Garuda - Garba Rujukan Digital

Deteksi dan Identifikasi Cendawan Terbawa Benih Brassicaceae Anthoni Sulthan Harahap; Titiek Siti Yuliani; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed quality is very critical in agricultural production, especially to gain high yield and reduce disease problems in the field. New diseases or pathogens is potentially entering a country through seed movement by import activity. This study aimed to detect and identify seed-borne fungi from Brassicaceae seeds imported from the United States and Malaysia. Seeds were incubated on 5 sheets of wet blotting paper at a temperature of 27–30 °C for 14 days following surface sterilization. Each fungus that grows on the seed was isolated on potato dextrose agar and malt extract agar for further morphological identification. The three fungi most commonly found either on the seed with or without surface-sterilization were Aspergillus flavus, Curvularia lunata and A. niger. All of the fungi were a potential pathogen in the family Brassicaceae seeds and seedlings. Important pathogen in Brassicaceae crops, i.e. Phoma lingam was also found in small amounts and only on white pak choy seeds.

Penghambatan Fusarium oxysporum oleh Kultur Filtrat Bakteri Endofit dari Tanaman Kedelai secara in Vitro Novi Malinda; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed borne pathogen play an important role as source of inoculum for disease incidence in the field and it becomes a major constraint in certified seed production. Research was conducted to isolate potential endophytic bacteria from soybean plants and evaluate its culture filtrate for inhibition effect of seedborne fungi on soybean seed, i.e. Fusarium oxysporum. The result showed that out of forty eight endophytic bacteria isolates that were nonpathogenic, there were three potential isolates that can inhibit the growth of F. oxysporum, i.e. EDA 3, EBA 6, and EBA 7 with percent inhibition of 60.14%, 57.69%, and 57.08%, respectively. The filtrate culture of EBA 7 showed the highest inhibition (34.88%) by in vitro test. Therefore, those three isolates of endophytic bacteria might be used as biocontrol agent to inhibit the growth of F. oxysporum.

Potensi Metabolit Sekunder Cendawan Endofit Tanaman Cabai sebagai Penghambat Fusarium sp. Patogen Asal Biji Secara in Vitro Dewi Novina Sukapiring; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 12 No 1 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic fungi was known as controlling agents to pathogenic fungi, including Fusarium oxysporum. This research was aimed to select endophytic fungi from pepper which produced secondary metabolites and have beneficial effect in controlling seed borne pathogen especially Fusarium sp. Four isolates was obtained, i.e. CECL 19, CECL 28, CECL 38, and CECL 40; and further examined in 3 media fermentation, i.e. yeast glucose broth, potato dextrose broth, and potato dextrose yeast broth. Metabolites of endophytic fungi was tested in vitro for its inhibition effect on the growth of Fusarium sp. The result showed that the type of fermentation medium was significantly determining the ability of endophytic fungi in inhibiting the growth of Fusarium sp. Medium PDA and DEC was determined as the best medium to optimize metabolite production of CECL 28 and CECL 18, respectively. Metabolite compound produced by CECL 28 has been effective to inhibited Fusarium sp.

Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus Siti Hardiyanti; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Rigidoporus lignosus is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was conducted to isolate endophytic and rhizospheric microbes, and to study their ability to inhibit growth of R. lignosus. Research consisted of isolation of endophytic and rhizospheric microbes, pathogenicity test, in vitro and in vivo assays, growth promotion assays, and identification. There were 99 isolates of bacteria and 18 isolates of fungi isolated from the root and rhizosphere of rubber trees. In vitro and in vivo assay showed that 2 bacterial isolates, i.e. endophytic bacteria ME8, and rhizospheric bacteria MR3; and 3 fungal isolates, i.e. endophytic fungi CB8, CB6, and CL3 were able to inhibit the growth of R. lignosus. Endophytic bacteria ME8 showed the ability of solibilizing phosphate and fixing nitrogen. Rhizospheric bacteria MR3 showed the ability of solubilizing phosphate. The isolates CB6 and CL3 were very similar with Chaetomium sp. and Penicillium sp., respectively based on morphological characters; while CB8 was identified as mycelial sterile. Based on 16S rRNA sequences, endophytic bacterium ME8 and rhizospheric bacteria ME3 were identified as Bacillus siamensis B268 and B. amylolyquefaciens BCRh10, respectively. Endophytic and rhizospheric microbes isolated from rubber trees has the potency as biocontrol agents of R. lignosus.

