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Dr. Tri Wahyu Suprayogi, drh., M.Si
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Redaksi Jurnal Departemen Kesehatan, Fakultas Vokasi Gedung A, Universitas Airlangga Jl. Dharmawangsa Dalam No. 28-30, Surabaya 60286 Telepon : 031-5033869, 031-5053156 Ext. 129; Fax : 031-99005114 E-mail: javest@journal.unair.ac.id
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INDONESIA
Journal of Applied Veterinary Science and Technology
Published by Universitas Airlangga
ISSN : 27161188     EISSN : 2716117X     DOI : http://dx.doi.org/10.20473/javest
Core Subject : Health,
Veterinary
The scope of this journal is all about Veterinary Science and Technology field such as Animal Nutrition, Applied Veterinary Reproduction, Applied Veterinary Parasitology, Applied Veterinary Microbiology, Veterinary Public Health, Applied Veterinary Clinic. Journal of Applied Veterinary Science and Technology accepts submission from all over the world.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 95 Documents
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Characterization of Amino Acid Mutations of Newcastle Disease Virus (NDV) In Swan Geese (Anser cygnoides) In East Java, Indonesia Naimah Putri; Ine Karni
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.1-6

Abstract

Background: Newcastle disease (ND) is a highly contagious viral disease affecting the poultry industry. The NDV is classified into three strains based on their relative virulence, namely velogenic or highly virulent, mesogenic or moderately virulent, and lentogenic or lowly virulent. The clinical manifestations of the disease vary depending on many factors, such as host susceptibility and the virulence of the NDV strain. Objective: This study aims to analyze the amino acid mutations of the NDV in unvaccinated swan goose (Anser cygnoides) from various locations in Java. Methods: Samples were collected through cloacal swabs and isolated by inoculation in Specific Pathogen-Free (SPF) embryonated eggs that were nine days old. Hemagglutination and hemagglutination inhibition tests were conducted to confirm that the isolated virus was NDV. The isolated virus was processed using reverse transcription-polymerase chain reaction (RT-PCR) with primers that amplified partial sequences of the fusion (F) gene, which was analyzed to determine the pathotype. Results: The results indicated the presence of mutations in several regions. The amino acid changes occurred in 17 variable sites (7.2%) between RefSeq/JF950510 and ND/SW1/2018, 12 variable sites (5.1%) between RefSeq/JF950510 and ND/SW2/2018, 13 variable sites (5.5%) between RefSeq/JF950510 and ND/SW3/2018, and 19 variable sites (8.1%) between RefSeq/JF950510 and ND/SW4/2018. The amino acid sequences of the cleavage site of the fusion (F) protein revealed that all isolates had low virulence. Conclusion: The results indicated that mutations in the region outside the cleavage site not were incapable of altering the virulence of the virus.
Literature Study : Histopathology Analysis of Rabies Disease (2014 - 2022) Afifah Yudining Dwi Andadari; Dyah Ayu Oktavianie A. Pratama; Andreas Bandang Hardian; Handayu Untari
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.93-98

Abstract

Background: Rabies is a contagious and zoonotic disease caused by Lyssavirus, a neurotropic, negative-sense, single-stranded RNA virus. Rabies infects most mammals through the transmission of saliva from a rabid animal that enters the body of a healthy animal through an open wound, bite, scratch, or direct contact. A post-mortem diagnosis and evaluation are crucial for establishing regional statistical data in order to design a robust strategy for combating rabies. Histopathology has long been known to be an invaluable source of information regarding the destructive impact of rabies in nervous tissue. In addition to other ante-mortem evaluations, a correlation between clinical signs, gross pathological findings, and histopathological findings must be established to achieve the maximum target of combating rabies. Purpose: This study aims to analyze and compare gross pathological findings and histopathological findings in the literature published between 2014 and 2022. Methods: A comparison between gross pathological findings and histopathological findings was conducted on case studies from 2014 to 2022. Anamnesis and clinical signs were obtained as supporting data. The findings were presented as percentage to identify the predominant pathological signs. Results: A review of four case studies revealed that paralysis, hyperesthesia, gait abnormality, and muscle tremor were the most commonly observed signs (50%). Only two case studies revealed gross pathological findings, including meningeal congestion and cerebellar hemorrhage. Eight studies identified the intracytoplasmic negri body in Purkinje cells as the most commonly observed histopathological finding (100%). Conclusion: The primary clinical signs and gross pathological findings associated with rabies are typically regarded as non-specific. Histopathology revealed the intracytoplasmic Negri body in Purkinje cells as the most commonly observed finding (100%).
Effects of Dietary Soy Sauce Dregs and Cassava Peel Meal on the Performance of Production and Reproduction of Grower Quails (Cortunix cortunix Japonica) Muhammad Misbahul Ummah; Wahib Moh.Ridho Rojabi; Rosa Tri Hertamawati
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.7-11

