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Journal La Lifesci
Published by Newinera Publisher
ISSN : 27211304     EISSN : 27211207     DOI : https://doi.org/10.37899/journallalifesci
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology
International Journal La Lifesci is peer reviewed, open access Academic and Research Journal which publishes Original Research Articles and Review Article editorial comments etc in all fields of life sciences including Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology, Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 170 Documents
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Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water Ervandi, M.
Journal La Lifesci Vol. 4 No. 6 (2023): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v4i6.1191

Abstract

This study aims to determine the role of the best diluent in protecting the integrity of the spermatozoa membrane after storage using an alternative diluent formulation of red fruit extract (Pandanus Conoideus Lamk) and coconut water. An alternative diluent formulation featuring red fruit extract (Pandanus Conoideus Lamk) and coconut water was utilized. Fresh semen from Bali Bull was evaluated in the Integrated Field Laboratory, Department of Animal Science of Universitas Muhammadiyah Gorontalo. Bali bull sperm of high quality was diluted using a combination diluent containing red fruit extract (Pandanus Conoideus Lamk), green coconut water, and CEP-2 mixed with 10% egg yolk. Technical term abbreviations were explained at their initial use. Spermatozoa membrane integrity was assessed using the hypoosmotic swelling test (HOST), while chlortetracycline (CTC) fluorescent dye was used to evaluate spermatozoa capacitation and acrosome reaction. The randomized group design (RAK) was used in the research, consisting of four conditions and 10 replications. P0 served as the control group, which received CEP-2 plus 10% egg yolk. P1 was administered EBM diluent AK enriched with 15% KT, while P2 received EBM diluent AK plus 15% KT and fructose 1 mg/ml. P3 was given EBM diluent AK plus 15% KT and fructose 2 mg/ml. Analysis of variance was used to examine the resulting data. The findings indicated that CEP-2 plus 10% egg yolk diluent was more effective than the diluent formulations containing red fruit extract and coconut water. Spermatozoa with intact membranes and no capacitation remain high, while those with capacitation and acrosome reaction remain low.
Assess the Opinions of Nurses in Hospitals in the Al-Diwaniah Governorate on the Most Common Drug Errors and the Factors that Lead to Them Kareem, Haidar Jabbar; Fareed, Wasen Abdul‑Ameer Ali; Hamed, Dhyaa Shinyar
Journal La Lifesci Vol. 5 No. 3 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i3.1246

Abstract

This study aims to investigate nurses' perspectives on medication errors and the factors that lead to them. A total of 355 nurses from three general hospitals and a private facility in Aldiwaniah City participated in this descriptive cross-sectional research. We created a five-part self-administered questionnaire based on previously published research and gave it to the nurses throughout their shifts. We contacted 355 nurses, and 352 of them completed the questionnaire. The data analysis employed descriptive statistics. According to the study's results, giving medication at the wrong time was the most common type of medication mistake, scoring 2.5±1.02. Errors with a lower severity were less common. 64% of nurses did not report prescription mistakes, but 44.6 percent did so orally, and 56.8% did so in the form of an event report. The most frequent obstacle to reporting was a misunderstanding of what defines a drug error, indicating a substantial discrepancy between their perception and knowledge. Common medication errors (MEs) that nurses describe include administering medication to the incorrect patient at the wrong time, delivering IV medication at the incorrect speed, and forgetting to add a dosage. Both environmental factors (frequent visits) and personal factors (stress and fatigue) contribute to these errors. There is a statistically significant correlation between the incorrect time and characteristics such as ward, hospital type, age, gender, and educational attainment. There is a strong correlation between systemic problems and incorrect time errors.
Study of Some Immunologic Parameters in Aborted Women Infected with Toxoplasma Gondii Husham, Hiba Aeiad; Al-Mussawi , Kawther A. M.
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1247

Abstract

The demographic data of the patients was collected using a pre-designed questionnaire that included information on age and exposure to animals. Written permission was obtained from all participants in this research.The research was conducted in Karbala Maternity and Pediatric Hospital as well as other private labs in Kerbala city, Iraq, from November 2022 to May 2023. The blood samples underwent centrifugation, and the levels of serum IgM, IgG, interleukin-27 (IL-27), and Monocyte Chemoattractant Protein-1 (MCP-1) were measured using the enzyme-linked immunosorbent assay (ELISA) technique. The findings indicated that a total of 53 female individuals had abortion as a direct consequence of toxoplasmosis infection. The age group with the greatest incidence rate (56.6%) of toxoplasmosis was 18-26 years, whereas 79.2% of the patients were found to have had contact with animals. The blood immunoglobulin level analysis revealed that 98.1% of patients exhibited positive IgG findings, while 1.9% tested positive for IgM. In contrast, all healthy women in the control group tested negative for both IgM and IgG. The study found that women infected with toxoplasmosis had significantly higher levels of serum IL-27 (645.95 pg/ml) compared to the control group (88.26 pg/ml). Similarly, the infected women had significantly higher levels of MCP-1 (272.20 pg/ml) compared to the control group (48.83 pg/ml). These differences in interleukin levels were highly significant (P<0.0001). The present investigation indicates that women with recurrent abortion who were infected with T. gondii had elevated levels of IgG, IL-27, and MCP-1.
Article Review: Skin Diseases in Dromedary Camels Gharban, Hasanain A.J.
Journal La Lifesci Vol. 5 No. 3 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i3.1256

