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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 5 Documents
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Search results for , issue "Vol. 17, No. 2" : 5 Documents clear
istribution Patterns of the Morphology, Species, and Sex in the Stingray Species Complex of Himantura uarnak, Himantura undulata, and Himantura leoparda in Indonesia Arlyza1, Irma Shita; Solihin, Dedy Duryadi; Soedharma, Dedi
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Abstract

The assessment of the morphology and stingray species distribution of the Himantura uarnak species complex covers eight sampling sites in western and eastern Indonesia as many as 113 individuals. The observation of the reticulate pattern was accomplished by directly checking the dorsal side of the stingrays, and growth observations also carried out. The distribution patterns were analyzed using the principal component analysis (PCA), while the growth patterns were evaluated using correlation analyses. The reticulation patterns of the H. uarnak species complex were highly variable, and the distribution patterns based on the morphological parameters, species, and sex produced four groups. The distribution of the length frequency of H. undulata and H. uarnak showed a range of different sizes. The body length of the female in the western region is relatively longer than the male, while in the eastern region the female revealed a range in relative size showing that the male was longer than the female. The length-weight relationships of the stingrays were negative allometric, indicating a b-value of less than 3. The b-values of both the male and female H. undulata were 1.5860 and 0.4380, respectively; while the male and female H. uarnak were 0.2956 and 0.4376, respectively.
Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families Yuliarni, Floreta Fiska; Sjamsuridzal, Wellyzar; Hidayat, Iman
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Abstract

Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of Cercospora spp. infecting the plant families of Asteraceae, Cucurbitaceae, and Solanaceae were determined in this study. The sequences of 30 strains of Cercospora spp. infecting the plant families of Fabaceae, Amaranthaceae, Apiaceae, Plumbaginaceae, Malvaceae, Cistaceae, Plantaginaceae, Lamiaceae, and Poaceae were obtained from GenBank. The molecular phylogenetic analysis revealed that the majority of Cercospora species lack host specificity, and only C. zinniicola, C. zeina, C. zeae-maydis, C. cocciniae, and C. mikaniicola were found to be host-specific. Closely related species of Cercospora could not be distinguished using molecular analyses of ITS, EF, and CAL gene regions. The topology of the phylogenetic tree based on the CAL gene showed a better topology and Cercospora species separation than the trees developed based on the ITS rDNA region or the EF gene.
Synthesis of Fe3O4 Nanoparticles from Ironstone Prepared by Polyethylene Glycol 4000 Astuti, Astuti; Claudia, Geby; Noraida, Noraida; Ramadhani, Melvira
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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This study reports the modification of the preparation method of Fe3O4 nanoparticles, which consists of two stages, beginning with the destruction and separation of iron ore from ironstone. Then, the Fe3O4 nanoparticles are synthesized using the coprecipitation method with magnetite (Fe3O4). Polyethylene glycol (PEG) 4000, a readily available chemical, was introduced in varying amounts into the reactions. The ratio of Fe3O4 powder and PEG 4000 is 1:3, 1:4, and 1:5, respectively, and the effects of the PEG 4000 on the morphology, crystalline size, and magnetic properties of the products were studied. It was shown that the particle and crystalline sizes decreased when the concentration of PEG 4000 increased. Additionally, the smallest Fe3O4 nanoparticles were around 50-60 nm, and semispherical nanoparticles were formed. The reduction of the crystalline size with the increase in PEG 4000 was shown by using XRD patterns, with the crystalline size being about 30 nm at a ratio of 1:5 Fe3O4 and PEG 4000, respectively. The hysteresis loop showed low coercivity, indicating that all products were soft magnetic.
Screening and Integration Analysis of OsDREB1A BC4F2 and BC5F1 Generations of Transgenic Ciherang Rice (Oryza sativa L.) for High-Salinity Tolerance Priyono, Dika Migi; Santoso, Tri Joko; Salamah, Andi
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Abstract

Salinity is one of a number of abiotic stresses that threaten rice production in Indonesia. To support food security programs, BB-Biogen has developed rice lines derived from OsDREB1A transformed Ciherang up to BC4F2 and BC5F1 generations. To verify the salinity tolerance and the stability of transgene integration, the BC4F2 and BC5F1 generations of Ciherang-OsDREB1A transgenic were screened to select high-salinity tolerant lines. Second, molecular analysis using the primers hptII-F/hptII-R and 35S-496-F/OsDREB1A-R was conducted to identify the existence and the stability of the transgene integration in the BC4F2 and BC5F1 generations. Screening 543 BC4F2 and BC5F1 Ciherang-OsDREB1A transgenic lines in a nutrient solution with a final electrical conductivity (EC) of approximately 18 mS/cm for 26 days yielded 134 putative transgenic plants. Integration analysis using the hptII-F/hptII-R primers showed that 73 of the 134 putative transgenic plants had positive PCR products, indicating the presence of the transgene in those plants. All the 73 plants also produced PCR products when tested with the specific primer 35S-496- F/OsDREB1A-R, indicating that transgene integration was maintained during the development of BC4F2 and BC5F1.
Design A Prototype of Temperature Logging Tools for Geothermal Prospecting Areas Supriyanto, Supriyanto; Mahmudah, Rizki; Marwah, Nella
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Abstract

The costs of geothermal exploration are very high because technology is still imported from other countries. The local business players in the geothermal sector do not have the ability to compete with global companies. To reduce costs, we need to develop our own equipment with competitive prices. Here in Indonesia, we have started to design a prototype of temperature logging tools for geothermal prospecting areas. This equipment can be used to detect temperature versus depth variations. To measure the thermal gradient, the platinum resistor temperature sensor is moved slowly down along the borehole. The displacement along the borehole is measured by a rotary encoder. This system is controlled by a 16-bit H8/3069F microcontroller. The acquired temperature data is displayed on a PC monitor using a Python Graphical User Interface. The system has been already tested in the Gunung Pancar geothermal prospect area in Bogor.

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