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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 9 Documents

Search results for , issue "Vol 18 No 2 (2013): June 2013" : 9 Documents clear

HUMAN SPERM PROTEIN 116 KDA: A CANDIDATE ANTIGEN FOR IMMUNOCONTRACEPTION TECHNOLOGY Umi Lestari; Aulanni’am Aulanni’am; Basuki B. Purnomo; Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Spermatozoa membrane antigenic protein utilized as the substance candidate of immunocontraception has important criteria, that are only expressed in spermatozoa, cannot be found in the other tissue, and also should not have kinase activities. The observations done finally conclude 116kD, a sperm head’s protein is within the above criteria. It’s antigenic characters are shown with ability of it’s polyclonal antibody to bind the human spermatozoa, and interestingly this polyclonal antibody enable to block fertilization of other species in an in-vitro test experiments. This reseach also acquired that 116 kDa protein is specific and exists only in spermatozoa, and not in the other tissue such as spleen, kidney, pancreas, and epidydimic. This 116 kDa protein distribute along the whole area of the sperm, but mostly accumulated around the head up to the the neck. The imunohistological staining of the testis also indicate that this protein exists in the spermatid of the testes, but not be found in other somatic tissues, and it’s antibody is evidently was recognised by the goat and cow spermatozoa membrane protein resulted in blocking the fertilization of both species respectively. And the conclussion, 116 kD is Non-kinase protein specific only exists in the area of the head of human spermatozoa. It is therefore believed to be adequate candidate for antigen substance of immunocontraception technology.

GENETIC DIVERSITY OF Lactobacillus spp. OF NATURAL ETHAWAH GOAT MILK-FERMENTED WAS DETERMINED BY USING 16SRDNA WITH DDGE ANALYSIS Nur Kusmiyati; Nur Hidayat; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

phoresis (DGGE) analysis. Goat and bovine (as negative control) milk naturally fermented among 24hour until 6 days. Morphological and biochemistry of bacteria were characterized by standard methods. The total DNA of bacteria were isolated using alkali lysis, PCR amplification was carried out using 3 pairs of specific primers, DNA-amplified using DGGE and then to determine the relationship among Lactobacteria using NTsys package software V2.0. Phenotypical and biochemical study showed that the 11 strains are belonging to genus Lactobacillus. The dendogram results show all of isolates had similarity characters with genus Lactobacillus around 56-76%. According to morphological and DDGE profiles, we were identified that bacteria isolate of goat milk-fermented are K1A, and K3A are L. casei, and bacteria isolate K3B is L. plantarum.

ISOLATION OF Pichia manshurica PROTOPLAST FROM Dahlia sp. PLANT Wijanarka Wijanarka; Endang Sutariningsih Soetarto; Kumala Dewi; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Isolation of protoplasts is an important step in the fusion process. Protoplasts are cells that have eliminated the cell wall, but the cell membranes and organs can still function properly. Pichia manshurica is one of indogenous yeast that derived from Dahlia’s plants. The success rate protoplast isolation was determined by various factors, include the age of the culture and the used of lytic enzymes. The purpose of this research is to get the perfect age of yeast culture that is ready to be harvested and also to get the appropriate concentration of Glucanex lytic enzymes which used for protoplast isolation. The yeast of Pichia manshurica grown on YPD broth medium and growth observed in turbidimetry. Observation of the growth of yeasts performed every 6 hours for 42 hours. Glucanex lytic enzyme concentration used for the isolation of protoplasts is 0 mg / mL (L0 = control), 2 mg / mL (L2) and 4 mg / mL (L4). The results showed that the age of the culture is right and ready for harvest at the age of 24 hours and Glucanex lytic enzyme concentration of 4 mg / mL (L4) is able to produce the best of protoplasts at 7.2 x 1010.

