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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol 9 No 1 (2013)" : 5 Documents clear

Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida Halimah Halimah; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extracted from female nematodes and amplified using PCR with species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (M. hapla, M. chitwoodi, and M. fallax). PCR product were sequenced without cloning. Based on nucleotide sequences, two species RKN were found associated with branched tuber disease of carrot in Agropolitan Cianjur, i.e M. javanica and M. hapla. Phylogenetic analysis showed that M. javanica from Cianjur was closely related to RKN from China with the homology level of 91.9%, whereas nucleotide sequence of M. hapla from Cianjur had high homology level (99.4% to 100%) with isolates from Swiss, USA, UK, and China.Key words: Meloidogyne hapla, M. javanica, phylogenetic, root-knot nematode

Kemampuan Aktinomiset Menghambat Pertumbuhan Sclerotium rolfsii dan Pembiakannya pada Medium Serbuk Gergaji Nurul Widyanti; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Antagonistics microorganisms, such as actinomycetes, are potential biological control agents for plant diseases. Mass culture of actinomycetes to provide enough inoculum for field application is sometimes costly. Therefore alternative medium with simple preparation and relatively less costly is required for mass culture of actinomycetes. This research was conducted to evaluate the ability of 4 actinomycetes isolates, i.e. APS 7, APS 9, APS 12, and ATS 5, in suppressing growth of Sclerotium rolfsii in vitro and to assess the growth of actinomycetes in alternatif medium containing organic wastes, i.e sawdust and bran. Among 4 tested isolates of actinomycetes only APS 7 showed its potency as biocontrol agents for S. rolfsii with growth suppression rate of 91.73% after 3 weeks incubation. In general, both sawdust and bran has the potency as culture medium for APS 7. After 8 weeks incubation period the average population of actinomycetes grew in culture medium containing sawdust and bran was 335 x 106 cfu/g and 331 x 106 cfu/g, respectively.Key words: biocontrol agents, bran, organic waste

Pengendalian Penyakit Hawar Daun Phytophthora pada Bibit Kakao dengan Trichoderma asperellum Asti Irawanti Azis; Ade Rosmana; Vien Sartika Dewi
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Phytophthora leaf blight caused by Phytophthora palmivora is one constraint in cacao seedling in Indonesia. Generally, synthetic fungicides were applied to control this disease but its negative impact is becoming obvious recently. Research was conducted to evaluate the effectiveness of two Trichoderma asperellum isolates, ARBT-1 and ART-4, to suppress disease development. The two isolates were applied through seed treatment with spore concentrations of 104 mL-1, 105 mL-1, and 106 mL-1. The results showed that disease incidence on seedling treated with different spore concentrations of ARBT-1 was 39.0%, 23.6%, and 21.8%, respectively and those with ART-4 was 30.6%, 25.7%, and 30.8%, respectively, whereas disease incidence reached 90.3% for control at 21 days after inoculation of the pathogen. Observations on cocoa leaf tissues showed that the two isolates were found in leaf tissue, indicating that T. asperellum is an endophyte.Key words: antagonistic fungi, seedling, seed treatment

Penularan Fitoplasma Sapu pada Tanaman Kacang Tanah oleh Serangga Vektor Orosius argentatus dan Deteksi Molekuler dengan Teknik PCR Tatit Sastrini; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Witches’ broom disease caused by phytoplasma is a very serious disease on peanut (Arachis hypogaea) which may potentially lead to high yield loss. Insects are the most important agents of phytoplasma transmission in the field. The objective of this research was to examine the potential role of leafhoppers species as insect vector of phytoplasma and to determine their transmission characteristic. Two species of leafhopper i.e. O. argentatus and Empoasca sp. (both belong to Hemiptera: Cicadellidae) were chosen for this study. The methodology involved were transmission study of phytoplasma by O. argentatus and Empoasca sp., and molecular detection of phytoplasma by PCR technique to confirm the association of pathogen, insect vector and symptomatic plants. The result showed that specific symptom was observed when using O. argentatus in the transmission study with number of insect as low as 1 insect per plant, whereas Empoasca sp. was not able to transmit the disease. Incubation period of phytoplasma in the host plant was affected by the number of insect, i.e. the more insect vector the shortest incubation period. The phytoplasma was successfully detected using P1/P7 primer in symptomatic plants as well as in the insect vector.Key words: Empoasca sp., leafhoppers, polymerase chain reaction

Virulensi Isolat Rice tungro virus dari Beberapa Daerah Endemis Tungro di Indonesia Suprihanto Suprihanto; Endang Nurhayati; Jumanto Harjosudarmo
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The research was conducted to distinguish the virulence of Rice tungro virus (RTV) isolates from several tungro disease endemic areas in Indonesia based on their response in differential cultivars of rice. Eight isolates of RTV were collected from several tungro endemic regions in Indonesia i.e. Bali (Denpasar), South Kalimantan (Tanah Laut), South Sulawesi (Lanrang), West Java (Subang and Bogor), Central Java (Solo), East Java (Jember), and West Nusa Tenggara (Mataram). The isolates were maintained on TN1 rice cultivar in greenhouse by successive transfers via Nephotettix virescens. Each isolate was subsequently passed on to FK135 and TN1 rice cultivars. Four week after inoculation, plants responses were observed to compare their symptom and infectivity on the differential cultivars. The results showed that the level of virulence of RTV isolate from tungro disease endemic areas in Indonesia can be classified into 3 levels. The highest virulence were RTV isolates from West Jawa, the moderate level of virulence were RTV isolates from East Java, South Kalimantan, South Sulawesi, and West Nusa Tenggara, the low level of virulence were RTV isolates from Bali and Central Java.Key words: differential cultivar, Nephotettix virescens, rice, tungro diseases

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