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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis Jurnal Biogenerasi BIO-EDU: Jurnal Pendidikan Biologi Biofarm : Jurnal Ilmiah Pertanian BIOVALENTIA: Biological Research Journal Kenanga : Journal of Biological sciences and Applied Biology Journal Of Agriculture Sciences (Agrosci) Journal of Community Service (Abdisci)
Pratiwi, Dian Neli
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK Virnarenata, Elsa; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.49 KB) | DOI: 10.24233/biov.7.1.2021.175

Abstract

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.
Participatory fecal collection on wild sumatran elephants' dungs in South Sumatra: The significant role of community involvement Pratiwi, Dian Neli; P. Haryanto, Sugeng; Yanti, Yulianti; Arum, Setiawan; Samsul , Bakri; Winda, Indriyanti; Elly Lestari, Rustiati
BIOVALENTIA: Biological Research Journal Vol. 10 No. 1 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.1.2024.381

Abstract

Sumatran elephant Elephas maximus sumatranus is a critically endangered species under pressure of habitat fragmentation and human-elephant conflict (HEC) such as in one plantation and/or settlement area next to its natural habitat, Simpang Tiga Abadi, Selapan, South Sumatera.  Due to HEC, local people may experience economic loss and human casualty. Simpang Tiga Abadi is avillage, especially 5 sub-settlements directly close to the production forest area of PT. Bumi Khatulistiwa Mandiri, which is the natural habitat of sumatran elephants. The communities collaborated and company were formed an elephant watch group to monitor and deter sumatran elephants from entering plantation areas and settlements. On the other side, sumatran elephants entering the plantation may give some conservation support as indirect conflict mitigation approaches.   A non-invasive sampling can be done by gathering information on its fecal based genetic diversity and may become one of supporting sumatran elephant conservation efforts.   Under the Center for Applied Science Studies, Sriwijaya University project: "Genetic analysis of the sumatran elephant sub-population in its  habitat pocket in Sugihan- Simpang Heran, South Sumatra", Tropical Forest Conservation Action (TFCA) cycle VIII project, the significant role of local community affected by HEC on gathering information and fecal samples of sumatran elephant entering the PT.   Bumi Khatulistiwa Mandiri plantation was conducted.   Sumatran elephants’ fecal sampling involved the local communities affected by HEC. Its aim is to value the significance of local people’s involvement in finding and collecting dung left by wild sumatran elephants during entering both plantation areas. Data on areas were recorded, and its dung samples were collected for molecular analysis.  Collecting information and mapping the entry points of sumatran elephants were done supported by the Simpang Tiga Abadi community, consisting of sumatran elephant’s entry points, dung presence, individual numbers, and group activities, as well as the damage. Eighteen fecal samples of 5 entry points were collected in 5 days. By doing the assistance, the local communities learnt the benefits and important value of sumatran elephant and its dung.   This research is the first local communities’ indirect step in efforts to conserve sumatran elephants in Simpang Tiga Abadi Village, Selapan, South Sumatra.
Supporting Environmental Resilience through Biological Approach: Initial Step for Building Robusta Coffee Germplasm Database Rustiati, Elly Lestari; Priyambodo, Priyambodo; Pratiwi, Dian Neli; Srihanto, Eko Agus; Lestari, Septi Wahyu; Sandra, Shifa; Winarno, Winarno; Thesalonika, Natasya
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.65548

Abstract

In response to environmental challenges and the critical imperative for sustainable progress, rural regions including agriculture practices are important in ecological robustness. It can be achieved through a synergistic relationship between rural development sustainability and the biological sciences. Concurrently, research in stewardship approaches are underway to amass data on robusta coffee, Coffea canephora, species. In collaboration with Lampung Disease Investigation Centre and local coffee farmers in Pesawaran, Lampung, and under DIPA BLU Universitas Lampung year 2024, initial ongoing work on coffee germ plasm data is done. Robusta coffee leaves were sampled from 2 blocks of traditional coffee plantations, Wiyono and Bogorejo, Pesawaran, Lampung.  Of 18 robusta leaves’ samples DNA extraction, electrophoresis showed 14 samples with good results. The optimum temperature for amplification is 62 °C and 63 °C.  The results will be sequenced for its species confirmation.  This project is expected to contribute bottom up towards inclusive and sustainable rural futures at the local level.
Sex Determination on Racing Pigeons (Columba livia) Molecularly Using Blood Samples Azzahra, Laila Salwa; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Febriyani, Dwi Ayu; Pratiwi, Dian Neli
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.785

