Jurnal Sains dan Teknologi Laboratorium Medik
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
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ANALISIS TELUR CACING SOIL TRANSMITTED HELMINTH (STH) PADA PETANI SAYUR DI KUBANG RAYA KOTA PEKANBARU
Berliana N.R.S. Aritonang
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.99
Worm infection caused by the Soil Transmitted Helminth (STH) are still a health problem for the community, especially for vegetable farmers whose work is directly related to land. Worm whose development requires soil to be an infective form, namely the Soil Transmitted Helminth (STH). The types of Soil Transmitted Helminth (STH) worm are Ascaris lumbricoides, Trichuris trichiura, Ancylostoma duodenale and Necator americanus. The purpose of this study was to identify the STH eggs in stools of vegetable farmers at Kubang Raya Pekanbaru. The sampling technique used was quota sampling. The examination method used were with mount direct and sedimentation method. Based on microscopic examination of 6 faecal samples with the direct method, STH eggs were not found in all sample. Meanwhile in the observation with sedimentation method, 1 of 6 samples was found to be positive for hookworm eggs (sample GP).
PERBANDINGAN PENGGUNAAN METODE CARIK CELUP DAN METODE ASAM ASETAT 6% DALAM PEMERIKSAAN PROTEIN URIN
Hartini H
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.101
Proteinuria merupakan keadaan terdapatnya protein didalam urin (>150 mg) dalam 24 jam. Proteinuria mengindikasikan adanya kegagalan fungsi ginjal karena glomerulus dan tubulus distal ginjal tidak berfungsi dalam menyaring urine. Pemeriksaan protein urine konvensional secara umum dapat dilakukan dengan 2 metode yaitu metode carik celup dan asam asetat 6%. Berdasarkan hal tersebut maka dilakukan penelitian untuk membandingkan hasil pemeriksaan protein urine metode carik celup dan metode asam asetat 6%. Penelitian ini merupakan jenis penelitian non eksperimental dengan desain cross sectional dan teknik sampling menggunakan accidental sampling. Sampel yang digunakan adalah sampel urin yang berasal dari pasien proteinuria di Rumah sakit X Pekanbaru. Hasil penelitian yang dianalisis dengan uji t-tidak berpasangan diperoleh p > 0,05 artinya tidak ada perbedaan bermakna antara protein urine yang diperiksa dengan metode carik celup dan metode asam asetat 6%. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemeriksaan protein urine menggunakan metode carik celup dan asam asetat 6% dapat dilakukan di laboratorium untuk menegakkan hasil pemeriksaan.
LITERATURE REVIEW : PERBANDINGAN EFEKTIVITAS PEMERIKSAAN KULTUR DAN POLYMERASE CHAIN REACTION (PCR) DALAM IDENTIFIKASI Neisseria gonorrhoeae PADA PASIEN GONORE
Sahira Rauf
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.102
Gonorrhea is a disease caused by the bacterium Neisseria gonorrhoea that generally attacks the genital mucosa or cervical mucosa. Gonorrhea has a higher incidence than other infectious diseases. The spread of this disease is higher in developing countries including Indonesia. Culture examination is the gold standard for the diagnosis of gonorrhea, this examination has good sensitivity and very high specificity, while the weakness is that it takes a long time to get positive results. Polymerase Chain Reaction (PCR) is an in vitro technique that can amplify a specific DNA section that lies between two known DNA sections. PCR is a biomolecular technique used for the isolation and exponential amplification of target DNA fragments or sequences through enzymatic replication, or without the use of living organisms. The PCR technique is faster than culture, effective, accurate, efficient, high sensitivity and specificity. This study aims to determine the effectiveness and differences in the results of culture and Polymerase Chain Reaction (PCR) in identifying Neisseria gonorrhoea in gonorrhea patients. This study used a literature review conducted by collecting library data based on PICO keywords using Google Scholar and PubMed databases. The results of the statistical test for sensitivity were 0.001 (p<0.05) and statistical specificity test was 0.095 (p>0.05). There was a significant difference in terms of sensitivity and there was no significant difference in terms of the specificity of the results of culture and Polymerase Chain Reaction (PCR) examinations.
THE OVERVIEW OF HEMOGLOBIN LEVELS WITH CYANMETHEMOGLOBIN METHOD AND VOLUMETRIC IMPEDANCE IN PATIENTS EXPOSED TO MOSQUITO REPELLENT SPRAY
Andy Setiawan Widodo
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.107
This study aims to determine differences in the results of hemoglobin levels of people exposed to mosquito repellent spray examined using Cyanmethemoglobin and Volumetric Impedance methods. This study employed a cross sectional design. The sample in this study amounted to 30 patients at Pandak 2 Primary Health Center Bantul. The data used secondary data obtained at Pandak 2 Primary Health Center Laboratory, Bantul. The data processing stages carried out in this study were editing, coding, and tabulating which would then the data was analysed using Independent sample T test. The Cyanmethemoglobin method showed that there were 11 patients with normal hemoglobin levels and the remaining 19 patients showed hemoglobin levels below normal, while the Volumetric Impedance method revealed 13 patients with normal hemoglobin levels and the remaining 17 patients showed hemoglobin levels below normal. Based on the results of the T sample Independent Test, there was no significant difference between the Cyanmethemoglobin and Volumetric Impedance methods in the examination of hemoglobin levels in patients exposed to mosquito repellent spray.
