cover
Contact Name
Dr. Nuri Nurlaila Setiawan
Contact Email
editor3bio@sith.itb.ac.id
Phone
+62222511575
Journal Mail Official
support3bio@sith.itb.ac.id
Editorial Address
Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung Labtek XI, Jl. Ganesha 10 Bandung 40132 West Java - Indonesia
Location
Kota bandung,
Jawa barat
INDONESIA
3BIO: Journal of Biological Science, Technology and Management
ISSN : -     EISSN : 26558777     DOI : http://dx.doi.org/10.5614%2F3bio.2020.2.1
3Bio: Journal of Biological Science, Technology and Management is an interdisciplinary peer-reviewed journal in a wide aspect related to the field of life sciences and other related fields of study. The journal aims to promote scientific discourse and disseminate research on various branches and applications of bio-science, biotechnology and bio-based management. This journal invites original empirical research, literature reviews, theoretical or methodological contributions, or short communications on, but not limited to, the following topics: - Ecology and Biosystematics - Microbiology - Genetics and Molecular Biology - Animal Development and Physiology - Plant Development and Physiology - Entomology - Biomedical science - Biochemistry - Agronomy - Forestry - Bioengineering - Bioethics - Management of Biological Resources The journal also invites contributions from other associated disciplines. This journal is an open-access journal. Readers may read, download, copy, distribute, print, search, or link to the full texts of these articles without any charge, provided that readers acknowledge the Creative Commons attached to the articles. All submitted papers are reviewed by at least two referees before being accepted for publication, following a double-blind review process. This journal is delivered in an academic English and rigorously edited to provide a quality standard of a scientific journal. This journal is managed by the School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia.
Articles 5 Documents
Search results for , issue "Vol. 6 No. 2 (2024)" : 5 Documents clear
Business Model Canvas (BMC) Approach for Ecotourism Development Based on Islamic Boarding School Community (Case: Cipeujeuh Valley, Darul Arqam Muhammadiyah Islamic Boarding School) Al-Sakina, Nashiha; Yustiana, Yooce; Furqan, Alhilal
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.1

Abstract

This study investigates the potential for ecotourism in Cipeujeuh Valley, which is situated near the Darul Arqam Muhammadiyah Islamic boarding school in Garut Regency. The research examines the factors that drive tourists to visit the area, the level of community engagement, and the development of a community-based ecotourism business model. The data was gathered using descriptive statistics, the Ecotourism Opportunity Spectrum (ECOS) framework, and rapid rural appraisal techniques. The results indicate that Cipeujeuh Valley has intermediate ecotourism spectrum, with push factors such as sports and adventure, recreation and relaxation, social status, and quality time, and pull factors including nature and the local community, and enjoying facilities. The community is generally receptive to ecotourism development, with 61.3% expressing interest and 38.7% not. The study suggests that educational institutions, communities, organizations, and families can be targeted through a business model canvas that offers unique environments, sustainable resource use, community empowerment, educational tourism, and economic development. Key resources include natural attractions, religious and local wisdom activities, infrastructure, and a skilled workforce, while key activities involve nature-based and community-based tourism experiences.  
Application of Compost Tea and Ascorbic Acid to Increase Productivity and Antioxidant Activity of The Common Bean (Phaseolus vulgaris L.) Nasution, Ahmad Raihan; Dwiartama, Angga; Putra, Ramadhani Eka
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.4

Abstract

The common bean is a functional food rich in nutrients and bioactive compounds that support good health. Improving common bean yield is essential for enhancing nutrition within communities. This study examined the effects of compost tea, ascorbic acid, and their combination on the productivity and antioxidant activity of upright common beans. The experiment involved six treatments, including a control (water spraying) (P1), ascorbic acid (P2), manure compost tea (P3), BSF larvae production residue (BSFR) compost tea (P4), manure compost tea and ascorbic acid (P5), and BSFR compost tea and ascorbic acid (P6). All treatments, except P6, showed significant differences in productivity. P2 recorded the highest yield at 8,13 tons/ha/cycle and increased productivity by 19,03%, followed by P5 (9,22%) and P3 (3,95%) compared to P1 (control). P4 had a slight reduction in productivity by 3,95% than P1. A combination of compost tea and ascorbic acid increased productivity by 5,07% for P5 and 4,63% for P6 compared to the treatment without combination with ascorbic acid. P2 had the lowest IC50 value, 17,46 µg/ml, indicating the highest antioxidant activity. Compost tea and ascorbic acid combined reduced the IC50 value by 23,12% for P5, but P6 increased it by 32,59%. P5 improved the antioxidant activity of common bean pods when combined with compost tea and ascorbic acid, while P6 demonstrated a better effect without the combination. P3 and P6 had higher IC50 values than the control but lacked the potential to improve the antioxidant activity of common bean pods.
Biomonitoring of Air Quality Using Lichen as Bioindicator in The Greater Bandung Area, West Java Saragih, Dora Erawati; Irwanto, Rina Ratnasih; Taufikurahman, Taufik
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.3

Abstract

Air quality assessment in an area can be determined by conducting biomonitoring using bioindicator organisms, including lichen. Lichen is a symbiotic organism that passively absorbs nutrients and chemical compounds from the air. This research was conducted in the Greater Bandung area, including Bandung City and West Bandung Regency, to observe the abundance of lichen species so that the purity of the atmosphere could be evaluated at each research location and determine the effect of environmental variables on lichen abundance.The survey area was divided into 40 units spread over two locations in Bandung, namely Djuanda street (JD), Kebon Kawung street (KK), and two locations in West Bandung Regency, namely Padalarang street (PD) and Curug Cimahi (CC). CC locations with low levels of pollution were used as comparison areas. Lichen samples from observation locations were identified, and the number of colonies, frequency of closure, and diversity (H’) were counted so that the Index of atmospheric purity (IAP) value at each observation location could be known. As many as 24 species from 14 lichen families with a total of 256 colonies were found in the four observation sites with the percentage of thallus crustose (62%), foliose (37%), and fruticose (1%). The highest lichen diversity was found in the CC area (2.62), followed by JD (1.99), PD (1.63), and the lowest in KK (0.90). The lowest IAP result was in KK (10.21), followed by PD (17.70) and JD (31.85). The location with the highest IAP was obtained at CC (46.65), indicating that the environmental conditions were still good, while other locations were polluted.
The Influence of Polyethylene Glycol Precipitation Methods on Yield and Purity of White Radish Peroxidase Faizah, Nur Al; Giri-Rachman, Ernawati Arifin; Niloperbowo, Wardono
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.5

Abstract

Proteins are widely used in various industries as highly valued biotechnology products. One example is horseradish peroxidase isolated from horseradish (Armoracia rusticana) that used as enzyme label in immunochemistry. However, the cultivation of horseradish is limited to subtropical countries, making the dependency on horseradish peroxidase unsustainable for tropical countries. Numerous studies have explored alternative peroxidases, and white radish peroxidase isolated from Raphanus sativus L. has emerged as a promising candidate. In this study, white radish peroxidase is isolated using the polyethylene glycol (PEG) precipitation method which is widely used as a simple and cost-effective method. This study aims to evaluate the effectiveness of the one-step and two-step PEG precipitation method. The one-step PEG precipitation method used in this study was done by mixing the white radish juice with PEG 6000 30% (w/v), while the two-step method was done by mixing it with PEG 400 20% (w/v) and PEG 6000 30% (w/v) consecutively. This study compares the yield and recovery levels of total protein and white radish peroxidase, as well as the enzymatic specific activity of white radish peroxidase isolated both by the one-step PEG precipitation and the two-step PEG precipitation. The results indicate that both extraction methods yield the same level of white radish peroxidase. However, they differ in terms of purity. The two-step extraction method results in white radish peroxidase with higher purity, as evidenced by its specific activity towards the chromogen ABTS in the presence of H2O2.
Enhancing Lipid Extraction from Chlorella vulgaris Microalgae for Biodiesel Production: Application of Natural Deep Eutectic Solvent (NaDES) in Cell Disruption Priharto, Neil; Nadia Yasmin Dicky
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.2

Abstract

Natural deep eutectic solvent (NaDES) pre-treatment offers a promising alternative strategy to enhance lipid extraction efficiency from microalgae by influencing the integrity of the cell wall, thereby improving solvent accessibility to the cytoplasm and facilitating the release of intracellular lipid content. In this study, Chlorella vulgaris biomass underwent pre-treatment with four different NaDES formulations, each based on choline chloride (ChCl) with varying molar ratios: ChCl : Glycerol (1:2), ChCl : Glycerol (1:1), ChCl : Oxalic Acid (1:2), dan ChCl : Oxalic Acid (1:1).We analyzed the impact of these NaDES pre-treatments on lipid yield and fatty acid profiles. The extracted lipids exhibited an acid value of 42.56 mg KOH/g fat and a free fatty acid content of 0.25%. Samples subjected to NaDES treatment showed significant increases in lipid extraction efficiency, with lipid yields ranging from 1.25 to 2.3 times higher than those of untreated biomass extracted using hexane (p < 0.05). The highest lipid yield was observed in samples treated with ChCl : Glycerol (1:1), achieving a total lipid yield of 19.44% (w/w), more than double that of the untreated biomass. Although minor variations in fatty acid profiles were noted due to the NaDES treatment, the dominant fatty acids in each variation remained palmitic acid (C16:0, 21-29.5%) and oleic acid (C18:1, 13-43.88%).

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