Claim Missing Document
Check
Articles

Found 23 Documents
Search

THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Carabelly, Amy Nindia; Taat Putra, Suhartono; Suardita, Ketut
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
PENGATURAN HAK PENGELOLAAN ATAS TANAH NEGARA PASCA BERLAKUNYA UU CIPTA KERJA Wijaya, Kadek Dwitya Partha; Suardita, Ketut
Kertha Desa Vol 9 No 7 (2021)
Publisher : Kertha Desa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan penulisan pada artikel ini yaitu untuk mengetahui dan memahami tentang ketentuan pengaturan hukum tentang Hak Pengelolaan atas tanah negara berdasarkan Undang-Undang Pokok Agraria Tahun 1960 dan untuk mengetahui dan menganalisa pengaturan hukum tentang Hak Pengelolaan atas tanah negara pasca berlakunya Undang-Undang Cipta Kerja. Penelitian ini menggunakan jenis penelitian yuridis-normatif didukung jenis pendekatan analisa konsep hukum dan perundang-undangan serta menggunakan teknik analisa data deskriptif kualitatif. Hasil kajian ini menunjukkan bahwa ketentuan perihal HPL atas objek tanah negara dalam UUPA belum terumuskan dalam norma yang ketentuannya rigid mengenai HPL di UUPA tidak tegas diatur kedudukan hukumnya. Namun, jika merujuk pada Penjelasan Umum II angka 2 UUPA bahwa negara dapat memberikan tanah yang dikuasai negara dengan hak untuk pengelolaan kepada badan hukum publik saja. Pasca berlakunya Undang-Undang Cipta Kerja, hak pengelolaan atas tanah negara akhirnya telah diatur dengan tegas di dalam Pasal 136 UU Cipta Kerja jo. Pasal 1 angka 3 PP No. 18 Tahun 2021. Pada intinya HPL merupakan hak menguasai dari negara yang kewenangan pelaksanaannya sebagian dilimpahkan kepada pemegang haknya untuk menggunakan dan memanfaatkan seluruh atau sebagian tanah hak pengelolaan untuk digunakan sendiri atau dikerjasamakan dengan pihak ketiga dengan perjanjian pemanfaatan tanah. Kata Kunci: Hak Pengelolaan, Tanah Negara, UU Cipta Kerja ABSTRACT This article aims to find out and understand the legal provisions regarding Management Rights over state land based on Undang-Undang Pokok Agraria Tahun 1960 and to find out and analyze legal arrangements regarding Management Rights over state land after the enactment of the Undang-Undang Cipta Kerja. This study uses a type of juridical-normative research supported by the type of approach to analyzing the concept of law and legislation and using qualitative descriptive data analysis techniques. The results of this study indicate that the provisions regarding Management Rights on state land objects in the Undang-Undang Pokok Agraria Tahun 1960 have not yet been formulated in norms whose rigid provisions regarding Management Rights in the Undang-Undang Pokok Agraria Tahun 1960 are not explicitly regulated by law. However, if referring to Penjelasan Umum II angka 2 Undang-Undang Pokok Agraria Tahun 1960 that the state can give land controlled by the state with rights for management to public legal entities only. After the enactment of the Undang-Undang Cipta Kerja, the right to manage state land has finally been firmly regulated in Article 136 of Undang-Undang Cipta Kerja jo. Article 1 point 3 PP No. 18 Tahun 2021. In essence, Management Rights is a right of control from the state whose implementation authority is partially delegated to the holder of the right to use and utilize all or part of the land with management rights for their own use or in cooperation with a third party with a land utilization agreement. Keywords: Land Management Right, State Land, UU Cipta Kerja
THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Amy Nindia Carabelly; Suhartono Taat Putra; Ketut Suardita
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2595

Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
The expressions of NF-kb and TGFb-1 on odontoblast-like cells of human dental pulp injected with propolis extracts Ira Widjiastuti; Ketut Suardita; Widya Saraswati
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (634.563 KB) | DOI: 10.20473/j.djmkg.v47.i1.p13-18

