Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

3.625

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Palawija AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Palawija

TRI ASMIRA DAMAYANTI

Departemen Proteksi Tanaman, Fakultas Pertanian IPB, Jln. Kamper, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia, 16680

Author-ID : 249190

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

Published : 66 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

IDENTIFICATION AND CHARACTERIZATION OF CARMOVIRUS ON CARNATION (Dianthus caryophyllus L.) IN WEST JAVA, INDONESIA Diningsih, Erniawati; Suastika, Gede; Damayanti, Tri Asmira; Susanto, Slamet
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Carnation has a highly economic demand of cut flower in Indonesia.Â Field observations in West Java Indonesia was conducted in order to find the typical mottle symptoms that was a suspect caused by a virus disease. Identification of the virus was respectively conducted by performing ELISA test with four anti sera and characterizations held by bioassay, observing of virion particles, detecting of nucleic acid by RT-PCR and nucleotide sequencing. Total of 403 samples were collected from plants with or no virus-like symptoms. Among those all tested, 83% were found to be infected by Carnation mottle virus (CarMV), but negatively against Carnation ringspot virus (CRSV), Carnation laten virus (CLV), and Carnation vein mottle virus (CVMV) antisera. By mechanical inoculation, the virus was able to infect systemically Cenopodium quinoa and locally infect on others. However on Phalaenopsis sp and Gomprena globosa, there was symptompless found. The isometric CarMV particles size was approximately 30 nm. RT-PCR using specific primers of CP gene of CarMV successfully amplified a DNA sized 1000 bp.Â CarMV West Java Indonesian (Idn-WJ) isolates possessed the highest nucleotide and amino acid homology with CarMV from Spain and was in the same cluster with CarMV from China, Taiwan and Israel.Keyword: Dianthus caryhophyllus, RT-PCR, sequence, Tombusviridae

DETEKSI SECARA SEROLOGI DAN MOLEKULER BEBERAPA JENIS VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK TANAMAN NILAM (Pogostemon cablin Benth) MIFTAKHUROHMAH, MIFTAKHUROHMAH; SUASTIKA, GEDE; DAMAYANTI, TRI ASMIRA
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAKPenyakit mosaik pada tanaman nilam disebabkan oleh beberapajenis virus, yaitu Potyvirus, Potexvirus, Cucumber mosaic virus (CMV),dan Broad bean wilt virus 2 (BBWV2). Penelitian ini bertujuan untukmengidentifikasi secara serologi dan molekuler virus-virus yangberasosiasi dengan gejala mosaik pada nilam di KP. Manoko, KP. Cicurugdan lahan petani di Cijeruk. Sampel daun nilam baik yang menunjukkangejala mosaik atau pun tidak diambil dari setiap lokasi penanamanmasingâmasing sebanyak 30 sampel. Kejadian penyakit ditentukan melaluideteksi serologi dengan Direct-ELISA dan Indirect-ELISA terhadap sampelmenggunakan empat antiserum, yaitu CMV, Cymbidium mosaic virus(CymMV), Potyvirus, dan BBWV2. Deteksi molekuler dengan RT-PCRdilakukan untuk mengonfirmasi virus baru yang ditemukan. Hasilpenelitian menunjukkan bahwa gejala infeksi virus yang ditemukan padanilam bervariasi, yaitu mosaik lemah, mosaik kuning hijau, mosaik denganpenebalan, mosaik dengan malformasi daun, dan bintik kuning. Secaraserologi, kejadian virus pada setiap kebun bervariasi. Di KP Manoko,Potyvirus dan BBWV2 lebih dominan (100%) dibandingkan CymMV. DiKP Cicurug, kejadian Potyvirus dan CMV terlihat lebih dominan (83,3 dan80%) dibandingkan BBWV2 dan CymMV, sedangkan di Cijeruk, BBWV2lebih dominan (90%) dari Potyvirus (50%) dan CMV (13,3%). Hasil RT-PCR dengan primer degenerate BBWV, diidentifikasi BBWV2 padasampel daun nilam dari Manoko, Cicurug, dan Cijeruk, sedangkan denganprimer general Potexvirus, diidentifikasi CymMV hanya dari sampel daunnilam dari asal Manoko. Hasil penelitian ini merupakan laporan pertamatentang BBWV2 dan CymMV pada tanaman nilam di Jawa Barat yangmengindikasikan bahwa virus merupakan kendala utama pada perbenihannilam yang harus segera diatasi.Kata kunci: BBWV2, CymMV, mosaik, Pogostemon cablin Benth, PCRABSTRACTMosaic symptoms on patchouli plant are associated with severalviruses, i.e. Potyvirus, Potexvirus, CMV, and BBWV2. The objective ofthe study was to detect virus(es) associated with mosaic symptoms onpatchouli at the the patchouli seed nurseries, in Manoko, Cicurug, andCijeruk. Thirty leaf samples either showing typical symptomatic mosaic orasymptomatic were taken from each location. Serological testing byDirect-ELISA and Indirect-ELISA using four antisera namely CMV,Cymbidium mosaic virus (CymMV), Potyvirus, and BBWV2 was carriedout to test the incidence of each virus. Molecular detection by RT-PCR wasperformed to confirm the new virus(es). The results showed that symptomsof virus infection were found vary, i.e. weak mosaic, green yellow mosaic,mosaic with thickening, mosaic with leaf malformations, and yellow spot.Based on the serological detection, virus(es) incidence varied at each seednurseries. In Manoko, Potyvirus, and BBWV2 were more dominant(100%) compared with CymMV. In Cicurug, Potyvirus and CMV weremore dominat (83.3 and 80%) compared with BBWV2 and CymMV.While in Cijeruk, BBWV2 was the most dominant (90%) than Potyvirus(50%) and CMV(13.3%). Result of RT-PCR with degenerate primers pairof BBWV was succesfully identified BBWV2 from Manoko, Cicurug, andCijeruk samples, whereas by using Potexvirus general primary, CymMVwas identified only from Manoko samples. BBWV2 and CymMV werefirst reported to infect patchouli in West Java. The result indicate thatvirus(es) are the major constraint on patchouli seed that should bemanaged immediately.Key words: BBWV2, CymMV, mosaic, Pogostemon cablin Benth, PCR