Abstract

Background: The cost of feed is a significant factor in the viability of a quail farming business. Purpose: This study aims to determine the effects of dietary soy sauce dregs and cassava peel meal on the performance production and reproduction of grower quails (Cortunix cortunix Japonica). Methods: This study employed four treatment groups, each with five replicates, namely P0 (control), P1 (5%), P2 (10%), and P3 (15%). The parameters observed were feed consumption, body weight gain, feed conversion ratio, mortality, body weight at sexual maturity, egg weight, and laying age. Data analysis was conducted using the analysis of variance (ANOVA) test, followed by the Duncan's multiple range test (DMRT) to identify any significant differences. Results: The results showed that the addition of soy sauce dregs and cassava peel meal to the feed up to 15% to the treatment group resulted in significant effects (p < 0.05) on feed consumption, egg weight, and laying age. However, it had no significant effect on body weight, feed conversion ratio, mortality, and body weight at sexual maturity. Conclusion: The supplementation of soy sauce dregs and cassava peel meal up to 15% in grower quail diets resulted in improvements in the performance of both production and reproduction of grower quails, as evidenced by increased feed consumption, egg weight, and laying age.
A Retrospective Study on the Mortality Rate of Camel Calves, Leading Causes, and Associated Risk Factors in Borana Zone, Oromia Regional State, Ethiopia Gerade Abduljami; Adem Kumbe
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.12-19

Abstract

Background: Camelus dromedarius (one-humped camel) is an important livestock species uniquely adapted to arid and hot environments. Purpose: This study aims to determine the mortality rate of camel calves and to investigate the potential risk factors associated with it. Methods: Retrospective data were collected through interviews with livestock owners using a semi-structured questionnaire from February 2020 to January 2021. During the study period, a total of 1,249 calves were born. This allowed for assessment of annual mortality rate and its main contributing factors. Results: The average annual mortality rate was 26% (1.29 ±1.77), while the overall mortality rate was 55% (1.30 ± 1.85). Malnutrition (5%), unexplained causes (6%), and diseases (24%) were the three leading causes of calf mortality. Diarrhea (57%), nonspecific diseases (45.4%), respiratory diseases (23%) were significant among the diseases. Mistakes in the management of young livestock included limiting access to colostrum and milk for human consumption, as well as poor care and health management. Conclusion: The results of this study suggested that the pastoral livestock production system used in Borena has a serious problem with calf mortality. The first month of life had the highest mortality rate of 26%. Diseases, starvation, and unexplained causes were the leading causes of calf mortality. In addition, calf mortality was associated with the timing of birth and colostrum feeding.
Effect of Artemisia vulgaris Supplementation on Zebrafish Embryo Under Heat Stress Condition during In Vitro Culture Habib Syaiful Arif Tuska; Nursalsabila Khamalt; Muhammad Arfan Lesmana; Reza Yesica; Viski Fitri Hendrawan; Budiono; Gretania Residiwati
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.20-25