Abstract

Background: Propolis is known to have beneficial effects, namely anti- bacterial, anti-viral, anti-inflammatory, antioxidant, and immunomodulatory. Propolis extracts with anti-inflammatory properties are expected to be useful in treating inflamed pulp tissue with a diagnosis of reversible pulpitis. The inflammation of pulp tissue is caused by bacteria, namely Lactobacillus acidophilus. This research used odontoblast like cells derived from pulp tissue of human third molars. Odontoblast like cells exposed to Lactobacillus achidophilus were used as a model of proinflammatory cytokine signaling. This research examined the effects of propolis extracts on odontoblast like cells exposed to Lactobacillus acidophilus. Purpose: This research was aimed to determine the effectiveness of propolis extracts on the activities of odontoblast-like cells exposed to Lactobacillus acidophillus by measuring the expressions of NFkb and TGF- b1. Methods: First, pulp odontoblast cultures were derived from human dental pulp tissues of impacted third molars removed by using digestion method. Next, odontoblast-like cells exposed to inactive Lactobacillus acidophilus bacteria were given propolis extract. Finally, the activities of odontoblast-like cells were monitored by measuring the expressions of NF-kb and TGFb-1 with immunocytochemistry technique. Results: A decline NF-kb expression and on increase of TGFb-1 expression on odontoblast like cells exposed to inactive Lactobacillus acidophilus. Conclusion: Propolis extracts inhibit the expression of NF-kb, and increase the expression of TGF-b1 in pulp odontoblast-like cells exposed to inactive Lactobacillus acidophillus.Latar belakang: Propolis dilaporkan mempunyai efek menguntungkan yaitu bersifat anti bakteri, anti virus, anti inflamasi, anti oksidan, dan imunomodulator. Ekstrak propolis dengan sifat anti inflamasi diharapkan bermanfaat untuk mengobati jaringan pulpa yang mengalami inflamasi dengan diagnosis pulpitis reversibel. Inflamasi jaringan pulpa disebabkan oleh bakteri diantaranya adalah Lactobacillus acidophilus. Pada penelitian ini digunakan Odontoblast like cells yang berasal dari jaringan pulpa dari gigi molar ke tiga manusia. Odontoblast like cells dipapar Lactobacillus acidophilus digunakan sebagai model signaling sitokin proinflamasi. Studi ini, meneliti pengaruh pemberian ekstrak propolis pada odontoblast like cells yang dipajan Lactobacillus acidophilus. Tujuan: Penelitian untuk mengetahui efektifitas ekstrak propolis terhadap aktifitas odontoblast like cells yang dipajan Lactobacillus acidophillus dengan mengukur ekspresi NF-kb dan TGF-b1. Metode: pembuatan kultur odontoblas pulpa berasal dari jaringan pulpa gigi Molar ke tiga impaksi yang dicabut menggunakan metode digesti. Odontoblast like cells dipajan bakteri Lactobacillus acidophilus inaktif, diberi ekstrak propolis dan aktifitas dari odontoblast like cells diukur melalui ekspresi NF-kb dan TGFb-1 secara imunositokimia. Hasil: Terjadi penurunan ekspresi NF-kb, dan peningkatan ekspresi TGFb-1 pada kultur odontoblas yang dipapar bakteri Lactobacillus acidophilus inaktif. Simpulan: Ekstrak propolis menghambat ekspresi NF-kb, dan meningkatkan ekspresi TGF-b1 pada odontoblast like cells pulpa yang dipajan bakteri Lactobacillus acidophillus inaktif
The effective concentration of red betel leaf (Piper crocatum) infusion as root canal irrigant solution Fani Pangabdian; Slamet Soetanto; Ketut Suardita
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.984 KB) | DOI: 10.20473/j.djmkg.v45.i1.p12-16