PATCHOULI ALKOHOL ISOMERS POGOSTEMON HERBA PREDICTED VIRTUALLY Sentot Joko Raharjo; Chanif Mahdi; Nurdiana Nurdiana; Wolfgang Nellen; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

The aim of our research is predicting the alpha-patchouli alcohol isomer Pogostemon Herba as inhibitors cyclooxygenase (COX-1 and COX-2) isoenzymes. The data for the alpha-patchouli alcohol isomer (CD521903, CD442384, and/or CD6432585) Pogostemon Herba were explored from the pubchem database. Molecular interaction studies with COX-1 and COX-2 from mouse were done using the molecular docking tools Hex 6.12 and LeadIT2 Bisolve. The analysis of the alpha-patchouli alcohol compounds of patchouli oil showed that alpha-Patchouli alcohol (CD521903) binds to COX-1 at active sites including: LEU223B, ASP228B, LEU237B, ARG 332B, TRP 138A, GLU 139A, SER 142A, ASN 143A, and the interaction to COX-2 at active site including: GLN 289B, GLU 290B, ARG 222B, LYS 211B, THR 212B, HIS 214B, ASN 382B, HEM682B, GLN 454B, HIS 386B, TRP 387B, HIS 388B, VAL 274B, GLN 203B, VAL 291B, VAL 295B. The interaction hydrogen bond energy between alpha-patchouli alcohol: (CD521903-COX-1 complexes (-4 kJ/mol) and CD521903-COX-2 complexes (-8 kJ/ mol) by LeadIT2 Biosolve. This suggests alpha-patchouli alcohol CD521903 as candidate for a selective COX-2 inhibitor. These in silico data need further analyses of biological function activity.

CONTAMINATION OF Pb, Cd, Cu, Zn IN BLACK POMFRET (Formio niger) AND TONGUE SAND (Cynoglossus lingua) FISH CAUGHT FROM THE WESTERN PART OF MADURA STRAIT AND ITS SAFE LIMITS CONSUMPTION Acivrida Mega Charisma; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Contamination of Pb, Cd, Cu, Zn in the flesh on the two types of fish consisting pelagic fish include Black pomfert (Formio niger) whereas among other demersal fish include Tongue sand (Cynoglossus lingua), was conducted in March-December 2013, which was taken from the western part of Madura Strait (Gresik-Ujung pangkah, Surabaya-Kenjeran, and Sidoarjo-Sedati). Pb, Cd, Cu, and Zn detected using AAS (Atomic Absorption Spectrometry) brand Shimadzu AA-6200. The highest content of heavy metals, namely Pb in Cynoglossus lingua from the Sedati (5.63ppm±0.05), while a low of Cd in Formio niger from the Ujung pangkah (0.20ppm±0.004). The results show that there is a correlation between heavy metal with fish but not significantly. The content of Pb, Cd, Cu, Zn in 2 fish species originating from all locations stated below the exposure limits for consumption. Safe limit fish consumption of Formio niger is based on the WHO PTWI of 347.43 grams / week and Cynoglossus lingua 191.25 grams / week.

THE EFFECT OF BIOFERTILIZER FUNGI ON CIHERANG RICE GROWTH AT SOME LEVEL OF SOIL SALINITY YB. Subowo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

research about the effect of fungus contained biofertilizer on Ciherang rice that was growth on different level of soil salinity was conducted. One of the effect of global climate changes is the increase of sea water level. It leads to the expansion of sea water submerged land for agriculture. Salt intrution to the agriculture area considerably decrease soil fertility because of the high salinity. Some of microbes especially soil fungi such as Aspergillus sp and Penicillium sp. are able to grow at high salinity environment. Those fungi were also able to degrade lignocellulose, sollubilize in organic phosphate and provide organic phosphat and produce plant growth hormon especially IAA. Such activities benefit to improve soil fertility in high salinity land as a bio-fertilizer.The objective of this research was to know the growth of rice plant that treated with fungus contained bio-fertilizer on land with different level of salinity. The rice were planted in Green house of Cibinong Science Centre, Cibinong.The research was set up as complete random design with five replication. The rice were watered by 5 conditions: 50% of sea water, 100% of sea water, 100% sea water + 2 % NaCl , fresh water + 5 % NaCl and 100% fresh water as the control. Fertilizer was added to the medium twice. Ten grams of fertilizer were used per polybag (10g/7 Kg), 2 weeks after planting and before flowering subsequently. The observed parameters were plant height, number of tiller, leaves colour, biomass dry weight, soil organic carbon content, cellulosic and lignin degrading activities of the fungus, fungus phosphate-solubilizing potency and fungus production of IAA.The watering treatment lead to 5 level of salinity i.e. : 5,93 dS/m (50% sea water), 9,15 dS/m (100% sea water), 10,42 dS/m (sea water + 2% NaCl), 12,43 dS/m (fresh water + 5% NaCl) and 0,74 dS/m (fresh water). The result showed that among those 5 watering condition, the rice grew best on 5,93 dS/m (watering 50% of sea water). This result was considered as the best since the plant height and number of tiller were not significantly different with those of the control. Plants height and number of tiller were 74,4 cm and 11 respectively. On the higher salinity level the plant growth was inhibited. The ability of rice to withstand the high salinity possibly was supported by the fungus activities of providing organic phosphate and IAA growth substance.