Abstract

Background: Artemisia vulgaris contains flavonoids, which play a vital role in counteracting free radicals. Purpose: To determine the effect of Artemisia vulgaris extract supplementation on embryo development, heart rate and survival of zebrafish under heat stressed and non-heat stressed conditions. Methods: The research used a completely randomized design. Zebrafish embryos (n=240) were divided into heat stressed (36°C) and non-heat stressed (28°C) groups, while for each group were divided into three subgroups, namely T1/control (without Artemisia vulgaris supplementation); and supplemented group T1 and T2, with 2 µL and 4 µL of Artemisia vulgaris supplementation, respectively. The efficacy of Artemisia vulgaris supplementation was determined by observing the embryo development, heart rate, and survival rate of zebrafish up to 96 hours post fertilization (hpf). Results: The development of zebrafish embryos under heat stressed treated with Artemisia vulgaris extract gave the same quality as the control treatment without heat stressed exposure. Zebrafish embryos exposed to heat stressed with 4 µL Artemisia vulgaris supplementation gave the highest survival rate on the heat stressed group. Artemisia vulgaris supplementation improved the heart rate of zebrafish exposed to heat stressed as in the non-heat stressed group. Conclusion: Artemisia vulgaris extract can reduce the detrimental effects of heat stressed induction on zebrafish embryos, as evidenced by the improvement in embryonic development, heart rate, and survival rate of zebrafish embryos after supplementation.
Detection of Fasciola sp. in the Livers of Slaughtered Cattle at a Slaughterhouse in North Lombok Regency Syamsuddin Syamsuddin; Novarina Sulsia Ista'in Ningtyas; Ni Luh Lasmi Purwanti; Supriadi Supriadi
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.26-30

Abstract

Background: Fasciola sp. has been identified as a highly pathogenic organism and has a significant health impact on both animals and humans. However, there is a lack of data and information on fasciolosis in North Lombok Regency. Purpose: This study aims to detect the presence of Fasciola sp. in livers of slaughtered cattle at the Tanjung Sub-district Slaughterhouse, North Lombok Regency. Methods: This study was conducted on the livers of slaughtered cattle, with a total of 30 samples collected between August and September 2023. Results: A total of 30 liver samples from slaughtered cattle at the Tanjung Sub-district Slaughterhouse, North Lombok Regency were examined for the presence of Fasciola sp. worms. The examination involved observing changes in color, size, consistency, and palpation of the organ. Following the incision of the liver and bile ducts and subsequent inspection of several areas with pathological-anatomical changes due to the infestation of Fasciola sp., an average length and width measurements of Fasciola sp. worms were found to be 26 mm and 6.4 mm, respectively. This indicated the worms belong to the Fasciola gigantica species. Conclusion: The results of this study indicated that Fasciola sp. worms were found in the livers of slaughtered cattle at the Tanjung Sub-district Slaughterhouse, North Lombok Regency. The worms were identified as belonging to the Fasciola gigantica species, characterized by an average length of 26 mm and width of 6.4 mm, having a narrow shoulder, a blunt posterior end, a flat leaf-like body shape, dorsoventrally flattened, a brownish-gray color, and an oral sucker at the anterior end.
Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL Putu Angga Wiradana; Anak Agung Ayu Putri Permatasari; Ni Kadek Yunita Sari; I Gede Widhiantara; I Made Gde Sudyadnyana Sandhika; Ernawati Waruwu; Jatu Maranatha Fimaputra; Novaria Sari Dewi Panjaitan; Fadillaisyia Riandani Putri; Arif Nur Muhammad Ansori

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Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: The marine environment is the main source of research on natural products in the future. In addition, marine microorganisms have been identified as a natural source capable of developing new antibiotic compounds, including controlling urinary tract infections caused by Escherichia coli ESBL. Purpose: This study aims to isolate, select, and test the potential of brown macroalgae endophytic fungi (Phaeophyceae) collected from the coastal waters of Sanur, Bali Province. Methods: Three types of brown algae were collected from Sanur Beach and their endophytic fungi were isolated using PDA media. Antibacterial activ
Anthrax: Distribution, Knowledge, Attitude and Practice Towards Pastoralists in Jawhar, Qalimow and Adan Yabal Somalia. Abdirahman Barre; Abdihamid Salad Hassan; Abdullahi Nur Mohamed
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.42-47