UTILIZATION OF PLANT EXTRACTS TO SUPPRESS Squash Mosaic Virus INFECTION ON RIDGED GOURD (Luffa acutangula [L.Roxb]) Putri, Maharani Mustika; Damayanti, Tri Asmira
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.220108-115

Utilization of plant extracts to suppress Squash Mosaic Virus Infection on ridged gourd (Luffa acutangula [L]. Roxb).Control effort strategies against plant pathogens are necessary to be explored and evaluated its effectiveness intensively.One environmentally friendly control method is biological control by using plant extracts. It is well known that cucurbits arecultivated widely in Indonesia. However, cucurbits are susceptible plants against various pathogens, including viruses.Recently, Squash mosaic virus (SqMV) found to infect cucurbits such as ridged gourd in West Java. The research aimed toexplore and evaluate the effectiveness of plant extracts to suppress SqMV infection on the ridged gourd. Three selectedplants, namely Bougainvillea spectabilis, Mirabilis jalapa, and Celosia cristata, were tested in greenhouse trial with aspirinas a comparison. The extract was sprayed on the leaves a day before mechanical virus infection. The disease assessments andagronomic parameters were observed. The plant extract treatment showed able to prolonged the incubation period, causedmilder symptoms, reduced the virus titer, and showed better plant growth in compared with aspirin treatment or untreatedcontrol. B. spectabilis and M. jalapa crude leaf extract revealed able to suppress the SqMV infection with comparableeffectiveness with relative inhibition level of disease severity and virus titer up to 48.2% and 80.6 to 81.4%, respectively,indicating their potential as biocontrol for SqMV.

Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR Ladja, Fausiah T.; Hidayat, Sri Hendrastuti; Damayanti, Tri Asmira; Rauf, Aunu
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Virus tungro disease is a serious problem to rice crop in a certain area of rice production in Indonesia. The disease is caused by a combined infection of Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV). Both viruses were reported to infect ratoon rice plants, weeds, and wild rice. The study was conducted to detect RTBV and RTSV on some weeds. Weed samples were collected from rice fields in West Java, Bali, West Nusa Tenggara, Papua, and West Sumatera. The detection of RTBV and RTSV were carried out using Polymerase Chain Reaction (PCR) and Reverse Transcription (RT) â€“ PCR, employing coat protein gene specific primers. RTBV specific DNA fragment of ~1400 bp size was successfully amplified from various weed species including: F. miliacea, C. iria, M. vaginalis, L. adscendens, S. zeylanica, D. sanguinalis, and E. crusgalli. RTSV specific DNA fragment of ~787 bp size was successfully amplified from weed species of F. miliacea, L. octovalvis, and D. sanguinalis. RTBV or RTSV specific DNA fragment was not amplified from L. flava and P. distichum. Weed samples infected by both viruses did not show any tungro symptom. Virus detection based on molecular technique was able to determine the status of weed whether it is as an alternate host of viruses. Weeds sanitation prior to rice planting, therefore, should be considered as an integral part of virus disease management.

Severe Outbreak of a Yellow Mosaic Disease on the Yard Long Bean in Bogor, West Java TRI ASMIRA DAMAYANTI; OLUFEMI JOSEPH ALABI; RAYAPATI ADIKESAVULU NAIDU; AUNU RAUF
HAYATI Journal of Biosciences Vol. 16 No. 2 (2009): June 2009
Publisher : Bogor Agricultural University, Indonesia

During 2008 crop season, an outbreak of severe yellow mosaic disease on yard long bean (Vigna unguiculata subsp. Sesquipedalis) occurred in several farmers’ fields in West Java. Yard long bean var. Parade inoculated manually with extracts from symptomatic leaves showed the symptoms indicating the presence of virus. Symptomatic leaf samples tested positive in enzyme linked immunosorbent assay (ELISA) with antibodies to group specific Potyvirus and Cucumber mosaic virus (CMV). Total RNA derived from symptomatic leaves was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers specific to the cylindrical inclusion (CI) protein of potyviruses and CMV coat protein (CP) specific primers. Pair wise comparison of sequences obtained from cloned RT-PCR products with corresponding nucleotide sequences in the GenBank confirmed the presence of Bean common mosaic virus strain Blackeye (BCMV-BlC) and CMV in the symptomatic beans. Sequences of BCMV and CMV isolates from the beans showed maximum nucleotide sequence identities (92-97%) and (90%), respectively with BCMV-BIC and CMV isolates from Taiwan. Each virus isolate also clustered closely with corresponding isolates from Taiwan in a phylogenetic analyses. These results provide first evidence of the occurrence of multiple infection of BCMV-BIC and CMV in the yard long been from Bogor, West Java. Key words: yard long bean, BCMV-BIC, CMV, Bogor Indonesia

Utilization of Root-Colonizing Bacteria to Protect Hot-Pepper Against Tobacco Mosaic Tobamovirus TRI ASMIRA DAMAYANTI; HENDRA PARDEDE; NISA RACHMANIA MUBARIK
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Tobacco Mosaic Tobamovirus (TMV) is one of many important viruses infecting Solanaceous plants including hot pepper in Indonesia. To accomplish and improve the effectiveness of virus management, we used root-colonizing bacteria (rhizobacteria) isolated from healthy hot pepper. Eight rhizobacteria isolates were selected and their capacity in enhancing plant growth and inducing systemic resistance (ISR) against TMV in greenhouse trials were evaluated. The rhizobacteria was applied as seed treatment and soil drench. Bacterized-seedling showed a better growth vigor, fitness and a milder symptom than non-bacterized control plants. The protective effect of rhizobacteria was more pronounced after challenging inoculation by TMV, especially for plants treated by isolates I-6, I-16, and I-35. However, TMV accumulation was slightly affected by bacterial treatment. The rhizobacteria might improved ISR by increasing peroxidase enzyme activity but this depends on the species. Based on whole results, isolate I-35 was the potential plant growth promotion rhizobacteria (PGPR). The I-35 was identified as Bacillus cereus based on morphological characteristics and nucleotide sequences of 16S r-RNA. Key words: root-colonizing bacteria, TMV, ISR