DIVERSITY OF THE UNIONID FRESHWATER MUSSELS (BIVALVIA: UNIONIDAE) IN BRANTAS RIVER, EAST JAVA, INDONESIA Moch. Affandi; Leonard Ady Candra; Astra Budi Priatama; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

The aims of this study are to provide data and information about diversity, dominance, and distribution of the Unionidae mussels species in Brantas River, East Java, Indonesia. Mussel samples were collected from 14 sampling stations along the Brantas River, starting from upstream in Tulungagung to downstream in Surabaya (± 170 km) in April-May 2012. The results showed that five species of mussels, namely: Contradens contradens, Elongaria orientalis, Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis were observed in the Brantas River. Contradens contradens was the “dominant” species, followed by Elongaria orientalis as a “common” species, and Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis as “rare” species. Distribution of each species in the Brantas River tends to be concentrated in downstream of the river, mainly located in station 13. According to the IUCN Red List, Rectidens sumatrensis was categorized in “Data Deficient”, while Pseudodon vondembuschianus and Pilsbryoconcha exilis were categorized in “Least Concern”.

THE EFFECT OF BIVALVE Semele sp. MEAT CONSUMPTION ON ESTROGEN LEVEL AMONG THE PEOPLE OF MUNA REGENCY, SOUTHEAST SULAWESI Sjafaraenan Sjafaraenan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

A study on the influence of bivalve Semele sp meat consumption on estrogen level among the people of Muna Regency, Southeast Sulawesi, was conducted in Lasalepa Village, Bonea Subdistrict, Muna Regency, Southeast Sulawesi. This study was aimed to: (1) determine the nutritional content of bivalve Semele sp meat, (2) compare the estrogen levels between women that consume Semele sp meat and those that do not. This study was an experimental study with one group pre-test and post-test. Subjects consisted of 20 women in the age range of 40-55 years old. Subjects consumed raw Semele sp meat in the amount of 400-500 g three times in a week for one month. Blood samples were collected through cubital median vein, before and after bivalve consumption. Protein content was measured by Kjeldhal method, carbohydrate by Anthron method, fat by gravimetry, mineral by AAS, and estrogen by ELISA technique. Study findings indicated that (1) the protein content was 7.182%, carbohydrate 66.87%, fat 6.82%, cholesterol 10mg/dL, calcium 263.385 ppm, cuprum 9.107 ppm, magnesium 28.467 ppm, ferum 1,859 ppm, zinc was undetectable, (2) there was a significant difference in estradiol and estron levels before and after bivalve consumption in the three age groups, 17,022 pg/mL and 51.018 pg/mL, (3) there was no significant difference in estriol level before and after bivalve consumption in the three age groups.

THE DYNAMICS OF DAIDZEIN CHEMICAL COMPOUND FROM YAM TUBER (Pachyrhizus erosus) TUBER AND ITS POTENTIAL IN MYOMETRIUM Cicilia Novi Primiani; Umie Lestari; Mohammad Amin; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Daidzein compounds dynamic and the effect of yam tuber (Pachyrhizus erosus (L)) Urb on myometrium rats model of estrogenic were investigated. Twentyfour of 5 months-old female Sprague Dawley rats were randomly assigned to three groups as followed, control, Daidzein administraton and yam tuber juice administration for 24 days. The administration of yam tuber juice were given by orally using a stomach with gavage tube in the morning. Every 8 h, the rats blood and urine were collected from tail until 24 h. The treatment were continued until 24 days. The result showed that the level of daidzein in blood and urine of rats that administratered by Yam tuber juice is lower than pure daidzein treatment rats every 8 until 24 hours of collection. The results demonstrated that the administration of yam tuber juice significantly myometrium proliferated in rats. It is assumed that genistein and quercetin is estrogenic as found in daidzein so that the miometrium is potentially more effecctive. This study suggest that phytoestrogen compound from Pachyrhizus erosus may offer a potential as natural estrogenic in women.

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

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Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA