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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 15 Documents
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Search results for , issue "Vol. 30, No. 1" : 15 Documents clear
Phytochemistry and Pharmacological Activities of Sphagneticola trilobata: A Systematic Review with Perspectives for Drug Discovery Labhade, Sonali Dnyaneshwar; Kamadi, Suraj; Karwa, Pawan Nandkishor; Bhole, Ritesh Prakash; Kapare, Harshad Sunil; Chitlange, Sohan Satyanarayan
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

Sphagneticola trilobata (L.) Pruski is a medicinal plant widely used in traditional medicine for its anti-inflammatory, antimicrobial, antioxidant, hepatoprotective, wound healing, and CNS depressant effects. Despite its broad pharmacolo-gical potential, clinical application is limited due to a lack of mechanistic understanding, standardization, and clinical validation. This review aims to provide a comprehensive overview of the phytochemical composition, pharmacological activities, and therapeutic potential of S. trilobata, while highlighting research gaps and suggesting future directions for clinical translation. A systematic review of scientific literature, ethnobotanical sources, and pharmacological studies was conducted, focusing on phytochemical analysis, biological activity, toxicity, and potential applications in evidence-based medicine. Phytochemical studies reveal that S. trilobata contains flavonoids, terpenoids, sesquiterpene lactones, and phe-nolic compounds. Key constituents such as kaurenoic acid, grandiflorenic acid, and wedelolactone are associated with antioxidant, anti-inflammatory, hepatoprotective, antimicrobial, antidiabetic, and anticancer properties. However, there are gaps in understanding its pharmacokinetics, mechanisms of action, and clinical relevance. Although S. trilobata shows strong pharmacological promise, further research is needed to clarify molecular mechanisms, enhance bioavailability, and conduct well-structured clinical trials. These efforts are essential to support its integration into evidence-based and modern medicinal systems.
Dietary Oil from Hermetia illucens L. Larvae Promotes Weight Gain and Alters Leukocyte Profiles in Mus musculus L Nugroho, Rudy Agung, RAN; Kurniati, Reni; Sari, Widya Putri Ambar; Sompotan, Reynaldo Imanuel; Aryani, Retno; Manurung, Hetty; Rudianto, Rudianto
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

The escalating global demand for high-quality animal-derived proteins has driven the search for sustainable alternatives. Black soldier fly larvae (BSFL; Hermetia illucens L.) oil presents a promising option, by addressing organic waste management while providing nutrient-rich feed. This study evaluated the effects of dietary supplementation with BSFL oil on growth performance, hematological characteristics, and blood lipid profiles in mice (Mus musculus). Male mice were randomly assigned to five groups (n = 5): positive control (fish oil, 5 mL/kg body weight [BW]), negative control (Aquadest, 5 mL/kg BW), and three treatment groups receiving BSFL oil at 2.5, 5, and 10 mL/kg BW, respectively. Interventions were administered orally for 28 days; assessments included BW, hematological parameters, and lipid profiles. The results indicated that 10 mL/kg BW BSFL oil significantly increased BW (34.40 ± 0.74 g) compared to the fish oil group (31.6 ± 0.67 g, p < 0.05). Hematological parameters and lipid profiles did not differ significantly across groups (p > 0.05). However, leukocyte profiles exhibited significant variations (p < 0.05), particularly at 10 mL/kg BW of BSFL oil, suggesting immunomodulation. This preliminary proof-of-concept study in mice provides the first head-to-head comparison of unrefined BSFL and commercial fish oil utility, offering mechanistic insights applicable to livestock nutrition
Phenol–Chloroform DNA Extraction for DNA Amplification from Personal Items Ernanto, Aditya Rahman; Khasanah, Dinda Amaliyatul; Aldirahayu, Yunita; Setiyaningrum, Rizki; Iswara, Arya
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