Abstract

Background: Smear layer is a debris consisting of organic and inorganic particles of calcified tissue, necrotic tissue, pulp tissue, and dentinoblast and microorganism processes that can close the entrance to the dentin tubuli. Smear layer, will not only inhibit the penetration of disinfection materials and sealers to the dentin tubuli, but will also reduce the attachment of root canal filling material so that root canal irrigation solution is needed to dissolve the smear layer. Red betel leaf (Piper crocatum) infusion, on the other hand, contains saponin characterized as “surfactants” which can dissolve smear layer. Nevertheless, the effective concentration of the red betel leaf infusion has still not been known clearly. Purpose: This study is aimed to determine the effective concentration of the red betel leaf infusion for cleaning root canal walls from smear layer. Methods: Fiveteen extracted human teeth with straight single roots were randomized into 5 groups (n=3). The specimens were then shaped by using rotary instruments up to a size of 25/.07. During instrumentation, each canal was irrigated with 10, 20, 30 and, 40% red betel leaf infusion for treatment groups, while another was irrigated with aquadest for the control group. Root canal cleanliness was observed by using scanning electron microscope (SEM). Results: There were significant differences among treatment groups (p<0.05), except in the treatment groups irrigated with red betel leaf infusion with concentrations of 30% and 40% (p>0.05). Conclusion: It can be concluded that red betel leaf infusion with a concentration of 30% is effective for cleaning the root canal walls from the smear layer.Latar belakang: Smear layer adalah suatu debris yang mengandung partikel organik dan anorganik dari jaringan terkalsifikasi, jaringan nekrotik, proses dentinoblas, jaringan pulpa dan mikroorganisme yang dapat menutup jalan masuk ke tubuli dentin. Smear layer akan menghalangi penetrasi dari bahan disinfeksi dan sealer terhadap tubuli dentin dan mengurangi perlekatan bahan pengisi saluran akar, sehingga dibutuhkan larutan irigasi yang dapat membuang smear layer tersebut. Infusa daun sirih merah (Piper crocatum) mengandung saponin yang dikarakteristikkan sebagai surfaktan yang dapat melarutkan smear layer, tetapi sampai sekarang belum ada penelitian tentang hal tersebut. Tujuan: Penelitian ini dilakukan untuk mengetahui konsentrasi efektif daya pembersih infusa daun sirih merah (Piper crocatum) dapat membersihkan dinding saluran akar dari smear layer. Metode: 15 gigi premolar bawah manusia yang mempunyai akar lurus dibagi menjadi 5 kelompok secara acak (n=3). Gigi dipreparasi menggunakan rotary instrumen sampai Protaper F2 (30/0.02). Selama instrumentasi, dilakukan irigasi dengan infusa daun sirih merah (Piper crocatum) dan konsentrasi 10, 20, 30, 40% dan diirigasi aquadest untuk grup kontrol. Setelah itu kebersihan dinding saluran akar diperiksa dengan menggunakan scanning electron microscope (SEM). Hasil: Terdapat perbedaan yang signifikanantara masing-masing kelompok (p<0,05), kecuali kelompok yang diirigasi infusa daun sirih merah (Piper crocatum) konsentrasi 30% dan 40% (p>0,05). Kesimpulan: Dapat disimpulkan infusa daun sirih merah (Piper crocatum) dengan konsentrasi 30% efektif untuk membersihkan dinding saluran akar dari smear layer.
The potential application of stem cell in dentistry Ketut Suardita
Dental Journal (Majalah Kedokteran Gigi) Vol. 39 No. 4 (2006): December 2006
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.397 KB) | DOI: 10.20473/j.djmkg.v39.i4.p177-180

