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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 6 Documents
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Search results for , issue "Vol. 17, No. 1" : 6 Documents clear
Effect of a Combination of Extract of Centella asiatica L. Leaves and Extract of Green Coffee (Coffea canephora robusta P.) Beans in a Cream Preparation for Grade 1-3 Cellulite and Slimming Primastuti, Riska Febriadne; Wih, Wong Lip; Mun’im, Abdul
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

An accumulation of fat in the subcutaneous tissue causes cellulite and dimpling on the surface of the skin. Although not related to obesity, obesity worsens cellulite. There are abundant topical anticellulite creams on the market, but the efficacy of these creams has not been scientifically proven. A combination of Centella asiatica L. leaves extract and green coffee (Coffea canephora robusta P.) bean extract in a cream preparation was clinically tested in 30 women for 84 days in the absence of diet and exercise. The descriptive-true experimental before (T-0)-after (T-84) method was used to classify the cellulite (grade 1–3) and to determine the slimming effect. The cellulite appearance and the body circumferences (abdominal and thigh) perimeters were photographed 5cm and 10cm below the navel and below the gluteal fold. The results of before and after the treatment showed that grade 1 cellulite lower (p < 0.000), the measurement of abdominal circumference showed reduction (p < 0.013), but the measurement of both thigh circumference showed insignificantly reduction (p < 0.512). The combination of both extracts reduce cellulite and fat deposits in the abdominal area, making the volunteers look slimmer
Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.
Effectiveness of Iron Fortification on Soy-Based Foods Using Ferrous Bisglycinate in the Presence of Phytic Acid Yunarti, Rika Tri; Zulys, Agustino; Harahap, Lina Yuliana; Pramukti, Mai Saroh Ambar
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

Determining the Source Parameters of the Jambi Earthquake (1 October 2009, Mw=6.4) Using Three-Component Local Waveforms Madlazim, Madlazim
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

The purpose of this research was to estimate the source parameters of a mainshock earthquake (Mw=6.4) that occurred on 1 October 2009 in the Dikit major segment of the Sumatran Fault Zone (SFZ). The source parameters were analyzed by the inversion of three-component local waveforms recorded by the GEOFON broadband IA network. Moment tensor of the event was determined using the Discrete Wave number method to calculate the Green function and the iterative deconvolution method to invert the moment tensors. From the analysis, we obtained the fault parameters of the mainshock, which are strike=324°, dip= 80° and rake= -173°. Abstra
Bioconcentration Factor of Copper (Cu), Lead (Pb), and Zinc (Zn) in Anadara indica Related to the Water Quality in Coastal Areas Takarina, Noverita Dian; Bengen, Dietriech Geoffrey; Sanusi, Harpasis Slamet; Riani, Etty
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

The Garapan river mouth forms part of the Tanjung Pasir coastal area. This region receives materials not only from coastal waters but also from domestic sewage and industrial wastewater that contains heavy metals such as Cu, Pb, and Zn. On the contrary, the Cibungur river mouth in the different coastal area, receives relatively lower amounts of domestic sewage and industrial wastewater. The objective of this study was to determine the Cu, Pb, and Zn content in the coastal sediment as well as the levels of A.indica and their relationship with water quality parameters. Samples were collected from four stations located in the Garapan and Cibungur river mouths and were analyzed for metal content using atomic absorption spectrometry Shimadzu 6300. Samples of A. indica found in the field are categorized into three diferent size classes. It is because size related to heavy metal accumulation. Results showed that bioconcentration factor (BCF) values were Zn>Cu>Pb in both locations. Small size A. indica tends to have higher values of BCF for Cu and Zn compared to medium and larger sized samples. Furthermore, the BCF values of Cu, Pb, and Zn in A. indica were higher when the sediment had a lower concentration of organic matter.
Purification of Biotransformation Products of Cis-Isoflavan-4-ol by Biphenyl Dioxygenase of Pseudomonas pseudoalcaligenes KF707 Strain Expressed in Escherichia coli Sulistiyani, Tri Ratna
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

Isoflavone has multiple beneficial effects on human health, especially through its antioxidant and anticancer activities. The biotransformation of isoflavone using byphenyl dioxygenase could be performed to extend the diversity of flavonoids and to improve their biological and physiological properties. Biotransformation of two enantiomers (3R, 4R)-cis-isoflavan-4-ol and (3S, 4S)-cis-isoflavan-4-ol by E. coli JM109 (pJHF108) carrying a biphenyl dioxygenase gene from P. pseudoalcaligenes KF707 produced two products, designated as CM1 and CM2. The products had a retention time of 11.9 and 14.6 min, respectively, and the same absorption peaks at 204, 220, and 275 nm. CM1 and CM2 had [M-H2O+H]+ at m/z 225. Based on the molecular mass and hydrolysis products, we proposed that epoxidation occurred on cis-isoflavan-4-ol. Chloroform extraction instead of ethyl acetate extraction was performed to improve the stability of cis metabolites, CM1 and CM2.

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