Abstract

The dromedary camel (Camelus dromedarius) is a significant livestock species in several nations located in desert and semi-arid regions of Asia, Northern and Eastern Africa, South America, and the high highlands of the Andes. In Iraq, the FAO figures of 2014 indicate that there are around 65,000 camels, all of which belong to the species Camelus dromedarius and have a single hump. Camels are the primary source of sustenance for all Bedouin clans and villages in various ecological zones in Iraq. This dependence involves the usage of camel milk, meat, leather, and wool. Furthermore, camels have been used as beasts of burden, means of transportation, and mounts. Camels, typically grown in their native desert environment, experience significant stress due to the prolonged dry season. This stress makes them vulnerable to many illnesses and health issues. Historically, owing to little research on camel ailments, doctors believed that camels were immune to many disease-causing causes. Scientific evidence has shown that camels, like other livestock or maybe even more so, are vulnerable to the same disease-causing viruses that impact other animal species. Camels often suffer from illnesses that are challenging to manage because to their non-specific symptoms and similarity to diseases seen in other ruminant animals. Additionally, camels have some resistance to some infections. This research attempts to provide a concise description of the most frequently seen skin disorders in dromedary camels in Iraq.
Serological Detection of Toxoplasma Gondii in Animals and Human in Baghdad Province, Iraq Shatti, Ali A.
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1262

Abstract

This study aims to investigate the prevalence of Toxoplasma gondii in field animals (cattle and sheep) and their owners at various areas in Baghdad province (Iraq) during September (2023) and February (2024). Also, it aims to identify the role of cat in transmission of infection to humans and animals. A total of 222 venous blood samples including 84 humans and 138 animals were collected under aseptic conditions into free-anticoagulant glass tubes. In addition, a total of 112 fecal cat samples were collected from different areas in Baghdad city into plastic containers to be examined by the flotation method. The results showed that 84 blood samples from animal owners detected the infection rate were 36.90%. In addition, there is a relationship between infection and age. The study shows that the highest age for infection is 26-30 years, and the percentage was 66.66%. also, the study included examining 138 blood samples from animals divided into 68 sheep and 70 cows’ infection parasite rate was also 41.30%, and through examining the collection of 120 samples of soil from different areas in the city of Baghdad, the percentage of infection with T. gondii parasite reached 32.50%, addition to the infection rate in cats was 50% by examining 112 stool samples collected from different areas in the same city. This study reported the increasing seroprevalence of T. gondii using different samples. The reason for this increase may be due to poor health conditions, the large number of stray cats, the accumulation of dirt, the low degree of health education, and ignorance of the sources of infection for the disease and the attempt to avoid them, as it was noted through the study that a large number of women raise cats in their homes. They do not know that cats are the source of the disease.
Role of Plant Density on Vegetative Growth and Yield of Four Faba Bean Cultivars Al-Jubouri, Amer Abdullah Hussein
Journal La Lifesci Vol. 5 No. 3 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i3.1263

Abstract

The study was conducted in the vegetable field of the Department of Horticulture and Landscape Engineering, College of Agriculture and Forestry, University of Mosul, during the agricultural season 2018-2019 to study the effect of plant density on the vegetative growth characteristics and yield of four bean varieties, that is, by two factors: plant density (8 plants / m2 and 12 plants / m2) and cultivars. (Local, Spanish, French, Dutch) within the (RCBD) design with three replications, as the number of transactions in the experiment was (8) . The plant density of 8 plants/m2 showed its superiority in most of the characteristics of vegetative growth and yield, except for the two characteristics of plant height and pod weight, where it was superior under the plant density of 12 plants/m2. The local cultivar excelled in most of the characteristics of vegetative growth and the characteristics of total yield and plant yield compared with the Dutch or French variety. Give the lowest values for these traits.
Implementation of Millennial Farmer Development Policy in Bogor Regency Casroni, Muhamad; Suryana, Asep Agus Handaka; Rusliandy, Rusliandy
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1297