This study examined the feasibility of extracting DNA from various personal items using the phenol–chloroform–isoamyl alcohol (PCIA) method. A towel, collared shirt, toothbrush, shower puff, comb, hair tie, and buccal swabs were analyzed. The quality of the DNA extraction, along with DNA concentration, purity, and suitability for multiplex PCR amplification, were evaluated. The shower puff exhibited the highest DNA concentration (68.35 ng/µL), whereas the collared shirt yielded the lowest (26.43 ng/µL). All samples exhibited good DNA purity (A260 nm/A280 nm ranging from 1.827 to 1.985), emphasizing the potential of this method for forensic analysis. Factors influencing DNA concentration included material composition, surface area, and exposure to bodily fluids. The results highlight the importance of understanding material-specific characteristics to optimize DNA extraction protocols. Buccal swabs served as reliable controls, suggesting their potential use in personal item DNA extraction studies. The successful extraction of DNA from personal items holds promise for enhancing the scope of DNA analysis in criminal investigations. The PCIA method is useful for DNA extraction from personal items and may serve as an alternative when standard methods cannot be used.
Lab-made 100 bp DNA Ladder using Polymerase Chain Reaction and Human DNA Bariqi, Muhammad Ilmam; Yamamoto, Zulham; Firjatu, Putri Chalya; Nasution, Luthfi Umam Hakim; Lubis, Oryza Sativa
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

Polymerase chain reaction (PCR) is a rapid, molecular biology technique widely used in disease diagnosis and genetic engineering. Conventional PCR products require agarose gel electrophoresis, which employs a DNA ladder as a size reference. Most commercial ladders are plasmid-based and reliable but require additional culture time. We suggest a more efficient method for producing a DNA ladder using DNA derived from human blood. DNA was isolated using a commercial kit. Primer sets generating 100–1000 base pair (bp)-long fragments bearing target regions p12, p13, and p14 were designed using Primer-BLAST. DNA was amplified by routine PCR, visualized on a 1% (w/v) agarose gel, and pooled to form a 100-bp ladder. The lab-made ladder was compared with a commercial ladder. Extracted DNA showed a purity of 1.878. Bands of 100, 500, and 1000 bp were added in greater amounts to produce wider, more visible bands. The pooled fragments served as a functional 100-bp DNA ladder. A key limitation is the lack of sequencing, as genomic variations may affect flanking regions and potentially alter amplicon sizes. A 100-bp DNA ladder generated using DNA extracted from human blood had good quality, comparable to that of a commercial ladder.
Liparis condylobulbon Reichb. f. from Gumitir, Indonesia: Morphology, Molecular, and Phytochemical Profile Ulum, Fuad Bahrul; Arum, Asyifa Yasmin; Sa’adah, Kurnia; Setyati, Dwi; Dwinianti, Edia Fitria; Rajamani, Ranjithkumar; Nugraha, Ari Satia; Su’udi, Mukhamad
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

Liparis condylobulbon Rchb.f. is an epiphytic orchid across South and Southeast Asia, which holds notable ethnomedicinal and pharmacological significance. This study aims to characterize Indonesian Liparis condylobulbon using integrated morphological, molecular (matK, rbcL, ITS2), and phytochemical (LC-MS, DPPH) analyses of specimens from Mount Gumitir, providing the first comprehensive taxonomic and pharmacological profile to support species authentication and future bioprospecting. The L. condylobulbon specimen displays diagnostic epiphytic morpho-logy, including fusiform pseudobulbs, two elongate leaves, and an erect inflorescence bearing ~123 flowers. Molecular barcoding using matK, rbcL, and ITS2 confirmed its identity, with phylogenetic analyses consistently clustering it with L. condylobulbon, supporting taxonomic placement despite minor ITS2-based variation. Metabolomic analysis revealed 58 distinct secondary metabolites, including 30 flavonoids, 22 terpenoids, and 6 phenolic compounds, with key bioactives such as diosmin, picrasinoside B, and 6-gingerol exhibiting known anti-inflammatory, antioxidant, and anticancer properties. Although the crude extract showed relatively low antioxidant capacity (IC₅₀ = 650 µg/mL), the chemical diversity and presence of pharmacologically active constituents underscore the species' potential for future phytotherapeutic applications.

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