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INDONESIA
Journal La Lifesci
Published by Newinera Publisher
ISSN : 27211304     EISSN : 27211207     DOI : https://doi.org/10.37899/journallalifesci
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology
International Journal La Lifesci is peer reviewed, open access Academic and Research Journal which publishes Original Research Articles and Review Article editorial comments etc in all fields of life sciences including Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology, Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 170 Documents
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Coaching Studies Morals in Conservation Efforts Environment Sustainable Living (Case Study in Schools River Ciliwung Srengseng Sawah Jakarta) Faisal, Juwito; Sauri, Sofyan; Tanjung, Hendri; Ibdalsyah, Ibdalsyah
Journal La Lifesci Vol. 4 No. 6 (2023): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v4i6.992

Abstract

This research is research that uses a qualitative research paradigm using descriptive qualitative methods, where data collection is carried out through observation, interviews and documentation. The purpose of this study is to know the steps taken in setting goals, planning, implementing and evaluating moral development on the environment carried out at the Ciliwung River School Srengseng Rice Fields, Jakarta. This research method is with in-depth interviews, documentation, and observation. Data analysis techniques with correlation techniques and data validity techniques with source and method triangulation techniques. The results of this study concluded that the form of implementing moral education values towards the environment to students is carried out through learning and non-learning has been running very well. The role of school coaches and teachers as mentors for activities in implementing the values of moral education in the environment for students of Ciliwung River School Srengseng Sawah Jakarta and the surrounding community have been going well. The implementation process is through the four roles of school coaches, scholars, and teachers as activity mentors as educators, motivators, and facilitators, and evaluators in the learning process and daily activities in the classroom and outside the classroom.
Learning from Community Practices: Social Capital of Farming Communities in Supporting Sustainable Agriculture Widjayanthi, Lenny; Kusmiati, Ati; Ibanah, Indah; Agung, Sih; Wijayanto, Yagus; Wulanjani, Distiana; Prastowo, Sigit; Gabrillo, Christina A.
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1017

Abstract

The use of chemical production facilities in farming, both food crops and horticultural crops, has an impact on agricultural land and human health, as well as causes damage to the environment, soil, water, air and living creatures. To achieve success in sustainable development, this is done by identifying social capital and developing farmer capacity. The research objectives are to identify sustainable agricultural practices carried out by farmers, identify community social capital in supporting sustainable agriculture. The research area was determined purposively in Pontang Village, Jember district. Determination of the research sample was carried out by simple random sampling on rice farmers. The research sample consisted of 106 respondents (margin of error of 10%). The data used in this research are primary and secondary data obtained from interviews, observations and document studies. Data analysis was carried out descriptively using scoring and categorization. Some sustainable agricultural practices carried out by communities in the research area are: organic agriculture, crop rotation and polyculture, soil management and agroforestry. Extension workers also conduct Field Schools (FS) for farmers. Farmers are given the knowledge and skills to utilize agricultural waste around them to make organic fertilizer. Extension workers also teach farmers in making PGPR, Beauveria bassiana, Pseudomonas fluorescens (Pf), Trichoderma, and planting refugia in rice fields. The social capital of the farming community in supporting sustainable agriculture is in the moderate category, as well as the supporting dimensions such as the social, norm and network dimension are also in the moderate category.
Groundwater Potential of the East and North Sides of Gunungmasigit Village: A Case Study Wijaya, Dwi Suyandi Pasa; Iskandarsyah, Teuku Yan W. M.; Yoseph, Boy
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1039

Abstract

The purpose of this research is to conduct a groundwater survei by analyzing the availability of groundwater on the east and north sides of Gunungmasigit Village, Kec. cipatat, kab. west bandung (case study: PPSDM Geominerba field campus KESDM). The aim of this study was to determine the potential for groundwater in the northern area of the PPSDM Geominerba Field Campus and to find information regarding the absence of groundwater after drilling was carried out in 2019 in the area on the east side of the PPSDM Geominerba Field Campus. The research administrative area is located in Gunungmasigit Village, Kec. Cipatat, Kab. West Bandung (PPSDM Geominerba field campus KESDM). The author will focus more on the end of Cipatat-1 (north side) on the map, where it is located in the gate area of the Geominerba PPSDM Field Campus. This study uses the geoelectrical measurement method. One of the geoelectrical methods is the resistivity or resistivity method. This method studies the subsurface structure based on the different resistivity values in the rock with depth. Data analysis used IPI2WIN Software with track 1 having a length of 200 meters and track 3 having a length of 400 meters. In the results of Cipatat-1 it is suspected that there is a water-carrying layer at a depth of 11.9 – 45.9 m. Meanwhile, the results of Cipatat-3 suggest that there is a water-carrying layer at a depth of 26.3 m.
Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi Santoso, Tri Joko; Husni, Ali; Nugroho, Kristianto; Ya’la, Zakirah Raihani; Dewi, Triyani; Marhawati, Marhawati; Maemunah, Maemunah; Rosyida, Eka; Ndobe, Samliok
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1062

Abstract

Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.
Assessment of Farm-Level Practices of Disease and Pest Management in the Banana Zone of Nawalparasi West district, Nepal Ghimire, Sudip; Regmi, Anmol
Journal La Lifesci Vol. 5 No. 3 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i3.1139

Abstract

Banana (Musa paradisiaca) is a vital fruit crop in Nepal, especially in the Nawalparasi West district, where it can produce and generate income. However, banana farming faces many problems such as diseases and pests that reduce crop yield and quality. This research aimed to assess the state of pest control techniques and banana diseases, providing insights and recommendations for their improvement. A total of 125 banana farmers were selected and the data were analyzed using Microsoft Excel and the Statistical Package for the Social Sciences (SPSS). The findings revealed that the banana leaf and fruit scarring beetle emerged as a particularly troublesome pest (severity index: 0.93), closely followed by the banana aphid (0.67), banana stem weevil (0.51), mealy bug (0.46), and banana rhizome stem borer (0.34). Similarly, diseases such as sigatoka leaf spot exhibited the highest severity (0.76), followed by anthracnose (0.59), bunchy top virus (0.52), bacterial wilt (0.51), and panama wilt (0.43). All participants employed diverse pest management practices, including mechanical, cultural, biological, and chemical methods. Notably, 82.4% utilized fungicides and 100% employed cultural methods, with no adoption of biological approaches. In pest management, 31.2% employed mechanical/physical methods, 95% embraced cultural methods, and all the respondents used pesticides. Positive and significant relationships were identified between respondents' education level, training, farming experience, contact with extension agents, and adoption of disease and pest management practices in banana cultivation.
DNA Profiling, Bioinformatics and Databases in Forensics: Human Identification Purposes Meilana, Andi Nur Sakina Tri; Auerkari, Elza Ibrahim
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1171

Abstract

Individual identification is an essential element in establishing truth the forensic investigation process, be it in criminal, medico-legal, or mass disasters case. When ante-mortem data are not available, the only thing that can be used is identification trough DNA. Alec Jeffrey, a geneticist (1984), found that certain regions of DNA contain repetitive sequences and the number of repetitions in individuals differs from one another. This identification method known as DNA profiling. DNA profiling is described as an important and significant discovery in forensics and has been considered as the standard in modern human identification. Until now, the recommended DNA profiling method must be based on the PCR to analyze degraded DNA and short-sized DNA (Short Tandem Repeats) through PCR amplification. In profiling, the result DNA amplification are analyzed through genetic markers (DNA Markers) and then matched into the database or compared with the collected reference samples. In the human genome, the genetic markers most frequently used in forensics are autosomal STR, Y-STR, mtDNA, X-STR, SNPs, and Amelogenin. Accomplishment of proving the suitability of DNA profiles, an application of bioinformatics in forensics is carried out. Bioinformatics is a combination of molecular biology and computer informatics that aims to manage and analyze data and store biological (genetic) information. DNA database is an application of bioinformatics in the form of special software which has become an important tool for biologists and forensics. This genetic database will predict the similarities between one DNA profile and another.
Morphological and Morphometric Analysis of Orocraniofacial Bones for Estimative Identification Gender Sari, Wulan Purnama; Soedarsono, Nurtami; Puspitawati, Ria
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1172

Abstract

Identification of sex estimation is the first step in the identification process and is one of the priority parameters in the forensic identification process. Identification is made when the condition of the body is unrecognizable due to natural disasters, transport accidents, bomb explosions, ethnic studies and crime investigations, where only a few parts of the body are already incomplete and limited information are available. In forensic odontology, morphological and morphometric analysis of the orocraniofacial bone is an important component that can help in the process of identifying sex estimation because it has different shapes and sizes in each individual and population. This article will discuss morphological and morphometric analysis of several parameters of the orocraniofacial bone, as well as the advantages and limitations of both methods.
Testing The Antimicrobial Effectiveness of Manila Sauce Leaves (Manilkara Zapota L) against Bacteria Escherichia Coli: In Vitro Study Azizah, Namirah Nurjiranah; Kartika, Irna Diyana; Hasbi, Berry Erida; Muchtar, Amrizal; Arsal, Andi St. Fahirah
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1173