PENGARUH KONDISI PANDEMI TERHADAP KUALITAS UDARA AMBIEN (SO2 dan NO2) PADA TITIK KEMACETAN KOTA YOGYAKARTA
Nur Hanifa Fajar Wulandari
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.108
Penelitian ini bertujuan untuk menganalisis perbandingan kualitas sulfur dioksida dan nitrogen dioksida pada kondisi sebelum terjadi pandemi, saat terjadi pandemi, dan pasca pandemi pada tahun 2019 hingga 2021. Lokasi penelitian di perempatan jalan raya Kota Yogyakarta. Pemeriksaan ini menggunakan metode pararosanilin dan Griess-Saltzman. Penelitian ini menggunakan rancangan deskriptif kuantitatif. Sampel pada penelitian ini berjumlah tujuh lokasi yang merupakan wilayah sampling Dinas Lingkungan Hidup Kota Yogyakarta. Data yang digunakan dalam penelitian ini menggunakan data sekunder yang didapatkan di Laboratorium Dinas Lingkungan Hidup Kota Yogyakarta. Tahap pengolahan data pada penelitian ini yaitu melakukan pengecekan data untuk memastikan data yang di perlukan sudah tersedia kemudian dilakukan analisis data menggunakan Uji One Way Anova. Hasil penelitian ini menunjukan konsentrasi sulfur dioksida masih di bawah batasan pencemaran udara. Hasil pengukuran nitrogen dioksida masih di bawah batasan pencemaran udara. Berdasarkan kategori ISPU pengukuran sulfur dioksida dan nitrogen dioksida menunjukan hasil dalam kategori baik. Berdasarkan hasil Uji One Way Anova terdapat perbedaan pengaruh di setiap tahunnya pada konsentrasi sulfur dioksida dan nitrogen dioksida.
IDENTIFIKASI Candida sp DALAM URIN IBU HAMIL DI KLINIK RAMLAH PARJIB 1 SAMARINDA
Annisa Rahmawati
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.110
Based on the survey results, there are several pregnant women who experience symptoms of candidiasis vulvovaginal. All pregnant women who visited the Clinic Ramlah Parjib 1 Samarinda on 15 February 2022, admitted that they had never carried out laboratory tests such as examination of urine and vaginal secretions. This study aims to identify the Candida sp fungus in the urine of pregnant women at Clinic Ramlah Parjib 1 Samarinda. This type of research is descriptive and uses a consecutive sampling technique with a sample of 30 pregnant womens urine. Respondents in this study were pregnant women. Samples were obtained from the urine of pregnant women at Clinic Ramlah Parjib 1 Samarinda which met the inclusion and exclusion criteria, then microscopic tests. The results showed that 8 people had Candidiasis vulvovaginal , 26,7% were positive Candida sp and 22 people, 73,3% were negative Candida sp. In addition, the growth of contaminant but harmless fungi was found, namely Aspergillus sp as many as 6 samples with a percentage of 20%.
IDENTIFIKASI TELUR SOIL TRANSMITTED HELMINTHS (STH) PADA FESES ANAK-ANAK DENGAN METODE DIRECT SLIDE DAN PENGENDAPAN FORMALIN 10%
Yeli Hartuti
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.111
Worm infection is one of the diseases that is still a health problem in children in developing countries, especially in children who have not been able to maintain personal hygiene.Worm infections can be caused by soil-transmitted worms that have been contaminated by the eggs or larvae of the Soil Transmitted Helminth worm. Identification is done by 2 methods, namely Direct slide method and 10% formalin deposition. This research is a type of non-experimental research with cross sectional design and sampling technique using purposive sampling. The sample used is the feces of children aged 5-11 years. The results of the study were 17 samples of feces that were examined, it was found that there were 6 samples infected with STH (Positive) while the other 11 samples were not infected with STH (Negative). Frequency Distribution of Mother's Knowledge about Worm Infections, good knowledge of 11.76%, 29.41% sufficient and 58.82% less.
ANALISIS KADAR VITAMIN C PADA BAWANG MERAH (Allium cepa L.) JENIS BATU, SUMENEP, DAN TUTUG DENGAN METODE SPEKTROFOTOMETRI UV-VIS
Dadang Muhammad Hasyim
Jurnal Sains dan Teknologi Laboratorium Medik Vol 8 No 2 (2022): November (2022)
Publisher : Akademi Kesehatan John Paul II Pekanbaru
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DOI: 10.52071/jstlm.v8i2.121
Shallot (Allium cepa L.) is a vegetable spice that is widely used as a food seasoning to add flavor and enjoyment to cooking. Shallots are also widely used as a powerful traditional medicinal ingredient to treat various health problems. Shallots contain various kinds of nutrients and vitamins. One of the vitamins contained in shallots is vitamin C which acts as an antioxidant and is effective in dealing with free radicals that damage cells or tissues. This study aims to determine the levels of vitamin C in several types of shallots. The types of shallots in this study were stone shallots, sumenep shallots, and tutug shallots. Analysis of vitamin C levels was carried out using the UV-Vis spectrophotometry method at a wavelength of 515 nm. Results of the analysis showed that the vitamin C levels of stone shallots were 1.043 mg/100 g, Sumenep shallots were 1.638 mg/100 g, and tutug shallots were 1.296 mg/100 g. Vitamin C levels in Sumenep shallots have the highest levels compared to those of stone shallots and tutug shallots.