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Sutiman Bambang Sumitro
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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 16 No 2 (2011): June 2011" : 15 Documents clear
KAJIAN PRA-KLINIS PEMBERIAN ORAL BUBUK KERING-BEKU SUSU KEDELAI YANG DIFERMENTASI OLEH Lactobacillus plantarum AP1 DAN Sphingobacterium SP. TB17 PADA TIKUS Achmad Dinoto; Rita Dwi Rahayu; Sri Purwaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/305

Abstract

To investigate the effects of oral administration of freeze-dried soymilks fermented by Lactobacillus plantarum AP1 and Sphingobacterium sp. TB17, in preclinical study groups of male Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (CD), basal diet supplemented with 3% (w/w feed) freeze-dried unfermented soymilk (CN), basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by L. plantarum AP1 (CL), and basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by Sphingobacterium sp. TB17 (CK). Our result showed a reduction of serum cholesterol level up to 15% in rats CB after three weeks consuming soymilk fermented by L. plantarum AP1. This yield was relatively higher than that of rats CK consuming soymilk fermented by Sphingobacterium sp. TB17 to reduce serum cholesterol only up to 5%. Although unfermented soymilk (CN) also showed the hypocholesterolomic effect to rats, the reduction of cholesterol serum level was lower than that of rat CL. There were no signifi cant increased in blood glucose and body weight of rats as impact of freeze-dried fermented soymilk. Interestingly, although feed intake tends to increase during fermented soymilk consumption, the body weight of rats did not increase. In general, this study indicated that L. plantarum AP1 soymilk is a promising beneficial food product for the health.
KOMPOSISI KIMIA DAN AKTIVITAS ANTIBAKTERI MINYAK ATSIRI Piper gibbilimbum C. DC.: PIPERACEAE Praptiwi; Yuliasri Jamal; Ahmad Fathoni; Ary P. Keim
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/306

Abstract

Distillation of Piper gibilimbum leaves by water distillation yielded 0.85% light yellow essential oil. Result of GC-MS analyses showed that P. gibilimbum essential oil contained 72 chemical compounds with major components 3-carene (40.64%), β -pinene (8.47%), cineol (6.19%), α-copaene (5.47%) and α-guaiene (9.30%). Antibacterial test with disc paper method indicated that the essential oil at 50% concentration inhibited the growth of Staphylococcus aureus, Bacillus subtilis, Streptococcus mutans, Streptococus viridans, Escherisia coli and Shigella flexneri.
PEMEKATAN EKSOPOLISAKARIDA (EPS) DARI KULTUR BAKTERI USUS DALAM BIOMASSA SAGU (Metroxylon SP.) MELALUI MODUL MEMBRAN ULTRAFILTRASI CROSS-FLOW (UFCF) Agustine Susilowati; Aspiyanto; Achmad Dinoto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/307

Abstract

Cross-flow ultrafiltration (UF) membrane is a main separation and/or concentration techniques influenced by operation condition (flow rate, temperature, pressure, time) and molecular weight (MW). The goal of this experiment was to find out the effect of concentration time of Lactobacillus sp. FU 0811 and Enterobacter sp. FU 0813 cultures in biomass of sago (Metroxylon sp.) on UF membrane performance (flux value and rejection), and retentate/concentrate composition as carrier agent of oral insuline activated compounds. Concentration process was performed through cross-flow UF membrane module at pump motor frequency of 20 Hz (flow rate of 7.5 L/minute), room temperature and operation pressure of 5 bar for each 0, 15, 30 and 45 minutes. The result of experiment showed that retentate/concentrate had better composition and total microbes count than that permeate. Based on EPS as reduction sugar, time needed to concentrate Lactobacillus sp. FU 0811 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar, total solids, total acids concentrations, and total microbes count of 4.2 mg/mL, 0.2443% and 0.013%, and 4.61 log CFU/mL. While, permeate flux value was 43.66 L/m2.hour, and rejections of reduction sugar, total solids, total microbes and total acids on membrane were 85.7–90.6%, 96.6–99.1%, 100% dan 12.9–40.2%. Whereas, EPS as reduction sugar, time needed to concentrate Enterobacter sp. FU 0813 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar, total solids, total acids concentrations, and total microbes count of 0.18 mg/mL, 0.0901% and 0.013%, and 4.806 log CFU/mL. While, permeate flux value was 40.83 L/m2.hour, and rejections of reduction sugar, total solids, total microbes and total acids on membrane were 0–68.75%, 81.4–86.8%, 100%, dan 17.6 – 30.3%. Identification of retentate/concentrate from Lactobacillus sp. FU 0811 culture showed that its presence of EPS compounds as Dextran (MW 10,500 Dalton, Da.), Maltohexaose (MW 342.3 Da.) and Lactose (MW 990.86 Da.)
KEANEKARAGAMAN BAKTERI PENGHASIL EKSOPOLISAKARIDA ASAL SALURAN CERNA MANUSIA Achmad Dinoto; Sugiyono Saputra; Agustinus Joko Nugroho; Rita Dwi Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/308

Abstract

Exopolysaccharide (EPS) is a polysaccharide that is excreted by cell as metabolites. Bacterial EPS has been widely used in food and drug industries. In order to find natural products for foods and drugs, the study was conducted to know the diversity of EPS-producing bacteria isolated from human gastrointestinal tracts. As much of 30 bacterial strains were collected from two healthy people and seventeen of collected isolates were investigated for the capability of producing EPS. As the results of identification by partial 16S ribosomal DNA analysis, those seventeen EPS-producing bacterial strains were closely related with already known bacteria (range between 86 to 100% similarity) and could be classified into seven genus, i.e.: Lactobacillus spp. (9 strains), Enterobacter sp. (2 strains), Escherichia sp. (2 strains), Klebsiella sp. (1 strain), Cronobacter sp. (1 strain), Staphylococcus sp. (1 strain), and Alteromonas sp. (1 strain). In addition, member of Lactobacillus group which have closest relationship with species L. plantarum (7 strains) dan L. fermentum (2 strains) were known to dominate the collection of culturable EPS-producing bacteria from human gastrointestinal tracts.
PENGARUH PUPUK ORGANIK HAYATI YANG MENGANDUNG MIKROBA BERMANFAAT TERHADAP PERTUMBUHAN DAN HASIL PANEN TANAMAN SEMANGKA SERTA SIFAT BIOKIMIA TANAHNYA PADA PERCOBAAN LAPANGAN DI MALINAU-KALIMANTAN TIMUR Sarjiya Antonius; Dwi Agustiyani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/309

Abstract

In field experiments during dry season 2010, the effects of biofertilization on growth of fruit yield Semangka (Citrullus lanatus) and of biochemical properties of soil were investigated. The experimental design was Randomly Block Design (RBD) with four treatments and five replications. The treatments were biofertilizer only; chemical fertilizer only; biofertilizer plus a half dose of chemical fertilizer, and control. The application of biofertilizer which was a mixture of Pseudomonas sp., Bacillus sp. and Streptomyces sp and chemical fertilizers (only 50% of the recommended dosage of NPK) increased vegetative growth (plant height, number of branches) compared to chemical fertilizer treatments only. An addition of biofertilizer with the chemical fertilizer also increased the biochemical properties as well as the yield of C. lanatus compared to other treatments. The possible role of biofertilizer on improving soil enzymatic activities, vegetative growths and yield will be discussed more detail in the paper.

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