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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

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Articles 10 Documents

Search results for , issue "Vol. 37 No. 2 (2014): Media Peternakan" : 10 Documents clear

In Vitro Testing to Aflatoxin Binding by Glucomannan Yeast Product and Glucomannan Extract from Amorphophallus oncophyllus A Susanto; EB Laconi; DA Astuti; S Bahri
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of research was to test the capability of glucomannan yeast product (GYP) and glucomannan resulted from Amorphophallus oncophyllus extraction (GRE) to bind aflatoxin in in vitro testing. Before in vitro testing, both GYP and GRE were analyzed to determine proximate analysis, glucose, and mannose concentrations. In vitro testing used aflatoxin, binder and gastro intestinal fluid in 3% ringer solution. The weights of binders were 41.05; 82.1; 123.15; and 164.2 mg and weight of aflatoxin was 0.1642 µg of each tube. The results showed that the percentage of aflatoxin bound increased by the increasing weight either glucomannan from yeast product or glucomannan resulted from A. oncophylus extraction. The percentages of aflatoxin binding with binder of both glucomannan yeast product were 19.72%; 21.51%; 42.25%; 46.35% and glucomannan from A. oncophyllus extraction were 4.08%; 28.72%; 36.73%; and 89.07%, consecutively. There were positive correlations (P

Bile Salt and Acid Tolerant of Lactic Acid Bacteria Isolated from Proventriculus of Broiler Chicken E Damayanti; H Julendra; A Sofyan; SN Hayati
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this research was to obtain the lactic acid bacteria (LAB) as probiotic candidates which have resistance to bile salt and acid condition. LAB was obtained using isolation method from proventriculus of broiler chicken. Selective MRS media with 0.2% CaCO3 addition were used for LAB isolation using pour plate sampling method under anaerobic condition. The result showed that four selected isolates had morphological and biochemical characteristics as LAB. The selected LAB was characterized as follow: antibacterial activities, antibiotic sensitivity, resistance on bile salt, gastric juice and acid condition, and biochemical identification. Antibacterial activities assay of cell free supernatant was confirmed using disc paper diffusion method which was arranged on factorial design and each treatment consisted of three replications. The cell free supernatant of LAB isolates had antibacterial activities against Escherichia coli, Pseudomonas aerugenosa, and Salmonella pullorum. Molecular identification procedure using 16S rRNA sequence analysis showed that R01 and R02 as Pediococcus acidilactici. The viability of the two isolates were tested by acid pH (pH 1, 2, and 3), gastric juice pH 2, and bile salt condition for digestives tract simulation. The result showed that R01 and R02 had a high viability percentages at pH 1, 2, and 3 (95.45%, 99.49%, 104.01%, and 67.17%, 120.74%, 103.4%, respectively) and at bile salt simulation for 1-2 hours (100.35%-102.71% and 100.02%-102.65%, respectively), but at gastric juice simulation for 1-2 hours, the P. acidilactici R01 had higher viability than P. acidilactici R02 (59.69%-76.53% versus 43.57%-40.69%, respectively). In the antibiotic sensitivity test for three antibiotics (i.e. erythromicin 15 µg, penicillin G 10 µg, and streptomycin 10 µg), the P. acidilactici R02 showed resistance to Streptomycin and Penicillin. It is concluded that P. acidilactici R01 and P. acidilactici R02 isolated from proventriculus of broiler chicken potential as probiotic candidates for chicken.

Fermentation Quality and in Vitro Nutrient Digestibility of Fresh Rice Straw-Based Silage Treated with Lactic Acid Bacteria B Santoso; B Tj Hariadi; V Sabariah; T Sraun
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of the experiment was to evaluate fermentation characteristics and in vitro nutrient digestibility of fresh rice straw-based silage ensiled with addition of epiphytic lactic acid bacteria (LAB) inoculant. The experiment was arranged in a completely randomized design, with 2 × 2 factorial arrangement of treatments. The first factor was the ratio of fresh rice straw (FRS), tofu waste (TW) and cassava waste (CW) consisted of two levels i.e., 40 : 20 : 40 and 40 : 25 : 35, on dry matter (DM) basis). The second factor was the level of LAB inoculant with two levels ie., 0 and 20 mL/kg FM. The treatments were (A) FRS + TW + CW with the ratio of 40 : 20 : 40, without LAB inoculant; (B) FRS + TW + CW with the ratio of 40 : 20 : 40 + LAB inoculant; (C) FRS + TW + CW with the ratio of 40 : 25 : 35, without LAB inoculant; (D) FRS + TW + CW with ratio of 40 : 25 : 35 + LAB inoculant. Results showed that addition of LAB inoculant in silage increased lactic acid concentration (P0.05) on chemical composition, fermentation quality of silage and in vitro digestibility. It was concluded that mixture silage with ratio of 40 : 20 : 40 with the addition of LAB inoculant had the best fermentation quality and nutrient digestibility than other silages.