Abstract

Background. Pigeons (Columba livia) belong to the Columbidae. Pigeons possess diverse ecological, economic, and aesthetic values, as they are monomorphic, exhibiting similar characteristics between male and female individuals, especially at a young age. Aims. In collaboration with the Lampung Disease Investigation Center, a study has been done to confirm the sex determination of pigeons at a young age by the PCR technique. Blood samples were taken in two locations, East Lampung and Kota Metro. Molecular análisis was carried out at the Biotechnology Laboratory, Lampung Disease Investigation Center. Methods. The technique includes DNA extraction, DNA amplification, electrophoresis and visualization. Result. It poses a significant challenge for pigeon breeders in sex determination. Blood is a source of genetic material that can be used to determine the sex of birds. The Chromo-Helicase-DNA-binding Protein (CHD) gene is one of the genes used as a marker to molecularly differentiate the sex of birds. The sex chromosomes in female individuals have heterozygous chromosomes (ZW) and male individuals have homozygous sex chromosomes (ZZ). Conclusion. Of eleven pigeon blood samples, there were seven female and four male individuals, and there was one mistake in the gender prediction by the pigeon owner
KAJIAN KARAKTER MORFOLOGIS LEBAH TANPA SENGAT PADA TUJUH KOLONI DI SIDO MULYO, PUNGGUR, LAMPUNG TENGAH Afandi, Aril; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Andriyani, Yuliana; Maharani, Annisa Lidya; Ashari, Minanti Mayda; Lestari, Septi Wahyu; Sandra, Shifa; Thesalonika, Natasya; Winarno, Winarno; Febriansyah, Muhammad; Pratiwi, Dian Neli
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 5 No. 1 (2025): April 2025
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v5i1.5983

Abstract

Stingless bees are an abundant natural resource in various areas of the world. The utilisation of stingless bees is associated with their honey production. Therefore, stingless bees are widely cultivated in the community. The increasing number of cultivators also occurs in Lampung Tengah Regency. However, this increase in the number of cultivators has not been followed by research on the diversity of stingless bees, including based on their morphology. This research was implemented from May to August 2024. Explorative fieldwork was performed in Sido Mulyo, Punggur, Lampung Tengah. According to the findings of the exploration, samples were taken from seven different colonies. The samples were then observed on 11 morphological characters including (1) eyes, (2) forewings, (3) body colour, (4) thorax, (5) abdomen, (6) hammuli, (7) mesonotum, (8) mesoscutetellum, (9) propodeum, (10) hair on posterior hind tibia, and (11) elliptical disc on basitarsus. The observed samples are closest to the characteristics of species in the genus Heterotrigona (samples SK3, SK5, SK6, and SM1) and Tetragonula (samples SK1, SK2, and SK4) on the basis of morphological characters.
Manifestation of Leaf Phenotype Character Diversity in Population Genetics Studies of Robusta Coffee (Coffea canephora) in the Gunung Betung Valley Titik Bogorejo Pesawaran Lampung: Manifestasi Keragaman Karakter Fenotipe Daun dalam Kajian Genetika Populasi Kopi Robusta (Coffea canephora) di Lembah Gunung Betung Titik Bogorejo Pesawaran Lampung Winarno, Winarno; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Lestari, Septi Wahyu; Sandra, Shifa; Thesalonika, Natasya; Ashari, Minanti Mayda; Andriyani, Yuliana; Maharani, Annisa Lidya; Afandi, Aril; Febriansyah, Muhammad; Pratiwi, Dian Neli; Permatasari, Nindy; Suhada, Suhada
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 1 (2025): BIO-EDU: Jurnal Pendidikan Biologi - April 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i1.8309

