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Ganoderma boninense Penyebab Penyakit Busuk Batang Atas Kelapa Sawit Agus Susanto; Agus Eko Prasetyo; Hari Priwiratama; Sri Wening; Surianto Surianto
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Basal stem rot disease (BSR) caused by Ganoderma boninense is the major pathogen of oil palm in Indonesia and Malaysia. Sometimes in the same location, especially in the peatland, upper stem rot (USR) disease is also found. This research was carried out to identify the pathogen of upper stem rot by molecular approach. Fruiting bodies of fungus were collected from 3 plants with upper stem rot symptoms and 2 plants with basal stem rot symptoms from Labuhan Batu North Sumatra and Kampar Riau. Results showed that all of the fruiting body samples taken from plants with upper stem rot and basal stem rot symptoms were G. boninense.

KERAGAMAN GENETIK POPULASI E. oleifera DAN POPULASI E. guineensis x E. oleifera PADA KOLEKSI PLASMA NUTFAH PPKS Rokhana Faizah; Sri Wening; Hernawan Yuli Rahmadi; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 1 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

The oil palm species of Elaeis oleifera have some superior characters not possessed by E. guineensis species. The cross between E. oleifera x E. guineensis (E. o x E. g.) Is carried out to insert the superior character of E. oleifera into the E. guineensis species and to obtain individuals who possess a superior characteristic blend of both species. This study aims to determine the genetic diversity within and between populations of E. oleifera, as well as relationships between E. oleifera, E. guineensis and its interspecific hybrids. A total of 8 germplasm populations used were E. oleifera originating from Suriname and Brazil, E. guineensis population of Dura Deli and SP540T, and interspecific crosses of hybrid E. guineensis x E. oleifera from Colombia, hybrid E. guineensis x E. oleifera which is presumably from Colombia, as well as hybrid E. guineensis x E. oleifera from Brazil. A total of sixteen Simple Sequence Repeat (SSR) markers were used to analyze 92 individuals from 8 populations. PCoA results show that 8 Elaeis populations cluster in each group. The hybrid population of E. guineensis x E. oleifera suspected of Colombia has a very close kinship relationship with hybrid E. guineensis x E. oleifera from Colombia and clumped in the same quadrant. This suggests that the hybrid is indeed a hybrid of E. guineensis x E. oleifera from Colombia. The population of E. oleifera from Suriname showed the lowest genetic diversity, with the number of different alleles, specific alleles, heterozygosity values, and the percentages of polymorphic loci sequentially were 1.37, 0.18, 0.09, and 37.50%. While the population of interspecific interspecific crossbreeds of the E. oleifera species showed the highest genetic diversity with values of 3.81, 0.43, 0.62 and 100%.

DUGAAN GEJALA DEPRESI SILANG-DALAM DAN TINGKAT HOMOZIGOSITAS POPULASI KELAPA SAWIT HASIL PENYERBUKAN SENDIRI GENERASI KE-4 SP540T DAN GENERASI KE-5 DURA DELI Rokhana Faizah; Sri Wening; Hernawan Yuli Rahmadi; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 2 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Inbreeding is a common method used to reproduce candidate mother plant from selected parental lines for commercial seeds in Reciprocal Recurrent Selection (RRS) oil palm breeding program. However this practice may increased homozigosity level of selected population. This study concerned the level of homozygosity of SP540T fourth generations and Dura Deli Dolok Sinumbah fifth generations (3 crosses respectively) and their correlation with inbreeding depression symptoms. Polymerase Chain Reaction-Simple Sequence Repeat (PCR-SSR) with 16 markers developed for oil palm was used to analyze 327 samples. The result shows that the levels of homozigosity of SP540T fourth selfing generation were ranged between 0.44-0.84 or 0.61 in average. While the levels of homozygosity of Dura Deli fifth selfing generations were ranged between 0.60-0.93 or 0.78 in average. The homozygosity level in Dura Deli was 1.27% higher than SP540T populations. Correlation analysis showed that the higher the level of homozygosity, the higher of the inbreeding symptoms 2 observed (R =0.95).

KETELUSURAN GENETIK KETURUNAN KELAPA SAWIT (Elaeis guineensis Jacq.) MENGGUNAKAN SIMPLE SEQUENCE REPEAT (SSR) Rokhana Faizah; Sri Wening; Yurna Yenni; Sujadi Sujadi
Jurnal Penelitian Kelapa Sawit Vol 24 No 3 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Progenies traceability status becomes an important activity in the oil palm breeding program, especially to obtain propriety of individual and progenies population with their parents. This study is concerned to analyze genetic traceability based on pattern and allele size of their parent using Simple Sequence Repeat (SSR) markers. There were 90 palms from 7 progenies populations in the AD02S, AD03S Adolina and MA22S Marihat Estate PT. Perkebunan Nusantara IV used in this research. Leaf and pollen samples were used to get genomic DNA. Then, DNA genomic was amplified using multiplexing method of 8 SSR markers and fluorescence labels of 6-FAM, HEX, and NED. Fragment analysis and extracted genotype data was obtained using Gene Marker® versi 2.4.0 Soft Genetics® LLC program. Genetic traceability analysis was based on alelle segregation pattern of Mendelian Law. The results described inappropriate alleles 4 individuals from 3 progenies (N, P, and S). Those individuals were palmrow of 22-30 in the AD02S; 29-27 in the MA22S; 9-22 and 9-28 in the AD02S. Other 4 progenies showed an appropriate segregation of genotype with their parents, which are crosses number of M, Q, R, and O.

