cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ivandini Tribidasari A.
Contact Email
ivandini.tri@sci.ui.ac.id
Phone
+622129120943
Journal Mail Official
editor_mss@ui.ac.id
Editorial Address
Directorate of Research and Community Engagement UNIVERSITAS INDONESIA UI Campus, Depok 16424 Indonesia
Location
Kota depok,
Jawa barat
INDONESIA
Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol. 19, No. 4" : 6 Documents clear
Adsorption of Phosphate Ion in Water with Lithium-Intercalated Gibbsite Sihombing, Riwandi; Krisnandi, Yuni Krisyuningsih; Widya, Rahma; Luthfiyah, Siti Zahrotul; Yunarti, Rika Tri
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In order to enhance adsorption capacity of gibbsite (Al(OH)3 as an adsorbent for the adsorption of phosphate in water, gibbsite was modified through lithium-intercalation. The purification method of Tributh and Lagaly was applied prior to intercalation. The Li-Intercalation was prepared by the dispersion of gibbsite into LiCl solution for 24 hours. This intercalation formed an cationic clay with the structure of [LiAl2(OH)6]+ and exchangeable Cl- anions in the gibbsite interlayer. A phosphate adsorption test using Lithium-intercalated gibbsite (LIG) resulted in optimum adsorption occurring at pH 4.5 with an adsorption capacity of 11.198 mg phosphate/g LIG which is equivalent with 1.04 wt% LIG. The adsorption capacity decreased with decreasing amounts of H2PO4-/HPO4- species in the solution. This study showed that LIG has potential as an adsorbent for phosphate in an aqueous solution with pH 4.5–9.5.
Comparison of Immobilized Metal Affinity Chromatography Ni-NTA and Co-TALON for the Purification of Recombinant Human Erythropoietin Rubiyana, Yana; Santoso, Adi; Batubara, Irmanida
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on the imidazole ring, histidine is often used in the IMAC purification system. Two types of commercial IMAC resin use a nitrilotriacetic acid (NTA) matrix: a nickel-based (Ni-NTA) and cobalt-based (Co-NTA), better known as TALON. This study was aim to investigate the effect of the metal ions Ni2+ and Co2+ to purify recombinant human erythropoietin (rhEPO) expressed in yeast system Pichia pastoris. The results indicated that both Ni-NTA and Co-TALON gave almost the same level of protein purity; however, Ni-NTA has a higher binding affinity than Co-TALON might be due to the higher stability complex of Ni+. The average amount of protein bound by Ni-NTA and Co-TALON was 183.5 and 38.7 µg/mL, respectively.
Co-digestion of Sorghum Stalk and Sludge for Biogas Production Romli, Muhammad; Anggraini, Aulia; Purwoko,; Suprihatin,
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The recycling of residual agricultural biomass using anaerobic digestion allows for the recovery of biomass carbon and nutrients as sources of energy and fertilizer. The obstacles that are encountered in this process include the lignocellulosic structure of biomass tissue and its high carbon-to-nitrogen (C:N) ratio. This study evaluates the co-digestion system of pretreated sorghum stalks and wastewater sludge. The stalks were pretreated by partial bio-oxidation to improve their bacterial accessibility. The digesters were fed a mixture of stalk and sludge at ratios of 100:0, 80:20, 60:40, and 40:60 (total solids [TS] basis). The digesters were run in batches at 35-36 °C, with an initial TS of 15%. The digesters’ performance was evaluated in terms of biogas production rate and yield. The digesters that were run with feed ratios of 80:20 and 60:40 showed shorter lag phase, higher biogas generation rates, and higher biogas yields compared to those run with feed ratios of 100:0 and 40:60. The highest specific biogas production (of 122 L/kg TS) was achieved by the digesters run at ratios of 80:20 and 60:40. The digesters run only with stalks (ratio 100:0) resulted in specific gas production of 67 L/kg TS, whereas those fed on a feed ratio of 40:60 generated only 13 L/kg TS. We conclude that the co-digestion of sorghum stalks and wastewater sludge at a proper ratio improves biogas production.
Population Status and Habitat Preferences of Critically Endangered Dipterocarpus littoralis in West Nusakambangan, Indonesia Robiansyah, Iyan; Davy, Anthony John
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The conservation of the endemic tree species Dipterocarpus littoralis (Bl.) Kurz. is hampered by the paucity of information on its population biology and ecology. Consequently, a targeted survey was carried out in the West Nusakambangan Nature Reserve to assess its population size and structure as well as habitat preferences. In total, 676 individuals of D. littoralis were located at 52 locations, with an extent of occurrence of 3.66 km2 and an area of occupancy of 1.71 km2. The population had an inverse-J-shaped distribution of diameter at breast height (DBH), with 63% of individuals in the 0-5 cm class and another 21% in the 5-10 cm class; only 11 (1.6%) mature individuals (DBH≥30) were found. D. littoralis was associated with steep, low, southwest-facing sites and sites that had high litter cover and thickness. Illegal logging and fuel-wood chopping were the main threats to D. littoralis and its habitat. In addition, an invasive shrub, Langkap (Arenga obtusifolia, Arecaceae), was a potential competitor with the seedlings throughout the reserve. In view of its endemism, narrow range and localized distribution, small population, environmental preferences, and the severe threats from anthropogenic activities and invasive species, D. littoralis appears to more than justify its conservation status of Critically Endangered.
Microfungi on Leaves of Licuala bidentata (Arecaceae) from Sarawak, Malaysia Lateef, Adebola; Muid, Sepiah; Bolhassan, Mohamad Hasnul
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A microfungal survey was carried out on the living leaves and litters of Licuala bidentata in Kubah National Park in Sarawak, Malaysia. A total of 400 leaf segments (200 segments for each leaf type) were plated on two isolation media (water agar and malt extract agar) for endophytic and saprophytic fungal isolation. Forty-three microfungal species were obtained from both leaf types, 31 species identified from living leaves and 18 species from litters. Only six species were common to both leaf types, with 25 and 12 species exclusively identified from living leaves and litters, respectively. New records of fungi from this host plant and for the genus Licuala include Isthmotricladia laeensis, Chloridium sp., Mucor sp., Oidiodendron sp., Kinochaeta sp., Cryptophiale sp., Chrysosporium merdarium and Circinotrichum fertile. This study constitutes the first report on microfungal community on L. bidentata. Implications of this new report in comparison with the microfungal species on other plant species in the genus Licuala are discussed.
In Vitro Formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2'-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone Budiawan,; Widiastuti, Dwi Retno
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2¢-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2¢-deoxyguanosine (dG). PG in the presence of CuCl2 increased the 8-OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl2 at 1.28×10-5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20–150 ppm) ranged from 75.50 to 312.06 8-OHdG per 105 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl2 by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M+. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9.

