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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 5 Documents

Search results for , issue "Vol. 8 No. 2 (2012)" : 5 Documents clear

Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique) Tuti Susanti Legiastuti; Tri Aminingsih
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta and further identification using molecular technique involving polymerase chain reaction (PCR) and DNA sequencing was performed. DNA fragments of ±500 bp were successfully amplified from 10 fungal isolates by PCR using primer pair ITS1/ITS4, but only 8 DNA sequences was obtained for further genetic analysis. Based on BLASTN analysis the endophytic fungi were identified as having the highest similarity with Pleosporaceae sp. (98%) for H1 isolate, Cercospora nicotianae (100%) for H5 isolate, ercospora piaropi (98%) for H11 isolate, Guignardia mangiferae (99%) for H16 isolate, Geomyces pannorum 95%) for H17 isolate, Diaporthe phaseoloru (99%) for H18 isolate, Dothideomycete sp. (100%) for K3 isolate, and Alternaria longissima (99%) for K10 isolate. Key words: Begomovirus, chillipepper, DNA sequencing, polymerase chain reaction

Penggunaan Bakteri Kitinolitik sebagai Agens Biokontrol Penyakit Busuk Batang oleh Rhizoctonia solani pada Tanaman Kedelai (Utilization of Chitinolitic Bacteria as Biocontrol Agent of Stem Rot Disease by Rhizoctonia solani on Soybean) Andi Khaeruni; Abdul Rahman
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Biological control of stem rot disease caused by Rhizoctonia solani on soybean using chitinolitic bacteria is one of the environmentally friendly control methods of pathogen. The study was conducted to select chitinolitic bacteria and to test their ability to suppress stem rot disease and promote plant growth of soybean. A total of ten chitinolitic bacteria were tested in vitro, and five of isolates i.e: ST21e, SS12b, ST17c, ST27d, and ST26c found to have more than 30% suppression toward R. solani. In planta test under green house condition at six weeks after planting showed that soybean plants inoculated with ST27d and ST17c isolates had significantly lower disease incidence and had increased plant height and leaf number. Both of chitinolitic bacteria isolates are promising as biocontrol agents of stem rot disease caused by R. solani of soybean.Key words: biological control, chitinolitic bacteria, Rhizoctonia solani

Ketahanan Lapangan Padi Gogo terhadap Infeksi Curvularia oryzae Muhammad Taufik; Asniah .; Syair .
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Southeast Sulawesi is known to have a wide varieties of local cultivars of upland rice. Unfortunately, resistance of those local cultivars of upland rice to Curvularia oryzae, one of the most important pathogen in upland rice cultivation, has not been reported. Resistance evaluation conducted in the field showed that local cultivars (Pae Kori, Pae Endokadia, and Pae Enggalaru) have high level of resistance, developing lines B10580E and B11580E have moderate resistance, whereas B11577E and TB368B lines were susceptible to C. oryzae infection.Key words: Curvularia oryzae, disease resistance, upland rice

Evaluasi Tiga Metode Preparasi RNA Total untuk Deteksi Turnip mosaic potyvirus dari Benih Brassica rappa dengan Reverse Transcription-Polymerase Chain Reaction Jati Adiputra; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Turnip mosaic virus (TuMV) is an important seedborne virus that infects vegetable crops, especially Brassicae group, in Indonesia. Reliable detection method having high accuracy is required to detect the virus from seeds in order to avoid disease incidence as early as possible. A study was conducted to evaluate three total RNA extraction methods from Brassica seeds for TuMV detection using reverse transcription- polymerase chain reaction. The samples used are Brassica rappa seeds and seedling germinated for 3, 5, and 7 days. Total RNA extraction methods evaluated consisted of Wylie method, Randles method, and commercial kit as comparation. Total RNA extracted using Wylie and Randles methods has high concentration but low level of purity, on the other hand total RNA obtained using commercial kit has low concentration but high level of purity. Detection of TuMV from seed was successfully carried out using Wylie and Randles methods, TuMV from 3 - day old seedlings was detected using all three methodes, TuMV from 5-day old seedlings was only detected using Randles method, whereas TuMV from 7-day old seedlings was not detected using all three methodes. Total RNA extraction method using Wylie and Randles methodes is recommended for detection of TuMV from caisin seed using RT-PCR technique. Key words: Brassica rappa seed, reverse transcription- polymerase chain reaction, Total RNA, Turnip mosaic potyvirus

Laporan Pertama tentang Penyakit Busuk Arang pada Kacang Tanah Suryo Wiyono; Widodo .
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Peanut charcoal rot disease was first observed in Bogor,West Java in June 2010, and Serang, Banten in January 2012. Infected plants in the field showed root and stem rot, discoloration of stems and/or branches. Severe infection may cause wilting of foliage then plant dried out. Based on microscopic observation Macrophomina phaseolina was constantly associated with diseased plants from those two areas. Hot temperature and low rainfall might favour disease development. This is the first report of charcoal rot disease of peanut in West Java and Banten. Key words: charcoal rot, Macrophomina phaseolina, peanut

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Jawa barat

INDONESIA