KARAKTERISASI GEN β KETOACYL-ACP SYNTHASE II (KAS II) PADA Elaeis guineensis, Elaeis oleifera, DAN HIBRIDA INTERSPESIFIK Heri Adriwan Siregar; Sri Wening; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 3 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Oil palm (Elaeis guineensis) is currently the highest edible oil-producing plant in the world. E. oleifera is another species of oil palm that is not widely cultivated due to lower productivity. Oil extracted from E. guineensis has a balance ratio of saturated to unsaturated fatty acids level, while E. oleifera has a higher unsaturated fatty acids. Long chain saturated fatty acids are more harmful for human health than unsaturated fatty acids. Gene encoding β-ketoacyl ACP synthase II (KAS II, EC 2.3.1.179) was studied, as this enzyme is responsible for biosynthesis of unsaturated fatty acids. Observation and analysis were conducted for KAS II gene and fatty acids content of E. guineensis represented by DxP Lame, DxP Langkat and CMR78 samples, E. oleifera represented by Suriname origin, and two hybrids (E. guineensis x E. oleifera (Brazil and Suriname)). The results showed E. oleifera Suriname had the highest concentration of oleic acid, followed by E. guineensis (CMR78). KAS II gene sequences alignment showed some genetic variation within E. guineensis and hybrids, while the high level of variation found between E. guineensis and E. oleifera was considered caused by species difference.

KONSTRUKSI PETA GENETIK KELAPA SAWIT (Elaeis guineensis JACQ.) PPKS KETURUNAN SP540T DENGAN MENGGUNAKAN MARKA RESTRICTION SITE ASSOCIATED DNA (RAD) Retno Diah Setiowati; Enrique Ritter; Sri Wening; Yurna Yenni; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Construction of genetic linkage on oil palm derived SP540T was established in 2012-2015 in colaboration with PPKS, Indonesia with Neiker, Spain. DNA isolation was conducted in Molecular Biology Laboratory in PPKS Marihat meanwhile RAD analysis was performed in Laboratorio Biotecnologia Vegetal, Neiker, Spain. Genetic map construction was carried out using Restriction Site Associated DNA. A total 532 RAD fragments were applied to configure 16 Linkage Groups (LGs) which represented 16 chromosomes of oil palm. The map spans in 1269.9 map unit (mU) or 79.37 mU per chromosome in average and consists of RAD fragments which could be blasted to the DNA data base to identify candidate genes. Genenic map which had been formed is a fondation of constructing an IOPRI functional map (IOPRImap) when it is integrated with phenotypic data. The IOPRImap is expected to facilitate shortening oil palm selection cycle and defining a precise oil palm breeding strategy.

KEMURNIAN KETURUNAN MENGGUNAKAN MARKA SSR SEBAGAI SISTEM KONTROL DAN SELEKSI DINI KELAPA SAWIT (Elaeis guineensis JACQ.) Rokhana Faizah; Sri Wening; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

Studi Asosiasi Genom (GWAS) Komponen Tandan Populasi Interspesifik Elaeis oleifera dan Elaeis guineensis Heri Adriwan Siregar; Edy Suprianto; Sujadi Sujadi; Hernawan Y Rahmadi; Mohamad Arif; Retno Diah Setiowati; Nanang Supena; Rokhana Faizah; Sri Wening; Yurna Yenni; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 29 No 2 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i2.144

The oil palm breeding program for the species Elaeis guineensis and the backcross Elaeis oleifera is running slowly because oil palm is an annual plant. Therefore, it is necessary to have an alternative approach that can accelerate the oil palm breeding program. The SNP (single nucleotide polymorphism) genome-wide approach was then used to study the association between 18 phenotypes of bunch component in oil palm germplasm of E. oleifera from Suriname and Brazil Coari, some interspecific hybrids and some elite progeny of E. guineensis. The genotyping by sequencing (GBS) analysis produced a total of 459 million or approximately 798 thousand reads per sample and 3,252 SNPs were eligible for 456 genotypes. Using various association models, eleven normalized phenotypic data showed significant associations with 29 SNPs. Based on the annotations, 17 SNPs were related to genes wtih certain biological functions. Three SNPs were found to be at the exon of a gene, namely SNP4416, SNP349 and SNP3865, while the other 15 SNPs were at the intragenic to a gene. Four SNPs are common SNPs in phenotypes C16:0 and C18:1 as weel as in C20 0 and C20:1. This research shows the potential of SNPs that can be used as an alternative approach to E. oleifera backcross breeding, although further research is needed for validation purposes.

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