Abstract

Stem cells are generally defined as cells that have the capacity to self-renewal and differentiate to specialize cell. There are two kinds of stem cell, embryonic stem cell and adult stem cells. Stem cell therapy has been used to treat diseases including Parkinson’s and Alzheimer’s diseases, spinal cord injury, stroke, burns, heart diseases, diabetes, osteoarthritis, and rheumatoid arthritis. Stem cells were found in dental pulp, periodontal ligament, and alveolar bone marrow. Because of their potential in medical therapy, stem cells were used to regenerate lost or damage teeth and periodontal structures. This article discusses the potential application of stem cells for dental field.
Visualization of bubbles generation of electrical-driven EndoActivator tips during solutions activation in a root canal model and a modified extracted tooth: A pilot study Harry Huiz Peeters; Elvira Theola Judith; Ketut Suardita; Latief Mooduto
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 2 (2022): June 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i2.p71-75

Abstract

Background: EndoActivator, a sonically-driven canal irrigation system (Dentsply Tulsa Dental Specialties, Tulsa, OK), has been developed for activating root canal irrigants, and has recently been released onto the market. Purpose: To obtain an initial understanding of bubbles generation of electrical EndoActivator tips during activation of the irrigant in a transparent root canal model and a modified extracted tooth. Methods: A modified extracted tooth and a straight glass model were filled with a solution containing 17% EDTA or 3% NaOCl. A medium activator tip 22-mm polymer noncutting #25, 0.04 file driven by an electrical sonic hand-piece at 190 Hz (highest level) induced pressure waves that produced macro- and micro-bubbles. The physical mechanisms involved were visualized using a Miro 320S high-speed imaging system (Phantom, Wayne, NJ, USA) with high temporal and spatial resolutions. The imaging system acquired images at 25,000 frames per second with 320×x240 pixels per image, and attached a 60-mm f/2.8 macro lens (Nikon, Tokyo, Japan). Results: The end of the tip did not generate bubbles formation. Disruption of surface tension at the air–solution system in the glass canal model by an electrical sonic driven EndoActivator tip generated bubbles in the solution. However, it did not occur at the system of solution–air interfaces in the glass canal and modified extracted tooth. Conclusion: The physical mechanism of the solution activated by an electrical sonic driven EndoActivator tip in generting bubbles formation is because the surface tension at the air–solution system disruption. No bubbles formation occurred in the solution in the restricted space either in the solution-air system or modified extracted tooth. Better understanding of the physical mechanisms that relate specifically to the activation behaviour of EndoActivator tips in solutions is key to improving the cleaning mechanism that applies during root canal treatment.
Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity) Ivon Dewi Setianingrum; Ketut Suardita; Ari Subiyanto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.54 KB) | DOI: 10.20473/cdj.v7i1.2017.6-11

Abstract

Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries,  and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cavity on the first group used  0,78% saponin from mangosteen pericarp extract as cavity cleanser, the second group used 6% citric acid as cavity cleanser, and the control group used aquadest. Then, the teeth were split to be observed on Scanning Electron Microscope (SEM). Result: For Mann- Whitney test there were significant differences just between 078% saponin from mangosteen pericarp extract with 6% citric acid, and 6% citric acid with aquadest, but not for 0,78% saponin from mangosteen pericarp extract with aquadest. Median value of 6% citric acid showed 2,000 which is the smallest value compared to the value of the other groups. Conclusion: The cleanliness of cavity with 6% citric acid is better than that with 0,78%  saponin from mangosteen pericarp extract. 
Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm Dalhar Hakiki; Latief Mooduto; Ketut Suardita; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.493 KB) | DOI: 10.20473/cdj.v7i1.2017.18-22

Abstract

Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcinia mangostana pericarp against E. faecalis bacterial biofilm. Methods:Laboratory experimental in-vitro with post test only group design. The method used is microtitter plate biofilm assay and continued with the readings use Elisa reader at a wavelength of 595 nm. Results:Flavonoids mangosteen pericarp effective as antibiofilm E.faecalis bacteria at a concentration of 12.5%. Conclusion:The study showed that flavonoids from mangosteen pericarp has antibiofilm activity against E. faecalis bacterial biofilm.
EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Yuliana Dwiwahyu Suryandari; Ketut Suardita; M. Mudjiono; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.196 KB) | DOI: 10.20473/cdj.v7i2.2017.59-65

Abstract

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.