Technical Efficiency of Beef Cattle Breeding Business in East Java Province L S Kalangi; Y Syaukat; S U Kuntjoro; A Priyanti
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objectives of this research were to compare technical efficiency of beef cattle business operating in lowland and upland areas of East Java and to identify and analyze factors affecting the technical efficiency as well as inefficiency of that business. A survey was conducted to 89 farmers in the lowland area of Probolinggo District and 97 farmers in the upland areas of Malang Districts during the period of February-March 2013. Results of stochastic frontier production function with MLE method showed that the average technical efficiency in lowland area was higher (80%) compared to that in upland area (64%). The results indicated that beef cattle production was highly significant and positively influenced by total of grasses and forages, rice straw, feed supplements, stock of cattle, labor time allocation and service per conception. However, location of the business, whether in lowland and upland areas was a significant factor that could increase the efficiency. Some factors that could significantly reduce the technical inefficiency of beef cattle business were: labor force in family, education level, proportion of beef cattle income to total household income, age of cattle sold, cattle health examination, ownership status of the cattle, and gender. Therefore, (1) the existing farmers group should be improved their role to facilitate trading of cattle feed, and (2) the government should facilitate the farmers in the provision of funding tu buy beef cattle with a subsidized interest rate, so the farmers will be more enthusiast in taking care of their cattle, thus could improve their production efficiency.

Body Resistance and Productive Performances of Crossbred Local Chicken Fed Inulin of Dahlia Tubers N Fajrih; N Suthama; V D Yunianto
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The research was aimed to examine the role of inulin as a prebiotic derived from dahlia flower tuber in the form of powder and extract as a prebiotic on body immunity of crossbred local chicken. The research was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). The treatments applied were T0: basal ration, T1: ration + 0.4% powder, T2: ration + 0.8% powder, T3: ration + 1.2% powder, T4: ration + 0.39% extract, T5: ration + 0. 78% extract, T6: ration + 1.17% extract. The birds were reared for conditioning from day 1 until day 21, and dietary treatment was given thereafter until 11 wk of age. The data were statistically analyzed according to ANOVA and continued to Duncan test at the level of 5% probability. The results showed that feeding inulin in the form of powder or extract significantly (P

Methane Mitigation and Microbial Diversity of Silage Diets Containing Calliandra calothyrsus in a Rumen in Vitro Fermentation System R Ridwan; I Rusmana; Y Widyastuti; K G Wiryawan; B Prasetya; M Sakamoto; M Ohkuma
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to investigate the effects of silage based diets on methane (CH4) mitigation and microbial diversity in a rumen in vitro fermentation. The experiment was arranged in a completely randomized design with five treatments and three replications. The dietary treatments consisted of varying levels of silage containing 50% Calliandra calothyrsus as follows K; 100% concentrate + pure tannic acid of 1 mg/mL, R1; 25% silage + 75% concentrate, R2; 50% silage + 50% concentrate, R3; 75% silage + 25% concentrate, and R4; 100% silage. The fermentation variables measured were total gas, CH4, in vitro organic matter digestibility (IVOMD), VFAs, pH, N-NH3, number of protozoa, and microbial diversity analysis. Increasing level of silages reduced total gas production, CH4 concentration, IVOMD, index of bacterial diversity, protozoal number, total methanogens and Methanobacteriales population. Diet with 25% to 50% silage decreased CH4 concentration, total gas production and IVOMD by 11.43%, 24.92%, and 18.73%, respectively. Ammonia N and VFAs (except butyrate and valerate) were significantly reduced (P

In Vitro Rumen Fermentation of Ration Supplemented with Protected Vegetable Oils N Hidayah; K G Wiryawan; S Suharti
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This experiment was designed to evaluate the effects of protected vegetable oils supplementation on in vitro fermentation characteristics, rumen microbial population, and methane production in cattle. The treatments were arranged in a complete randomized block design involving 2 factors i.e. oil type (sesame, canola, and flaxseed) and protection methods (non protected, calcium soap, and microencapsulation). Variables observed were rumen pH, N-NH3, total and molar proportion of VFA, dry matter and organic matter digestibility, population of protozoa and total bacteria, methane production, and hydrogen balance. Data were tested using Analysis of Variance (ANOVA) and the differences among treatments means were examined by Duncan Multiple Range Test. The oil type did not affect all variables measured. The protection method using microencapsulation significantly increased N-NH3 concentration. There was an interaction between oil type and protection method on total VFA concentration, molar proportion of VFA, and methane production. The supplementation of calcium soap-flaxseed oil significantly increased total VFA production, while the supplementation of microencapsulated flaxseed oil had the highest propionate concentration and H2 utilization, the lowest A:P ratio, and methane production. It is concluded that microencapsulated flaxseed oil was the best treatment to optimize rumen fermentation.

