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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 522 Documents

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Potential of Lactic Acid Bacteria Isolated from Dangke and Indonesian Beef as Hypocholesterolaemic Agent H. Burhan; S. A. Priyambada; E. Taufik; I. I. Arief
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Lactobacillus fermentum strains were successfully isolated from dangke which was a fresh cheese-like product originating from Enrekang, South Sulawesi Province, Indonesia. In addition, Lactobacillus plantarum and Lactobacillus acidophillus were isolated from beef. This study aimed to investigate the ability of those 8 LAB strains from dangke and beef in lowering cholesterol level by using in vitro study. Strain of Lactic acid bacteria used were L. fermentum strains (A323L, B111K, B323K, C113L, C212L), L. plantarum strains (IIA-1A5 and IIA-2C12), and L. acidophillus IIA-2B4. Variables observed were identification of Bile Salt Hydrolase (BSH) gene by Polymerase Chain Reaction (PCR), BSH activity and cholesterol assimilation. Phylogenetic tree indicated homology of L. plantarum IIA-IA5 was 98% to BSH gene of L. plantarum Lp529 with access code of FJ439771 and FJ439775 obtained from GenBank. The results demonstrated that eight strains of LAB isolated from dangke and beef that potentially showed cholesterol-lowering effects were L. fermentum B111K and L. plantarum IIA-1A5. L. fermentum B111K was able to assimilate cholesterol by 4.10% with assimilated cholesterol of 0.13 mg in 1010 cells. In addition, L. plantarum IIA-1A5 had BSH gene and BSH activity, as well as the ability to assimilate cholesterol by 8.10% with assimilated cholesterol of 0.06 mg in 1010 cells. It is concluded that L. fermentum B111K and L. plantarum IIA-1A5 were strains that showed cholesterol-lowering effects.

The Relationship Analysis between Service Performances of Milk Producer Cooperative with the Dairy Farm Performance of Members A. Asmara; Y. L. Purnamadewi; D. Lubis
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The main objective of Milk Producers Cooperative (MPC) is to provide services to the members and improve the performance of dairy farm of MPC members. The purposes of this study were: (1) to analyze dairy farm performance of MPC members, (2) to analyze performance of MPC services, and (3) to analyze the relationship between service performance of MPC with dairy farm performance of MPC members. The main data used were primary data. The analytical methods used included: descriptive analysis, profit analysis and R/C Ratio, and Chi-Square. The study showed that productivity and profitability of large-scale dairy farm was higher than small-scale. The performance of small-scale dairy farm of MPC members was relatively low both in terms of productivity and profitability. Most members judged that the performance of MPC services was good. There was a positive relationship between the MPC service performance (milk marketing, finance, technical assistance, and education/training) with the performance of dairy farm of MPC members. Coaching and more intensive services should continue to be given by the MPC to the members, especially the members with the small-scale dairy farms.

Insulin-Like Growth Factor-I Concentration in the Follicular Fluid of Bali Cattle and Its Role in the Oocyte Nuclear Maturation and Fertilization Rate H. Hasbi; S. Gustina; N.W. K. Karja; I. Supriatna; M. A. Setiadi
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to determine the concentration of IGF-I in the follicular fluid (FF) of Bali cattle and its role in the nuclear maturation and fertilization rate. The follicular fluid was collected by the aspiration technique, then it was centrifuged at 1500 g for 30 min at 24oC. The supernatant was collected and stored at -20oC until being used in the experiment for analysis of IGF-1. A total of 1105 oocytes were used in this study. The oocytes were matured in M199 without supplementation of bovine serum albumin, with supplementation of BSA, and with supplementations of 10% FF (v/v) from the follicle with diameter Ø<4 mm, 4≤Ø<6 mm, 6≤Ø<8 mm, and Ø≥8 mm at the luteal phase and then fertilized. The results showed that the concentrations of IGF-I in the FF obtained during the luteal phase was significantly higher (P<0.05) compared to those obtained during follicular phase. The IGF-I concentrations in the follicular fluid of follicle with diameter smaller than 6 mm were significantly higher (P<0.05) compared to those with diameters larger than 6 mm. The percentage of nuclear maturation rate of oocytes cultured with FF obtained from follicle with diameter <4 mm was significantly higher (P<0.05) compared to those obtained from the other groups of follicle diameters. The supplementation of maturation media with BSA and FF were able to improve fertilization rate significantly (P<0.05) compared to maturation media without BSA. In conclusion, the concentration of IGF-I in the follicular fluid obtained during the luteal phase was higher compared to those obtained during the follicular phase. The IGF-I concentrations in the follicular fluid of smaller follicles (diameter <6 mm) were higher compared to those in the large follicles (diameter ≥6 mm). The supplementation of FF can improve the nuclear maturation and fertilization rate

