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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrokompleks
Sari Nurulita
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Infeksi Papaya ringspot virus pada Tanaman Pepaya di Provinsi Nanggroe Aceh Darussalam Sri Hendrastuti Hidayat; Sari Nurulita; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.156 KB) | DOI: 10.14692/jfi.8.6.184

Abstract

Severe mosaic symptoms was observed on papaya plants in Meusa village, Kutablang sub district, Bireun district and Lambaro Teunom village, Lembah Seulawah sub district, Aceh Besar district. Systemic mosaic was found in leaves, stem, twig, and fruit. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the virus causing mosaic symptoms using universal primer for Potyvirus. Amplification of 320 bp DNA fragment was successfully obtained from leaves and fruits bearing severe mosaic symptoms. Nucleotide sequencing was proceeded using the DNA amplicon as the template. Further sequence analysis indicated that Potyvirus isolates from papaya in Aceh show the highest homology (92.7% to 94.7%) with Papaya ringspot virus (PRSV) from Philippines, Thailand, and Indonesia. This is the first report on PRSV infection on papaya in Nanggroe Aceh Darussalam province.   Key words: Potyvirus, RT-PCR, sequencing 
Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (809.939 KB) | DOI: 10.14692/jfi.9.4.107

Abstract

Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using reverse transcription polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.
Kloning Gen Transcriptional Activator Protein dan Replication-Associated Protein dari Squash leaf curl China virus Penyebab Penyakit Daun Kuning pada Tanaman Mentimun Ni Nengah Putri Adnyani; I Dewa Made Putra Wiratama; I Gede Rai Maya Temaja; Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.623 KB) | DOI: 10.14692/jfi.14.1.1

Abstract

Yellow disease on Cucurbitaceae caused by Squash leaf curl China virus (SLCCNV; Begomovirus) is an important disease in many countries. Typical yellow leaf curl symptoms was found on cucumber in Tabanan, Bali. The objective of this research is to clone Transcriptional Activator Protein (TrAp) and Replication-Associated Protein (Rep) of SLCCNV Indonesian isolate. Universal primer for Begomovirus, i.e. SPG1/SPG2 was used to amplify DNA fragment of Begomovirus which cover the TrAp and Rep gene. The amplicon of ± 912 bp in size was then cloned into pTZ57R/T plasmid vector by TA-cloning method to obtain plasmid recombinant pTZ-SLCCNV. The plasmid recombinant was successfully confirmed by PCR colony and DNA sequencing. The nucleotide sequences analysis of plasmid recombinant showed highest homology (98%) of the corresponding gene to pumpkin isolate of SLCCNV from Malaysia.
Molecular Characterization of Begomovirus Infecting Yard Long Bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia Sari Nurulita; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; John Thomas
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 22 No. 1 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.401

Abstract

Begomovirus was identified as one of the causal agents causing yellow mosaic disease on yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in Java. Research was conducted to characterize the virus based on its nucleotide sequences.  Research was started by field survey to collect leaf samples, followed by virus detection using polymerase chain reaction and analysis of virus sequences.  Samples from Tegal, Klaten, Magelang, Cirebon, Subang, and Bogor were positively infected by Begomovirus based on specific viral DNA amplification.  Sequence analysis indicated that Begomovirus infecting yard long bean belongs to the same group with Mungbean yellow mosaic India virus (MYMIV) from Bangladesh, India, Pakistan, and Nepal.  Further analysis showed the conserved region of Begomovirus around Common Region, i.e. “TATA box” Sequence, Hair Pin Loop Structure, Repetitive Sequence, and the Conserved Nonanucleotide Sequence TAATATTAC.Key words: common region, DNA sequencing, ELISA, MYMIV, PCR 
Pengembangan Deteksi Spesifik untuk Mungbean Yellow Mosaic India Virus yang Menginfeksi Kacang Panjang di Jawa, Indonesia Nurulita, Sari; Mutaqin, Kikin; Hidayat, Sri
Jurnal Fitopatologi Indonesia Vol 19 No 2 (2023): Maret 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.2.83-88

Abstract

Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV.
First Report of Celery mosaic virus Infecting Celery (Apium graveolens) in Indonesia: Laporan Pertama Celery mosaic virus yang Menginfeksi Seledri (Apium graveolens) di Indonesia Makyorukty, Dhayanti; Damaiyanti, Kartika Catur; Sianipar, Theresia Aprilia; Nurulita, Sari
Jurnal Fitopatologi Indonesia Vol 20 No 3 (2024): Mei 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.3.109-114

Abstract

Celery mosaic virus (CeMV), member of genus Potyvirus, is reported for the first time in Indonesia, from celery plants (Apium graveolens) in a vegetable field at Berastagi, North Sumatera Province. The plants possessed mosaic and vein clearing symptoms on the leaves as typical of CeMV infection. Virus incidence was confirmed by RT-PCR using degenerate potyvirus primer which amplified partial coat protein and 3’-UTR of the viral genome. Phylogenetic tree analysis placed Indonesian CeMV isolates in one separated clade within CeMV group and shared 96.5%–96.7% nucleotide identity with exemplar isolate of CeMV.
Identification of Garlic Viruses Associated with Seed Bulbs and Consumption Bulbs from Several Locations in Indonesia Nurulita, Sari; Mawarni, Sofi; Hidayat, Sri Hendrastuti
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.733-743

