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Enggar Patriono
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Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University Palembang-Prabumulih Road KM 32 Indralaya, Ogan Ilir, South Sumatera 30662 Indonesia
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INDONESIA
BIOVALENTIA: Biological Research Journal
Published by Universitas Sriwijaya
ISSN : -     EISSN : 24771392     DOI : https://doi.org/10.24233/biov
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
BIOVALENTIA: Biological Research Journal aims to review and publish the Biological research results from researchers. The range of articles which BIOVALENTIA: Biological Research Journal publishing is the research results in Biology science and applied Biology. Biology science is included Ecology, Biosystematics, Physiology, Developmental Biology, Microbiology, Genetics. Applied Biology is included Biotechnology, and the sciences derived from Biology science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 10 No. 1 (2024)" : 8 Documents clear
The Arthropods Diversity in Chili (Capsicum annum L.) Cultivation in Pagaralam, South Sumatra: Arthropods Diversity in Chili (Capsicum annum L.) Cultivation in Pagaralam, South Sumatra proteksi, Arsi; Gunawan, Bambang; Pratama, Rahmat Pratama; Gustiar, Fitra; Suparman; Hamidson, Harman Hamidson; Pujiastuti, Yulia Pujiastuti; Umayah, Abu Umayah; Tricahyati, Titi Tricahyati; Pradiefta, Muhammad Rafii Pradiefta
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.371

Abstract

Red chili (Capsicum annuum L.) is one of important types of vegetables and also has many varieties that have distinctive colors and shapes, chili is also considerd as one of vegetable that has high economic value. Chili is usually consumed in from of fresh, dried and processed as vegetables and also seasonings, in addition to functioning as a food flavor enhancer. Many obstacles faced by farmers in chili cultivation are plant disturbing organisms such as pests and diseases. Some important pests that commonly attack chili plants are fruit flies (Bactrocera spp), armyworms (Spodoptera litura) and aphids (Aphis gossypii). The purpose of this field practice was to determine the diversity of arthropods and the intensity of pest attacks in Pagaralam City on ten chili fields with different ages. This field practice is expected to provide information about arthropod diversity and attack intensity, especially to farmers as the main actors in agriculture. This insect observation method is carried out using the Diagonal sampling method, Scan sampling (Visual observation) and observing the intensity of the attack. The results of the observations obtained are that the chili farmers have carried out the concept of integrated pest management, but they are not aware of it, and the pests obtained in the field are fruit flies (Bactrocera spp), armyworms (Spodoptera litura) and aphids (Aphis gossypii), and for arthropod diversity it varies on each land.
Participatory fecal collection on wild sumatran elephants' dungs in South Sumatra: The significant role of community involvement Pratiwi, Dian Neli; P. Haryanto, Sugeng; Yanti, Yulianti; Arum, Setiawan; Samsul , Bakri; Winda, Indriyanti; Elly Lestari, Rustiati
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.381

Abstract

Sumatran elephant Elephas maximus sumatranus is a critically endangered species under pressure of habitat fragmentation and human-elephant conflict (HEC) such as in one plantation and/or settlement area next to its natural habitat, Simpang Tiga Abadi, Selapan, South Sumatera.  Due to HEC, local people may experience economic loss and human casualty. Simpang Tiga Abadi is avillage, especially 5 sub-settlements directly close to the production forest area of PT. Bumi Khatulistiwa Mandiri, which is the natural habitat of sumatran elephants. The communities collaborated and company were formed an elephant watch group to monitor and deter sumatran elephants from entering plantation areas and settlements. On the other side, sumatran elephants entering the plantation may give some conservation support as indirect conflict mitigation approaches.   A non-invasive sampling can be done by gathering information on its fecal based genetic diversity and may become one of supporting sumatran elephant conservation efforts.   Under the Center for Applied Science Studies, Sriwijaya University project: "Genetic analysis of the sumatran elephant sub-population in its  habitat pocket in Sugihan- Simpang Heran, South Sumatra", Tropical Forest Conservation Action (TFCA) cycle VIII project, the significant role of local community affected by HEC on gathering information and fecal samples of sumatran elephant entering the PT.   Bumi Khatulistiwa Mandiri plantation was conducted.   Sumatran elephants’ fecal sampling involved the local communities affected by HEC. Its aim is to value the significance of local people’s involvement in finding and collecting dung left by wild sumatran elephants during entering both plantation areas. Data on areas were recorded, and its dung samples were collected for molecular analysis.  Collecting information and mapping the entry points of sumatran elephants were done supported by the Simpang Tiga Abadi community, consisting of sumatran elephant’s entry points, dung presence, individual numbers, and group activities, as well as the damage. Eighteen fecal samples of 5 entry points were collected in 5 days. By doing the assistance, the local communities learnt the benefits and important value of sumatran elephant and its dung.   This research is the first local communities’ indirect step in efforts to conserve sumatran elephants in Simpang Tiga Abadi Village, Selapan, South Sumatra.
MANAGEMENT FOR GAPOKTANHUT LESTARI SEJAHTERA: INITIAL OPTIMIZATION ON ROBUSTA COFFEE DNA AMPLIFICATION FOR MOLECULAR SPECIES CONFIRMATION Elly Lestari Rustiati; Priyambodo; Dian Neli Pratiwi; Eko Agus Srihanto; Muhammad Febriansyah; Andri Wijaya Kusuma; Enny Saswiyanti; Alvin Wiwiet Susanto
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.429

