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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol 9 No 4 (2013)" : 5 Documents clear

Kualitas Fisik, Populasi Aspergillus flavus, dan Kandungan Aflatoksin B1 pada Biji Kacang Tanah Mentah Okky Setyawati Dharmaputra; Santi Ambarwati1; Ina Retnowati; Amanda Windyarani
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The objective of this study was to investigate the physical quality, Aspergillus flavus population and aflatoxin B1 content of raw peanut kernels collected from retailers in two traditional markets (pasar Anyar and pasar Bogor) in the city of Bogor in August 2009. The number of samples collected from pasar Anyar and pasar Bogor was 14 and 12, respectively. Physical quality of kernels was determined based on the percentage of intact, shriveled and damaged kernels. Damaged kernels consisted of broken and damaged kernels caused by insect or fungal attack. Aspergillus flavus population was determined using serial dilution method followed by pour plating method on aspergillus flavus and parasiticus agar. Aflatoxin B1 content was determined using thin layer chromatography method. The results showed, that the mean of percentage of intact, shriveled and damaged kernels of peanuts collected from pasar Anyar was 70.6, 12.3 and 17.1%, respectively; while that of collected from pasar Bogor was 60.2, 12.7 and 27.1%, respectively. The mean of A. flavus populations in peanuts collected from pasar Anyar and pasar Bogor was 8194 cfu g-1 and 983 cfu g-1, respectively. The mean of aflatoxin B1 content in peanuts collected from pasar Anyar and pasar Bogor was 2.0 ppb and 91.4 ppb, respectively. The percentage of peanut samples containing aflatoxin B1 more than 15 ppb and collected from pasar Anyar and pasar Bogor was 7.1% and 25%, respectively.

Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using reverse transcription polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.

Penggunaan Bakteriofag untuk Kit Detektor Patogen Hawar Bakteri Kedelai Nurul Rama Dhany; Hardian Susilo Addy; Wiwiek Sri Wahyuni
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial blight disease on soybean caused by Pseudomonas syringae pv. glycinea is an important factor causing yield loss in Indonesia. Bacteriophage isolated from irrigation water around the soybean field can be used as indicator for the presence of phytopathogenic bacteria. The objectives of this research was to obtain suitable composition of detector materials to detect P. syringae pv. glycinea using bacteriophage. Composition of detector kit contains of nutrient broth medium with 0.1% of bromothymol blue, 10 g talk and 1 g CMC which will be rubbed on to the detector paper and caused green colour development (pH ± 7) when the paper was dipped on to bacteria suspension, added by a drop of bacteriophage suspension and incubated for 24 hour, the colour will be changed. Yellow color indicated growth activity of P. syringae pv. glycinea where as blue colour indicated suppression of P. syringae pv. glycinea.

Ganoderma boninense Penyebab Penyakit Busuk Batang Atas Kelapa Sawit Agus Susanto; Agus Eko Prasetyo; Hari Priwiratama; Sri Wening; Surianto Surianto
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Basal stem rot disease (BSR) caused by Ganoderma boninense is the major pathogen of oil palm in Indonesia and Malaysia. Sometimes in the same location, especially in the peatland, upper stem rot (USR) disease is also found. This research was carried out to identify the pathogen of upper stem rot by molecular approach. Fruiting bodies of fungus were collected from 3 plants with upper stem rot symptoms and 2 plants with basal stem rot symptoms from Labuhan Batu North Sumatra and Kampar Riau. Results showed that all of the fruiting body samples taken from plants with upper stem rot and basal stem rot symptoms were G. boninense.

Tomato yellow leaf curl Kanchanaburi virus Penyebab Penyakit Mosaik Kuning pada Tanaman Terung di Jawa Tega Kintasari; Dwi Wiyati Nurul Septariani; Sri Sulandari; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow and yellow mosaic symptoms was observed on eggplant (Solanum melongena) around Bogor, Bandung, Pati, Rembang, and Bantul during a survey in early 2013. Polymerase chain reaction based detection was carried out using total DNA isolated from symptomatic leaf samples and a pair of Begomovirus-universal primers, SPG1/SPG2. The expected size (~900 bp) amplicon was detected from all five symptomatic samples, indicating the presence of Begomovirus infection. Each amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) sequence. This is the first report on TYLCKaV infection on eggplant in Indonesia.

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