Abstract

The development of millennial farmers has a very important role in preparing for the regeneration of farmers in the future. However, the Bogor district government has a number of weaknesses and obstacles in fostering millennial farmers. The research analyzes the implementation of millennial farmer policies in Bogor Regency from the aspects of policy context and policy content according to Grindel. The method was used with a qualitative approach, with data collection techniques using observation and interviews and data analysis using triangulation techniques. The results showed that the implementation of the millennial farmer development policy in Bogor Regency had gone well but is faced with several weaknesses and obstacles, namely socialization for agricultural extension workers is still lacking, limited budget, limited facilities and infrastructure, cross-sectoral collaboration has not been optimal and limited agricultural land ownership. The research recommendations are to increase the socialization of millennial farmer development policies, increase budgets, provide support for facilities and infrastructure, optimize cross-sector roles, and provide support and facilitation for agricultural land.
Investigation of the Resistance of Klebsiella Bacteria to Antibiotics and the Synthesis of Virulence Factors Isolated from Various Pathogenic Infections Ali, Farah
Journal La Lifesci Vol. 5 No. 4 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i4.1245

Abstract

The purpose of the study is to establish the level of antibiotic resistance in Klebsiella pneumoniae and to evaluate the production of virulence factors of the isolates originating from patients with diverse infections in Baghdad. In this study,75 clinical samples, including 32 samples of wounds, fifteen burn samples, 17 sputum samples and eleven samples of urine were collected from patients afflicted with different infections from some hospitals in Baghdad city, during the period from October to March 2023. It was found that 22 of the isolates were of Klebsiella pneumonia isolates after culturing the clinical samples on MacConkey agar, blood agar, and Eosin methylene blue medium, in addition to studying the morphologic appearance, biochemical tests, and confirmation of diagnosis using the VITEKA2 system. The antibiotic sensitivity test showed that all isolates showed (100%) multi-drug resistance to 7 antibiotics including Cefotaxime, Ampicillin, Chloramphenicol, Gentamicin, Rifampicin, while most of the isolates were sensitive to Azithromycin. The investigation of certain virulence factors of the Klebsiella pneumonia bacteria revealed that all of the isolates were encased in a capsule and were incapable of producing the hemolysin enzyme, but they were all able to produce the urease enzyme and form biofilm. The isolates produced bacteriocin at a rate of 12%.
Role of NPK Fertilizer and Planting Ates on the Vegetative and Flowering Growth Tributes of Two Varieties of Snake Melon Al-Sharabi, Khaled Mustafa Ali; Al-Jubouri, Amer Abdullah Hussein
Journal La Lifesci Vol. 5 No. 4 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i4.1267

Abstract

The purpose of this research was to determine the response of two varieties of snake melon to the levels of NPK fertilizer and planting dates in terms of vegetative and flowering growth characters. The experiment was conducted in the vegetable field of the Department of Horticulture and Landscape Engineering/College of Agriculture and Forestry University of Mosul during the spring season 2023. The study included three factors. The first factor: Two local cucumber varieties: (gennxt and Mosli). The second factor was compound fertilizer (NPK) at four levels (270, 180, 90.0 kg/ha!) and the third factor: planting dates. Two planting dates were chosen: (4/1/2023) and (4/20/2023). Compound fertilizer (NPK) was added to the soil in three stages, the first three weeks after planting and after the plants reached the appropriate size (four true leaves), and the second and third at an interval of 20 days between one stage and the next. Thus, the experiment included 18 treatments (2 x 2 x 4 = 16). With three replications, the experiment was carried out in the field using a factorial experiment according to a randomized complete block design (RCBD), where the treatments were randomly distributed according to the design used in the experiment. The results were analyzed statistically according to the design used, and the means were compared according to Duncan's multinomial test at the probability level of 0.05.
Traditional Detection of Intestinal Helminths in Stray Cats in Wasit Province, Iraq Aqeele, Ghasik
Journal La Lifesci Vol. 5 No. 4 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i4.1286

Abstract

The purpose of this study was to find out the prevalence and distribution of intestinal parasites namely helminths in stray cats in different areas of Wasit Province, Iraq with reference to zoonotic risks for improved public health intervention. The study included a survey of helminthes in the intestines of stray cats in several areas of Wasit province, to determine the extent of their spread among populations in the regions. Samples were collected from 96 stray cats distributed from several areas of the same province, 7 species of parasitic were observed, in the intestines and four species tapeworms. The highest observed was of Dipylidium caninum for 44.61% of cats stray infected with tapeworms. The other two species recorded nematodes as 46.51%, and 22.58% for Toxocara cains, and Toxascaris leonina respectively, note that T. cains infection is higher than the others; while, other species have been diagnosed with different rates 35.38%, 24.61%, 12.30%, and 27.90% for Taenia multiceps, Taenia ovis, Taenia pisiformis, and Ancylostoma sp., respectively. The infection rate in the northern regions of the province was higher in cats than in the southern regions of Wasit Province with nematodes and tapeworms. The extensive presence of stray cats across numerous Iraqi areas necessitates increased focus from relevant bodies, especially in public health and veterinary sectors, to mitigate associated risks and zoonotic concerns. Consequently, necessary to enhance the scope of studies and investigations concerning cats and their ailments, attributed to the proliferation of feline reproduction within communities and their interaction with stray felines.

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