Page 1 of 1 | Total Record : 6

 1 

Filter by Year

2015 2015


Reset
Filter By Issues
All Issue Vol. 29, No. 3 Vol. 29, No. 2 Vol. 29, No. 1 Vol. 28, No. 4 Vol. 28, No. 3 Vol. 28, No. 2 Vol. 28, No. 1 Vol. 27, No. 4 Vol. 27, No. 3 Vol. 27, No. 2 Vol. 27, No. 1 Vol. 9, No. 1 Vol. 26, No. 4 Vol. 26, No. 3 Vol. 26, No. 2 Vol. 26, No. 1 Vol. 25, No. 4 Vol. 25, No. 3 Vol. 25, No. 2 Vol. 25, No. 1 Vol. 24, No. 4 Vol. 24, No. 3 Vol. 24, No. 2 Vol. 24, No. 1 Vol. 23, No. 4 Vol. 23, No. 3 Vol. 23, No. 2 Vol. 23, No. 1 Vol. 22, No. 4 Vol. 22, No. 3 Vol. 22, No. 2 Vol. 22, No. 1 Vol. 12, No. 1 Vol. 21, No. 4 Vol. 21, No. 3 Vol. 21, No. 2 Vol. 21, No. 1 Vol. 20, No. 4 Vol. 20, No. 3 Vol. 20, No. 2 Vol. 20, No. 1 Vol. 19, No. 4 Vol. 19, No. 3 Vol. 19, No. 2 Vol. 19, No. 1 Vol. 18, No. 4 Vol. 18, No. 3 Vol. 18, No. 2 Vol. 18, No. 1 Vol. 17, No. 3 Vol. 17, No. 2 Vol. 17, No. 1 Vol. 16, No. 3 Vol. 16, No. 2 Vol. 16, No. 1 Vol. 15, No. 2 Vol. 15, No. 1 Vol. 14, No. 2 Vol. 14, No. 1 Vol. 13, No. 2 Vol. 13, No. 1 Vol. 12, No. 2 Vol. 11, No. 2 Vol. 11, No. 1 Vol. 10, No. 2 Vol. 10, No. 1 Vol. 9, No. 2 Vol. 8, No. 3 Vol. 8, No. 2 Vol. 8, No. 1 Vol. 7, No. 3 Vol. 7, No. 2 Vol. 7, No. 1 Vol. 6, No. 3 Vol. 6, No. 2 Vol. 6, No. 1 More Issue