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Ali Rahmat
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Daerah istimewa yogyakarta
INDONESIA
Applied Research in Science and Technology
ISSN : 27767213     EISSN : 27767205     DOI : -
Applied Research in Science and Technology is a peer-reviewed open-access journal which publishes result from scientists and engineers in many fields of science and technology. Every submitted manuscript will be reviewed by at least two peer-reviewers using the double-blind review method. focus and scope: Infrastructures Technology, Remote Sensing and Geospatial Information System, Soil Science, Geology, Marine Science, Technology and Engineering, Electrical, Biomedical Engineering, Renewable Energy, Physics, Chemistry, Materials, Biology, Forestry, Agriculture, Earth & Space Science.
Articles 11 Documents
Search results for , issue "Vol. 5 No. 1 (2025): Applied Research in Science and Technology" : 11 Documents clear
Application of Biosecurity Strategies for the Control of MAS (Motile Aeromonas Septicemia) in Tubifex Worms (Tubifex sp.) for Larval Catfish (Pangasius sp.) Culture Firdausi, Amalia Putri; Indriastuti, Cecilia Eny; Surahman, Nur Prasetyo Ari; Permatasari, Sheny
Applied Research in Science and Technology Vol. 5 No. 1 (2025): Applied Research in Science and Technology
Publisher : Research and Social Study Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33292/areste.v5i1.90

Abstract

Background: Catfish (Pangasius sp.) is one of the high-value freshwater aquaculture commodities in Indonesia. To ensure sustainable production, hatchery management plays a crucial role, as the larval and juvenile stages are the most vulnerable to environmental stress and disease outbreaks. During the larval phase (7–14 days post-hatch), Tubifex worms (Tubifex sp.) are commonly used as a natural feed due to their high nutritional content and digestibility.Aims & Methods: This study aimed to evaluate the effectiveness of formalin in suppressing Aeromonas hydrophila in Tubifex worms (Tubifex sp.), which are used as natural feed for larval catfish (Pangasius sp.). The research was conducted in two phases: in vitro and in vivo assays. The in vitro assay was carried out to determine the minimum inhibitory concentration (MIC) and inhibition zone of formalin against A. hydrophila. The in vivo assay consisted of two parts: a toxicity test of formalin on Tubifex worms and an evaluation of the effect of treated worms as feed on the survival of catfish larvae. A completely randomized design (RAL) was employed, consisting of four treatments with three replicates each: K (control—untreated Tubifex), A (400 ppm formalin immersion without rinsing), B (400 ppm formalin immersion with one rinse), and C (400 ppm formalin immersion with two rinses).Result: The results demonstrated that a 400 ppm formalin concentration effectively inhibited the growth of A. hydrophila. Treatment C (two rinses following immersion in 400 ppm formalin) significantly reduced the toxic effects of formalin on the Tubifex worms used as natural feed. Consequently, this treatment led to an improvement in the survival rate of catfish larvae, reaching 44.6 ± 11.5% over a 14-day rearing period.

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