Identification and Molecular Characterization of Odontoglosum Ringspot Virus (ORSV) from Bogor, Indonesia IRWAN LAKANI; GEDE SUASTIKA; NURHAJATI MATTJIK; TRI ASMIRA DAMAYANTI
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Recently, field surveys were conducted in several orchid nurseries in Bogor (West Java), Magelang (Central Java) and Malang (East Java). We found that most of the commercially orchids sampled from Bogor was infected by virus-like disease. The symptoms depend on the orchid species. Thus, the symptoms varied such as mottle, mosaic and necrotic flecks either on the leaves or on the flowers. Similiar symptoms were not found in samples obtained from Central Java and East Java. A Tobamo-like virus was inferred to be possible cause of the viral disease-like symptoms. Serological test of the samples by ELISA showed positive against Odontoglosum Ringspot Virus (ORSV) antibody and was negative against Cymbidium Mosaic Virus (CyMV) antibody. Total RNA was extracted from symptomatic plants and RT-PCR was carried out by using a pair of coat protein (CP) gene primer of ORSV. It was successfully amplified a 500 bp of DNA fragment and it was directly sequenced. The nucleotide sequence of CP gene had confirmed the identity of ORSV. Phylogenetic analysis based on the CP nucleotide sequences were grouped into only one major cluster. The ORSV isolate from Bogor (Sd 21) and the other isolates were clustered in the same group and had highest nucleotide homology (99%). These results provide first evidence of ORSV infecting orchids in Bogor, Indonesia.

Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli Hamdayanty Hamdayanty; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available. The research was conducted to produce antigen of SCSMV coat protein (SCSMV-CP) through overexpressing it on bacterial expression which will be used for antiserum production. SCSMV-CP was amplified using specific primers for CP gene containing BamHI and HindIII restriction enzyme sites and cloned into pTZ57R/T. Subsequently, the SCSMV-CP was subcloned into pET28a and transformed on Escherichia coli BL21(DE3) and Rosetta-gami(DE3)pLysS. The concentration of isopropyl β-d-thiogalactopyranoside (IPTG), incubation temperature, and bacterial harvesting time after IPTG induction were optimized. SCSMV-CP gene was successfully amplified with size ±855 bp, subcloned into vector expression, and expressed in insoluble fraction either in both bacterial host. Optimal protein expression of SCSMV-CP recombinant was obtained at 25°C with IPTG concentration 0.25–1.00 mM and harvested at 9–12 hours after IPTG induction in E. coliBL21(DE3), and at 30°C with IPTG concentration 0.25–1.00 mM and harvested 3–12 hours after IPTG induction in E. coli Rosetta(DE3)pLysS. SDS-PAGE analysis showed that protein size of SCSMV-CP recombinant was ±35.4 kDa.

Penyebaran Virus Utama Tebu di Lampung dan Sulawesi Selatan Dwi Subekti; Sri Hendrastuti Hidayat; Tri Asmira Damayanti; Purwono Purwono
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

One effort to increase domestic sugar production is through the extension of sugarcane growing area outside Java island. The status of sugarcane disease outside Java, particularly those caused by virus(es), has not been evaluated yet; where virus diseases are able to decrease sugarcane productivity. Therefore, the aim of this research was to identify the sugarcane major viruses and their distributions in Lampung and South Sulawesi. Field survey and leaf sampling was collected from Lampung Tengah District, in Lampung Province and three districts in South Sulawesi Province i.e., Bone, Gowa, and Takalar. Virus detection was performed by reverse transcription-polymerase chain reaction (RT-PCR) method using specific primers for Sugarcane mosaic virus (SCMV), Sugarcane streak mosaic virus (SCSMV), and Sugarcane yellow leaf virus (SCYLV). Results indicated that symptoms variation was observed in the field. The highest disease incidence and severity was found in Lampung, i.e. 100% and 61.67-71.67%, respectively; whereas the lowest disease incidence and severity was found in Takalar, i.e. 5-70% and 5-45%, respectively. The disease incidence and severity of viral diseases tend to be low in dry and low rain fall conditions. This information might be helpful for the extensification program of sugarcane development in supporting the sugar self-sufficiency program. Keywords: disease incidence, disease severity, reverse transcription-polymerase chain reaction, sugar self-sufficiency

