vitro with post test only group design. The method used is microtitter plate biofilm assay and continued with the readings use Elisa reader at a wavelength of 595 nm. Results:Flavonoids mangosteen pericarp effective as antibiofilm E.faecalis bacteria at a concentration of 12.5%. Conclusion:The study showed that flavonoids from mangosteen pericarp has antibiofilm activity against E. faecalis bacterial biofilm.

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DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

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Jawa timur

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Home Page

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Contact Name Dian Agustin W

Contact Email cdj@journal.unair.ac.id

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Journal Mail Official cdj@journal.unair.ac.id

Editorial Address DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location Kota surabaya, Jawa timur INDONESIA

Abstract

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Abstract

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Abstract

Contact Name
Dian Agustin W

Contact Email
cdj@journal.unair.ac.id

Phone
+62315030255

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cdj@journal.unair.ac.id

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DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location
Kota surabaya,

Jawa timur

INDONESIA