Abstract

Virus infection is one of the major constraints in garlic production since the viruses are readily accumulated on vegetative propagation material (bulbs). This research aimed to detect garlic common latent virus (GCLV), shallot latent virus (SLV), onion yellow dwarf virus (OYDV), and leek yellow stripe virus (LYSV) infecting local garlic as seed bulb and imported garlic as consumption bulb. Seed bulb samples were obtained from seed breeders in several garlic growing centers in Indonesia. In contrast, consumption bulb samples were obtained from plant quarantine warehouses and three local markets in Bogor. Some bulb samples were used for morphological observations, and some were germinated in the laboratory until the leaves emerged. Leaves were collected for virus detection by RT-PCR using specific primers for GCLV, SLV, OYDV, and LYSV. Seed and consumption bulbs have differences in their morphological characteristics, especially in the type of neck hardness and the size of the bulb diameter. OYDV and LYSV infections were successfully detected in seed and consumption bulbs, while SLV was only found in consumption bulbs. Nucleotide sequence analysis showed that SLV from consumption bulbs formed one group, GCLV from seed bulbs formed one group, while OYDV and LYSV from seed and consumption bulbs were in different groups, indicating that the viruses came from different strains. Further research through high-throughput detection methods and providing virus-free planting material are needed to anticipate the spread of new strains of garlic viruses in Indonesia.
Molecular Evidence Points to Strong Resemblance in the Parasitoid Species of Rice and Cogongrass Gall Midges, Platygaster spp. (Hymenoptera: Platygasteridae) Hidayat, Purnama; Lestari, Yuliana Ayu; Nurulita, Sari
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.683-692

Abstract

The rice gall midge, Orseolia oryzae, and the cogongrass gall midge, O. javanica, cause gall formation on rice and cogongrass (alang-alang) (Imperata cylindrica). Two different species parasitize these two gall midges but closely related platygasterids, Platygaster oryzae on the rice gall midge and P. orseoliae on the cogongrass gall midge. Both the gall midges and their parasitoids are often found in the adjacent area, raising a question about the relationship between the two gall midges and their parasitoids. This research aims to study the molecular identity of the rice and cogongrass gall midges, along with their platygasterid parasitoids, based on partial sequences of the mtCOI gene. Samples were collected from rice and cogongrass in the adjacent area in Cianjur, West Java Province, and a rice field with no cogongrass in Bogor, West Java Province. Successful DNA amplification was achieved using universal primers for mtCOI. Nucleotide sequencing analysis revealed that the rice gall from Bogor and Cianjur shared 100% similarity and 93.2-99.3% similarity with the rice gall from other countries. Notably, the parasitoids P. oryzae collected from rice in Bogor and Cianjur shared 97.2% similarity with P. orseoliae collected from cogongrass in Cianjur. These findings suggest that the platygaster parasitoids associated with the rice gall and the cogongrass gall midges are identical, serving as potential natural enemies for both pests. This study represents the first molecular identification report of rice and cogongrass gall midges and their platygasterid parasitoids from Java Island, Indonesia.
Identifikasi kutu daun yang berasosiasi dengan tanaman jeruk Syarni, Elisa; Taufik, Muhammad Taufik; Yusuf, Dewi Nurhayati; Nurulita, Sari; Miftakhurohmah, Miftakhurohmah; Botek, Muhammad; HS, Gusnawaty; Hikamwaty, Aisyah
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.918

Abstract

Aphids are polyphagous insects commonly found on various plants, including citrus plants. The association between aphids and citrus plants plays a role as pests and vectors of citrus plant diseases, particularly Citrus tristeza virus (CTV). Various aphid species have been reported as CTV vectors, including Aphis gossypii, Toxoptera aurantii, and T. citricidus. So far, no information has been on aphid species associated with citrus plants in Southeast Sulawesi. Therefore, this study aimed to identify aphid species associated with citrus plants. The research method used was observation and collection of aphids found colonizing citrus plants. The collected aphids were then brought to the laboratory for detection using the polymerase chain reaction (PCR) technique. The study results showed that the aphid species associated with citrus plants was Aphis citricidus. The PCR technique successfully detected the genome of aphid vector samples from Konawe and South Konawe (700 bp). This information is a fundamental basis for controlling CTV in mainland Southeast Sulawesi.
Identification of Polerovirus Infecting Chili Pepper in Java: Identifikasi Polerovirus yang Menginfeksi Tanaman Cabai di Jawa Saputra, Andri; Giyanto; Nurulita, Sari; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 21 No 1 (2025): Maret 2025 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.1-6

Abstract

Infection of polerovirus in chili peppers has been reported from various parts of the world, including Indonesia. Pepper vein yellow virus (PeVYV), a member of genus Polerovirus were reported infecting chili plants in Bali, Java, and Aceh during 2012 to 2020. Recently, symptoms of the polerovirus infection, such as yellowing leaves with vein thickening, were found in chili pepper crops in the Central Java Region, specifically in Brebes, Magelang, and Kulon Progo. Molecular detection by RT-PCR method using universal polerovirus primers O3R/PL4F, followed by sequence analysis, confirmed the presence of PeVYV on samples from Magelang and Kulon Progo. Furthermore, the isolates of PeVYV are identified as PeVYV-2, PeVYV-6, and PeVYV-8. These current incidences indicate the expansion of the Polerovirus distribution in Indonesia.
Co-Authors Botek, Muhammad Damaiyanti, Kartika Catur Gede Suastika Giyanto GUSNAWATY HS, GUSNAWATY Hidayat, Sri Hikamwaty, Aisyah I Dewa Made Putra Wiratama I G. R. M. TEMAJA John Thomas Kikin H Mutaqin Lestari, Yuliana Ayu Makyorukty, Dhayanti Mawarni, Sofi MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Ni Nengah Putri Adnyani PURNAMA HIDAYAT RR. Ella Evrita Hestiandari Safinah Surya Hakim, Safinah Surya Sianipar, Theresia Aprilia SRI HENDRASTUTI HIDAYAT Suryo Wiyono Syarni, Elisa Taufik, Muhammad Taufik Tri Wira Yuwati, Tri Wira Yusuf, Dewi Nurhayati