Taksasi Kehilangan Hasil oleh Penyakit Kerdil pada Kentang di Jawa Tengah Sofranita Syifa Fitriyati; Kikin Hamzah Mutaqin; Tri Asmira Damayanti
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Potato is one of economically important crops in Indonesia. However, its production fluctuates since there are some factors affecting the yield. Recently, we found dwarf disease at potato central production area in Wonosobo and Banjarnegara Regencies in Central Java. So far, there is no information related to the yield loss caused by the dwarf disease on potato. Therefore, the research aimed to estimate the yield loss caused by the dwarf disease on potato in Central Java. The yield loss estimation was determined on some potato cultivations with different disease severities. The disease intensity was observed for five times with interval two weeks on six plots. The quantity and quality of potato yields, and selling price was calculated at the end of the observation and the correlation between the severity of disease and AUDPC (Area under disease progress curve) with yield loss was determined by regression and correlation analysis. The dwarf disease reduced plant height, individual and total tuber weight without affecting the number of tuber yield. The different of disease intensities in some area affected to the percentage of yield loss. The yield loss caused by dwarf diseases on individual potato plants up to 64.42%. Yield loss estimation caused by the dwarf disease in fields with different level of disease intensities ranged from 10.35-26.43%. Regression analysis between disease severities and yield losses showed the R square value at the level of 88.6% and a correlation at the level of 94.14%. These results indicated that the correlation between the severity of dwarf disease and yield loss is high. Keywords: disease severity, potato yield, selling price, tuber quality

Co-Authors . Giyanto . Haryanto Abdjad Asih Nawangsih Ade Syahputra Akhiruddin Akhiruddin Anastasya Hondo Anastasya Hondo Annisa Nur Imamah Cahyati, Iwe Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dita Megasari Dwi Subekti Efendi, Darda Efi Toding Tondok Endah Muliarti Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Farida, Naimatul Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusti Ngurah Alit Susanta Wirya Haerani Haerani Hagia Sophia Khairani Hamdayanty Hamdayanty HENDRA PARDEDE I Dewa Nyoman Nyana Ida Parida Imamah, Annisa Nur Imaniasita, Vidya Irwan Lakani IRWAN LAKANI Islami, Nisa Fadhila Januarsih, Vera Jati Adiputra Kartika Catur Damaiyanti Ketut Ayu Yuliadhi Kikin H Mutaqin L Pebriyeni Ladja, Fausiah T. Laras Anjarsari Lilik Koesmihartono Putra Listihani, Listihani Lulu Kurnianingsih Martha Theresia Panjaitan Melinda . Mhd Rifqi Abdillah Miftakhurohmah . Miftakhurohmah Miftakhurohmah MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Mimi Sutrawati Muhammad Fikri Hafizh Munif Ghulamahdi N Mattjik Ni Putu Pandawani Niken Nur Kasim, Niken Nur Niky Elfa Amanatillah NISA RACHMANIA MUBARIK Nur Unsyah Laili Nurfadillah NURHAJATI MATTJIK OLUFEMI JOSEPH ALABI Pratiwi, Rizky Nomi Purnamawati, Iis Purwono Purwono Putri, Maharani Mustika R Kartika RAUF, AUNU RAYAPATI ADIKESAVULU NAIDU Rita Noveriza Rita Noveriza Rizki Haerunisa S Susanto Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sugeng Santoso Sugeng Santoso Supramana Suryo Wiyono Syaiful Khoiri TRISNA AGUNG PHABIOLA Wahyudin, Denih Wieke Mei Dina Yunita Fauziah Rahim Yunita Fauziah Rahim Yusmani Prayogo Zulfi, Fahma Zakiya

Title

Found 66 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 66 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 66 Documents
Search