Abstract

Robusta coffee (Coffea canephora) genetic diversity is very important for breeding programmes aimed at increasing yield and adaptability. This diversity can be assessed through various morphological characters, especially leaf phenotype characters, which serve as indicators of genetic variation. This research was conducted from May to August 2024. Data collection was carried out by purposive sampling using the exploration and characterisation method of 30 coffee plants in the Traditional Coffee Plantation area, Bogorejo Village, Gedong Tataan District, Pesawaran Regency, Lampung Province. Qualitative character data collection was carried out modifying IPGRI descriptors. Based on the data obtained, there were no significant differences in qualitative characteristics and quantitative characteristics of leaves on 30 coffee plants. This indicates a low genetic diversity in the plantation.
AMPLIFIKASI GEN COI PADA SAMPEL LEBAH TANPA SENGAT DI LAMPUNG TENGAH: ANALISIS SECARA KUALITATIF Andriyani, Yuliana; Priyambodo, Priyambodo; Rustiaati, Elly Lestari; Pratami, Gina Dania; Ashari, Minanti Mayda; Srihanto, Eko Agus; Pratiwi, Dian Neli; Adelia, Ersa Imelda; Azzahra, Laila Salwa; Lestari, Septi Wahyu; Sandra, Shifa
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5539

Abstract

Indonesia memiliki keanekaragaman flora dan fauna yang tinggi dengan iklim tropis yang dimilikinya. Lebah tanpa sengat berhabitat di daerah tropis dan memiliki keanekaragaman yang tinggi dan menghasilkan produk madu dan propolis serta telah banyak dibudidayakan. Lampung Tengah sebagai salah satu daerah yang memiliki lokasi budidaya lebah tanpa sengat, dipilih sebagai lokasi penelitian. Penelitian telah dilakukan dengan analisis molekuler menggunakan penanda molekuler gen COI. Penelitian ini bertujuan untuk mengetahui kualitas hasil amplifikasi gen COI pada empat individu lebah tanpa sengat yang dikoleksi di Lampung Tengah. Analisis molekuler telah dilakukan melalui proses ekstraksi, amplifikasi, elektroforesis, dan visualisasi DNA. Kualitas DNA diketahui melalui pita DNA yang terbentuk pada proses elektroforesis gel agarosa 1% dan divisualisasikan di bawah sinar ultraviolet. Hasil amplifikasi gen COI pada empat individu lebah tanpa sengat memiliki kualitas pita DNA yang baik dengan panjang base pair berkisar antara 600 – 750bp.
ANALISIS HASIL AMPLIFIKASI SAMPEL INDIVIDU LEBAH TANPA SENGAT BERDASARKAN GEN COI DI LAMPUNG TIMUR Ashari, Minanti Mayda; Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratami, Gina Dania; Andriyani, Yuliana; Srihanto, Eko Agus; Pratiwi, Dian Neli; Azzahra, Laila Salwa; Adelia, Ersa Imelda; Sandra, Shifa; Lestari, Septi Wahyu; Ramadan, Viki
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5540