Abstract

Gapoktanhut Lestari Sejahtera, a community engaged in coffee plantation practices within the community forests of the Forest Protection of Register 31 Pematang Arahan, Tanggamus, operates under the forest management unit, KPH Kotaagung Utara, Tanggamus, Lampung.  In line with its commitment to biodiversity governance and the preservation and conservation of forest biodiversity, species confirmation, particularly for its robusta coffee, is deemed necessary. A leaf-based molecular species confirmation was proposed, a method yet to be conducted in Lampung.  Therefore, prior to its molecular analysis, a preliminary study focusing on temperature optimization for robusta coffee DNA extraction for species confirmation was undertaken. This study was conducted under the DIPA BLU Unila Basic Research Grant in 2023, in collaboration with the Biotechnology Laboratory, Lampung Disease Investigation Center.  Robusta coffee leaves were sampled from six forest farmer groups, KTH Bumi Mulyo, Sido Makmur 1, Sido Makmur 2, Sido Makmur 3, Mandiri Jaya, and Murah Rejeki 1, in collaboration with Gapoktanhut Lestari Sejahtera, Semaka, Tanggamus. The DNA extraction process adhered to the Genomic DNA Mini Kit (Plant) protocol (ISO 9001: 2008 QMS). DNA amplification was performed using the Polymerase Chain Reaction (PCR) method, utilizing the Coffea N-methyltransferases gene marker primer.  The DNA analysis on robusta coffee leaf samples from Gapoktanhut Lestari Sejahtera demonstrated a good presence of DNA with an amplification temperature of 55°-60°C. To advance to the subsequent process, optimization of DNA extraction is required to yield improved results.
Growth Evaluation of Backcross Progenies BC2F1 Experiencing Submergence and Non-submergence at BC1F1 and its Parents Jati, Winggi Anggun; Agus Suwignyo, Rujito; Susilawati; Muhardiyanto, Cahya
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.431

Abstract

The utilization of dual-resistant varieties that are resistant to soaking and drought in certain periods is an alternative to rice cultivation in swampy land. Inpara 8 is a variety that is confirmed to have SUB 1 gene, resistant to submerged stress and Inpago 5 is identified to be resistant to drought stress. Crosses were made between Inpara 8 (donor parent) with Inpago 5 (recipient parent), resulting in F1, then F1 was crossed back with Inpago 5 and produced BC1F1, then crossed back with Inpago 5 to become BC2F1. This paper contains an evaluation of the growth of BC2F1, which in the BC1F1 stage experienced submerged stress in the vegetative phase and which did not experience submerged stress. The research was carried out in August 2021 - January 2022 at the Greenhouse of the Department of Agricultural Cultivation, Faculty of Agriculture, Sriwijaya University. The results showed that in the vegetative phase, progeny BC2F1 TR has an average value of the highest per plant (113.64 cm) and the average per plant height Inpara 8 (3.93). In the generative phase, the fastest flowering time on the accession of BC2F1 TR (68 days), the fastest harvest time on Inpara 8 (122.93 days), while for the amount of grain per panicle, the amount of grain per clump, and dry weight on Inpago 5 (529.60 g, 2648 g, and 11.62 g). The average yield of BC2F1 progeny is more similar to Inpago 5 as the recipient parent. Both progeny have the same Sub 1 gene in them and can survive to produce, but Accessions that have experienced previous submerged stress are more vulnerable to growth so they have an impact on the resulting production.
Growth Evaluation of Selfcrossed Progenies BC1F2 Experiencing Submerged and Non-submerged at BC1F1 and its Parents Maulidya, Azzah; Agus Suwignyo, Rujito; Susilawati; Muhardiyanto, Cahya
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.432