Single Nucleotide Polymorphisms (SNPs) in Exon 6 of Lecithin Cholesterol Acyltransferase (LCAT) Gene in Indonesian Local Sheep . Hidayati; Cece Sumantri; RR Noor; R Priyanto
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Lecithin cholesterol acyltransferase (LCAT) is a soluble enzyme that converts cholesterol and lecithin to cholesteryl esters and lysolecithins on the surface of high density lipoprotein and plays an important role in lipoprotein metabolism. The research was aimed to explore single nucleotide polymorphisms of LCAT gene in Indonesian local sheep. A total of 118 genomic DNA of Indonesian local sheep were used in this research, consisted of Sumatera Thin Tail (43 heads), Garut (19 heads), Javanese Thin Tail (17 heads), Javanese Fat Tail (6 heads), Rote Island (7 heads), Kissar (7 heads), Sumbawa (10 heads), and Lembah Palu (9 heads). Polymerase chain reaction was used to amplify genomic DNA for exon 6 (250 bp) and direct sequencing method was used to identify polymorphism sequences. The sequences were analyzed with BioEdit and MEGA 5.2 software. The BLAST sequence was obtained from Gene Bank GQ 150556.1. The results showed three novel SNPs, i.e. c.742C>T, c.770 T>A and c.882C>T. Substitution of cytosine to thymine c.742 is a synonymous mutation; thymine to adenine c.770 and cytosine to thymine c.882 are non-synonymous mutations. Polymorphisms of LCAT gene exon 6 was found in Sumatera Thin Tail, Javanese Thin Tail, Javanese Fat Tail, Garut, Lembah Palu, and Rote Island.

Analysis of Air Temperature Distribution in a Closed House for Broiler in Wet Tropical Climate A Yani; H Suhardiyanto; Erizal Erizal; BP Purwanto
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this research was to analyze the air temperature distribution in a closed house system for broiler in wet tropical climate by using computational fluid dynamics (CFD) to figure out the comfort level of the broiler in that system. The dimension of the experimental house was 100 m x 10 m x 2.4 m which could be occupied by 12,000 to 16,000 broiler chickens. The wall was made from expose mercy brick and curtain, plafond was made from galvanize, ventilation system used 6 fans with 1.27 m diameter and cooling pad and in-house lighting system used 60 units of 8 Watt lamps. The analysis of temperature distribution using CFD had high precision as was shown by the small number of average percentage of deviation (6.39%) between analyzed and measured results. Horizontally, the temperature distribution near the cooling pad was lower than others and continued to increase until near the position of the fan. In vertically, the air temperature in the area near the husks and the plafond were higher than the other mesurement points. At that height, the effective temperatures which were still comfort for the broiler were 20.07 oC, 19.81 oC and 20.04 oC at 12:20 h, 13:55 h and 15:20 h, respectively.

The Quality of Spermatozoa of Gembrong Goats during Cryopreservation Process F Pamungkas; A Batubara; Sutoro Sutoro
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Gembrong goat is an Indonesia local goat having specific characteristic that is currently categorized as a breed that is at risk of extinction. In this context, the cryopreservation of gametes is important to support a genome resource bank for storage of gametes for an indefinite period of time. Evaluation of semen and spermatozoa quality was performed to determine the survival of spermatozoa and this information will be used as a reference in the cryopreservation of semen and spermatozoa. The aim of this experiment was to study the characteristics of Gembrong goat’s semen and spermatozoa during cryopreservation process. Once a week, semen from three Gembrong goats (ages about 2-3 years old) was collected using artificial vagina and then frozen with TRIS extender. After freezing, the semen was thawed. Macro- and microscopic parameters of semen and spermatozoa were assessed in fresh and frozen-thawed semen. Results showed that in the fresh semen, the volume was 0.5 mL, sperm abnormalities was 5.74%, sperm concentration was 6731 x 106/mL, the sperm motility was 78.33%, live sperm was 83.17%, and sperm membrane integrity was 78.53%. After-thawing observation showed that sperm motility decreased to 49% (P

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