Abstract

Lebah tanpa sengat masuk ke dalam suku meliponidae dan merupakan lebah yang tidak menggunakan daya sengat sebagai alat pertahanan diri melainkan gigitan dan kerumunan apabila berada dalam bahaya. Indonesia memiliki keragaman jenis lebah tanpa sengat dan telah ditemukan sekitar 52 jenis yang tersebar di Pulau Sumatera, Kalimantan, Jawa, Timor, Sulawesi, Ambon, Maluku dan Irian Jaya. Dari beragam jenis lebah tanpa sengat yang ditemukan di Indonesia menunjukkan bahwa adanya keragaman genetik yang tinggi. Penelitian ini bertujuan untuk melakukan analisis hasil amplifikasi secara kualitatif dari DNA lebah tanpa sengat berdasarkan gen COI. Penelitian dilaksanakan pada rentang bulan Oktober 2024 - Januari 2025. Tahapan dalam penelitian ini meliputi pengambilan sampel, ekstraksi dan amplifikasi DNA. Hasil ekstraksi dan amplifikasi diuji dengan mengggunakan elektroforesis. Berdasarkan analisis kualitatif menggunakan gel agarose 1%, keempat sampel lebah tanpa sengat berhasil diekstraksi DNAnya. Ukuran panjang DNA dari masing-masing sampel lebah tanpa sengat diketahui berkisar antara 600-700bp dari hasil uji kualitatif setelah amplifikasi DNA.
UJI KEBERHASILAN AMPLIFIKASI GEN N-METHYLTRANSFERASE PADA KOPI ROBUSTA DI PERKEBUNAN KOPI RAKYAT LEMBAH GUNUNG BETUNG, BOGOREJO, LAMPUNG Sandra, Shifa; Rustiati, Elly Lestari; Priyambodo; Wahyuningsih, Sri; Srihanto, Eko Agus; Pratiwi, Dian Neli; Febriansyah, Muhammad; Lestari, Septi Wahyu; Thesalonika, Natasya; Winarno; Ashari, Minanti Mayda; Andriyani, Yuliana; Alfandi, Aril; Maharani, Annisa Lidya; Suhada, Suhada; Permatasari, Nindy
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5542

Abstract

Indonesia memiliki dua jenis kopi yang umum dibudidayakan, yaitu kopi robusta dan kopi arabika. Di Lampung, kopi robusta menjadi jenis kopi yang banyak ditanam. Masyarakat Lampung mayoritas melakukan budidaya di perkebunan tradisional. Keanekaragaman hayati pada kopi robusta secara genetik menggunakan analisis molekuler di perkebunan kopi rakyat masih belum banyak dilakukan. Tujuan dari penelitian ini untuk uji keberhasilan amplifikasi gen N-Methyltransferase pada kopi robusta di Perkebunan Kopi Rakyat Lembah Gunung Betung, Bogorejo, Lampung. Tahap penelitian ini meliputi ekstraksi DNA untuk mendapatkan DNA murni, mengetahui ukuran amplikon DNA melalui amplifikasi DNA menggunakan commercial kit, dan elektroforesis 1% gel agarosa. Hasil amplifikasi DNA pada lima sampel daun kopi robusta memperoleh pita DNA dengan ukuran berkisar antara 630-650 bp, tidak terdapat smear, namun terdapat variasi ketebalan pita DNA. Kata Kunci: N-methyltransferase, kopi robusta, Polymerase Chain Reaction
Co-Authors Adelia, Ersa Imelda Afandi, Aril Agus Subagyo Al Amin, Muhammadi Ridho Alfandi, Aril Alimudin, Salih Angga Kurniawan Ariyanto, Slamet Puji Arum, Setiawan Ashari, Minanti Mayda Azzahra, Laila Salwa Bayu , Raden Fadli Dwi Asmi Dwitama , Harry Putra Edi Santoso Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Elly Lestari, Rustiati Erdi Suroso Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Novianasari, Tika P. Haryanto, Sugeng Permatasari, Nindy Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Samsul , Bakri Sandora, Desta Sandra, Shifa Saswiyanti, Enny Sendora, Desta Septiana, Ana Sidik, Mahfud Sri Retnaning Rahayu, Sri Retnaning Suhada Suhada, Suhada Sunandar Sunandar Supriyadi, Tatang Susanto, Alvin Wiwiet Suwarno Thesalonika, Natasya Virnarenata, Elsa Wahyono, Agus WINARNO Winarno Winarno Winda, Indriyanti Yani, Ani Andri Yanti Yulianti Yanti, Yulianti Yuliana Andriyani