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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol 11 No 6 (2015)" : 5 Documents clear

Bakteri Endofit dari Tanaman Kehutanan sebagai Pemacu Pertumbuhan Tanaman Tomat dan Agens Pengendali Meloidogyne sp. Abdul Munif; Arif Rafi Wibowo; Elis Nina Herliyana
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Meloidogyne sp. is one of the main constraints of tomato production in Indonesia. Endophytic bacteria may be considered as biocontrol agents for controlling Meloidogyne sp. The objective of this study was to isolate endophytic bacteria from forestry plants and to evaluate its potential for controling Meloidogyne sp. on tomato. Endophytic bacteria were isolated from roots of mahoni (Swietenia mahogany), trambesi (Albizia saman), gaharu (Aquilaria malacensis), and meranti (Shorea sp.). Isolation of bacterial endophytes from plant tissue was conducted using surface sterilization method with 70% alcohol, 3% NaOCl and sterile water on medium trypsic soy agar. Endophytic bacteria was separated and purified based on shape and color of the colony. A total of 33 isolates of endophytic bacteria were isolated from roots of mahoni (11 isolates), trambesi (5 isolates), gaharu (7 isolates), and meranti (10 isolates). The bacteria was tested for the hypersensitivity reaction on tobacco plants and the result showed that 22 isolates did not cause necrosis, indicated they are not pathogenic. Ten isolates of endophytic bacteria was selected for further experiment, i.e. to evaluate their potential as biocontrol agent for Meloidogyne sp. and as growth promotor for tomato plants. The experiment was conducted in the screenhouse using seed treatment. The result showed that two isolates of endophytic bacteria, i.e. MSJ1H and AGS1F were able to increase the growth of tomato plants up to 60% and reduce the number of root knot caused by Meloidogyne sp. Endophytic bacteria isolated from forestry plants have the potential as a biocontrol agents to plant parasitic nematode Meloidogyne sp.

Identifikasi Gen elF4G asal Oryza rufipogon pada Padi Varietas Inpari HDB dan Inpari Blas Ifa Manzila; Tri Puji Priyatno
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Wild rice accession Oryza rufipogon is known as a source of tungro resistance genes and has been used to develop tungro resistant varieties, especially against rice tungro spherical virus (RTSV). However the genes have not been identified yet. Previously, an eukaryotic Translation Initiation Factor (eIF4G) was identified as a putative gene associated with recessive resistance gene against RTSV in Utri Merah variety. The research was aimed to detect and identify the eIF4G gene on rice var.Inpari HDB and var. Inpari Blas using specific primer that was designed based on Utri Merah eIF4G gene sequences. The resistance respons of both Inpari varieties and O.rufipogon against 3 tungro virus isolates were conducted in the green house trial. Assays were done based on international rice testing nursery according to IRRI. The eIF4G gene is amplified by PCR and the amplicon was directly sequenced, then analysed in silico. The results showed that all 3 varieties are classified as resistant against tungro virus isolates. PCR was successfully amplified the eIF4G gene with size ~300 bp in both of varieties and their parent O. rufipogon. The nucleotides homology of eIF4G among those 2 varieties and O. rufipogon is up to 100%, while the homology to Utri Merah was 93%. There were 4 nucleotides deletion and 16 nucleotides differences between Utri Merah and those 2 varieties and O. rufipogon, respectively. Those nucleotide differences lead to deletion of 1 amino acid and 4 amino acids different between both Inpari varieties and O.rufipogon in comparison with corresponding amino acid in Utri Merah.

Penghambatan Fusarium oxysporum oleh Kultur Filtrat Bakteri Endofit dari Tanaman Kedelai secara in Vitro Novi Malinda; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed borne pathogen play an important role as source of inoculum for disease incidence in the field and it becomes a major constraint in certified seed production. Research was conducted to isolate potential endophytic bacteria from soybean plants and evaluate its culture filtrate for inhibition effect of seedborne fungi on soybean seed, i.e. Fusarium oxysporum. The result showed that out of forty eight endophytic bacteria isolates that were nonpathogenic, there were three potential isolates that can inhibit the growth of F. oxysporum, i.e. EDA 3, EBA 6, and EBA 7 with percent inhibition of 60.14%, 57.69%, and 57.08%, respectively. The filtrate culture of EBA 7 showed the highest inhibition (34.88%) by in vitro test. Therefore, those three isolates of endophytic bacteria might be used as biocontrol agent to inhibit the growth of F. oxysporum.

Identifikasi Virus Penyebab Penyakit Kerdil pada Tanaman Padi di Sukamandi, Jawa Barat Amelia Feryna Bulan Dini; I Wayan Winasa; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral diseases on rice is an important constraint for rice production in Indonesia. Research was conducted to determine the incidence of stunting disease in Sukamandi area at Subang regency (West Java), to identify the virus associated with the disease using RT-PCR method, and to analyze coat protein gene sequences. Field observation during growing period on November– December 2014 indicated that the incidence of stunting disease was low (0.01-10.52%). Grassy stunt and ragged stunt symptoms was observed in the fields. Spesific DNA fragments of coat protein gene of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) was successfully amplified using specific primers. Nucleotide sequence analysis showed that RRSV and RGSV isolates from Subang has the highest homology with RRSV isolates from Vietnam, Philipines and Thailand (97.1 %) and RGSV isolate from Longan, Vietnam (95.8%), respectively.

Eksplorasi Cendawan Endofit Asal Padi Sawah sebagai Agens Pengendali Penyakit Blas pada Padi Sawah Irwanto Sucipto; Abdul Munif; Yadi Suryadi; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Blast disease (Pyricularia oryzae) in Indonesia is initially known to cause problem on upland rice, but since 2000’s blast disease was also reported occurred on lowland rice. Application of endophytic fungi is very potential to be used as disease control method. This study was conducted to isolates endophytic fungi from lowland rice, and determine its capability to reduce blast disease severity. Isolation of endophytic fungi was done from root, tiller, and the leaves of lowland rice. Kencana Bali variety was used for in vivo inhibition test due to its most susceptible response against P. oryzae. Forty seven endophytic fungi isolates were obtained from Bogor, Sukabumi and Blitar. Based on colony morphology, endophytic fungi can be differentiated into 9 morphotype. Four out of fourteen endophytic fungi showed antibiosis activity in in vitro inhibition test against P. oryzae. Inhibition test conducted on Kencana Bali variety in the green house showed that four isolates was able to suppress blast disease development by 30-70%.

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