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MEDIA PETERNAKAN - Journal of Animal Science and Technology
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Core Subject : Agriculture,
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Articles 522 Documents
In Vitro Rumen Fermentation Characteristics and Fatty Acid Profiles Added with Calcium Soap of Canola/Flaxseed Oil S. Suharti; A. R. Nasution; K. G. Wiryawan
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.079 KB) | DOI: 10.5398/medpet.2017.40.3.171

Abstract

This research aimed to assess the effect of adding canola oil and flaxseed oil which were protected with calcium soap (Ca-soap) on the fermentation characteristics, rumen microbial population, and the profile of fatty acids in the rumen during 4 and 8 hours in the in vitro fermentation. The research design used in this study was a completely randomized block design with 3 treatments and 4 replications. The treatments consisted of control ration (Napier grass and concentrate at the ratio of 60 : 40), control + 6% of Ca-soap of canola oil, and control + 6% of Ca-soap of flaxseed oil. Variables observed were pH value, NH3 concentration, volatile fatty acid (VFA), dry matter and organic matter digestibility, and fatty acid profile.  The results showed that the addition of Ca-soap of canola or flaxseed oil did not affect the pH value, NH3 concentration, dry matter digestibility, organic matter digestibility, total population of bacteria and protozoa in the rumen. However, the total production of ruminal VFA was increased (P<0.05) with the addition of Ca soap of canola oil/flaxseed oil. The use of Ca-soap of flaxseed oil increased (P<0.05) the content of unsaturated fatty acids in the rumen at 4 h incubation. The addition of Ca-soap of flaxseed oil resulted the lowest (P<0.05) level of unsaturated fatty acids biohydrogenation compared to the other treatments at 4 h incubation. In conclusion, the addition of Ca soap of canola/flaxseed oil could improve VFA total production. Vegetable oils protected using calcium soap could inhibit unsaturated fatty acid biohidrogenation by rumen microbes. Ca-soap of flaxseed oil could survive from rumen biohydrogenation in the rumen better than Ca-soap of canola oil.
Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure M. Y. Hidayat; H. M. Saud; A. A. Samsudin
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.376 KB) | DOI: 10.5398/medpet.2017.40.3.178

Abstract

In this study, the isolation of sulphur oxidising bacteria (SOB) from hot spring in Malaysia was carried out in an enrichment culture using sodium thiosulphate as a sole energy and CO2 as a sole carbon source. A total number of 80 SOB isolates were obtained from the agar plate and considered as positive SOB due to their abilities using thiosulphate for growth. All the isolates were initially screened for their fast growths in liquid medium and 13 isolates were selected for another screening process. Three SOB isolates namely isolate AH18, AH25, and AH28 were selected based on their abilities to grow faster, produce the highest sulphate ion and reducing the pH in the growth medium. The cells were Gram-negative and short rod-shaped. The effects of various variables including temperature (25-45 °C), pH (4-9), sodium thiosulphate concentrations (4-100 mM) and metabolic characteristic were evaluated on bacterial growth and their sulphur oxidation activities. The optimum pH of all the potential isolates occurred at pH 8.0. Meanwhile, the optimum temperature for isolate AH18, AH25 and AH28 occurred at 45 °C, 30 °C, and 30-45 °C, respectively. The three isolates were classified as facultative chemolithotroph with the capability of growth in thiosulphate concentration as high as 100 mM. Therefore, given the ability in the oxidation of thiosulphate, temperature and pH adaptabilities, with the metabolic flexibilities of isolates AH18, AH25, and AH28 could be a good H2S biological deodorizing candidate.

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