Abstract

Abstract: A non-tidal swamp can increase rice cultivation and production, but its use is still dependent on natural conditions, making plants vulnerable to submergence and drought stress. Developing dual-tolerant varieties is essential for rice cultivation in non-tidal swamps. Inpara 8 carries the SUB1 gene, providing tolerance to submergence stress, while Inpago 5 is tolerant to drought stress. This study aimed to evaluate the growth of BC1F2 progeny from a cross between Inpara 8 and Inpago 5 under submerged stress, along with their parents (Inpago 5 and Inpara 8). The research was conducted in the greenhouse of the Agriculture Faculty at Sriwijaya University at coordinates 3°13'11.1"S 104°38'49.4"E from July 2021 to January 2022. Inpago 5 exhibited the highest average plant height (145.81 cm), the most tillers (4.73), the most productive tillers (3.87), the highest total grain per clump (720.87 grains), the heaviest clump grain weight (15.86 g), and the lowest percentage of empty grains (33.47%). The donor parent Inpara 8 had the highest average total grain per panicle (192.08) and the highest dry weight (20.69 g). BC1F2 Submerged progeny showed the highest average 1000-grain weight (25.26 g) and the fastest harvest age (114.50 DAP). The BC1F2 self-cross progeny subjected to submergence stress displayed characteristics similar to its parents, with no significant differences in observed parameters.
Molecular and Epigenetic Impacts of Pesticides on the Female Reproductive System: A Review Kamila Alawiyah; Dian Anggraini; Wildan Mubarok; Dwi Hardestyariki
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.434

Abstract

Pesticides, primarily originating from agricultural activities, have raised environmental and health concerns due to their suspected effects on the female reproductive system. Despite their widespread application, a significant research void exists concerning their implications for human female reproductive health. This review provides comprehensive information on the adverse effects of pesticides on female reproduction, focusing on their molecular and epigenetic mechanisms. In general, these adverse outcomes include ovarian dysfunction, hormonal irregularities, the onset of endometriosis, and an increased risk of stillbirths. From a mechanistic perspective, pesticides can interfere with the endocrine system, catalyze the formation of reactive oxygen species, and induce oxidative stress. At the molecular level, certain pesticides can act as hormone mimics, disrupting the normal hormonal balance. Moreover, they can trigger epigenetic shifts, leading to alterations in non-coding RNAs, histone configurations, and DNA methylation patterns. These epigenetic alterations not only have an immediate impact but can also be passed on to subsequent generations. Highlighting these concerns, this review underscores the urgent need for stringent regulations and aims to catalyze future in-depth studies on the relationship between pesticides and female reproductive health.
Monitoring the Use of Synthetic Pesticides for Pest and Disease Control of Rice Plants in Tidal Marshlands Wagi, Wagiyanti; Hamidson, Hamidson; Suwandi, Suwandi; Umayah, Umayah
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.435

Abstract

The majority of people in tidal marshland areas are farmers. Many of the crops grown by the community are food crops such as rice. Rice is one of the food crop commodities that has high economic value. In cultivating rice plants to improve the quality of production, farmers on tidal land currently use chemical pesticides as the most effective alternative source in controlling pests and diseases. This study aims to determine the various types of pests and diseases in rice plants, and the types of synthetic pesticides used by farmers in increasing awareness of the environment from good and proper agricultural practices. The research was conducted in 2023 during the rice season. The method of implementation was carried out by surveying 40 rice farmers in Air Salek District, Banyuasin Regency, namely interviewing according to the questionnaire. The results of the monitoring and interviews showed that the pests and diseases that attacked rice plants were False White Pest (Cnaphalocrosis medinalis), Rice Stem Borer, Brown Stem Leafhopper (Nilaparvata lugens), Armyworm (Spodoptera litura), Leaf Blast, and Neck Blast. There are two types of synthetic pesticides used to control pests and diseases, namely insecticides made from the active ingredient Klorpiripos with a percentage of 55% and 85% fungicides made from the active ingredient Trifloxystrobin 25%, Tebukunazol are in demand by farmers. In the application of synthetic pesticides farmers do not meet the standards due to the low education of farmers.
Effectiveness Formulation of Tobacco and Tannin Fermentation Extract against Aphids (Aphis gossypii Glover) on Curly Red Chilli Latutoibin, Risal; Suwandi, Suwandi; Irsan, Chandra; Harman, Hamidson; Suparman, Suparman; Ahmad , Muslim
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.436

Abstract

Aphids is one of the main pests that attack curly red chili plants. Bioinsecticides can be an alternative to synthetic insecticides in controlling aphids. Tobacco leaf extract and tannin can act as insecticides against various types of insect pests. The aim of this study was to determine the effectiveness of formulations containing fermented mixtures of tobacco leaves and tannin as bioinsecticides against aphids on curly red chili plants. The efficacy experiment was conducted in a greenhouse arranged in a completely randomized design (CRD) with 5 formulation concentration treatments, namely 10%, 5%, 2.5%, 1.25%, and 0% (water), with 5 replications. The treatment was carried out by directly spraying the formulation liquid onto the underside of leaves infested with aphids. The results showed that the tobacco and tannin formulation treatments significantly reduced aphid populations, particularly at the 5% and 10% concentration treatments. The efficacy of population suppression reached 92% after 3 days for the 10% concentration and suppressed the population by 93% after 5 days of application for the 5% concentration. After 7 days of application, the efficacy of aphid population suppression was 99% and 100%, respectively, for the 10% and 5% concentrations. The LT50 values for population suppression occurred after 1.18 and 2.67 days for the 10% and 5% concentrations, respectively. The fermented mixture of tobacco and tannin formulations has the potential to be used as a bioinsecticide for controlling aphids.

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