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Journal La Lifesci
Published by Newinera Publisher
ISSN : 27211304     EISSN : 27211207     DOI : https://doi.org/10.37899/journallalifesci
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology
International Journal La Lifesci is peer reviewed, open access Academic and Research Journal which publishes Original Research Articles and Review Article editorial comments etc in all fields of life sciences including Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology, Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 240 Documents
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Alterations in VKORC1 Gene and Cardiac Enzymes in Acute and Chronic Congestive Heart Failure Dhuha Abdullah Abbas
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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Abstract

Congestive heart failure (CHF) is a structural and functional cardiac disorder that activates compensatory mechanisms, including neurohormonal responses and circulatory adjustments, which initially preserve perfusion but later contribute to systemic and pulmonary congestion. This study examined the effect of acute and chronic CHF on VKORC1 gene overexpression and measured serum cardiac enzymes, namely troponin I (cTn I), creatine kinase MB (CK MB), lactate dehydrogenase (L LDH), and myoglobin (MYO MB), at each disease phase. The study included 40 CHF patients, consisting of 20 with acute CHF (ACHF) characterized by severe sudden cardiac symptoms and 20 with chronic CHF (CCHF) showing gradual progression, along with 20 healthy participants as a negative control group. Venous blood samples were collected for molecular analysis using quantitative PCR and for serological assessment of cardiac enzymes using quantitative ELISA. Statistical significance was determined using GraphPad Prism at p < 0.05. The results showed that, across all groups, the GG genotype frequency was significantly higher than GA and AA. However, allele frequency analysis of VKORC1 (-1639G>A) rs9923231 revealed significantly increased GA and AA frequencies in both ACHF and CCHF patients. Serologically, cTn I, CK MB, L LDH, and MYO MB levels were significantly elevated in both patient groups compared with controls. Between ACHF and CCHF, only CK MB was significantly higher in CCHF, while cTn I, L LDH, and MYO MB showed no significant difference. This appears to be the first Iraqi study linking VKORC1 with CHF phases, suggesting its potential as an indicator of both acute and chronic CHF, although further investigation is needed.
Installation Performance Evaluation Domestic Wastewater Treatment Plant in Fulfil Minister of Environment and Forestry Regulation No. 11 of 2025 Irfan Syarifandi; Natsar Desi; Muhammad Chaerul; Erniati Erniati; A. M. Yusuf Harun; Mahmud Mahmud
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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Abstract

Wastewater management domestic is aspect crucial in control pollution environment. especially in developing areas like Merauke Regency. Research This aim evaluate performance Installation Domestic Wastewater Treatment Plant ( WWTP) in Housing District Ulilin in fulfil standard quality Minister of Environment and Forestry Regulation No. 11 of 2025. Research method use approach quantitative descriptive – evaluative with design studies case. Taking sample carried out at the inlet and outlet of the IPAL during September–October 2025 using grab sampling method. with Analysis of TSS, pH, BOD₅, COD, oil and fat, NH₃-N, and total coliform parameters based on Standard Methods (APHA, 2017). The results show that characteristics influence majority exceed standard quality. especially in the parameters BOD₅ (33.4 mg/L), COD (122.5 mg/L), TSS (35.5 mg/L), oil and fat (6.15 mg/L), NH₃-N (13.1 mg/L), and total coliform (3.59×10³ MPN/100 mL). After processing. all effluent parameters fulfil standard quality with level 100% compliance Efficiency elimination show very good performance. with decline significant on BOD₅ (83.38%), COD (84.04%), TSS (49.30%), and NH₃-N (93.13%). High safety margin to standard quality show system own capacity adequate and resilient reserves to fluctuations burden polluters. In overall. domestic wastewater treatment plant District Ulilin operate effective. stable. and feasible in support protection quality environment in a way sustainabl.
The Influence of Sociodemographic Factors on Food Waste Creation Raisa Maharani; Maswadi Maswadi; Dewi Kurniati
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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Abstract

Food waste has emerged as a global issue of growing international concern, with developing countries striving to identify effective strategies to address the problem of food-related waste. In this study, food waste refers specifically to edible household food residues that are discarded despite being fit for consumption. This research aims to analyze the generation of food waste in Singkawang City and to examine the relationship between sociodemographic factors and the amount of food waste generated. The household sampling technique employed in this study was proportionate stratified random sampling, while the selection of individual family member respondents within each household was conducted using incidental sampling. The analytical methods applied include descriptive analysis, food waste quantification, and multiple linear regression analysis. The findings indicate that the highest level of food waste generation occurred in Singkawang Tengah District, producing the largest volume of waste (28,370 kg/year), while the lowest generation was recorded in Singkawang Utara District (7,140 kg/year). Furthermore, the results of the analysis examining the relationship between sociodemographic factors and food waste generation demonstrate that, simultaneously, sociodemographic variables (age, gender, income, education level, occupation, and number of household members) significantly influence the dependent variable (food waste generation). Partially, however, age, gender, education level, and occupation were found not to have a significant effect on food waste generation.
The Relationship Between CRP Level and Age in Salmonella Typhi Patient Haider Abdul-Moneem Al-Mothafer
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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Abstract

