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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 5 Documents
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Search results for , issue "Vol. 17, No. 3" : 5 Documents clear
The Use of Lactic Acid Bacteria and Cellulolytic Bacteria to Improve the Chemical Properties of Corn Flour Rosyidah, Elly; Meryandini, Anja; Sunarti, Titi Candra
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Corn has the potential to be converted into flour, but its hard endosperm causies it to have a coarse particle size, and consequently, corn flour has high crude fiber, low protein, and high moisture. To overcome these problems, the milling of corn grain can be done by steeping it in water. The steeping process causes spontaneous growth of microbes, such as lactid acid bacteria and cellulolytic bacteria. The use of lactid acid bacteria and cellulolytic bacteria in the steeping process can soften the horny endosperm, which can enable corn grits to be ground into fine flours and which can improve the chemical properties of flour passed through an 80-mesh sieve. This study aimed to obtain isolates of indigenous lactid acid bacteria and cellulolytic bacteria from the corn steeping process, and to apply them as a starter in the steeping process of corn grits to improve the fine flour yield, and the chemical properties of flour passed through an 80-mesh sieve. Isolate E2213BAL was the lactid acid bacteria selected as the highest optical density at the logarithmic phase, while isolate B2121SLT was the cellulolytic bacteria with the highest enzyme activity (4.67 mU/ml). The results showed that the use of bacteria starter and fermentation time can decrease pH, increase total acid in the fermentation liquid, decrease the crude fiber, maintain protein, and decrease the moisture of corn flour passed through an 80-mesh sieve.
Isolation, Purification, and Characterization of Antimicrobial Substances from Endophytic Actinomycetes Sunaryanto, Rofiq; Mahsunah, Anis Herliyani
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. Plant samples were obtained from four different medicinal plants namely Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, and Symplocos cocchinensis. Isolation of actinomycetes was conducted using HV agar with the addition of cycloheximide, nystatin, nalidixic acid, and rifamycin. A total of 21 actinomycete isolates were obtained and tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, and Bacillus subtilis ATCC 66923. Among the isolates, isolate KY01 was the most active to Gram-positive and Gram-negative bacteria. Morphological observation and identification using 16S rRNA gene sequence showed that the isolate KY01 was similar to Streptomyces antibioticus. An active compound from the isolate KY01 was produced using yeast peptone medium. The active compound was purified using silica-gel-column chromatography and preparative high performance liquid chromatography (HPLC). A single peak of the active compound was detected with HPLC and LCMS, which showed a retention time of 26.6 min and molecular weight (MW) 906.4474 g/mol, respectively.
One-Pot Transformation of (R)-(+)-Citronellal over a Bifunctional Ni/ZnBr2/β-Zeolite Catalyst Nisyak, Khoirun; Iftitah, Elvina Dhiaul; Tjahjanto, Rachmat Triandi
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Solution of Dirac Equations for Cotangent Potential with Coulomb-type Tensor Interaction for Spin and Pseudospin Symmetries Using Romanovski Polynomials Cari, Cari; Deta, Utama Alan; Werdiningsih, Intan Saraswati
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Solution of Dirac Equations for Cotangent Potential with Coulomb-type Tensor Interaction for Spin and Pseudospin Symmetries Using Romanovski Polynomials
Isolation and Identification of Airborne Bacteria Inside Swiftlet Houses in Sarawak, Malaysia Sien, Leong Sui; Chuan, Chia Hwa; Lihan, Samuel; Yee, Ling Teck
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Air consists of such microorganisms as bacteria, fungi, and viruses. Exposure to these airborne bacteria indoors may cause infectious and noninfectious adverse health effects. However, the sources and origins of bacteria are not fully understood. The aim of this study was to isolate and identify the bacteria present in the air inside swiftlet houses located in Kota Samarahan, Saratok, Betong, Maludam, Miri, Kuching, Semarang, Sepinang, Sarikei, and Sibu in Sarawak, Malaysia. A total of 100 bacterial isolates from 20 samples were collected from swiftlet houses. The bacteria present in the air were collected using Plate Count Agar. Two plates were exposed at the front and back inside swiftlet houses for 15 sec and then incubated at 37 ± 1 oC for 24 h. The 16S rRNA analysis method was used to identify the isolates from the samples. The air inside the swiftlet houses had a total mean airborne bacteria colony count of 2.02 ± 0.72 log10 cfu/m2 /sec; the highest was in Miri (3.08 ± 0.29 log10 cfu/m2 /sec), and the lowest was in Sibu (1.05 ± 0.85 log10 cfu/m2 /sec). Twenty-seven bacteria species were identified, and Lysinibacillus sp. B4 (16%) was most frequently isolated

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