Urban pests like cockroaches, mosquitoes, and flies can transmit human diseases that cause health problems in the society. Currently, mosquito is main insect that transmit very deadly diseases, such as dengue fever, cikungunya, and malaria. Prevention of those diseases is generally focused on controlling the vectors in various ways, such as fogging, the use home pesticides, sanitation, use of mosquito repellents, electric rackets, and other traditional ways developed by the community (mosquito Titiek Siti Yuliani; Hermanu Triwidodo; Kooswardhono Mudikdjo; Nurmala K. Panjaitan; Sjafrida Manuwoto
Forum Pasca Sarjana Vol. 34 No. 3 (2011): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Urban pests like cockroaches, mosquitoes, and flies can transmit human diseases that cause health problems in the society. Currently, mosquito is main insect that transmit very deadly diseases, such as dengue fever, cikungunya, and malaria. Prevention of those diseases is generally focused on controlling the vectors in various ways, such as fogging, the use home pesticides, sanitation, use of mosquito repellents, electric rackets, and other traditional ways developed by the community (mosquito net, gauze, water). The purpose of this study was to describe the relationship between characteristics of peoples live in Jakarta (based on age, sex, education, and income level) to their behavior in using home pesticides and to know the impact of those pesticides to peoples and pets live in their houses. Research sites were purposively directed to the areas that were appropriate with the substances and purposes of this study. Interviewed was conducted to the respondents (housewives) and inspection of their houses were also conducted to classify their houses as clean, moderately clean, and dirty or slum. Based on a survey to 155 respondents, it was revealed that respondent characteristics as well as their house conditions influenced the behavior of those respondents in choosing home pesticides. Majority of housewives (28.21%) use liquid pesticides which were contains the active ingredient of cypermethrin 0.4 g/l, imiprotrin 0.32 g/l, and transflutrin 0.2 g/l. In addition, 47.26% of them ignored the instruction written on the label in using those pesticides. Awareness of those respondents was still low in terms of dumping the package of pesticides; 96.64% of respondents dumped pesticides to the dumpster near their houses. Commercial advertencies from electronic media, especially TV, influence half of the respondents (52.74%) in choosing home pesticides used. In general, it can be summarized that the awareness of respondents in using home pesticides were still low. Most housewives did not know the danger of those chemicals and how to use it properly. Only 38.46% of them used non-chemical methods, such as insect net. Key words: home pesticides, active ingredient, urban pest, housewife, the environment

History of Sheath Rot Disease in Indonesia and Disease Severity in Two Rice Production Centres of West Java Khoirotul Afifah; Suryo Wiyono; Titiek Siti Yuliani; Baskoro Sugeng Wibowo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47665

The observation conducted from 2000 to 2018 found that rice sheath rot has become an emerging disease in several regions. The disease was able to reduce quality and quantity of rice yield. This research objective was to study the history and status of rice sheath rot caused by Sarocladium oryzae. Data was obtained from literature study, farmer interview, and field survey. Data were used to calculate disease incidence followed by visual observation of the symptoms caused by this disease. Identification of sheath rot pathogen was done using microscopic and molecular techniques using specific primers. Rice sheath rot was first reported in Indonesia as a minor disease in 1987 and has only currently become an important disease. This fungus generally causes rice sheath rot in Karawang and Cianjur Regency. Disease incidence of rice sheath rot was 12.56% without considering the varieties at all generative growth stages. Average disease incidence on all rice varieties observed was 12.64%, except for IR 42 (0%). The level of rice plants damage due to rice sheath rot in Cianjur was higher than Karawang with average disease incidence of 11.58% and 9.27%, respectively. Rice sheath rot symptoms have often been found in the last 10 years with average level of damage of 3%‒18% and yield loss of 10%-15%. This disease has proven to be important and has become a challenge in rice cultivation. The fungus that infect rice plants in Karawang and Cianjur had different morphotypes, namely KP, KP2, KP3, PW3, and PW03. The amplification results showed that all fungal isolates were S. oryzae.

PESTISIDA RUMAH TANGGA UNTUK PENGENDALIAN HAMA PERMUKIMAN PADA RUMAH TANGGA Titiek Siti Yuliani; Hermanu Triwidodo; Kooswardhono Mudikdjo; Nurmala K Pandjaitan; Syafrida Manuwoto
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 1 No. 2 (2011): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.1.2.73

Pesticides have become public’s need for most of Indonesian people, especially those who live in urban areas. This is due to pesticide application is the only way used by those community to control urban pest population. Unfortunately, that choice has bad impact to human health and the environment. The objective of the study was determine the factors that influence people's behavior in choosing the type of pesticides and formulation, to classify home pesticides toxicity used by the public to urban pests control, the impact of pesticides to human health and proper application based instruction manual written on the label. Data assessment revealed that peoples were very annoyed with the presence of urban pest, such as mosquitoes, ants, cockroaches, and flies. Although ants were more frequently found with higher population level, but people felt that the most disturbing one was mosquito, because they were very worry about dengue fever. To control these pests, pesticides with active ingredients cypermethrin, imiprothrin, and tansfluthrin were the most widely used by the community. Most people used pesticides with the toxicity level classified as Ib – III, according to WHO toxicity classification. The impact of pesticide exposures to human health were/ the symptoms poisoning or symptoms similar to poisoning. As much as 44.5% of people surveyed showed the symptom related to respiratory problems, 25 % of people experienced dizziness, 2.5% experienced itching on the skin, 12.5% experienced nausea and vomiting, and 5.36% had fainted. Survey was also found that 52.35% of respondents read pesticide label before use it, meanwhile 61.90% of respondents did not use pesticide properly, the later found in those who read the label or those who didn’t. Keyword: home pesticides, active ingredient, urban pest, and housewife, environmental

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search