Typhoid fever has increased in some populations, hence the current study, aimed to evaluate the prevalence of salmonella seropositivity and other relevant factors such as sex, age, and CRP positivity among febrile patients, especially among the patients estimated to be suffering with typhoid fever located at Al-Najaf province in Iraq. The study comprised 79 febrile patients who presented for intestinal complaints at Al-Hakeem General Hospital, AL Sajad Hospital, and AL Najaf Hospital’s serological departments within Al-Najaf province between October 2024 and January 2025. Those were between 1 and 75 years of age. This study employed rapid testing modalities for the qualitative detection of typhoid infection and assessment of serum CRP levels. Among the 79 febrile patients screened, 41 (51.9%) were female, 38 male (48.1%) was assessed. On typhoid seropositivity, 23 individuals (29.2%) tested positive, and 56 (70.8%) negative, but no differences between gender were found (P=0.36). Age distribution of febrile patients was further classified into 5 groups, 1-15 years, 16-30 years, 31-45 years, 46-60 years, 61-75 years. Notably, the greatest prevalence occurred at a range of 1 to 15 years of age (27.8%), then in the 16-30 year group (25.3%). Among those diagnosed as positive for Salmonella seropositivity, it was reported that those ages 61-75 had a higher positivity rate of 12.7%, indicating a statistically significant difference (P=0.001).
The Importance of Sago Forest for Local Food Security and Climate Change Management on Small Island Marcus Luhukay; Aryanto Boreel; Marthina Tjoa; Gun Mardiatmoko
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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Abstract

Small Island worldwide are highly vulnerable to local food security and climate change. Therefore, appropriate spatial planning is needed to prevent the massive conversion of forest to non-forest areas, including sago, mangrove, and other natural forest on these small Island. This study used a method comprising a variety of activities, including field surveys, drone mapping, and interviews with sago owners and processors. The results showed that Ihamahu Village, on the small island of Saparua (Eastern Indonesia), had a sago forest area of ​​82.08 hectares with an average potential wet starch production of 450 kg per tree and a dry sago starch yield of 443 tons. This production enables local communities to process sago for household use and sell to the Saparua wholesale market. As sago has traditionally been a local food, the community continues to conserve forest in the region. In other words, sago potential has become a primary source of food security for local communities. With the ongoing conservation, the forest ecosystem is increasingly protected. The biomass content, especially of Mature Felling sago trees, was 30.33 tons per hectare, and this biomass would be even greater with the inclusion of Non-Mature Felling sago trees. This indicates that the sago forest ecosystem is capable of absorbing Greenhouse gas (GHG) emissions, thereby playing a significant role in mitigating climate change.
Concept of Coastal Abrasion Disaster Risk Management Haslimin Haslimin; Natsar Desi; Muhammad Chaerul
Journal La Lifesci Vol. 7 No. 2 (2026): Journal La Lifesci
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This study aims to analyze the hydro-oceanographic characteristics, identify the natural and anthropogenic factors causing coastal abrasion, assess the level of abrasion impact, and formulate an appropriate coastal abrasion management concept in the Batauga coastal area, South Buton Regency. A descriptive field-survey approach supported by hydro-oceanographic analysis and qualitative assessment was employed. Primary data were collected through bathymetric measurement, tidal observation, current measurement, field observation, documentation, and semi-structured interviews with relevant stakeholders. Bathymetric data were obtained using a GPS-integrated echosounder, tidal data were analyzed using the least squares method, and current velocity was calculated based on buoy displacement and validated with a digital current meter. The results show that the Batauga coast has a gentle bathymetric profile, allowing wave energy from the Flores Sea to reach the shoreline with limited attenuation. The mixed prevailing semidiurnal tidal pattern expands the active intertidal zone, while longshore currents contribute to sediment transport and sediment imbalance. In addition, illegal sand mining significantly accelerates abrasion by reducing sediment volume and altering seabed morphology. The level of abrasion impact is categorized as high, as indicated by shoreline retreat, seabed degradation, scouring around coastal structures, infrastructure vulnerability, and ecosystem damage. Therefore, a hybrid coastal protection system combining structural measures, non-structural strategies, vegetation rehabilitation, sediment stabilization, and strict control of sand mining activities is recommended for sustainable abrasion management in Batauga.
Organoleptic Quality Characterization of Cookies with Eggshell, Kepok Banana Flour, Arrowroot Starch Martha Arum Nugraheni; Netta Meridianti Putri; Farida Farida
Journal La Lifesci Vol. 7 No. 3 (2026): Journal La Lifesci
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v7i3.5592

Abstract

The aim of this research is to study the effect of adding egg shell flour on the panelists' level of preference for kukis substituted for kepok banana flour and arrowroot starch. This research used a Completely Randomized Design (CRD), with treatments consisting of differences in the composition of the addition of egg shell flour (10%, 15%, and 20%), and the proportion of arrowroot starch: kepok banana flour (100%: 0%, 50%: 50%, 0%:100%). The research results showed that the product with the highest average color preference was F2 with ingredients of 15% egg shell flour, 85% arrowroot starch. The product with the highest average preference for aroma, taste and texture is F3 with ingredients of 20% egg shell flour, 80% arrowroot starch. The cookies made from eggshell flour that were most liked by the panelists from all hedonic aspects (texture, taste, color and aroma) were cookies with a proportion of 80% arrowroot starch, 0% kepok banana flour and the addition of 20% eggshell flour (F3). with an average color preference of 4.13 (like), aroma of 4.05 (like), taste of 4.13 (like), and texture of 4.03 (like).
Chemical Quality of Drinking Water from Community Wells Adelina Siregar; Johannis P. Haumahu; Elizabeth Kaya; June Putinella; Robby G. Risamasu; Rudy Soplanit
Journal La Lifesci Vol. 7 No. 3 (2026): Journal La Lifesci
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Water is a primary necessity for human life, and its quality directly affects public health and community welfare. In Wayame Village, Ambon City, shallow wells are still used by the community as a source of water for daily needs, including drinking water. This study aimed to evaluate the chemical quality of shallow well water by comparing laboratory test results with the drinking water quality standards established by the Ministry of Health of the Republic of Indonesia. This study employed a descriptive quantitative method. A total of eight shallow well water samples were collected from community wells in Wayame Village using purposive sampling. The chemical parameters analyzed included pH, fluoride, total chromium, cadmium, nitrite, nitrate, iron, hardness, chloride, manganese, lead, and free chlorine. Laboratory analysis was conducted at the Provincial Health Laboratory of Maluku, and the results were compared with the Regulation of the Minister of Health of the Republic of Indonesia Number 492/Menkes/Per/IV/2010. The results showed that most chemical parameters were below the permissible limits for drinking water. Fluoride, total chromium, cadmium, nitrite, nitrate, iron, hardness, chloride, manganese, and lead were within the required standards. However, several samples showed pH values below the minimum standard, indicating slightly acidic water conditions. In addition, free chlorine levels in all samples were below the recommended range, indicating limited residual disinfection capacity.
Biochemical Disturbances and Profile of Lipid in Chronic Renal Failure Patients on Maintenance Hemodialysis Raghda M. Ameer
Journal La Lifesci Vol. 7 No. 3 (2026): Journal La Lifesci
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Abstract

Water is a primary necessity for human life, and its quality directly affects public health and community welfare. In Wayame Village, Ambon City, shallow wells are still used by the community as a source of water for daily needs, including drinking water. This study aimed to evaluate the chemical quality of shallow well water by comparing laboratory test results with the drinking water quality standards established by the Ministry of Health of the Republic of Indonesia. This study employed a descriptive quantitative method. A total of eight shallow well water samples were collected from community wells in Wayame Village using purposive sampling. The chemical parameters analyzed included pH, fluoride, total chromium, cadmium, nitrite, nitrate, iron, hardness, chloride, manganese, lead, and free chlorine. Laboratory analysis was conducted at the Provincial Health Laboratory of Maluku, and the results were compared with the Regulation of the Minister of Health of the Republic of Indonesia Number 492/Menkes/Per/IV/2010. The results showed that most chemical parameters were below the permissible limits for drinking water. Fluoride, total chromium, cadmium, nitrite, nitrate, iron, hardness, chloride, manganese, and lead were within the required standards. However, several samples showed pH values below the minimum standard, indicating slightly acidic water conditions. In addition, free chlorine levels in all samples were below the recommended range, indicating limited residual disinfection capacity.
Literature Review: Factors Affecting White Rice Glucose Levels Intan Nur Hidayati; Dwi Sarbini; Eni Purwani; Pramudya Kurnia
Journal La Lifesci Vol. 6 No. 2 (2025): Journal La Lifesci
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Abstract

The high level of rice consumption in Indonesia is due to the belief that people have not eaten if they have not consumed white rice. Based on data from the BPS (2023), Indonesian people's rice consumption reached 31.54 million tons. The biggest content of white rice is carbohydrate content. Carbohydrates are broken down into glucose when they enter the body. White rice has higher glucose levels than brown rice, namely 25.40 per 100 grams (Diyah et al., 2018). According to Susanti & Bistara (2018), most diabetes mellitus sufferers experience hyperglycemia because they tend to consume foods with high glucose. Factors that influence white rice glucose levels are storage temperature, storage time, rice variety and cooking method. The aim of this research is to examine articles regarding factors that influence white rice glucose levels. The research method used is a literature review which examines articles from 2013-2023 of Google Scholar, Garuda, PubMed, Science Direct and Semantic Scholar with indexes Sinta 1-5 or Scopus Q1-Q4. Key words used are kadar glukosa, suhu penyimpanan, waktu penyimpanan, metode pemasakan, rice cooker, magicom, kukus, panggang, bakar, varietas beras putih, nasi putih, storage temperature, storage time, cooking method, steamed roasted, roasted grilled, white rice varieties, glucose content and white rice. Articles were selected using the PRISMA method. From the results of the literature review, it shows there is an influence of storage temperature (50%), storage time (25%), rice variety (50%) and cooking method (67%) on white rice glucose levels.

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