Abstract

Diarrhea remains a significant public health concern globally, particularly affecting children under five years old. Traditional herbal remedies, including Manila Sapodilla (Manilkara zapota L) leaves, have been utilized in Indonesia for treating diarrhea due to their perceived efficacy and safety. This in vitro study aimed to assess the antimicrobial effectiveness of Manila Sapodilla leaf extract against Escherichia coli bacteria, a common cause of diarrhea. True experimental design was employed, utilizing various concentrations (25%, 50%, 75%, and 100%) of Manila Sapodilla leaf extract. The extract was obtained through maceration using ethanol followed by dilution with distilled water. Antimicrobial activity was evaluated using the Kirby-Bauer diffusion method, measuring inhibition zones formed around Escherichia coli colonies. Results demonstrated that Manila Sapodilla leaf extract exhibited inhibitory effects against Escherichia coli at all tested concentrations, albeit with varying degrees of effectiveness. The inhibition zones ranged from 5.37 mm to 8.53 mm, indicating resistance. Positive control using Ciprofloxacin displayed a significantly larger inhibition zone (21.75 mm), indicating sensitivity. These findings suggest the potential of Manila Sapodilla leaf extract as an alternative treatment for Escherichia coli infections. However, further research, including phytochemical analysis and exploration of different extraction methods and concentrations, is warranted to elucidate its therapeutic potential fully. This study contributes to the ongoing search for alternative herbal-based antibiotics to combat bacterial infections effectively.
Testing Alternative Hydroponic Nutrition of Wick System on Vegetable Growth Planted in Limited Land Hayati, Rita; Armadi, Yukiman; Yusnaweti, Yusnaweti; Feni, Rita; Asfaruddin, Asfaruddin; Ariyani, Farida; Kesumawati, Neti; Suzanna, Eka; Sarina, Sarina
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1177

Abstract

This research aims to obtain suitable alternative nutrients for vegetable cultivation using the Hydroponic Wick System so that the production costs of hydroponic cultivation can be cheaper and more affordable. The research was conducted in the Tebeng area, Ratu Agung Kodya Bengkulu, at an elevation of 50 meters above sea level. The research method used was a Randomized Block Design with a Factorial pattern (RAKF). The first factor had 3 levels of vegetable types: kailan (S1), caisim (S2), and pakcoy (S3). The second factor had 3 levels of nutrients: AB Mix 100% (N1), alternative 100% (N2), and ABMix 50% + alternative 50% (N3). Thus, there were 9 treatment combinations with 3 replications. The results of the research showed that the plant height, number of leaves, wet weight, and dry weight of plants using Alternative Nutrients for kailan, caisim, and pakcoy vegetables significantly influenced the use of AB Mix nutrients, Alternative Nutrients, and the mixture of both nutrients when grown using the Hydroponic Wick system. The best results were achieved using alternative nutrients. This was evident from the tallest plant height at harvest, which was 34.20 grams at 42 days after planting (HST), 16.62 leaves, 142.69 grams wet weight, and 7.60 grams dry weight. These results were significantly better compared to using other nutrients, offering a potential solution to the high costs of hydroponic cultivation due to the expensive price and limited availability of AB Mix nutrients, especially in the region.
Success Rate of Frozen Embryo Transfer through Assisted Hatching Laser Method and Tyrode Acid Chemical Method in Day 3 and 5 Embryos Hartini, Prastuti Dwi; Lisa, Lucy; Nurcahya, Harini; Irmansyah, Frizar; Gunarwati, Deka Putri
Journal La Lifesci Vol. 5 No. 2 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i2.1183

Abstract

Repeated implantation failures in IVF, aging in women and vitrification at low temperatures cause the embryonic zona pellucida to harden.Technique assisted hatching (AH) laser and chemical methods of tyrode acid can help the process hatching in embryos in improving implantation. The purpose of this study is to determine the relationship between pregnancy success through AH laser method and chemical method of tyrode acid at the embryonic stage day to-3 (D3) and day to-5 (D5) on Frozen Embryo Transfer (FET). The study used a retrospective cohort with a population of 95 patients using AH laser method and 36 patients with tyrode acid chemistry. The results showed the incidence of pregnancy in laser treatment in 27 patients (28.4%) and 14 patients (38.9%) in chemical treatment. Data analyzed by statistical tests Chi-square with a 95% confidence degree and a p< value of 0.05. Exist The relationship between the incidence of pregnancy FET D3 and FET D5 laser treatment, but in chemical treatment no significant difference was found. Logistic regression modeling tests show the chemical method FET D5 has a greater chance of pregnancy of 0.277 than FET D3, will but insignificant with a p-value of >0.05, while the FET D5 laser stage method has a chance of 0.143 significantly higher than FET D3 with a p-value of <0.05.

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