Anacardic Acid Isolated From Cashew Nut Shell (Anacardium occidentale) Affects Methane and Other Products in the Rumen Fermentation A. Saenab; K. G. Wiryawan; Y. Retnani; E. Wina
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Biofat is a hexane extract containing several bioactive compounds with anacardic acid as the major compound. This study aimed to examine the effect of anacardic acid on rumen fermentation, especially methane and its degradation in the in vitro rumen fermentation. The study was arranged in a completely randomized block design. The treatments were control (substrate or complete feed), biofat (substrate + 0.75 uL/mL biofat), and anacardic acid (substrate + 0.75 uL/mL anacardic acid). Measured variables were total gas production, methane, pH, concentration of ammonia (NH3), dry matter degrability (DMD), organic matter degrability (OMD), and neutral detergent fiber degrability (NDFD) in the rumen. The chromatogram GC-MS analysis results indicated that the anacardic acid isolation process of the biofat produced nearly pure isolate (99.44%), and significantly decreased the production of methane by 51.21% and 39.62%, respectively. Anacardic acid degradation pattern in the in vitro rumen test showed a shifting of retention factor (Rf) value after anacardic acid being incubated with the degradation of anacardic acid occurred after 24 h of fermentation. In conclusion, anacardic acid isolated from biofat has a dominant role to reduce the in vitro methane production. Anacardic acid is very potential to be used as a methane reducing agent.

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken Soni Sopiyana; M. A. Setiadi; M. Fahrudin; I. Supriatna
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Primordial germ cells (PGCs) are cells that will differentiate themselves into spermatogonia in the testis or oogonia in the ovary. Primordial germ cells arise from epiblast and circulate through the bloodstream and finally entering gonadal anlage. The aim of this study was to determine the number of gonadal PGCs of KUB chicken at different development stages. Sixty KUB chicken fertile eggs were divided into four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 oC with a humidity of 60%. Harvesting was synchronized to the embryonic development at 6-9 d. Gonads were collected using sharp tweezers, and were placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were purified using PBS [-]. The results showed that the average number of gonadal PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo, respectively. Number of gonadal PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P<0.05), which reached a peak at day 7 of incubation, so that the isolation and collection of PGCs from the gonads were recommended at day 7 of incubation. This information is useful in production of germline chimera of other Indonesian local chickens.

Physicochemical Characteristics, in Vitro Fermentation Indicators, Gas Production Kinetics, and Degradability of Solid Herbal Waste as Alternative Feed Source for Ruminants A. N. Kisworo; A. Agus; Kustantinah Kustantinah; B. Suwignyo
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aims of this research were to study the nutrient and secondary metabolite contents of solid herbal wastes (SHW) that were preserved by freeze drying, sun drying and silage, as well as to analyze their effects on in vitro fermentation indicators i.e., gas production kinetics and degradability of solid herbal waste. Physical and chemical properties on three forms of SHW (sun dry, freeze dry, and silage) were characterized and then an in vitro gas production experiment was performed to determine the kinetics of gas production, methane production, NH3, microbial protein, and SHW degradability. Polyethylene glycol (PEG) was added to the three treatments to determine the biological activity of tannins. Results showed that all three preparations of SHW still contained high nutrient and plant secondary metabolite contents. Gas production, methane, NH3, microbial protein, in vitro degradability of dry matter (IVDMD) and organic matter (IVDOM) of SHW silage were lower (P<0.05) compared to sun dry and freeze dry. These results were apparently due to the high content of secondary metabolites especially tannin. It can be concluded that solid herbal wastes (SHW) can be used as an alternative feed ingredients for ruminants with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen.

Potency of Culled Saanen Crossbred Goat in Supplying Raw Meat for Traditional Thai Butchery A. A. Putra; S. Wattanachant; C. Wattanachant
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Potency of culled Saanen crossbred goat meat to replace the supply of yearling Boer crossbred goat meat was evaluated. Selected muscles from leg and shoulder cuts were analysed for their nutritional (proximate composition, collagen, amino acids, fatty acids), physicochemical (pH, myoglobin, drip loss, cook loss, shear force, lightness (L*) redness (a*) yellowness (b*), microstructure), and sensory (triangle test, hedonic test) evaluations. Meat samples from culled Saanen crossbred goat exhibited higher values in protein, collagen, and MUFA (P<0.05) than those from Boer crossbred goat, while lower values in ash, soluble collagen, and SFA were obtained (P<0.05). Meat from culled Saanen crossbred goat revealed higher cook loss, shear force, and redness compared to those from a yearling Boer crossbred goat (P<0.05). In addition, thicker perimysium in meat of Saanen crossbred goat was obtained particularly that could be seen on leg part. In sensory evaluation result, the panels could detect the differences between raw meat characteristics of these goat breeds (P<0.05) within the same muscle. However, the panels could not distinguish the difference between breeds in leg meat after being cooked. Shoulder meat of Saanen crossbred goat had less acceptance level compared to the other samples (P<0.05) particularly on its texture and taste quality. In summary, shoulder cut of culled Saanen crossbred goat exhibited a well-intentioned potency to substitute the supply of meat from yearling Boer crossbred goat. Nevertheless, pre-treatment might be applied to leg cut of Saanen crossbred goat to solve the less acceptance level of its textural and taste characteristics.