Abstract

Anthrax is a Zoonotic Disease (both Human and Animals) the disease is causing higher morbidity and mortality rate in Middle-Shebelle Somalia. Anthrax is a rare infectious disease caused by the bacterium Bacillus anthraces. It was reported in many parts of the region the disease is endemic that effects production with the pastoralists with an incidence >30% of their livestock production system. In a study to determine the status of anthrax in pastoralists in three selected area of Middle-Shebelle: Jawhar, Qalimow, and Aaden yabaal with Forty two herds were also used. The three selected area of the study were the most livestock populations in Middle-Shebelle. A structured Questionnaire was used to capture pastoralists Knowledge, Attitude and Their Practice with regards of Anthrax Disease. A cross-sectional survey was conducted contemporaneously with focus group discussions (FGDs) and key Informant interviews (KIIs) between May 2020 and April 2021. A Four Hundreds (4OO) of Households were visited via Personal interview. The existence of the disease per herd were estimated that 18.1% of camel herds, 34.3% of cattle herds and 47.6% of small ruminants flocks. Thus the summary of the KAP Results indicated that there was lack of Knowledge and Practice Gap among pastoralists related anthrax Disease. Generally, majority of farmers were practicing poorly in livestock community that was created the distribution of the disease. Therefore, the cattle sheep and goat herds should aware the diseases about the characterization and controlling systems through extending and trading for pastoralists and households. In addition, further assessment and control coverage with anthrax disease will highly option of essentials.
A Case of Bilateral Conjunctivitis in a Seven-Year-Old Shih-Tzu Dog Made Gede Adi Surya Saputra; Sri Kayati Widyastuti; Putu Ayu Sisyawati Putriningsih
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.87-92

Abstract

Background: Conjunctivitis is an inflammation of the conjunctiva that is common in dog, which also known as pink eyes. Conjunctivitis can be caused by infectious agents such as bacteria, chlamydia, and virus, and can also be induced by allergies or due to trauma. Bilateral infection may indicate they are caused by an infectious agent or allergy. Purpose: This study aims to provide veterinarians with insight into the treatment of conjunctivitis cases and the factors that hinder therapy. Case: A seven-year-old female Shih-Tzu dog has had eye problems for six months since April 2023. Physical examination revealed redness in the left and right eyes with mucopurulent exudate. Laboratory examination using an impression smear followed by cytology confirmed inflammation in the eye with many neutrophil cells and coccus-shaped bacteria. The dog was diagnosed with bacterial conjunctivitis with a good prognosis. Case Management: The therapy consisted of the administration of topical antibiotics in the form of Erlamycetin plus® (chloramphenicol and dexamethasone) eye drops for five days, followed by Erlamycetin® (chloramphenicol) eye ointment. Conclusion: After 12 days of therapy, there was no redness in the dog's eyes and only a small amount of exudate was observed. However, on day 14, the owner reported that exudates were observed in the dog's eyes. The therapy resulted in improvements but was not optimal because the owner did not consistently follow the recommendations.
Antimicrobial Resistance Profile of Foodborne Salmonella spp. and E. coli Isolates from Rodents and Shrews in Morogoro Municipality, Tanzania Marie Chantal Uwanyirigira; Elias Nelly Bapfakurera; Elisa Mwega; Sharadhuli Kimera; Abdul A.S. Katakweba
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.48-59

Abstract

Background: Antimicrobial resistance (AMR) is a pressing global public health issue that affects both human and animal health. Small mammals, such as rodents and shrews, represent a significant reservoir of zoonotic infections. However, there is limited documented research specifically addressing Escherichia coli and Salmonella spp. resistant strains from small mammals. Purpose: This study was conducted to examin the occurrence of AMR E. coli and Salmonella spp. and their associated resistance genes in rodents and shrews. Methods: A total of 148 fecal samples from rodents and shrews were examined for E. coli and Salmonella spp. using culture methods and biochemical tests. Fifty isolates were subjected to antibiotic susceptibility testing using the disk diffusion method on Muller-Hinton agar. Genotypic analysis by PCR was used to confirm the bacterial strains and their resistance pattern genes. Results: Two bacteria were confirmed in 57 (38.5%) of the fecal samples. Specifically, E. coli and Salmonella spp. were identified in 54 (36.5%) and 3 (2%) fecal samples, respectively. Antimicrobial susceptibility testing revealed significant resistance in Rattus rattus. Molecular analysis confirmed that Salmonella spp.  carry a larger proportion of resistance genes than E. coli. Specific resistance genes, including Sul1 and Sul2, were identified, constituting 15.4% of the total resistance genes. The prevalence of β-lactamase genes was remarkable, with a higher occurrence in Salmonella spp. Conclusion: In terms of human health, the implications of rodents and shrews serving as carriers and transmitters of various pathogens were highlighted. Given the possibility of unexpected zoonoses arising from the bacteria found in this study, these findings contribute to the understanding AMR in the context of bacterial infections in animals and humans.

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