Characteristics of Sie Reuboh Supplemented with Different Combinations of Palm Vinegar (Arenga pinnata) and Kaffir Lime Leaves (Citrus hystrix) Masyitah Masyitah; I. I. Arief; T. Suryati
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed to analyze the characteristics of sie reuboh processed with the addition of palm vinegar and kaffir lime leaves with different concentrations to produce sie reuboh with a good quality and to maintain the stability of its quality during storage at room temperature. The research consisted of 2 stages. In the first stage, 4000 g of sie reuboh was treated with different doses of palm vinegar and kaffir lime leaves (0 mL and 0 g, 100 mL and 10 g, 120 mL and 20 g, and 140 mL and 30 g) for microbiological analysis. Sie reuboh with the best result of microbiological analysis was later used in Stage 2 to be stored at room temperature and was analyzed for its physical antioxidant activity, thiobarbituric acid reactive substance (TBARS), and microbiological analysis. The result showed that different levels of palm vinegar and kaffir lime leaves did not significantly affect the aw, pH, yield, and microbiological characteristics of the sie reuboh. Different ratios of palm vinegar and kaffir lime leaves significantly affected (P<0.05) the tenderness of sie reuboh. Sie reuboh without addition of palm vinegar and kaffir lime leaves showed a better tenderness than sie reuboh added with palm vinegar and kaffir lime leaves. The best treatment based on microbiological analysis was shown by treatment of sie reuboh with 120 mL of palm vinegar and 20 g of kaffir lime leaves and this sie reuboh was later used to test the stability of the sie reuboh when it was stored at room temperature. The results showed that sie reuboh produced by addition of 120 mL of palm vinegar and 20 g of kaffir lime leaves demonstrated antioxidant activity for inhibition of free radicals as was indicated by the stability of TBARS value during 9 days of storage at room temperature. This combination of treatment could also effectively stabilize the quality of sie reuboh, as indicated by a proper storability at maximum limit of total plate count and mold growth until 3 days of storage at room temperature.

Risk Based Milk Pricing Model at Dairy Farmers Level W. Septiani; Marimin Marimin; Y. Herdiyeni; L. Haditjaroko
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The milk price from a cooperative institution to farmer does not fully cover the production cost. Though, dairy farmers encounter various risks and uncertainties in conducting their business. The highest risk in milk supply lies in the activities at the farm. This study was designed to formulate a model for calculating milk price at farmer’s level based on risk. Risks that occur on farms include the risk of cow breeding, sanitation, health care, cattle feed management, milking and milk sales. This research used the location of the farm in West Java region. There were five main stages in the preparation of this model, (1) identification and analysis of influential factors, (2) development of a conceptual model, (3) structural analysis and the amount of production costs, (4) model calculation of production cost with risk factors, and (5) risk based milk pricing model. This research built a relationship between risks on smallholder dairy farms with the production costs to be incurred by the farmers. It was also obtained the formulation of risk adjustment factor calculation for the variable costs of production in dairy cattle farm. The difference in production costs with risk and the total production cost without risk was about 8% to 10%. It could be concluded that the basic price of milk proposed based on the research was around IDR 4,250-IDR 4,350/L for 3 to 4 cows ownership. Increasing farmer income was expected to be obtained by entering the value of this risk in the calculation of production costs.

Nutrient Changes and in Vitro Digestibility in Generative Stage of M10-BMR Sorghum Mutant Lines R. Sriagtula; P.D.M.H. Karti; L. Abdullah; Supriyanto Supriyanto; D. A. Astuti
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this research was to investigate the influences of generative stage on crude protein, crude fiber, ash, and crude fat contents as well as in-vitro dry matter and organic matter digestibilities of M-10 BMR sorghum mutant lines. This research was arranged into a randomized block design with 2 factors. The first factor was M-10 BMR sorghum mutant lines (Patir 3.1, Patir 3.2 and Patir 3.7) and the second factor was generative stages (flowering, soft dough and hard dough phase). The observed variables were proximate contents of stem, leaves and panicle of sorghum plant and in-vitro digestibility of whole plant. The results showed that leaves crude protein (CP) was more influenced by M-10 BMR sorghum mutant lines. Stems and panicles CP were influenced by the interaction between M-10 BMR sorghum mutant lines and generative stages. Further generative stage reduced stems CP but increased panicles CP. Crude fiber (CF), ash, and ether extract (EE) in leaves were not influenced by generative stages. Stems CF was influenced by M-10 BMR sorghum mutant lines and generative stages, while stems EE was more influenced by generative stages. Stems ash content was influenced by the interaction between M-10 BMR sorghum mutant lines and generative stages while panicles ash content was more influenced by generative stages. M-10 BMR sorghum mutant lines and hard dough phase increased in-vitro dry matter and organic matter digestibilities. Based on those findings, it can be concluded that the increased maturity reduces CP and CF contents so it increases in-vitro digestibilities.

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